Capsaicin Treatment Attenuates Cholangiocarcinoma Carcinogenesis
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{"title"=>"Capsaicin treatment attenuates cholangiocarcinoma carcinogenesis", "type"=>"journal", "authors"=>[{"first_name"=>"Annika", "last_name"=>"Wutka", "scopus_author_id"=>"56145303400"}, {"first_name"=>"Vindhya", "last_name"=>"Palagani", "scopus_author_id"=>"36462675800"}, {"first_name"=>"Samarpita", "last_name"=>"Barat", "scopus_author_id"=>"56145323500"}, {"first_name"=>"Xi", "last_name"=>"Chen", "scopus_author_id"=>"56145564200"}, {"first_name"=>"Mona", "last_name"=>"El Khatib", "scopus_author_id"=>"55229454100"}, {"first_name"=>"Julian", "last_name"=>"Götze", "scopus_author_id"=>"56145151600"}, {"first_name"=>"Hanane", "last_name"=>"Belahmer", "scopus_author_id"=>"25421549700"}, {"first_name"=>"Steffen", "last_name"=>"Zender", "scopus_author_id"=>"24402275400"}, {"first_name"=>"Przemyslaw", "last_name"=>"Bozko", "scopus_author_id"=>"6507858927"}, {"first_name"=>"Nisar P.", "last_name"=>"Malek", "scopus_author_id"=>"6701536363"}, {"first_name"=>"Ruben R.", "last_name"=>"Plentz", "scopus_author_id"=>"6602207506"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84899688700", "pui"=>"372991133", "pmid"=>"24748170", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0095605", "sgr"=>"84899688700"}, "id"=>"72176864-ce50-3401-845e-1f7633d735a6", "abstract"=>"Capsaicin, the most abundant pungent molecule produced by pepper plants, represents an important ingredient in spicy foods consumed throughout the world. Studies have shown that capsaicin can relieve inflammation and has anti-proliferative effects on various human malignancies. Cholangiocarcinoma (CC) is a cancer disease with rising incidence. The prognosis remains dismal with little advance in treatment. The aim of the present study is to explore the anti-tumor activity of capsaicin in cultured human CC cell lines. Capsaicin effectively impaired cell proliferation, migration, invasion, epithelial to mesenchymal transition and growth of softagar colonies. Further, we show that capsaicin treatment of CC cells regulates the Hedgehog signaling pathway. Conclusion: Our results provide a basis for capsaicin to improve the prognosis of CCs in vivo and present new insights into the effectiveness and mode of action of capsaicin.", "link"=>"http://www.mendeley.com/research/capsaicin-treatment-attenuates-cholangiocarcinoma-carcinogenesis", "reader_count"=>10, "reader_count_by_academic_status"=>{"Researcher"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>3, "Student > Bachelor"=>3}, "reader_count_by_user_role"=>{"Researcher"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>3, "Student > Bachelor"=>3}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Materials Science"=>1, "Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>2, "Sports and Recreations"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Sports and Recreations"=>{"Sports and Recreations"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}}, "reader_count_by_country"=>{"India"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1553131"], "description"=>"<p>The expression levels of Smo, Gli1 and Gli2 were analyzed by semiquantitative RT-PCR both in SZ-1 (A) and in TFK-1 cells (B) after treatment either with control (DMSO) or capsaicin (150 µM, 200 µM) for 24 h, 48 h and 96 h. A reduction of transmembrane protein Smo was seen in both cell lines after 96 h. Capsaicin down-regulates Hedgehog targets Gli1 and Gli2 in a time-dependent manner (96 h).</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "Oncogenic signaling", "Molecular cell biology", "Complementary and alternative medicine", "Gastroenterology and hepatology", "Gastrointestinal cancers", "oncology", "Cancers and neoplasms", "Gastrointestinal tumors", "cholangiocarcinoma", "Basic cancer research", "Cancer treatment", "targets", "hedgehog"], "article_id"=>1073133, "categories"=>["Biological Sciences"], "users"=>["Annika Wutka", "Vindhya Palagani", "Samarpita Barat", "Xi Chen", "Mona El Khatib", "Julian Götze", "Hanane Belahmer", "Steffen Zender", "Przemyslaw Bozko", "Nisar P. Malek", "Ruben R. Plentz"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095605.g007", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Capsaicin_targets_Hedgehog_signaling_/1073133", "title"=>"Capsaicin targets Hedgehog signaling.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-18 10:01:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/1553064"], "description"=>"<p>SZ-1 (A) and TFK-1 (B) cells were treated with capsaicin or DMSO as control for 24 h, 48 and 96 h. Viable cells were collected and were stained by Annexin V/PI dye. The stained cells were determined by flow cytometry. Data are representing the results of two independent experiments. Data are expressed as mean ± SD of triplicates.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "Oncogenic signaling", "Molecular cell biology", "Complementary and alternative medicine", "Gastroenterology and hepatology", "Gastrointestinal cancers", "oncology", "Cancers and neoplasms", "Gastrointestinal tumors", "cholangiocarcinoma", "Basic cancer research", "Cancer treatment", "apoptosis"], "article_id"=>1073066, "categories"=>["Biological Sciences"], "users"=>["Annika Wutka", "Vindhya Palagani", "Samarpita Barat", "Xi Chen", "Mona El Khatib", "Julian Götze", "Hanane Belahmer", "Steffen Zender", "Przemyslaw Bozko", "Nisar P. Malek", "Ruben R. Plentz"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095605.g002", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Capsaicin_induces_apoptosis_of_cholangiocarcinoma_cells_/1073066", "title"=>"Capsaicin induces apoptosis of cholangiocarcinoma cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-18 10:01:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/1553157", "https://ndownloader.figshare.com/files/1553158", "https://ndownloader.figshare.com/files/1553159", "https://ndownloader.figshare.com/files/1553160"], "description"=>"<div><p>Capsaicin, the most abundant pungent molecule produced by pepper plants, represents an important ingredient in spicy foods consumed throughout the world. Studies have shown that capsaicin can relieve inflammation and has anti-proliferative effects on various human malignancies. Cholangiocarcinoma (CC) is a cancer disease with rising incidence. The prognosis remains dismal with little advance in treatment. The aim of the present study is to explore the anti-tumor activity of capsaicin in cultured human CC cell lines. Capsaicin effectively impaired cell proliferation, migration, invasion, epithelial to mesenchymal transition and growth of softagar colonies. Further, we show that capsaicin treatment of CC cells regulates the Hedgehog signaling pathway. <i>Conclusion:</i> Our results provide a basis for capsaicin to improve the prognosis of CCs <i>in vivo</i> and present new insights into the effectiveness and mode of action of capsaicin.</p></div>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "Oncogenic signaling", "Molecular cell biology", "Complementary and alternative medicine", "Gastroenterology and hepatology", "Gastrointestinal cancers", "oncology", "Cancers and neoplasms", "Gastrointestinal tumors", "cholangiocarcinoma", "Basic cancer research", "Cancer treatment", "attenuates"], "article_id"=>1073149, "categories"=>["Biological Sciences"], "users"=>["Annika Wutka", "Vindhya Palagani", "Samarpita Barat", "Xi Chen", "Mona El Khatib", "Julian Götze", "Hanane Belahmer", "Steffen Zender", "Przemyslaw Bozko", "Nisar P. Malek", "Ruben R. Plentz"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0095605.s001", "https://dx.doi.org/10.1371/journal.pone.0095605.s002", "https://dx.doi.org/10.1371/journal.pone.0095605.s003", "https://dx.doi.org/10.1371/journal.pone.0095605.s004"], "stats"=>{"downloads"=>11, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Capsaicin_Treatment_Attenuates_Cholangiocarcinoma_Carcinogenesis_/1073149", "title"=>"Capsaicin Treatment Attenuates Cholangiocarcinoma Carcinogenesis", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-04-18 10:01:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/1553088"], "description"=>"<p>Treatment with capsaicin suppresses the migration potential of human cholangiocarcinoma cell lines SZ-1 and TFK-1. Wound healing experiments of (A) SZ1 and (B) TFK cells cultured with capsaicin (150 µM, 200 µM) or control (DMSO). A scratch was made at (time 0 h) in both SZ-1 and TFK-1 and maintained for 24 h in conditioned medium with capsaicin or DMSO. The dotted lines are representing the edges of the wound. Photographs were taken under light microscope (10× magnification). After 24 h (A) SZ1 and (B) TFK-1 showed significant inhibition under 150 µM and 200 µM capsaicin. In DMSO treated cells 90% of the wound healing was observed after 24 hrs. (A,B) The migration index was calculated as described in Material and Methods and plotted in bar graphs. P values were calculated with ANOVA analysis of variance along with Bonferroni post test. The error bar represents standard deviation. Differences were considered as statistically significant (*) when the P-value was less <0.05. Data are expressed as mean ± SD of triplicates.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "Oncogenic signaling", "Molecular cell biology", "Complementary and alternative medicine", "Gastroenterology and hepatology", "Gastrointestinal cancers", "oncology", "Cancers and neoplasms", "Gastrointestinal tumors", "cholangiocarcinoma", "Basic cancer research", "Cancer treatment", "attenuates"], "article_id"=>1073090, "categories"=>["Biological Sciences"], "users"=>["Annika Wutka", "Vindhya Palagani", "Samarpita Barat", "Xi Chen", "Mona El Khatib", "Julian Götze", "Hanane Belahmer", "Steffen Zender", "Przemyslaw Bozko", "Nisar P. Malek", "Ruben R. Plentz"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095605.g003", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Capsaicin_attenuates_migration_of_human_cholangiocarcinoma_cells_/1073090", "title"=>"Capsaicin attenuates migration of human cholangiocarcinoma cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-18 10:01:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/1553051"], "description"=>"<p>The cell proliferation of (A) SZ1 and (C) TFK-1 cells was measured by cell proliferation assay. Capsaicin (150 µM, 200 µM) inhibited cell proliferation in a dose- and time-dependent manner. Light microscopic pictures (10× magnification) were taken at 96 h to show the effect of capsaicin on cell proliferation of (B) SZ1 and (D) TFK-1. Note that these results reveal the anti-proliferative effects of capsaicin on human cholangiocarcinoma cells. Data are expressed as mean ± SD of triplicates.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "Oncogenic signaling", "Molecular cell biology", "Complementary and alternative medicine", "Gastroenterology and hepatology", "Gastrointestinal cancers", "oncology", "Cancers and neoplasms", "Gastrointestinal tumors", "cholangiocarcinoma", "Basic cancer research", "Cancer treatment", "inhibits", "proliferation"], "article_id"=>1073053, "categories"=>["Biological Sciences"], "users"=>["Annika Wutka", "Vindhya Palagani", "Samarpita Barat", "Xi Chen", "Mona El Khatib", "Julian Götze", "Hanane Belahmer", "Steffen Zender", "Przemyslaw Bozko", "Nisar P. Malek", "Ruben R. Plentz"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095605.g001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Capsaicin_inhibits_cell_proliferation_in_human_cholangiocarcinoma_cell_lines_/1073053", "title"=>"Capsaicin inhibits cell proliferation in human cholangiocarcinoma cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-18 10:01:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/1553114"], "description"=>"<p>SZ-1 (A) and TFK-1 (A) cell lines were treated for 48 h with control (DMSO) and capsaicin (150 µM, 200 µM) to investigate the effect of capsaicin on invasiveness of human cholangiocarcinoma cells. The number of cells that invaded through the membrane was determined by light microscope (20× magnification) counterstained and invasion index (A,B) was calculated as described in Material and Methods and plotted in bar graphs. Both TFK-1 and SZ-1 showed significant decrease in number of invading cells by light microscope. P values were calculated with ANOVA analysis of variance along with Bonferroni post test. The error bar represents standard deviation. Differences were considered as statistically significant when the P-value was less <0.005 (**) and <0.001 (***). Data are expressed as mean ± SD of triplicates.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "Oncogenic signaling", "Molecular cell biology", "Complementary and alternative medicine", "Gastroenterology and hepatology", "Gastrointestinal cancers", "oncology", "Cancers and neoplasms", "Gastrointestinal tumors", "cholangiocarcinoma", "Basic cancer research", "Cancer treatment", "cells", "capsaicin"], "article_id"=>1073116, "categories"=>["Biological Sciences"], "users"=>["Annika Wutka", "Vindhya Palagani", "Samarpita Barat", "Xi Chen", "Mona El Khatib", "Julian Götze", "Hanane Belahmer", "Steffen Zender", "Przemyslaw Bozko", "Nisar P. Malek", "Ruben R. Plentz"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095605.g004", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Invasion_activity_of_human_cholangiocarcinoma_cells_in_response_to_capsaicin_treatment_/1073116", "title"=>"Invasion activity of human cholangiocarcinoma cells in response to capsaicin treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-18 10:01:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/1553128"], "description"=>"<p>(A) SZ-1 and (B) TFK-1 cells were treated with control (DMSO) and capsaicin (150 µM, 200 µM) for 24 h, 48 h and 96 h. The expression of EMT markers: E-cadherin, N-cadherin and Vimentin were analyzed by Western blot. β-actin was used as a loading control. (A) SZ1 and (B) TFK1 cells showed an increase of epithelial marker E-cadherin and a dose- and time-dependent decrease of Vimentin. N-cadherin expression was nearly unchanged for both cholangiocarcinoma cell lines.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "Oncogenic signaling", "Molecular cell biology", "Complementary and alternative medicine", "Gastroenterology and hepatology", "Gastrointestinal cancers", "oncology", "Cancers and neoplasms", "Gastrointestinal tumors", "cholangiocarcinoma", "Basic cancer research", "Cancer treatment", "impairs", "epithelial", "mesenchymal"], "article_id"=>1073130, "categories"=>["Biological Sciences"], "users"=>["Annika Wutka", "Vindhya Palagani", "Samarpita Barat", "Xi Chen", "Mona El Khatib", "Julian Götze", "Hanane Belahmer", "Steffen Zender", "Przemyslaw Bozko", "Nisar P. Malek", "Ruben R. Plentz"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095605.g006", "stats"=>{"downloads"=>2, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Capsaicin_impairs_epithelial_mesenchymal_transition_/1073130", "title"=>"Capsaicin impairs epithelial mesenchymal transition.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-18 10:01:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/1553119"], "description"=>"<p>Soft agar assay was performed in capsaicin-treated human cholangiocarcinoma cells SZ-1 (A) and TFK-1 (B), with quantification. Compared to DMSO (control) capsaicin inhibits colony formation at a concentration starting at 150 µM. P values were calculated with ANOVA analysis of variance along with Bonferroni post test. The error bar represents standard deviation. Differences were considered as statistically significant when the P-value was less <0.05 (*), <0.005 (**) and <0.001 (***). Data are expressed as mean ± SD of triplicates.</p>", "links"=>[], "tags"=>["cell biology", "Signal transduction", "cell signaling", "Oncogenic signaling", "Molecular cell biology", "Complementary and alternative medicine", "Gastroenterology and hepatology", "Gastrointestinal cancers", "oncology", "Cancers and neoplasms", "Gastrointestinal tumors", "cholangiocarcinoma", "Basic cancer research", "Cancer treatment", "suppresses"], "article_id"=>1073121, "categories"=>["Biological Sciences"], "users"=>["Annika Wutka", "Vindhya Palagani", "Samarpita Barat", "Xi Chen", "Mona El Khatib", "Julian Götze", "Hanane Belahmer", "Steffen Zender", "Przemyslaw Bozko", "Nisar P. Malek", "Ruben R. Plentz"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095605.g005", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Capsaicin_treatment_suppresses_the_colony_formation_ability_of_cholangiocarcinoma_cells_/1073121", "title"=>"Capsaicin treatment suppresses the colony formation ability of cholangiocarcinoma cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-18 10:01:48"}

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