The Reference Transcriptome of the Adult Female Biting Midge (Culicoides sonorensis) and Differential Gene Expression Profiling during Teneral, Blood, and Sucrose Feeding Conditions
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{"title"=>"The reference transcriptome of the adult female biting midge (Culicoides sonorensis) and differential gene expression profiling during teneral, blood, and sucrose feeding conditions", "type"=>"journal", "authors"=>[{"first_name"=>"Dana", "last_name"=>"Nayduch", "scopus_author_id"=>"8362768700"}, {"first_name"=>"Matthew B.", "last_name"=>"Lee", "scopus_author_id"=>"56180300900"}, {"first_name"=>"Christopher A.", "last_name"=>"Saski", "scopus_author_id"=>"8906288400"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84901395269", "sgr"=>"84901395269", "pui"=>"373178753", "isbn"=>"1932-6203", "pmid"=>"24866149", "doi"=>"10.1371/journal.pone.0098123"}, "id"=>"d3d477fe-5b5e-38c2-bc4f-ca66392a5590", "abstract"=>"Unlike other important vectors such as mosquitoes and sandflies, genetic and genomic tools for Culicoides biting midges are lacking, despite the fact that they vector a large number of arboviruses and other pathogens impacting humans and domestic animals world-wide. In North America, female Culicoides sonorensis midges are important vectors of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV), orbiviruses that cause significant disease in livestock and wildlife. Libraries of tissue-specific transcripts expressed in response to feeding and oral orbivirus challenge in C. sonorensis have previously been reported, but extensive genome-wide expression profiling in the midge has not. Here, we successfully used deep sequencing technologies to construct the first adult female C. sonorensis reference transcriptome, and utilized genome-wide expression profiling to elucidate the genetic response to blood and sucrose feeding over time. The adult female midge unigene consists of 19,041 genes, of which less than 7% are differentially expressed during the course of a sucrose meal, while up to 52% of the genes respond significantly in blood-fed midges, indicating hematophagy induces complex physiological processes. Many genes that were differentially expressed during blood feeding were associated with digestion (e.g. proteases, lipases), hematophagy (e.g., salivary proteins), and vitellogenesis, revealing many major metabolic and biological factors underlying these critical processes. Additionally, key genes in the vitellogenesis pathway were identified, which provides the first glimpse into the molecular basis of anautogeny for C. sonorensis. This is the first extensive transcriptome for this genus, which will serve as a framework for future expression studies, RNAi, and provide a rich dataset contributing to the ultimate goal of informing a reference genome assembly and annotation. Moreover, this study will serve as a foundation for subsequent studies of genome-wide expression analyses during early orbivirus infection and dissecting the molecular mechanisms behind vector competence in midges.", "link"=>"http://www.mendeley.com/research/reference-transcriptome-adult-female-biting-midge-culicoides-sonorensis-differential-gene-expression", "reader_count"=>34, "reader_count_by_academic_status"=>{"Unspecified"=>4, "Professor > Associate Professor"=>3, "Librarian"=>1, "Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>2, "Other"=>1, "Student > Master"=>3, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>4, "Professor > Associate Professor"=>3, "Librarian"=>1, "Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>2, "Other"=>1, "Student > Master"=>3, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>24, "Arts and Humanities"=>1, "Business, Management and Accounting"=>1, "Earth and Planetary Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>24}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>4}, "Environmental Science"=>{"Environmental Science"=>1}, "Arts and Humanities"=>{"Arts and Humanities"=>1}}, "reader_count_by_country"=>{"United States"=>1, "Taiwan"=>1, "United Kingdom"=>1, "Spain"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1511165"], "description"=>"<p>Blue bars indicate the number of unigene sequences versus size that were present at the initial Trinity build. Red bars indicate the number and lengths of unigenes after ORF filtering, and green bars represent the final unigene build after clustering and manual inspection.</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "unigene"], "article_id"=>1037805, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.g001", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Culicoides_sonorensis_unigene_assembly_and_filtering_/1037805", "title"=>"<i>Culicoides sonorensis</i> unigene assembly and filtering.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511173"], "description"=>"<p>Early blood is 2, 6, 12(pooled) and late blood is 36 h post ingestion, with Log<sub>10</sub> FPKM values indicated in legend. Further description of these genes can be found in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098123#pone-0098123-t001\" target=\"_blank\">Table 1</a>, and detailed statistic and values of fold change can be found in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098123#pone-0098123-t002\" target=\"_blank\">Table 2</a>.</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "comparative", "vitellogenesis", "genes"], "article_id"=>1037813, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.g009", "stats"=>{"downloads"=>1, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Heatmap_showing_comparative_expression_of_Culicoides_sonorensis_vitellogenesis_target_genes_in_response_to_blood_feeding_/1037813", "title"=>"Heatmap showing comparative expression of <i>Culicoides sonorensis</i> vitellogenesis target genes in response to blood feeding.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511172"], "description"=>"<p>Numbers of genes classified within GO-slim categories that were upregulated (blue) or downregulated (red) in response to blood feeding are shown, including comparisons of (A) teneral versus an early blood meal (B) teneral versus an late blood meal, and (C) early blood meal versus late blood meal. Note difference in vertical scale between A, B and C.</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "feeding"], "article_id"=>1037812, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.g008", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcriptional_response_to_blood_feeding_in_female_Culicoides_sonorensis_/1037812", "title"=>"Transcriptional response to blood feeding in female <i>Culicoides sonorensis</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511169"], "description"=>"<p>Comparison of the genes that were unique and shared between: A. teneral, early sucrose meal (2, 6, 12 h pooled; ES), and late sucrose meal (36 h; LS), or B. teneral, early blood meal (2, 6, 12 h pooled; EB), and late blood meal (36 h; LB). Right (boxes): The number of genes with differential expression profiles (P≤0.01), and the percent of shared unigene (in parentheses).</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "profiles", "temporal"], "article_id"=>1037809, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.g005", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcriptome_profiles_and_temporal_expression_analysis_early_or_late_within_diet_sucrose_or_blood_in_female_Culicoides_sonorensis_/1037809", "title"=>"Transcriptome profiles and temporal expression analysis (early or late) within diet (sucrose or blood) in female <i>Culicoides sonorensis</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511167"], "description"=>"<p>Classification and functional distrubuion of the 19,041 unigenes of the <i>C. sonorensis</i> transcriptome according to the 3 major classifications of the Gene Ontology: Biological Process, Molecular Function, and Cellular Component.</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "classification", "midge", "unigene"], "article_id"=>1037807, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.g003", "stats"=>{"downloads"=>1, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_GO_classification_of_the_adult_female_midge_unigene_set_/1037807", "title"=>"GO classification of the adult female midge unigene set.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511168"], "description"=>"<p>The volcano plot is composed of comparisons of replicate normalized transcriptional states between feeding conditions including teneral, early (2, 6, 12 h pooled) or late (36 h) blood or sucrose feeding. Log2 fold change for each unigene (dots) is shown; red dots indicate statistical significance (P≤0.01) of the pairwise comparison of net transcript abundance (up or down to right or left of midline, respectively).</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "pairwise", "comparisons", "illustrating", "differential"], "article_id"=>1037808, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.g004", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Volcano_plots_of_pairwise_comparisons_illustrating_diet_effects_on_differential_gene_expression_in_C_sonorensis_/1037808", "title"=>"Volcano plots of pairwise comparisons illustrating diet effects on differential gene expression in <i>C. sonorensis</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511166"], "description"=>"<p>The number of translated <i>Culicoides sonorensis</i> unigene alignments that have matches to the databases are shown. Unique numbers show how many <i>Culicoides</i> matches were unique to each of the databases, and the unmapped genes had not homology matches in any of the databases.</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "blastx", "alignment", "nr"], "article_id"=>1037806, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.g002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Unigene_BLASTx_alignment_to_the_nr_protein_database_and_Aedes_or_Culex_gene_set_/1037806", "title"=>"Unigene BLASTx alignment to the nr protein database, and <i>Aedes</i> or <i>Culex</i> gene set.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511177", "https://ndownloader.figshare.com/files/1511178", "https://ndownloader.figshare.com/files/1511179"], "description"=>"<div><p>Unlike other important vectors such as mosquitoes and sandflies, genetic and genomic tools for <i>Culicoides</i> biting midges are lacking, despite the fact that they vector a large number of arboviruses and other pathogens impacting humans and domestic animals world-wide. In North America, female <i>Culicoides sonorensis</i> midges are important vectors of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV), orbiviruses that cause significant disease in livestock and wildlife. Libraries of tissue-specific transcripts expressed in response to feeding and oral orbivirus challenge in <i>C. sonorensis</i> have previously been reported, but extensive genome-wide expression profiling in the midge has not. Here, we successfully used deep sequencing technologies to construct the first adult female <i>C. sonorensis</i> reference transcriptome, and utilized genome-wide expression profiling to elucidate the genetic response to blood and sucrose feeding over time. The adult female midge unigene consists of 19,041 genes, of which less than 7% are differentially expressed during the course of a sucrose meal, while up to 52% of the genes respond significantly in blood-fed midges, indicating hematophagy induces complex physiological processes. Many genes that were differentially expressed during blood feeding were associated with digestion (e.g. proteases, lipases), hematophagy (e.g., salivary proteins), and vitellogenesis, revealing many major metabolic and biological factors underlying these critical processes. Additionally, key genes in the vitellogenesis pathway were identified, which provides the first glimpse into the molecular basis of anautogeny for <i>C. sonorensis</i>. This is the first extensive transcriptome for this genus, which will serve as a framework for future expression studies, RNAi, and provide a rich dataset contributing to the ultimate goal of informing a reference genome assembly and annotation. Moreover, this study will serve as a foundation for subsequent studies of genome-wide expression analyses during early orbivirus infection and dissecting the molecular mechanisms behind vector competence in midges.</p></div>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "transcriptome", "biting", "midge", "differential", "profiling", "sucrose", "feeding"], "article_id"=>1037817, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0098123.s001", "https://dx.doi.org/10.1371/journal.pone.0098123.s002", "https://dx.doi.org/10.1371/journal.pone.0098123.s003"], "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/The_Reference_Transcriptome_of_the_Adult_Female_Biting_Midge_Culicoides_sonorensis_and_Differential_Gene_Expression_Profiling_during_Teneral_Blood_and_Sucrose_Feeding_Conditions/1037817", "title"=>"The Reference Transcriptome of the Adult Female Biting Midge (<i>Culicoides sonorensis</i>) and Differential Gene Expression Profiling during Teneral, Blood, and Sucrose Feeding Conditions", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511175"], "description"=>"<p>Ten (teneral), BE (early blood-fed), BL (late blood-fed) transcriptomes.</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "differential", "vitellogenesis", "genes", "blood-fed"], "article_id"=>1037815, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.t002", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcriptome_level_differential_expression_of_vitellogenesis_genes_in_blood_fed_female_C_sonorensis_/1037815", "title"=>"Transcriptome-level differential expression of vitellogenesis genes in blood-fed female C. sonorensis.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511176"], "description"=>"<p>Genetic components of vitellogenesis in <i>C. sonorensis</i>.</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "components", "vitellogenesis"], "article_id"=>1037816, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.t001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genetic_components_of_vitellogenesis_in_C_sonorensis_/1037816", "title"=>"Genetic components of vitellogenesis in <i>C. sonorensis</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511174"], "description"=>"<p>Consensus tree for full protein sequence of vitellogenins is shown for <i>Culicoides sonorensis</i> (including unigene number) and mosquitoes (including Genbank accession numbers). The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches Evolutionary analyses were conducted in Mega5 (<a href=\"http://www.megasoftware.net\" target=\"_blank\">www.megasoftware.net</a>). ClustalW alignment used for generating this tree is available in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098123#pone.0098123.s002\" target=\"_blank\">Fig. S2</a>.</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "mosquito"], "article_id"=>1037814, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.g010", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Maximum_likelihood_tree_for_Culicoides_sonorensis_and_mosquito_vitellogenins_/1037814", "title"=>"Maximum likelihood tree for <i>Culicoides sonorensis</i> and mosquito vitellogenins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511171"], "description"=>"<p>Global transcript upregulation (blue) and downregulation (red) in response to a blood meal (A; early and late transcriptomes combined) or sucrose meal (B; early and late transcriptomes combined). Note difference in vertical scale between A and B.</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "sucrose", "meals"], "article_id"=>1037811, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.g007", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Global_response_to_blood_and_sucrose_meals_in_female_Culicoides_sonorensis_/1037811", "title"=>"Global response to blood and sucrose meals in female <i>Culicoides sonorensis</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-27 02:56:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/1511170"], "description"=>"<p>Comparison of the genes that were unique and shared between: A. teneral and early blood (EB) and sucrose (ES) meals (both 2, 6, 12 h post ingestion, pooled), or B. teneral and late blood (LB) and sucrose (LS) meals (both 36 h). Right (boxes): The number of genes with differential expression profiles (P≤0.01), and the percent of shared unigene (in parentheses).</p>", "links"=>[], "tags"=>["agriculture", "pests", "Insect pests", "Computational biology", "genome analysis", "Transcriptome analysis", "genetics", "genomics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "culicoides", "epidemiology", "disease vectors", "Arthropod vectors", "Insect vectors", "Vector biology", "profiles", "temporal"], "article_id"=>1037810, "categories"=>["Biological Sciences"], "users"=>["Dana Nayduch", "Matthew B. Lee", "Christopher A. Saski"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098123.g006", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcriptome_profiles_and_temporal_expression_analysis_early_or_late_across_diet_sucrose_or_blood_in_female_Culicoides_sonorensis_/1037810", "title"=>"Transcriptome profiles and temporal expression analysis (early or late) across diet (sucrose or blood) in female <i>Culicoides sonorensis</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-27 02:56:46"}

PMC Usage Stats | Further Information

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Relative Metric

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