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{"title"=>"A quantitative assessment method for Ascaris eggs on hands", "type"=>"journal", "authors"=>[{"first_name"=>"Aurelie", "last_name"=>"Jeandron", "scopus_author_id"=>"36450388700"}, {"first_name"=>"Jeroen H.J.", "last_name"=>"Ensink", "scopus_author_id"=>"18834952700"}, {"first_name"=>"Stig M.", "last_name"=>"Thamsborg", "scopus_author_id"=>"7006081224"}, {"first_name"=>"Anders", "last_name"=>"Dalsgaard", "scopus_author_id"=>"7006552258"}, {"first_name"=>"Mita E.", "last_name"=>"Sengupta", "scopus_author_id"=>"26635927100"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84900435002", "doi"=>"10.1371/journal.pone.0096731", "sgr"=>"84900435002", "isbn"=>"1932-6203", "pmid"=>"24802859", "issn"=>"19326203", "pui"=>"373074586"}, "id"=>"0a11642f-32e6-32d4-8673-e977dabaab07", "abstract"=>"The importance of hands in the transmission of soil transmitted helminths, especially Ascaris and Trichuris infections, is under-researched. This is partly because of the absence of a reliable method to quantify the number of eggs on hands. Therefore, the aim of this study was to develop a method to assess the number of Ascaris eggs on hands and determine the egg recovery rate of the method. Under laboratory conditions, hands were seeded with a known number of Ascaris eggs, air dried and washed in a plastic bag retaining the washing water, in order to determine recovery rates of eggs for four different detergents (cationic [benzethonium chloride 0.1% and cetylpyridinium chloride CPC 0.1%], anionic [7X 1% - quadrafos, glycol ether, and dioctyl sulfoccinate sodium salt] and non-ionic [Tween80 0.1% -polyethylene glycol sorbitan monooleate]) and two egg detection methods (McMaster technique and FLOTAC). A modified concentration McMaster technique showed the highest egg recovery rate from bags. Two of the four diluted detergents (benzethonium chloride 0.1% and 7X 1%) also showed a higher egg recovery rate and were then compared with de-ionized water for recovery of helminth eggs from hands. The highest recovery rate (95.6%) was achieved with a hand rinse performed with 7X 1%. Washing hands with de-ionized water resulted in an egg recovery rate of 82.7%. This washing method performed with a low concentration of detergent offers potential for quantitative investigation of contamination of hands with Ascaris eggs and of their role in human infection. Follow-up studies are needed that validate the hand washing method under field conditions, e.g. including people of different age, lower levels of contamination and various levels of hand cleanliness.", "link"=>"http://www.mendeley.com/research/quantitative-assessment-method-ascaris-eggs-hands", "reader_count"=>19, "reader_count_by_academic_status"=>{"Researcher"=>4, "Student > Ph. D. Student"=>2, "Student > Master"=>7, "Student > Bachelor"=>5, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Researcher"=>4, "Student > Ph. D. Student"=>2, "Student > Master"=>7, "Student > Bachelor"=>5, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Engineering"=>5, "Medicine and Dentistry"=>8, "Agricultural and Biological Sciences"=>3, "Veterinary Science and Veterinary Medicine"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>5}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>8}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Unspecified"=>{"Unspecified"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "group_count"=>0}

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• http://doi.org/10.1371/journal.pntd.0007020
• http://doi.org/10.3390/w11061212
• http://doi.org/10.1590/0103-8478cr20160837
• http://doi.org/10.1155/2017/7515409
• http://doi.org/10.1016/j.pt.2015.08.007
• http://doi.org/10.1016/j.onehlt.2019.100088
• http://doi.org/10.4269/ajtmh.17-0072
• http://doi.org/10.1093/trstmh/tru115
• http://doi.org/10.1016/j.actatropica.2015.07.001
• http://doi.org/10.1093/trstmh/trw044
• http://doi.org/10.1371/journal.pntd.0005522
• http://doi.org/10.1371/journal.pone.0242240

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• {"id"=>"467347542821576707", "text"=>"Quantitative assessment method for #ascaris eggs on hands: http://t.co/J3PzTHMTOH @PLOSONE v. @washplusinfo #handwashing #OpenAccess", "created_at"=>"2014-05-16T16:55:11Z", "user"=>"Jodi_Gentry", "user_name"=>"Jodi Gentry", "user_profile_image"=>"http://pbs.twimg.com/profile_images/378800000615219976/6461f992fdb616223abb7147a7d99c68_normal.jpeg"}
• {"id"=>"479921558124003328", "text"=>"A new method has been developed for assessing Ascaris eggs on hands http://t.co/ToevIbnMFB #PlosOne", "created_at"=>"2014-06-20T09:39:50Z", "user"=>"MitaSengupta", "user_name"=>"Mita Sengupta", "user_profile_image"=>"http://abs.twimg.com/sticky/default_profile_images/default_profile_5_normal.png"}
• {"id"=>"481029433558855680", "text"=>"Beautifully simple tool! Rinse those hands #Ascaris #NTD transmission @PLOS @MitaSengupta \nhttp://t.co/qDNkoGNtBu", "created_at"=>"2014-06-23T11:02:08Z", "user"=>"AKildemoes", "user_name"=>"Anna Kildemoes", "user_profile_image"=>"http://pbs.twimg.com/profile_images/475271645481824257/-klPLm5U_normal.jpeg"}
• {"id"=>"481144460727058432", "text"=>"RT @AKildemoes: Beautifully simple tool! Rinse those hands #Ascaris #NTD transmission @PLOS @MitaSengupta \nhttp://t.co/qDNkoGNtBu", "created_at"=>"2014-06-23T18:39:13Z", "user"=>"AnnaSofieStensg", "user_name"=>"AnnaSofie Stensgaard", "user_profile_image"=>"http://pbs.twimg.com/profile_images/458524660812873728/F6zvmZdG_normal.jpeg"}

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Relative Metric

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F1000Prime