A Comparison of Protein Kinases Inhibitor Screening Methods Using Both Enzymatic Activity and Binding Affinity Determination
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"A comparison of protein kinases inhibitor screening methods using both enzymatic activity and binding affinity determination", "type"=>"journal", "authors"=>[{"first_name"=>"Amalie Frederikke", "last_name"=>"Rudolf", "scopus_author_id"=>"56208915900"}, {"first_name"=>"Tine", "last_name"=>"Skovgaard", "scopus_author_id"=>"8519458900"}, {"first_name"=>"Stefan", "last_name"=>"Knapp", "scopus_author_id"=>"7202675553"}, {"first_name"=>"Lars Juhl", "last_name"=>"Jensen", "scopus_author_id"=>"35548941900"}, {"first_name"=>"Jens", "last_name"=>"Berthelsen", "scopus_author_id"=>"7004272677"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"373337794", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0098800", "scopus"=>"2-s2.0-84902579033", "pmid"=>"24915177", "sgr"=>"84902579033"}, "id"=>"f01e8681-8580-33ef-a03f-be595a41941e", "abstract"=>"Binding assays are increasingly used as a screening method for protein kinase inhibitors; however, as yet only a weak correlation with enzymatic activity-based assays has been demonstrated. We show that the correlation between the two types of assays can be improved using more precise screening conditions. Furthermore a marked improvement in the correlation was found by using kinase constructs containing the catalytic domain in presence of additional domains or subunits.", "link"=>"http://www.mendeley.com/research/comparison-protein-kinases-inhibitor-screening-methods-using-both-enzymatic-activity-binding-affinit-1", "reader_count"=>44, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>14, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>14, "Student > Master"=>7, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>14, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>14, "Student > Master"=>7, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>5, "Chemistry"=>12, "Psychology"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Chemistry"=>{"Chemistry"=>12}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>5}}, "reader_count_by_country"=>{"United States"=>3, "Indonesia"=>1}, "group_count"=>3}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84902579033"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84902579033?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84902579033&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84902579033&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84902579033", "dc:identifier"=>"SCOPUS_ID:84902579033", "eid"=>"2-s2.0-84902579033", "dc:title"=>"A comparison of protein kinases inhibitor screening methods using both enzymatic activity and binding affinity determination", "dc:creator"=>"Rudolf A.F.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"6", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-06-10", "prism:coverDisplayDate"=>"10 June 2014", "prism:doi"=>"10.1371/journal.pone.0098800", "citedby-count"=>"35", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Copenhagen, Faculty of Health Sciences", "affiliation-city"=>"Copenhagen", "affiliation-country"=>"Denmark"}], "pubmed-id"=>"24915177", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e98800", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0098800", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"53", "xml_views"=>"16", "html_views"=>"133"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"61"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"116"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"97"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"70"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"70"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"96"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"118"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"96"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"75"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"122"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"29", "xml_views"=>"1", "html_views"=>"167"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"184"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"142"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"119"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"144"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"22", "xml_views"=>"3", "html_views"=>"111"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"31", "xml_views"=>"1", "html_views"=>"129"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"37", "xml_views"=>"2", "html_views"=>"140"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"24", "xml_views"=>"2", "html_views"=>"187"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"166"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"103"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"49", "xml_views"=>"1", "html_views"=>"128"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"42", "xml_views"=>"3", "html_views"=>"121"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"52", "xml_views"=>"0", "html_views"=>"150"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"37", "xml_views"=>"0", "html_views"=>"108"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"37", "xml_views"=>"3", "html_views"=>"101"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"28", "xml_views"=>"1", "html_views"=>"92"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"31", "xml_views"=>"1", "html_views"=>"101"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"29", "xml_views"=>"2", "html_views"=>"128"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"39", "xml_views"=>"5", "html_views"=>"118"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"37", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"39", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"46", "xml_views"=>"0", "html_views"=>"131"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"79"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"28", "xml_views"=>"1", "html_views"=>"85"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"36", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"48", "xml_views"=>"0", "html_views"=>"140"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"82"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"30", "xml_views"=>"1", "html_views"=>"119"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"30", "xml_views"=>"1", "html_views"=>"77"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"35", "xml_views"=>"0", "html_views"=>"167"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"95", "xml_views"=>"0", "html_views"=>"132"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"116", "xml_views"=>"1", "html_views"=>"122"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"33", "xml_views"=>"1", "html_views"=>"93"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"35", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"30", "xml_views"=>"1", "html_views"=>"91"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"64"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1529475"], "description"=>"<p>Scatter plot comparison of our IC<sub>50</sub> and T<sub>m</sub> shift data divided into proteins with only the catalytic domains (<b>A</b>) and proteins with additional domains/subunits only (<b>B</b>). The dashed horizontal line corresponds to an IC<sub>50</sub> of 0.5 µM. The dashed vertical line in the plots of 4°C Tm shift corresponds to a binding affinity <1 µM. A significant correlation of binding and inhibition is observed (P<0.001).</p>", "links"=>[], "tags"=>["Biochemistry", "enzymology", "Enzyme kinetics", "enzymes", "Biomacromolecule-ligand interactions", "chemical biology", "proteins", "biophysics", "biotechnology", "Small molecules", "pharmacology", "Drug research and development", "drug discovery", "chemistry", "mathematics", "Statistics (mathematics)", "Biostatistics", "activity-based", "kinase-inhibitor"], "article_id"=>1052898, "categories"=>["Biological Sciences"], "users"=>["Amalie Frederikke Rudolf", "Tine Skovgaard", "Stefan Knapp", "Lars Juhl Jensen", "Jens Berthelsen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098800.g002", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Binding_and_activity_based_screening_data_comparison_based_on_kinase_inhibitor_pairs_/1052898", "title"=>"Binding and activity-based screening data comparison based on kinase-inhibitor pairs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-06-10 03:32:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/1529476"], "description"=>"<p>Protein kinase constructs and properties. Overview of the constructsused in this study, indicating protein name and Uniprot-ID (U-ID), protein domain contents and the corresponding amino acids of the relative Uniprot sequences (FL: full length; CD: catalytic domain). Also indicated is K<sub>m</sub> for ATP that we determined for each construct, the concentration of ATP present in the assay for each kinase, the amount of enzyme used and the assay substrate peptide sequence (5F: 5-FAM or 5- Fluorescein AMidite tag).</p>", "links"=>[], "tags"=>["Biochemistry", "enzymology", "Enzyme kinetics", "enzymes", "Biomacromolecule-ligand interactions", "chemical biology", "proteins", "biophysics", "biotechnology", "Small molecules", "pharmacology", "Drug research and development", "drug discovery", "chemistry", "mathematics", "Statistics (mathematics)", "Biostatistics", "kinase", "constructs"], "article_id"=>1052899, "categories"=>["Biological Sciences"], "users"=>["Amalie Frederikke Rudolf", "Tine Skovgaard", "Stefan Knapp", "Lars Juhl Jensen", "Jens Berthelsen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098800.t001", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Protein_kinase_constructs_and_properties_/1052899", "title"=>"Protein kinase constructs and properties.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-06-10 03:32:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/1529462"], "description"=>"<p><b>A</b>, Recreation of a scatter plot from Anastassiadis <i>et al</i>. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098800#pone.0098800-Anastassiadis1\" target=\"_blank\">[2]</a> with comparison of their data, % activity of control (Ctrl) at 0.5 µM compound with T<sub>m</sub> shift data obtained from Fedorov <i>et al. </i><a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098800#pone.0098800-Fedorov1\" target=\"_blank\">[5]</a>. (<b>B</b>) Scatter plot comparison of our IC<sub>50</sub> and T<sub>m</sub> shift data. The dashed horizontal line corresponds in <b>A</b> to 50% activity at 0.5 µM compound and in <b>B</b> to an IC<sub>50</sub> of 0.5 µM. The dashed vertical line in the plots of 4°C Tm shift corresponds to a binding affinity <1 µM. A significant correlation of binding and inhibition is observed for both analyses (P<0.001).</p>", "links"=>[], "tags"=>["Biochemistry", "enzymology", "Enzyme kinetics", "enzymes", "Biomacromolecule-ligand interactions", "chemical biology", "proteins", "biophysics", "biotechnology", "Small molecules", "pharmacology", "Drug research and development", "drug discovery", "chemistry", "mathematics", "Statistics (mathematics)", "Biostatistics", "activity-based", "kinase-inhibitor"], "article_id"=>1052885, "categories"=>["Biological Sciences"], "users"=>["Amalie Frederikke Rudolf", "Tine Skovgaard", "Stefan Knapp", "Lars Juhl Jensen", "Jens Berthelsen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0098800.g001", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Binding_and_activity_based_screening_data_comparison_based_on_kinase_inhibitor_pairs_/1052885", "title"=>"Binding and activity-based screening data comparison based on kinase-inhibitor pairs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-06-10 03:32:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/1529483", "https://ndownloader.figshare.com/files/1529484", "https://ndownloader.figshare.com/files/1529485"], "description"=>"<div><p>Binding assays are increasingly used as a screening method for protein kinase inhibitors; however, as yet only a weak correlation with enzymatic activity-based assays has been demonstrated. We show that the correlation between the two types of assays can be improved using more precise screening conditions. Furthermore a marked improvement in the correlation was found by using kinase constructs containing the catalytic domain in presence of additional domains or subunits.</p></div>", "links"=>[], "tags"=>["Biochemistry", "enzymology", "Enzyme kinetics", "enzymes", "Biomacromolecule-ligand interactions", "chemical biology", "proteins", "biophysics", "biotechnology", "Small molecules", "pharmacology", "Drug research and development", "drug discovery", "chemistry", "mathematics", "Statistics (mathematics)", "Biostatistics", "kinases", "inhibitor", "methods", "enzymatic", "binding", "affinity"], "article_id"=>1052902, "categories"=>["Biological Sciences"], "users"=>["Amalie Frederikke Rudolf", "Tine Skovgaard", "Stefan Knapp", "Lars Juhl Jensen", "Jens Berthelsen"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0098800.s001", "https://dx.doi.org/10.1371/journal.pone.0098800.s002", "https://dx.doi.org/10.1371/journal.pone.0098800.s003"], "stats"=>{"downloads"=>5, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_Comparison_of_Protein_Kinases_Inhibitor_Screening_Methods_Using_Both_Enzymatic_Activity_and_Binding_Affinity_Determination_/1052902", "title"=>"A Comparison of Protein Kinases Inhibitor Screening Methods Using Both Enzymatic Activity and Binding Affinity Determination", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-06-10 03:32:54"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"22", "full-text"=>"13", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"28", "full-text"=>"9", "pdf"=>"21", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"23", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"22", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"20", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"27", "full-text"=>"29", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"14", "full-text"=>"8", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"28", "full-text"=>"30", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"24", "full-text"=>"26", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"22", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"29", "full-text"=>"27", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[292, 461]}]}
Loading … Spinner
There are currently no alerts