Identification and Characterisation of Heat Shock Protein 70 in Thermal Stressed Blastocystis sp
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{"title"=>"Identification and characterisation of heat shock protein 70 in thermal stressed Blastocystis sp.", "type"=>"journal", "authors"=>[{"first_name"=>"T.", "last_name"=>"Gaythri", "scopus_author_id"=>"56943356000"}, {"first_name"=>"K.", "last_name"=>"Suresh", "scopus_author_id"=>"7103241779"}, {"first_name"=>"B.", "last_name"=>"Subha", "scopus_author_id"=>"57118003200"}, {"first_name"=>"R.", "last_name"=>"Kalyani", "scopus_author_id"=>"56943194100"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"25180903", "doi"=>"10.1371/journal.pone.0095608", "issn"=>"19326203", "scopus"=>"2-s2.0-84946235623", "pui"=>"608259318", "sgr"=>"84946235623"}, "id"=>"7c59390d-3a35-358e-a2b4-b7a809f63f53", "abstract"=>"Protistan parasites in order to ensure their viability and demonstrate successful progression in their life cycle need to respond towards various environmental stressors. Blastocystis sp. is known to be the most commonly found intestinal protistan parasite in any human stool surveys and has been incriminated to be responsible for diarrhea and bloating stomach. The present study demonstrates for the first time the presence of HSP70 in subtypes of Blastocystis sp. when the cultures were subjected to temperature of 39 and 41°C where the growth of parasites was reduced to a minimum to majority being granular forms. The growth of parasites exposed to higher temperatures however doubled compared to the controls when the parasites were re-cultured back at 37°C. Upon thermal stress at 41°C, subtype 3 and subtype 5 isolates' growth reached up to 2.97×106 and 3.05×106 cells/ml compared to their respective controlled culture tubes at 37°C which peaked only at 1.34×106 and 1.70×106 cells/ml respectively. The designed primer set that amplified Blastocystis sp. subtype 7 HSP70 gene in subtypes 1, 3 and 5 was against a conserved region. The gene was amplified at 318 bp. The multiple sequence alignment showed that the targeted sequence length ranges from 291-295 bp. The pair wise alignment result showed that the sequence identity among the four sequence ranges from 88% to 96%. These findings were further evidenced by the up regulation of HSP70 gene in thermal stressed isolates of subtype 3 and 5 at 41°C. Higher number of granular forms was significantly found in thermal stressed isolates of subtype 3 and 5 which implicates that this life cycle stage has a role in responding to thermal stress.", "link"=>"http://www.mendeley.com/research/identification-characterisation-heat-shock-protein-70-thermal-stressed-blastocystis-sp", "reader_count"=>16, "reader_count_by_academic_status"=>{"Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Other"=>2, "Student > Master"=>3, "Student > Bachelor"=>5}, "reader_count_by_user_role"=>{"Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Other"=>2, "Student > Master"=>3, "Student > Bachelor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Medicine and Dentistry"=>4, "Agricultural and Biological Sciences"=>6, "Business, Management and Accounting"=>1, "Chemistry"=>1, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Chemistry"=>{"Chemistry"=>1}, "Social Sciences"=>{"Social Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"Germany"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1658346"], "description"=>"<p>Isolates of subtype 1, 3, and 5 were thermal stressed at 41°C for three different lengths of time. Values are presented in fold difference observed in comparison with control (37°C) and normalized against endogenous gene (ssu_rRNA). A positive fold difference refers to up regulation and vice versa. Student t-test was performed and a statistically significant difference was found between control and thermal stressed samples (**p<0.01, and ***p<0.001).</p>", "links"=>[], "tags"=>["Blastocystis sp", "subtype 7 HSP 70 gene", "HSP 70 gene", "life cycle stage", "life cycle need", "subtype 3", "sequence length ranges", "heat shock protein 70", "Blastocystis sp Protistan parasites"], "article_id"=>1158432, "categories"=>["Uncategorised"], "users"=>["T. Gaythri", "K. Suresh", "B. Subha", "R. Kalyani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095608.g007", "stats"=>{"downloads"=>0, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gene_expression_profile_of_HSP70_in_thermal_stressed_Blastocystis_sp_/1158432", "title"=>"Gene expression profile of HSP70 in thermal stressed <i>Blastocystis sp</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-02 03:57:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1658347"], "description"=>"<p>Biological illustration of thermal stressed <i>Blastocystis sp</i>.</p>", "links"=>[], "tags"=>["Blastocystis sp", "subtype 7 HSP 70 gene", "HSP 70 gene", "life cycle stage", "life cycle need", "subtype 3", "sequence length ranges", "heat shock protein 70", "Blastocystis sp Protistan parasites"], "article_id"=>1158433, "categories"=>["Uncategorised"], "users"=>["T. Gaythri", "K. Suresh", "B. Subha", "R. Kalyani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095608.g008", "stats"=>{"downloads"=>0, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Biological_illustration_of_thermal_stressed_Blastocystis_sp_/1158433", "title"=>"Biological illustration of thermal stressed <i>Blastocystis sp</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-02 03:57:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1658340"], "description"=>"<p>Controlled temperature (37°C) and thermal stress temperatures (39°C and 41°C) were used. (A) Subtype 1 did not show significant growth difference between control and thermal stressed isolates. (B) Subtype 3 and (C) Subtype 5 which thermal stressed at 41°C showed significantly higher growth compared to isolate at 37°C.</p>", "links"=>[], "tags"=>["Blastocystis sp", "subtype 7 HSP 70 gene", "HSP 70 gene", "life cycle stage", "life cycle need", "subtype 3", "sequence length ranges", "heat shock protein 70", "Blastocystis sp Protistan parasites"], "article_id"=>1158426, "categories"=>["Uncategorised"], "users"=>["T. Gaythri", "K. Suresh", "B. Subha", "R. Kalyani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095608.g001", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Growth_of_Blastocystis_sp_/1158426", "title"=>"Growth of <i>Blastocystis</i> sp.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-02 03:57:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1658341"], "description"=>"<p>Isolates of subtype 1, 3, and 5 were grown at controlled temperature (37°C) and thermal stressed temperature (41°C). (A) Vacuolar form was highly significant in controlled isolates compared to their respective stressed isolates of all the three subtypes. (B) Granular form was highly significant in the thermal stressed isolates of subtype 3 and 5.</p>", "links"=>[], "tags"=>["Blastocystis sp", "subtype 7 HSP 70 gene", "HSP 70 gene", "life cycle stage", "life cycle need", "subtype 3", "sequence length ranges", "heat shock protein 70", "Blastocystis sp Protistan parasites"], "article_id"=>1158427, "categories"=>["Uncategorised"], "users"=>["T. Gaythri", "K. Suresh", "B. Subha", "R. Kalyani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095608.g002", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Blastocystis_sp_forms_in_control_and_thermal_stressed_isolates_/1158427", "title"=>"<i>Blastocystis</i> sp. forms in control and thermal stressed isolates.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-02 03:57:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1658342"], "description"=>"<p>Image A and B is the control isolate at 37°C, while C and D is the thermal stressed isolate at 41°C. Note: The vacuolar forms in control isolates are stained dull green (Image B), and the granular form (arrow) in thermal stressed isolates are stained bright green (Image D).</p>", "links"=>[], "tags"=>["Blastocystis sp", "subtype 7 HSP 70 gene", "HSP 70 gene", "life cycle stage", "life cycle need", "subtype 3", "sequence length ranges", "heat shock protein 70", "Blastocystis sp Protistan parasites"], "article_id"=>1158428, "categories"=>["Uncategorised"], "users"=>["T. Gaythri", "K. Suresh", "B. Subha", "R. Kalyani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095608.g003", "stats"=>{"downloads"=>1, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Bright_field_and_epifluorescence_images_of_Blastocystis_sp_stained_with_acridine_orange_400_/1158428", "title"=>"Bright field and epifluorescence images of <i>Blastocystis</i> sp. stained with acridine orange (×400).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-02 03:57:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1658343"], "description"=>"<p>Localization of the target sequence in the <i>Blastocystis sp.</i> heat shock protein 70 (HSP70) gene for subtype 7.</p>", "links"=>[], "tags"=>["Blastocystis sp", "subtype 7 HSP 70 gene", "HSP 70 gene", "life cycle stage", "life cycle need", "subtype 3", "sequence length ranges", "heat shock protein 70", "Blastocystis sp Protistan parasites"], "article_id"=>1158429, "categories"=>["Uncategorised"], "users"=>["T. Gaythri", "K. Suresh", "B. Subha", "R. Kalyani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095608.g004", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Localization_of_the_target_sequence_in_the_Blastocystis_sp_heat_shock_protein_70_HSP70_gene_for_subtype_7_/1158429", "title"=>"Localization of the target sequence in the <i>Blastocystis sp.</i> heat shock protein 70 (HSP70) gene for subtype 7.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-02 03:57:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1658344"], "description"=>"<p>The amplicon size of the target region is 318 bp. Lane M = DNA markers of 100 bp plus DNA ladder (Fermentas, USA); lane 1–4 =  subtype 1; lane 5–7 =  subtype 3 and lane 8 =  subtype 5.</p>", "links"=>[], "tags"=>["Blastocystis sp", "subtype 7 HSP 70 gene", "HSP 70 gene", "life cycle stage", "life cycle need", "subtype 3", "sequence length ranges", "heat shock protein 70", "Blastocystis sp Protistan parasites"], "article_id"=>1158430, "categories"=>["Uncategorised"], "users"=>["T. Gaythri", "K. Suresh", "B. Subha", "R. Kalyani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095608.g005", "stats"=>{"downloads"=>3, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Agarose_gel_image_of_the_amplified_Blastocystis_sp_heat_shock_protein_HSP_70_gene_/1158430", "title"=>"Agarose gel image of the amplified <i>Blastocystis sp</i> heat shock protein (HSP) 70 gene.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-02 03:57:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1658345"], "description"=>"<p>Three random isolates of <i>Blastocystis sp</i>. was used. Primers (HSP70_F and HSP70_R) are indicated in bold and underlined.</p>", "links"=>[], "tags"=>["Blastocystis sp", "subtype 7 HSP 70 gene", "HSP 70 gene", "life cycle stage", "life cycle need", "subtype 3", "sequence length ranges", "heat shock protein 70", "Blastocystis sp Protistan parasites"], "article_id"=>1158431, "categories"=>["Uncategorised"], "users"=>["T. Gaythri", "K. Suresh", "B. Subha", "R. Kalyani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095608.g006", "stats"=>{"downloads"=>1, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ClustalW2_multiple_sequence_alignment_of_the_target_sequence_Blastocystis_sp_heat_shock_protein_70_/1158431", "title"=>"ClustalW2 multiple sequence alignment of the target sequence <i>Blastocystis sp.</i> heat shock protein 70.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-02 03:57:32"}

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