Evaluation of a Novel Magneto-Optical Method for the Detection of Malaria Parasites
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Evaluation of a novel magneto-optical method for the detection of malaria parasites", "type"=>"journal", "authors"=>[{"first_name"=>"Ágnes", "last_name"=>"Orbán", "scopus_author_id"=>"55624107500"}, {"first_name"=>"Ádám", "last_name"=>"Butykai", "scopus_author_id"=>"55627766600"}, {"first_name"=>"András", "last_name"=>"Molnár", "scopus_author_id"=>"57196777732"}, {"first_name"=>"Zsófia", "last_name"=>"Pröhle", "scopus_author_id"=>"56176658600"}, {"first_name"=>"Gergö", "last_name"=>"Fülöp", "scopus_author_id"=>"7005566303"}, {"first_name"=>"Tivadar", "last_name"=>"Zelles", "scopus_author_id"=>"24349729700"}, {"first_name"=>"Wasan", "last_name"=>"Forsyth", "scopus_author_id"=>"56177005200"}, {"first_name"=>"Danika", "last_name"=>"Hill", "scopus_author_id"=>"55536778000"}, {"first_name"=>"Ivo", "last_name"=>"Müller", "scopus_author_id"=>"15768092000"}, {"first_name"=>"Louis", "last_name"=>"Schofield", "scopus_author_id"=>"7006728410"}, {"first_name"=>"Maria", "last_name"=>"Rebelo", "scopus_author_id"=>"57197280657"}, {"first_name"=>"Thomas", "last_name"=>"Hänscheid", "scopus_author_id"=>"7004266513"}, {"first_name"=>"Stephan", "last_name"=>"Karl", "scopus_author_id"=>"24177167100"}, {"first_name"=>"István", "last_name"=>"Kézsmárki", "scopus_author_id"=>"6603204121"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84901271610", "doi"=>"10.1371/journal.pone.0096981", "sgr"=>"84901271610", "pmid"=>"24824542", "issn"=>"19326203", "pui"=>"373143750"}, "id"=>"16ac2d9b-178a-3fa9-a786-7e0094d19fdd", "abstract"=>"Improving the efficiency of malaria diagnosis is one of the main goals of current malaria research. We have recently developed a magneto-optical (MO) method which allows high-sensitivity detection of malaria pigment (hemozoin crystals) in blood via the magnetically induced rotational motion of the hemozoin crystals. Here, we evaluate this MO technique for the detection of Plasmodium falciparum in infected erythrocytes using in-vitro parasite cultures covering the entire intraerythrocytic life cycle. Our novel method detected parasite densities as low as ∼ 40 parasites per microliter of blood (0.0008% parasitemia) at the ring stage and less than 10 parasites/µL (0.0002% parasitemia) in the case of the later stages. These limits of detection, corresponding to approximately 20 pg/µL of hemozoin produced by the parasites, exceed that of rapid diagnostic tests and compete with the threshold achievable by light microscopic observation of blood smears. The MO diagnosis requires no special training of the operator or specific reagents for parasite detection, except for an inexpensive lysis solution to release intracellular hemozoin. The devices can be designed to a portable format for clinical and in-field tests. Besides testing its diagnostic performance, we also applied the MO technique to investigate the change in hemozoin concentration during parasite maturation. Our preliminary data indicate that this method may offer an efficient tool to determine the amount of hemozoin produced by the different parasite stages in synchronized cultures. Hence, it could eventually be used for testing the susceptibility of parasites to antimalarial drugs.", "link"=>"http://www.mendeley.com/research/evaluation-novel-magnetooptical-method-detection-malaria-parasites-2", "reader_count"=>14, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>2, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>2, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>2, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>2, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>3}, "reader_count_by_subject_area"=>{"Engineering"=>5, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>1, "Physics and Astronomy"=>1, "Chemistry"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>5}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>2}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84901271610"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84901271610?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84901271610&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84901271610&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84901271610", "dc:identifier"=>"SCOPUS_ID:84901271610", "eid"=>"2-s2.0-84901271610", "dc:title"=>"Evaluation of a novel magneto-optical method for the detection of malaria parasites", "dc:creator"=>"Orbán A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"5", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-05-13", "prism:coverDisplayDate"=>"13 May 2014", "prism:doi"=>"10.1371/journal.pone.0096981", "citedby-count"=>"19", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Budapest University of Technology and Economics", "affiliation-city"=>"Budapest", "affiliation-country"=>"Hungary"}], "pubmed-id"=>"24824542", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e96981", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0096981", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"75", "xml_views"=>"11", "html_views"=>"416"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"30", "xml_views"=>"2", "html_views"=>"149"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"56", "xml_views"=>"1", "html_views"=>"122"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"73"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"29", "xml_views"=>"3", "html_views"=>"131"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"127"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"107"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"90"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"130"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"82"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"142"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"3", "html_views"=>"44"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"3", "html_views"=>"76"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"40"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"44"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"69"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"96"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"3", "html_views"=>"37"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"40"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"3", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"60"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"42"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"14"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1496128"], "description"=>"<p>Panel A: The <i>ring stage culture</i> contained early rings, late rings and some early trophozoites of the first generation after synchronization. The <i>schizont stage culture</i> was on the verge of the first and second life cycles where most of the schizont stages have already turned to early ring stages of the second generation following invasion. Therefore, the ring stage culture contained only the hemozoin present in the parasites up to the early trophozoite stage, while the schizont stage culture had the entire hemozoin content formed during one generation of parasites with the largest portion produced by schizonts. Panel B: Light microscopy images of Giemsa stained thin blood films containing infected red blood cells with parasites in different stages of maturity (taken from these two cultures). In both panels the labels ER, LR, ET, LT, ES and LS correspond to early-ring, late-ring, early-trophozoite, late-trophozoite, early-schizont and late-schizont stages, respectively.</p>", "links"=>[], "tags"=>["microbiology", "Protozoology", "organisms", "protozoans", "Parasitic protozoans", "Malarial parasites", "Plasmodium falciparum", "Plasmodium malariae", "Plasmodium vivax", "Infectious diseases", "Parasitic diseases", "malaria", "Tropical diseases", "parasite", "stages", "cultures"], "article_id"=>1025402, "categories"=>["Biological Sciences"], "users"=>["Ágnes Orbán", "Ádám Butykai", "András Molnár", "Zsófia Pröhle", "Gergö Fülöp", "Tivadar Zelles", "Wasan Forsyth", "Danika Hill", "Ivo Müller", "Louis Schofield", "Maria Rebelo", "Thomas Hänscheid", "Stephan Karl", "István Kézsmárki"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0096981.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distribution_of_parasite_life_cycle_stages_in_the_two_Plasmodium_falciparum_cultures_used_in_the_present_study_/1025402", "title"=>"Distribution of parasite life cycle stages in the two <i>Plasmodium falciparum</i> cultures used in the present study.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-13 03:42:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1496129"], "description"=>"<p>Panel A: Red and blue curves show the frequency dependent MO signal for samples from the ring and schizont stage cultures, respectively, with various levels of parasite density given in <i>µ</i>L<sup>−1</sup> units on the right of the respective curves. The green curves shows the signal from uninfected reference samples. Data plotted with triangles and diamonds are the residual signal from freshly hemolyzed uninfected blood and water, respectively. The frequency scale corresponds to the rotation speed of the magnetic field. Panel B: Red and blue squares in panel B are the MO signal values measured at 20 Hz – indicated by a vertical solid line in panel A – for the dilution series prepared from the original ring and schizont stage cultures, respectively. Solid and open squares correspond to the duplicate samples labeled as samples #1 and samples #2. Triangles indicate the results obtained by remeasuring samples #1 with 24 h delay. The solid lines following the trend of the MO signal at higher parasite densities for ring (red line) and schizont (blue line) samples are guides for the eye. For ring and schizont stage samples with parasite densities lower than 10 parasites/<i>µ</i>L and 1 parasites/<i>µ</i>L, respectively, the MO signal does not further decrease. The green horizontal line shows the residual MO signal of uninfected blood, which is the mean detection limit of our method. The 95% confidence levels of this mean detection limit for the ring and schizont stage samples are indicated by red and blue dashed lines, respectively. Correspondingly, for ring and schizont stage samples with parasite density higher than 40 parasites/<i>µ</i>L and 10 parasites/<i>µ</i>L, respectively, the diagnosis is positive with a confidence of at least 95%. The background signal for freshly hemolyzed uninfected blood and water are also shown by dark and light grey lines. All these horizontal indicators are also shown in panel A for reference. The upper horizontal scale shows the corresponding levels of parasitemia.</p>", "links"=>[], "tags"=>["microbiology", "Protozoology", "organisms", "protozoans", "Parasitic protozoans", "Malarial parasites", "Plasmodium falciparum", "Plasmodium malariae", "Plasmodium vivax", "Infectious diseases", "Parasitic diseases", "malaria", "Tropical diseases", "detection", "parasitemia", "synchronized"], "article_id"=>1025403, "categories"=>["Biological Sciences"], "users"=>["Ágnes Orbán", "Ádám Butykai", "András Molnár", "Zsófia Pröhle", "Gergö Fülöp", "Tivadar Zelles", "Wasan Forsyth", "Danika Hill", "Ivo Müller", "Louis Schofield", "Maria Rebelo", "Thomas Hänscheid", "Stephan Karl", "István Kézsmárki"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0096981.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Magneto_optical_MO_detection_of_parasitemia_in_synchronized_Plasmodium_falciparum_cultures_/1025403", "title"=>"Magneto-optical (MO) detection of parasitemia in synchronized <i>Plasmodium falciparum</i> cultures.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-13 03:42:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1496130"], "description"=>"<p>The transmission electron microscopy (TEM) image in panel A and scanning electron microscopy (SEM) images in panels B, C and D show hemozoin crystals extracted from the samples previously used for the MO measurements. The granular background of the SEM images comes from the gold coating of the glass substrate. Individual crystals in panels C and D are marked with asterisks. The length of the black bar at the bottom right corner of each panel is 500 nm. The typical length of the extracted hemozoin crystals ranges from 200 nm to 500 nm. For comparison, a TEM image of synthetic hemozoin crystals used in our previous MO study <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096981#pone.0096981-Butykai1\" target=\"_blank\">[15]</a> is shown in panel E. These synthetic crystals have more elongated shape with a typical length of 500–900 nm. Panels F and G display TEM images of intact infected erythrocytes in the schizont stage. Distinct components of the parasite and the erythrocyte can be observed including the erythrocyte membrane (EM), parasite membrane (PM), food vacuole membrane (FVM), merozoites (M), hemoglobin transport vesicles (HbTV), knobs (K) and hemozoin (Hz).</p>", "links"=>[], "tags"=>["microbiology", "Protozoology", "organisms", "protozoans", "Parasitic protozoans", "Malarial parasites", "Plasmodium falciparum", "Plasmodium malariae", "Plasmodium vivax", "Infectious diseases", "Parasitic diseases", "malaria", "Tropical diseases", "crystals", "observed", "electron", "microscopy", "in-situ"], "article_id"=>1025404, "categories"=>["Biological Sciences"], "users"=>["Ágnes Orbán", "Ádám Butykai", "András Molnár", "Zsófia Pröhle", "Gergö Fülöp", "Tivadar Zelles", "Wasan Forsyth", "Danika Hill", "Ivo Müller", "Louis Schofield", "Maria Rebelo", "Thomas Hänscheid", "Stephan Karl", "István Kézsmárki"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0096981.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Hemozoin_crystals_observed_by_electron_microscopy_in_situ_and_after_isolation_/1025404", "title"=>"Hemozoin crystals observed by electron microscopy in-situ and after isolation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-13 03:42:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1496131"], "description"=>"<p>The lower and upper values of the hemozoin content correspond to the lower and upper values of the conversion rates quoted above. Note that the cultures have different parasite densities. We also estimated the hemozoin concentration of the two cultures based on MO signal using the conversion factor c<sub>HZ</sub> = 1 ng/<i>µ</i>L →  = 1.4% between the hemozoin concentration and the low-frequency (∼1 Hz) MO signal previously determined for artificial hemozoin crystals suspended in blood <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096981#pone.0096981-Butykai1\" target=\"_blank\">[15]</a>.</p>", "links"=>[], "tags"=>["microbiology", "Protozoology", "organisms", "protozoans", "Parasitic protozoans", "Malarial parasites", "Plasmodium falciparum", "Plasmodium malariae", "Plasmodium vivax", "Infectious diseases", "Parasitic diseases", "malaria", "Tropical diseases", "hemozoin", "rates", "parasite", "stages", "parasites"], "article_id"=>1025405, "categories"=>["Biological Sciences"], "users"=>["Ágnes Orbán", "Ádám Butykai", "András Molnár", "Zsófia Pröhle", "Gergö Fülöp", "Tivadar Zelles", "Wasan Forsyth", "Danika Hill", "Ivo Müller", "Louis Schofield", "Maria Rebelo", "Thomas Hänscheid", "Stephan Karl", "István Kézsmárki"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0096981.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Using_the_hemozoin_conversion_rates_reported_in_the_literature_for_the_different_parasite_stages_rings_3_5_trophozoites_15_20_and_schizonts_50_70_20_22_29_30_and_the_stage_distribution_of_the_parasites_Fig_1_we_estimated_the_hemozoin_content_of_culture_A/1025405", "title"=>"Using the hemozoin conversion rates reported in the literature for the different parasite stages (rings: 3–5%, trophozoites: 15–20%, and schizonts: 50–70%) [20]–[22], [29], [30] and the stage distribution of the parasites (Fig. 1), we estimated the hemozoin content of culture A (ring stage culture) and culture B (schizont stage culture).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-05-13 03:42:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1496132"], "description"=>"<div><p>Improving the efficiency of malaria diagnosis is one of the main goals of current malaria research. We have recently developed a magneto-optical (MO) method which allows high-sensitivity detection of malaria pigment (hemozoin crystals) in blood via the magnetically induced rotational motion of the hemozoin crystals. Here, we evaluate this MO technique for the detection of <i>Plasmodium falciparum</i> in infected erythrocytes using in-vitro parasite cultures covering the entire intraerythrocytic life cycle. Our novel method detected parasite densities as low as ∼40 parasites per microliter of blood (0.0008% parasitemia) at the ring stage and less than 10 parasites/<i>µ</i>L (0.0002% parasitemia) in the case of the later stages. These limits of detection, corresponding to approximately 20 pg/<i>µ</i>L of hemozoin produced by the parasites, exceed that of rapid diagnostic tests and compete with the threshold achievable by light microscopic observation of blood smears. The MO diagnosis requires no special training of the operator or specific reagents for parasite detection, except for an inexpensive lysis solution to release intracellular hemozoin. The devices can be designed to a portable format for clinical and in-field tests. Besides testing its diagnostic performance, we also applied the MO technique to investigate the change in hemozoin concentration during parasite maturation. Our preliminary data indicate that this method may offer an efficient tool to determine the amount of hemozoin produced by the different parasite stages in synchronized cultures. Hence, it could eventually be used for testing the susceptibility of parasites to antimalarial drugs.</p></div>", "links"=>[], "tags"=>["microbiology", "Protozoology", "organisms", "protozoans", "Parasitic protozoans", "Malarial parasites", "Plasmodium falciparum", "Plasmodium malariae", "Plasmodium vivax", "Infectious diseases", "Parasitic diseases", "malaria", "Tropical diseases", "magneto-optical", "detection"], "article_id"=>1025406, "categories"=>["Biological Sciences"], "users"=>["Ágnes Orbán", "Ádám Butykai", "András Molnár", "Zsófia Pröhle", "Gergö Fülöp", "Tivadar Zelles", "Wasan Forsyth", "Danika Hill", "Ivo Müller", "Louis Schofield", "Maria Rebelo", "Thomas Hänscheid", "Stephan Karl", "István Kézsmárki"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0096981"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Evaluation_of_a_Novel_Magneto_Optical_Method_for_the_Detection_of_Malaria_Parasites_/1025406", "title"=>"Evaluation of a Novel Magneto-Optical Method for the Detection of Malaria Parasites", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-05-13 03:42:00"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"37", "full-text"=>"38", "pdf"=>"24", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"24", "full-text"=>"24", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"28", "full-text"=>"33", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"19", "full-text"=>"23", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"33", "full-text"=>"38", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"21", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"21", "full-text"=>"20", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"23", "full-text"=>"14", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"19", "full-text"=>"13", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"32", "full-text"=>"35", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"27", "full-text"=>"23", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[267]}, {"subject_area"=>"/Biology and life sciences/Parasitology", "average_usage"=>[398, 644]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[280]}, {"subject_area"=>"/Medicine and health sciences/Physiology", "average_usage"=>[278]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[271]}, {"subject_area"=>"/Physical sciences/Materials science", "average_usage"=>[259]}]}
Loading … Spinner
There are currently no alerts