Antioxidant Activity of Inulin and Its Role in the Prevention of Human Colonic Muscle Cell Impairment Induced by Lipopolysaccharide Mucosal Exposure
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{"title"=>"Antioxidant activity of inulin and its role in the prevention of human colonic muscle cell impairment induced by lipopolysaccharide mucosal exposure", "type"=>"journal", "authors"=>[{"first_name"=>"Valentina", "last_name"=>"Pasqualetti", "scopus_author_id"=>"56177549300"}, {"first_name"=>"Annamaria", "last_name"=>"Altomare", "scopus_author_id"=>"11539354000"}, {"first_name"=>"Michele Pier Luca", "last_name"=>"Guarino", "scopus_author_id"=>"35901989300"}, {"first_name"=>"Vittoria", "last_name"=>"Locato", "scopus_author_id"=>"14028622200"}, {"first_name"=>"Silvia", "last_name"=>"Cocca", "scopus_author_id"=>"7801625991"}, {"first_name"=>"Sara", "last_name"=>"Cimini", "scopus_author_id"=>"55780427400"}, {"first_name"=>"Rossella", "last_name"=>"Palma", "scopus_author_id"=>"57191913444"}, {"first_name"=>"Rossana", "last_name"=>"Alloni", "scopus_author_id"=>"6603137437"}, {"first_name"=>"Laura", "last_name"=>"De Gara", "scopus_author_id"=>"7003546232"}, {"first_name"=>"Michele", "last_name"=>"Cicala", "scopus_author_id"=>"6701721784"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84901367480", "doi"=>"10.1371/journal.pone.0098031", "pui"=>"373153350", "issn"=>"19326203", "pmid"=>"24837182", "scopus"=>"2-s2.0-84901367480"}, "id"=>"349ea5fc-cedc-38bf-b5ba-6818d1aa54d7", "abstract"=>"BACKGROUND Fructans, such as inulin, are dietary fibers which stimulate gastro-intestinal (GI) function acting as prebiotics. Lipopolysaccharide (LPS) impairs GI motility, through production of reactive oxygen species. The antioxidant activity of various fructans was tested and the protective effect of inulin on colonic smooth muscle cell (SMC) impairment, induced by exposure of human mucosa to LPS, was assessed in an ex vivo experimental model. METHODS The antioxidant capacity of fructans was measured in an in vitro system that simulates cooking and digestion processes. Human colonic mucosa and submucosa, obtained from disease-free margins of resected segments for cancer, were sealed between two chambers, with the mucosal side facing upwards with Krebs solution with or without purified LPS from a pathogenic strain of Escherichia coli (O111:B4) and inulin (Frutafit IQ), and the submucosal side facing downwards into Krebs solution. The solutions on the submucosal side were collected following mucosal exposure to Krebs in the absence (N-undernatant) or presence of LPS (LPS-undernatant) or LPS+inulin (LPS+INU-undernatant). Undernatants were tested for their antioxidant activity and the effects on SMCs contractility. Inulin protective effects on mucosa and submucosa layers were assessed measuring the protein oxidation level in the experimental conditions analyzed. RESULTS Antioxidant activity of inulin, which was significantly higher compared to simple sugars, remained unaltered despite cooking and digestion processes. Inulin protected the mucosal and submucosal layers against protein oxidation. Following exposure to LPS-undernatant, a significant decrease in maximal acetylcholine (Ach)-induced contraction was observed when compared to the contraction induced in cells incubated with the N-undernatant (4±1% vs 25±5% respectively, P<0.005) and this effect was completely prevented by pre-incubation of LPS with Inulin (35±5%). CONCLUSIONS Inulin protects the human colon mucosa from LPS-induced damage and this effect appears to be related to the protective effect of inulin against LPS-induced oxidative stress.", "link"=>"http://www.mendeley.com/research/antioxidant-activity-inulin-role-prevention-human-colonic-muscle-cell-impairment-induced-lipopolysac", "reader_count"=>34, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>1, "Student > Doctoral Student"=>6, "Researcher"=>4, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>8, "Other"=>2, "Student > Bachelor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>1, "Student > Doctoral Student"=>6, "Researcher"=>4, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>8, "Other"=>2, "Student > Bachelor"=>3}, "reader_count_by_subject_area"=>{"Engineering"=>3, "Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>4, "Nursing and Health Professions"=>1, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>3, "Chemistry"=>5}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>3}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Chemistry"=>{"Chemistry"=>5}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Colombia"=>1, "Brazil"=>1}, "group_count"=>1}

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Figshare

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PMC Usage Stats | Further Information

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  • {"unique-ip"=>"27", "full-text"=>"34", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"23", "full-text"=>"22", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"32", "full-text"=>"34", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[280]}]}
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