Estrogenic Regulation of Histamine Receptor Subtype H1 Expression in the Ventromedial Nucleus of the Hypothalamus in Female Rats
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{"title"=>"Estrogenic regulation of histamine receptor subtype H1 expression in the ventromedial nucleus of the hypothalamus in female rats", "type"=>"journal", "authors"=>[{"first_name"=>"Hiroko", "last_name"=>"Mori", "scopus_author_id"=>"37026817100"}, {"first_name"=>"Ken Ichi", "last_name"=>"Matsuda", "scopus_author_id"=>"35418472900"}, {"first_name"=>"Masanaga", "last_name"=>"Yamawaki", "scopus_author_id"=>"56159632500"}, {"first_name"=>"Mitsuhiro", "last_name"=>"Kawata", "scopus_author_id"=>"55342941200"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"24805361", "sgr"=>"84900555287", "doi"=>"10.1371/journal.pone.0096232", "scopus"=>"2-s2.0-84900555287", "pui"=>"373094140", "issn"=>"19326203"}, "id"=>"7a0f5337-dd89-390d-b451-6148a0156436", "abstract"=>"Female sexual behavior is controlled by central estrogenic action in the ventromedial nucleus of the hypothalamus (VMN). This region plays a pivotal role in facilitating sex-related behavior in response to estrogen stimulation via neural activation by several neurotransmitters, including histamine, which participates in this mechanism through its strong neural potentiating action. However, the mechanism through which estrogen signaling is linked to the histamine system in the VMN is unclear. This study was undertaken to investigate the relationship between estrogen and histamine receptor subtype H1 (H1R), which is a potent subtype among histamine receptors in the brain. We show localization of H1R exclusively in the ventrolateral subregion of the female VMN (vl VMN), and not in the dorsomedial subregion. In the vl VMN, abundantly expressed H1R were mostly colocalized with estrogen receptor α. Intriguingly, H1R mRNA levels in the vl VMN were significantly elevated in ovariectomized female rats treated with estrogen benzoate. These data suggest that estrogen can amplify histamine signaling by enhancing H1R expression in the vl VMN. This enhancement of histamine signaling might be functionally important for allowing neural excitation in response to estrogen stimulation of the neural circuit and may serve as an accelerator of female sexual arousal.", "link"=>"http://www.mendeley.com/research/estrogenic-regulation-histamine-receptor-subtype-h1-expression-ventromedial-nucleus-hypothalamus-fem", "reader_count"=>9, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>1, "Student > Master"=>3, "Other"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>1, "Student > Master"=>3, "Other"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Materials Science"=>1, "Medicine and Dentistry"=>4, "Agricultural and Biological Sciences"=>1, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Japan"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1489119"], "description"=>"<p>H1R protein expression levels were quantified for males (n = 5), OVX females (oil injected controls, n = 5), OVX females with EB (5 µg EB, 24 h or 48 h post-injection, n = 5 each), and OVX females with EB+ progesterone (5 µg EB, followed by 500 µg progesterone, n = 5). In the vl VMN, the H1R protein level tended to be increased by EB in OVX females (not statistically significant) and decreased by progesterone. In the dm VMN, hormonal manipulation did not affect H1R protein expression. Data are presented as the mean ±SEM. **, P<0.01. Data are expressed as the ratios of H1R protein to tubulin protein in the vl VMN and dm VMN regions. Since the immunoblotting were obtained independently for each region (from the respective immunoblotting membranes for the vl VMN and dm VMN), data were not to be compared between the two regions.</p>", "links"=>[], "tags"=>["agriculture", "Animal management", "anatomy", "nervous system", "neuroanatomy", "Endocrine system", "Biochemistry", "Neurochemistry", "Neuroendocrinology", "hormones", "cell biology", "Signal transduction", "cell signaling", "Membrane receptor signaling", "Hormone receptor signaling", "Molecular cell biology", "Computational biology", "genetics", "gene expression", "Molecular genetics", "neuroscience", "organisms", "animals", "vertebrates", "mammals", "rodents", "rats", "physiology", "Endocrine physiology", "Veterinary science", "Zoology", "Animal behavior", "Animal sexual behavior", "Model organisms", "Animal models", "normalized", "h1r", "vl", "vmn", "dm"], "article_id"=>1019806, "categories"=>["Biological Sciences"], "users"=>["Hiroko Mori", "Ken-Ichi Matsuda", "Masanaga Yamawaki", "Mitsuhiro Kawata"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0096232.g005", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Quantitative_evaluation_of_normalized_H1R_protein_expression_in_the_vl_VMN_and_dm_VMN_/1019806", "title"=>"Quantitative evaluation of normalized H1R protein expression in the vl VMN and dm VMN.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-07 04:01:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/1489110"], "description"=>"<p>(A–F) Confocal microscopy images of dual immunofluorescence labeling of ERα (green, A and D) and H1R (red, B and E) in adult female rats. ERα-ir neurons are abundant in the vl VMN, but few are present in the dm VMN (A). (B) The highest density of H1R expression was found in the vl VMN. Merged images showed the coexistence of abundant ERα-ir and H1R-ir in the VMN (C and F). (D–F) Medium magnification images of representative neural groups within the vl VMN (area with a dotted line), allowing identification of cellular localization of ERα and H1R. (G and H) Detection of H1R immunoreactivities in VMN with (H) or without (G) control peptide. Preabsorption of H1R antibody with its peptide eliminated H1R immunoreactivities (H). 3V, Third ventricle; VMN, ventromedial nucleus of the hypothalamus; vl, ventrolateral part of the VMN; dm, dorsomedial part of the VMN; Me, median eminence; ARC, arcuate nucleus. Scale bars, 500 µm (A–C), 50 µm (D–H).</p>", "links"=>[], "tags"=>["agriculture", "Animal management", "anatomy", "nervous system", "neuroanatomy", "Endocrine system", "Biochemistry", "Neurochemistry", "Neuroendocrinology", "hormones", "cell biology", "Signal transduction", "cell signaling", "Membrane receptor signaling", "Hormone receptor signaling", "Molecular cell biology", "Computational biology", "genetics", "gene expression", "Molecular genetics", "neuroscience", "organisms", "animals", "vertebrates", "mammals", "rodents", "rats", "physiology", "Endocrine physiology", "Veterinary science", "Zoology", "Animal behavior", "Animal sexual behavior", "Model organisms", "Animal models", "h1r"], "article_id"=>1019802, "categories"=>["Biological Sciences"], "users"=>["Hiroko Mori", "Ken-Ichi Matsuda", "Masanaga Yamawaki", "Mitsuhiro Kawata"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0096232.g002", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distribution_of_H1R_in_the_rat_VMN_/1019802", "title"=>"Distribution of H1R in the rat VMN.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-07 04:01:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/1489123", "https://ndownloader.figshare.com/files/1489124"], "description"=>"<div><p>Female sexual behavior is controlled by central estrogenic action in the ventromedial nucleus of the hypothalamus (VMN). This region plays a pivotal role in facilitating sex-related behavior in response to estrogen stimulation via neural activation by several neurotransmitters, including histamine, which participates in this mechanism through its strong neural potentiating action. However, the mechanism through which estrogen signaling is linked to the histamine system in the VMN is unclear. This study was undertaken to investigate the relationship between estrogen and histamine receptor subtype H1 (H1R), which is a potent subtype among histamine receptors in the brain. We show localization of H1R exclusively in the ventrolateral subregion of the female VMN (vl VMN), and not in the dorsomedial subregion. In the vl VMN, abundantly expressed H1R were mostly colocalized with estrogen receptor α. Intriguingly, H1R mRNA levels in the vl VMN were significantly elevated in ovariectomized female rats treated with estrogen benzoate. These data suggest that estrogen can amplify histamine signaling by enhancing H1R expression in the vl VMN. This enhancement of histamine signaling might be functionally important for allowing neural excitation in response to estrogen stimulation of the neural circuit and may serve as an accelerator of female sexual arousal.</p></div>", "links"=>[], "tags"=>["agriculture", "Animal management", "anatomy", "nervous system", "neuroanatomy", "Endocrine system", "Biochemistry", "Neurochemistry", "Neuroendocrinology", "hormones", "cell biology", "Signal transduction", "cell signaling", "Membrane receptor signaling", "Hormone receptor signaling", "Molecular cell biology", "Computational biology", "genetics", "gene expression", "Molecular genetics", "neuroscience", "organisms", "animals", "vertebrates", "mammals", "rodents", "rats", "physiology", "Endocrine physiology", "Veterinary science", "Zoology", "Animal behavior", "Animal sexual behavior", "Model organisms", "Animal models", "histamine", "receptor", "subtype", "h1", "ventromedial", "nucleus", "hypothalamus"], "article_id"=>1019810, "categories"=>["Biological Sciences"], "users"=>["Hiroko Mori", "Ken-Ichi Matsuda", "Masanaga Yamawaki", "Mitsuhiro Kawata"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0096232.s001", "https://dx.doi.org/10.1371/journal.pone.0096232.s002"], "stats"=>{"downloads"=>7, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Estrogenic_Regulation_of_Histamine_Receptor_Subtype_H1_Expression_in_the_Ventromedial_Nucleus_of_the_Hypothalamus_in_Female_Rats_/1019810", "title"=>"Estrogenic Regulation of Histamine Receptor Subtype H1 Expression in the Ventromedial Nucleus of the Hypothalamus in Female Rats", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-05-07 04:01:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/1489118"], "description"=>"<p>Normalized H1R mRNA expression levels were quantified for males (n = 5), OVX females (oil injected controls, n = 5), OVX females with EB (5 µg EB, 24 h or 48 h post-injection, n = 5 each), and OVX females with EB + progesterone (5 µg EB, followed by 500 µg progesterone, n = 5). In the vl VMN, H1R mRNA was significantly increased by EB in OVX females and decreased by progesterone. In the dm VMN, hormonal manipulation did not affect H1R mRNA expression. Data represents the absolute quantities of H1R mRNA compared with GAPDH mRNA amount. Data are presented as the mean ±SEM. **, P<0.01; *, P<0.05.</p>", "links"=>[], "tags"=>["agriculture", "Animal management", "anatomy", "nervous system", "neuroanatomy", "Endocrine system", "Biochemistry", "Neurochemistry", "Neuroendocrinology", "hormones", "cell biology", "Signal transduction", "cell signaling", "Membrane receptor signaling", "Hormone receptor signaling", "Molecular cell biology", "Computational biology", "genetics", "gene expression", "Molecular genetics", "neuroscience", "organisms", "animals", "vertebrates", "mammals", "rodents", "rats", "physiology", "Endocrine physiology", "Veterinary science", "Zoology", "Animal behavior", "Animal sexual behavior", "Model organisms", "Animal models", "normalized", "h1r", "mrna", "vl", "vmn", "dm"], "article_id"=>1019805, "categories"=>["Biological Sciences"], "users"=>["Hiroko Mori", "Ken-Ichi Matsuda", "Masanaga Yamawaki", "Mitsuhiro Kawata"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0096232.g004", "stats"=>{"downloads"=>0, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Quantitative_evaluation_of_normalized_H1R_mRNA_expression_in_the_vl_VMN_and_dm_VMN_/1019805", "title"=>"Quantitative evaluation of normalized H1R mRNA expression in the vl VMN and dm VMN.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-07 04:01:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/1489117"], "description"=>"<p>(A, B, C, D, E) Representative images of dual immunofluorescence labeling of ERα (green) and H1R (red), obtained by confocal microscopy. Higher magnification images of individual cells in panel A are shown in B (area with a dotted line), and C, D and E (arrowheads). C–E are confocal images showing that ERα is exclusively localized in nuclei (green) and excluded from nucleoli (dark spot), while H1R is localized in the cell membrane (red). Panel E is a merge of the images from panels C and D. (F, G, H) Visualization of the cellular localization of ERα-ir in VMN neurons. ERα immunostaining (green) were observed in the nucleus defined by PI staining (red). Panel H is the merged image for panel F and G. ERα; ERα immunoreactivities, PI; propidium iodide. Scale bars, 5 µm (A–E), 10 µm (F–H).</p>", "links"=>[], "tags"=>["agriculture", "Animal management", "anatomy", "nervous system", "neuroanatomy", "Endocrine system", "Biochemistry", "Neurochemistry", "Neuroendocrinology", "hormones", "cell biology", "Signal transduction", "cell signaling", "Membrane receptor signaling", "Hormone receptor signaling", "Molecular cell biology", "Computational biology", "genetics", "gene expression", "Molecular genetics", "neuroscience", "organisms", "animals", "vertebrates", "mammals", "rodents", "rats", "physiology", "Endocrine physiology", "Veterinary science", "Zoology", "Animal behavior", "Animal sexual behavior", "Model organisms", "Animal models", "localization", "h1r", "vl", "vmn"], "article_id"=>1019804, "categories"=>["Biological Sciences"], "users"=>["Hiroko Mori", "Ken-Ichi Matsuda", "Masanaga Yamawaki", "Mitsuhiro Kawata"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0096232.g003", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Subcellular_localization_of_ER_945_and_H1R_in_vl_VMN_neurons_/1019804", "title"=>"Subcellular localization of ERα and H1R in vl VMN neurons.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-07 04:01:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/1489108"], "description"=>"<p>Quantitative analysis of H1R mRNA and H1R protein in the two subregions of the VMN was performed after selective dissection of the vl VMN and dm VMN using a stainless steel needle. The dotted lines show the locations of the VMN and vl and dm subregions in the rat basal hypothalamus. (A) Representative photograph of a coronal brain section after sampling (left side). The vl VMN and dm VMN were selectively dissected with the needle (inner diameter 900 µm). An outline of the rat basal hypothalamus used for identification and selective dissection of the VMN subregions. 3V, Third ventricle; VMN, ventromedial nucleus of the hypothalamus; vl, ventrolateral part of the VMN; dm, dorsomedial part of the VMN; Me, Median eminence; ARC, arcuate nucleus. Scale bars, 500 µm.</p>", "links"=>[], "tags"=>["agriculture", "Animal management", "anatomy", "nervous system", "neuroanatomy", "Endocrine system", "Biochemistry", "Neurochemistry", "Neuroendocrinology", "hormones", "cell biology", "Signal transduction", "cell signaling", "Membrane receptor signaling", "Hormone receptor signaling", "Molecular cell biology", "Computational biology", "genetics", "gene expression", "Molecular genetics", "neuroscience", "organisms", "animals", "vertebrates", "mammals", "rodents", "rats", "physiology", "Endocrine physiology", "Veterinary science", "Zoology", "Animal behavior", "Animal sexual behavior", "Model organisms", "Animal models", "vmn", "subregion"], "article_id"=>1019801, "categories"=>["Biological Sciences"], "users"=>["Hiroko Mori", "Ken-Ichi Matsuda", "Masanaga Yamawaki", "Mitsuhiro Kawata"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0096232.g001", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sampling_of_VMN_subregion_tissues_/1019801", "title"=>"Sampling of VMN subregion tissues.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-07 04:01:10"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[267]}, {"subject_area"=>"/Biology and life sciences/Neuroscience", "average_usage"=>[289]}]}
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