Aluminium Induced Endoplasmic Reticulum Stress Mediated Cell Death in SH-SY5Y Neuroblastoma Cell Line Is Independent of p53
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{"title"=>"Aluminium induced endoplasmic reticulum stress mediated cell death in SH-SY5Y neuroblastoma cell line is independent of p53", "type"=>"journal", "authors"=>[{"first_name"=>"Syed Husain Mustafa", "last_name"=>"Rizvi", "scopus_author_id"=>"57197392036"}, {"first_name"=>"Arshiya", "last_name"=>"Parveen", "scopus_author_id"=>"56198425700"}, {"first_name"=>"Anoop K.", "last_name"=>"Verma", "scopus_author_id"=>"57199779536"}, {"first_name"=>"Iqbal", "last_name"=>"Ahmad", "scopus_author_id"=>"57189764038"}, {"first_name"=>"Md", "last_name"=>"Arshad", "scopus_author_id"=>"56492324000"}, {"first_name"=>"Abbas Ali", "last_name"=>"Mahdi", "scopus_author_id"=>"7005124671"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84902322568", "isbn"=>"1932-6203", "pui"=>"373302227", "issn"=>"19326203", "scopus"=>"2-s2.0-84902322568", "doi"=>"10.1371/journal.pone.0098409"}, "id"=>"bc6bbead-2064-3344-990a-0ce4ea6d7989", "link"=>"http://www.mendeley.com/research/aluminium-induced-endoplasmic-reticulum-stress-mediated-cell-death-shsy5y-neuroblastoma-cell-line-in-1", "reader_count"=>1, "reader_count_by_academic_status"=>{"Researcher"=>1}, "reader_count_by_user_role"=>{"Researcher"=>1}, "reader_count_by_subject_area"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "reader_count_by_subdiscipline"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1514901"], "description"=>"<p>Phase-contrast image (20X objective) of the monolayers showing Al(mal)<sub>3</sub> induced cell loss and morphological changes in neuroblastoma cells at different concentration of Al(mal)<sub>3</sub> in cell culture plates.</p>", "links"=>[], "tags"=>["Biochemistry", "Neurochemistry", "cell biology", "Cell processes", "Cell death", "Cellular stress responses", "Molecular cell biology", "developmental biology", "Organism development", "aging", "Evolutionary biology", "toxicology", "Neurotoxicology", "Toxic agents", "health care", "Environmental health", "Mental health and psychiatry", "dementia", "Alzheimer disease", "neurology", "Neurodegenerative diseases", "Public and occupational health", "Occupational and industrial medicine", "induced", "morphological", "neuroblastoma"], "article_id"=>1040884, "categories"=>["Biological Sciences"], "users"=>["Syed Husain Mustafa Rizvi", "Arshiya Parveen", "Anoop K. Verma", "Iqbal Ahmad", "Md Arshad", "Abbas Ali Mahdi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098409.g001", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Al_mal_3_induced_morphological_changes_in_neuroblastoma_cells_/1040884", "title"=>"Al(mal)<sub>3</sub> induced morphological changes in neuroblastoma cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-30 03:46:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1514902"], "description"=>"<p>Viability of neuroblastoma cells (10,000 cells/well) was evaluated by MTT assay in 96-well plates at 24 h and 48 h exposure to (A) concentrations of Al(mal)<sub>3</sub> varying between 100 µM–600 µM and (B) Maltol alone at 3x concentration corresponding to each of Al(mal)<sub>3</sub> concentration. (C) Live, Apoptotic and necrotic populations estimated using LDH activity-based cytotoxicity assay and (D) Fluorescence microscopy of Annexin V/PI staining of neuroblastoma cells 24 h post Al(mal)<sub>3</sub> exposure (100 µM–600 µM) under 20X objective. The data are represented as means ±SE of three independent experiments. *P<0.05 vs. control.</p>", "links"=>[], "tags"=>["Biochemistry", "Neurochemistry", "cell biology", "Cell processes", "Cell death", "Cellular stress responses", "Molecular cell biology", "developmental biology", "Organism development", "aging", "Evolutionary biology", "toxicology", "Neurotoxicology", "Toxic agents", "health care", "Environmental health", "Mental health and psychiatry", "dementia", "Alzheimer disease", "neurology", "Neurodegenerative diseases", "Public and occupational health", "Occupational and industrial medicine", "induced", "apoptotic", "necrotic"], "article_id"=>1040885, "categories"=>["Biological Sciences"], "users"=>["Syed Husain Mustafa Rizvi", "Arshiya Parveen", "Anoop K. Verma", "Iqbal Ahmad", "Md Arshad", "Abbas Ali Mahdi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098409.g002", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Al_mal_3_induced_both_apoptotic_and_necrotic_cell_death_/1040885", "title"=>"Al(mal)<sub>3</sub> induced both apoptotic and necrotic cell death.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-30 03:46:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1514903"], "description"=>"<p>(A) ROS generation was assessed in terms of relative fluorescence units using 10 µM DCFH-DA in neuroblastoma cells after 1 h, 3 h and 6 h exposure to Al(mal)<sub>3</sub> in black-bottomed 96-well plates. (B) Fluorescence micrographs of ROS generation at (100 µM–600 µM) Al(mal)<sub>3</sub> concentrations in neuroblastoma cells obtained at 20X objective after 1 h treatment. (C) GSH levels were assessed in terms of relative fluorescence units using 5 µM CMFDA dye at 1 h, 3 h, 6 h and 24 h of Al(mal)<sub>3</sub> exposure. After conjugation with GSH, CMFDA is hydrolyzed to the fluorescent 5-chloromethyl-fluorescein (CMF) by cellular esterases. (D) Western blot analysis of Nrf2, NQO1 and pAKT at 24 h of Al(mal)<sub>3</sub> treatment (100 µM–600 µM). Band intensities were calculated by densitometry and change in protein expression (Al-treated) was calculated with respect to controls and expressed as fold change in graph. Results were normalized to <i>β</i>-actin. The data are represented as means ±SE of three independent experiments. *P<0.05 vs. control.</p>", "links"=>[], "tags"=>["Biochemistry", "Neurochemistry", "cell biology", "Cell processes", "Cell death", "Cellular stress responses", "Molecular cell biology", "developmental biology", "Organism development", "aging", "Evolutionary biology", "toxicology", "Neurotoxicology", "Toxic agents", "health care", "Environmental health", "Mental health and psychiatry", "dementia", "Alzheimer disease", "neurology", "Neurodegenerative diseases", "Public and occupational health", "Occupational and industrial medicine", "compromised", "antioxidant", "defenses", "neuroblastoma"], "article_id"=>1040886, "categories"=>["Biological Sciences"], "users"=>["Syed Husain Mustafa Rizvi", "Arshiya Parveen", "Anoop K. Verma", "Iqbal Ahmad", "Md Arshad", "Abbas Ali Mahdi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098409.g003", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Al_mal_3_compromised_antioxidant_defenses_of_neuroblastoma_cells_/1040886", "title"=>"Al(mal)<sub>3</sub> compromised antioxidant defenses of neuroblastoma cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-30 03:46:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1514904"], "description"=>"<p>(A) Ratio of Bax and Bcl<sub>2</sub> was determined using western blot analysis of protein obtained after 24 h exposure to Al(mal)<sub>3</sub> (100 µM–600 µM). Band intensities were calculated by densitometry and change in protein expression (Al-treated) was calculated with respect to controls and expressed as fold change in graph. Results were normalized to <i>β</i>-actin and then the ratio was obtained. (B) Relative quantification (RQ) of mRNA expression of Bax and Bcl<sub>2</sub> were determined by real time PCR, endogenous control used was GAPDH. (C) Caspase 9 and Caspase 3 activity was assessed in cell lysates of the SH-SY5Y following treatment with Al(mal)<sub>3</sub> (100 µM–600 µM) by spectrophotometric detection of the chromophore p-nitroaniline (pNA) formed after cleavage from the labeled substrate LEHD-pNA and DEVD-pNA using microtiter plate reader at 400 nm or 405 nm. (D) Western blot analysis of cytochrome c (Cyt c) in cytosolic fraction of Al(mal)<sub>3</sub> treated SH-SY5Y neuroblastoma cells at 24 h, results were normalized to <i>β</i>-actin. The data are represented as means ±SE of three independent experiments. *P<0.05 vs. control.</p>", "links"=>[], "tags"=>["Biochemistry", "Neurochemistry", "cell biology", "Cell processes", "Cell death", "Cellular stress responses", "Molecular cell biology", "developmental biology", "Organism development", "aging", "Evolutionary biology", "toxicology", "Neurotoxicology", "Toxic agents", "health care", "Environmental health", "Mental health and psychiatry", "dementia", "Alzheimer disease", "neurology", "Neurodegenerative diseases", "Public and occupational health", "Occupational and industrial medicine", "altered", "apoptotic"], "article_id"=>1040887, "categories"=>["Biological Sciences"], "users"=>["Syed Husain Mustafa Rizvi", "Arshiya Parveen", "Anoop K. Verma", "Iqbal Ahmad", "Md Arshad", "Abbas Ali Mahdi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098409.g004", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Al_mal_3_altered_the_levels_of_key_apoptotic_markers_/1040887", "title"=>"Al(mal)<sub>3</sub> altered the levels of key apoptotic markers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-30 03:46:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1514905"], "description"=>"<p>(A) Intracellular calcium levels were determined in terms of arbitrary fluorescence units using 5 µM calcium green-1 AM dye at time periods ranging from 30 mins to 300 mins of Al(mal)<sub>3</sub> exposure (100 µM–600 µM). Upon binding calcium ions, an increase in fluorescence of the respective dye is observed. (B) Relative quantification (RQ) of CHOP mRNA was done by Real time PCR following Al(mal)<sub>3</sub> treatment (100 µM–600 µM) for 24 h. GAPDH was used as endogenous control. (C) Western blot analysis of CHOP and caspase 12 at 24 h of Al(mal)<sub>3</sub> treatment (100 µM–600 µM). Band intensities were calculated by densitometry and change in protein expression (Al-treated) was calculated with respect to controls and expressed as fold change in graph. Results were normalized to <i>β</i>-actin. (D) Western blot analysis of Aβ(1–40) following 24 h of Al(mal)<sub>3</sub> treatment (100 µM–600 µM) showed oligomers formation at different concentrations of Al(mal)<sub>3</sub>. (E) Enzymatic activity of caspase 12 was assessed in cell lysates of the SH-SY5Y following treatment with Al(mal)<sub>3</sub> (100 µM–600 µM) by the detection of cleavage of substrate ATAD-AFC using fluorimeter (Ex/Em 400/505). The data are represented as means ±SE of three independent experiments.*P<0.05 vs. control;<sup> #</sup>P<0.05 vs. Al(mal)<sub>3</sub> treatment.</p>", "links"=>[], "tags"=>["Biochemistry", "Neurochemistry", "cell biology", "Cell processes", "Cell death", "Cellular stress responses", "Molecular cell biology", "developmental biology", "Organism development", "aging", "Evolutionary biology", "toxicology", "Neurotoxicology", "Toxic agents", "health care", "Environmental health", "Mental health and psychiatry", "dementia", "Alzheimer disease", "neurology", "Neurodegenerative diseases", "Public and occupational health", "Occupational and industrial medicine", "induced", "er", "neuroblastoma"], "article_id"=>1040888, "categories"=>["Biological Sciences"], "users"=>["Syed Husain Mustafa Rizvi", "Arshiya Parveen", "Anoop K. Verma", "Iqbal Ahmad", "Md Arshad", "Abbas Ali Mahdi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098409.g005", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Al_mal_3_induced_ER_stress_in_neuroblastoma_cells_/1040888", "title"=>"Al(mal)<sub>3</sub> induced ER stress in neuroblastoma cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-30 03:46:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1514906"], "description"=>"<p>(A) Western blot analysis of p53 and p21 at 24 h of Al(mal)<sub>3</sub> treatment (100 µM–600 µM). Band intensities were calculated by densitometry and change in protein expression (Al(mal)<sub>3</sub> treated) was calculated with respect to controls and expressed as fold change in graph. Results were normalized to <i>β</i>-actin. (B) Status of p53 ubiquitination at different concentrations of Al(mal)<sub>3</sub> (100 µM–600 µM) at 24 h. Total cell lysates were immunoprecipitated with anti-ubiquitin antibody followed by immunoblotting with anti-p53 antibody, p53-null cell line HL60 were used as negative control. (C) Relative quantification (RQ) of p53 mRNA expression was determined by real time PCR following Al(mal)<sub>3</sub> treatment (100 µM–600 µM) at 24 h. GAPDH was used as endogenous control. The data are represented as means ±SE of three independent experiments. *P<0.05 vs. control.</p>", "links"=>[], "tags"=>["Biochemistry", "Neurochemistry", "cell biology", "Cell processes", "Cell death", "Cellular stress responses", "Molecular cell biology", "developmental biology", "Organism development", "aging", "Evolutionary biology", "toxicology", "Neurotoxicology", "Toxic agents", "health care", "Environmental health", "Mental health and psychiatry", "dementia", "Alzheimer disease", "neurology", "Neurodegenerative diseases", "Public and occupational health", "Occupational and industrial medicine", "induced", "apoptosis", "p53"], "article_id"=>1040889, "categories"=>["Biological Sciences"], "users"=>["Syed Husain Mustafa Rizvi", "Arshiya Parveen", "Anoop K. Verma", "Iqbal Ahmad", "Md Arshad", "Abbas Ali Mahdi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098409.g006", "stats"=>{"downloads"=>5, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Al_mal_3_induced_apoptosis_in_p53_independent_manner_/1040889", "title"=>"Al(mal)<sub>3</sub> induced apoptosis in p53 independent manner.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-30 03:46:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1514907"], "description"=>"<p>SH-SY5Y cells were incubated with 400 µM Al(mal)<sub>3</sub> or with 400 µM Al(mal)<sub>3</sub> and peptide inhibitors of pan caspases (QVD-oph), caspase 12 (Z-ATAD), caspase 9 (Z-LEHD) and caspase 3 (Z-DEVD) for 24 h. Cell viability was determined by MTT reduction (A) and live, apoptotic and necrotic cell populations were assessed by estimation of LDH released (B) as per the protocol mentioned under <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098409#s2\" target=\"_blank\">Materials and Methods</a>. The data are represented as means ±SE of three independent experiments. *P<0.05 vs. control;<sup> #</sup>P<0.05 vs. Al(mal)<sub>3</sub> treatment.</p>", "links"=>[], "tags"=>["Biochemistry", "Neurochemistry", "cell biology", "Cell processes", "Cell death", "Cellular stress responses", "Molecular cell biology", "developmental biology", "Organism development", "aging", "Evolutionary biology", "toxicology", "Neurotoxicology", "Toxic agents", "health care", "Environmental health", "Mental health and psychiatry", "dementia", "Alzheimer disease", "neurology", "Neurodegenerative diseases", "Public and occupational health", "Occupational and industrial medicine", "caspase", "inhibitor", "12", "rescues", "sh-sy5y", "cells", "al"], "article_id"=>1040890, "categories"=>["Biological Sciences"], "users"=>["Syed Husain Mustafa Rizvi", "Arshiya Parveen", "Anoop K. Verma", "Iqbal Ahmad", "Md Arshad", "Abbas Ali Mahdi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098409.g007", "stats"=>{"downloads"=>2, "page_views"=>97, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Treatment_with_pan_caspase_inhibitor_and_caspase_12_inhibitor_rescues_SH_SY5Y_cells_from_Al_toxicity_/1040890", "title"=>"Treatment with pan caspase inhibitor and caspase 12 inhibitor rescues SH-SY5Y cells from Al toxicity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-30 03:46:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1514908"], "description"=>"<p>Al induces oxidative stress which perturbs the endoplasmic reticulum resulting into the release of calcium and ER stress related proteins like CHOP and Caspase 12. The altered cellular calcium levels disturb mitochondrial membrane permeability and induce cytochrome c release which activates caspase cascade. ER specific caspase 12 is also implicated in the activation of apoptosis through direct activation of caspase 3 or by caspase 9. Besides this, CHOP also disturbs the ratio of Bax/Bcl<sub>2</sub>. ER stress may be implicated in the inactivation of p53 in response to Al(mal)<sub>3</sub> toxicity or other unknown possible mechanism may be responsible for suppressing transcript levels of p53.</p>", "links"=>[], "tags"=>["Biochemistry", "Neurochemistry", "cell biology", "Cell processes", "Cell death", "Cellular stress responses", "Molecular cell biology", "developmental biology", "Organism development", "aging", "Evolutionary biology", "toxicology", "Neurotoxicology", "Toxic agents", "health care", "Environmental health", "Mental health and psychiatry", "dementia", "Alzheimer disease", "neurology", "Neurodegenerative diseases", "Public and occupational health", "Occupational and industrial medicine", "al", "toxicity", "neuroblastoma"], "article_id"=>1040891, "categories"=>["Biological Sciences"], "users"=>["Syed Husain Mustafa Rizvi", "Arshiya Parveen", "Anoop K. Verma", "Iqbal Ahmad", "Md Arshad", "Abbas Ali Mahdi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098409.g008", "stats"=>{"downloads"=>3, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Possible_signaling_mechanism_involved_during_Al_toxicity_in_human_neuroblastoma_cells_/1040891", "title"=>"Possible signaling mechanism involved during Al toxicity in human neuroblastoma cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-05-30 03:46:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1514909"], "description"=>"<p>List of primer sequences used in real time PCR.</p>", "links"=>[], "tags"=>["Biochemistry", "Neurochemistry", "cell biology", "Cell processes", "Cell death", "Cellular stress responses", "Molecular cell biology", "developmental biology", "Organism development", "aging", "Evolutionary biology", "toxicology", "Neurotoxicology", "Toxic agents", "health care", "Environmental health", "Mental health and psychiatry", "dementia", "Alzheimer disease", "neurology", "Neurodegenerative diseases", "Public and occupational health", "Occupational and industrial medicine", "primer", "sequences"], "article_id"=>1040892, "categories"=>["Biological Sciences"], "users"=>["Syed Husain Mustafa Rizvi", "Arshiya Parveen", "Anoop K. Verma", "Iqbal Ahmad", "Md Arshad", "Abbas Ali Mahdi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098409.t001", "stats"=>{"downloads"=>10, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_List_of_primer_sequences_used_in_real_time_PCR_/1040892", "title"=>"List of primer sequences used in real time PCR.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-05-30 03:46:29"}

PMC Usage Stats | Further Information

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{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Medicine and health sciences/Oncology", "average_usage"=>[266]}]}
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