Inhibition of Catalase by Tea Catechins in Free and Cellular State: A Biophysical Approach
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{"title"=>"Inhibition of catalase by tea catechins in free and cellular state: A biophysical approach", "type"=>"journal", "authors"=>[{"first_name"=>"Sandip", "last_name"=>"Pal", "scopus_author_id"=>"56664790600"}, {"first_name"=>"Subrata Kumar", "last_name"=>"Dey", "scopus_author_id"=>"35466926900"}, {"first_name"=>"Chabita", "last_name"=>"Saha", "scopus_author_id"=>"55083310700"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"25025898", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0102460", "pui"=>"373524948", "scopus"=>"2-s2.0-84904263428", "sgr"=>"84904263428"}, "id"=>"891aa93d-f54a-3de2-950b-f9f1a524c50e", "abstract"=>"Tea flavonoids bind to variety of enzymes and inhibit their activities. In the present study, binding and inhibition of catalase activity by catechins with respect to their structure-affinity relationship has been elucidated. Fluorimetrically determined binding constants for (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG) with catalase were observed to be 2.27×106 M(-1) and 1.66×106 M(-1), respectively. Thermodynamic parameters evidence exothermic and spontaneous interaction between catechins and catalase. Major forces of interaction are suggested to be through hydrogen bonding along with electrostatic contributions and conformational changes. Distinct loss of α-helical structure of catalase by interaction with EGCG was captured in circular dichroism (CD) spectra. Gallated catechins demonstrated higher binding constants and inhibition efficacy than non-gallated catechins. EGCG exhibited maximum inhibition of pure catalase. It also inhibited cellular catalase in K562 cancer cells with significant increase in cellular ROS and suppression of cell viability (IC50 54.5 µM). These results decipher the molecular mechanism by which tea catechins interact with catalase and highlight the potential of gallated catechin like EGCG as an anticancer drug. EGCG may have other non-specific targets in the cell, but its anticancer property is mainly defined by ROS accumulation due to catalase inhibition.", "link"=>"http://www.mendeley.com/research/inhibition-catalase-tea-catechins-free-cellular-state-biophysical-approach", "reader_count"=>23, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>2, "Other"=>2, "Student > Master"=>4, "Student > Bachelor"=>2, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>2, "Other"=>2, "Student > Master"=>4, "Student > Bachelor"=>2, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>9, "Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>2, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Physics and Astronomy"=>1, "Chemistry"=>4, "Psychology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>4}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>9}, "Unspecified"=>{"Unspecified"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Indonesia"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1592669"], "description"=>"<p>The curves (a to f) represent pure catalase, EGC-catalase, EC-catalase, ECG-catalase, EGCG-catalase, and 3-AT-catalase complexes, respectively.</p>", "links"=>[], "tags"=>["Biochemistry", "antioxidants", "biocatalysis", "proteins", "biophysics", "cd", "spectra", "catalase", "complexes", "catechins", "3-at"], "article_id"=>1103692, "categories"=>["Biological Sciences"], "users"=>["Sandip Pal", "Subrata Kumar Dey", "Chabita Saha"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102460.g005", "stats"=>{"downloads"=>2, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_CD_spectra_of_catalase_1_181_M_and_its_complexes_with_catechins_and_3_AT_3_181_M_each_at_25_176_C_/1103692", "title"=>"The CD spectra of catalase (1 µM) and its complexes with catechins and 3-AT (3 µM each) at 25°C.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-15 03:01:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1592649"], "description"=>"<p>Curves (1 to 8) denote 2.5×10<sup>−9</sup> M to 1.7×10<sup>−8</sup> M of catechins.</p>", "links"=>[], "tags"=>["Biochemistry", "antioxidants", "biocatalysis", "proteins", "biophysics", "fluorescence", "spectra", "catalase", "quenching", "catechins"], "article_id"=>1103674, "categories"=>["Biological Sciences"], "users"=>["Sandip Pal", "Subrata Kumar Dey", "Chabita Saha"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102460.g002", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_fluorescence_emission_spectra_of_native_catalase_5_10_8722_7_M_and_the_quenching_effect_of_catechins_on_its_fluorescence_intensity_at_25_176_C_955_ex_8202_8202_280_nm_A_EC_catalase_B_EGC_catalase_C_ECG_catalase_and_D_EGCG_catalase_/1103674", "title"=>"The fluorescence emission spectra of native catalase (5×10<sup>−7</sup> M) and the quenching effect of catechins on its fluorescence intensity at 25°C (λ<sub>ex</sub> = 280 nm): (A) EC-catalase, (B) EGC-catalase, (C) ECG-catalase, and (D) EGCG-catalase.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-15 03:01:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1592662"], "description"=>"<p>ITC profiles for catalase (1 µM) when titrated with catechins (10 µM each) at 25°C: (A) EC-catalase, (B) EGC-catalase, (C) ECG-catalase, and (D) EGCG-catalase systems.</p>", "links"=>[], "tags"=>["Biochemistry", "antioxidants", "biocatalysis", "proteins", "biophysics", "profiles", "catalase", "titrated", "catechins", "egcg-catalase"], "article_id"=>1103687, "categories"=>["Biological Sciences"], "users"=>["Sandip Pal", "Subrata Kumar Dey", "Chabita Saha"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102460.g004", "stats"=>{"downloads"=>2, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ITC_profiles_for_catalase_1_181_M_when_titrated_with_catechins_10_181_M_each_at_25_176_C_A_EC_catalase_B_EGC_catalase_C_ECG_catalase_and_D_EGCG_catalase_systems_/1103687", "title"=>"ITC profiles for catalase (1 µM) when titrated with catechins (10 µM each) at 25°C: (A) EC-catalase, (B) EGC-catalase, (C) ECG-catalase, and (D) EGCG-catalase systems.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-15 03:01:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1592674"], "description"=>"a<p>Calculated from the average values of Δ<i>H</i>.</p>b<p>Directly extracted from the experiment.</p>", "links"=>[], "tags"=>["Biochemistry", "antioxidants", "biocatalysis", "proteins", "biophysics", "itc", "experiments", "catalase-catechins"], "article_id"=>1103697, "categories"=>["Biological Sciences"], "users"=>["Sandip Pal", "Subrata Kumar Dey", "Chabita Saha"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102460.t001", "stats"=>{"downloads"=>4, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Thermodynamic_parameters_from_ITC_experiments_for_catalase_catechins_system_at_25_176_C_/1103697", "title"=>"Thermodynamic parameters from ITC experiments for catalase-catechins system at 25°C.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-07-15 03:01:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1592672"], "description"=>"<p>Double reciprocal plot or Lineweaver-Burk plot of catalase – H<sub>2</sub>O<sub>2</sub> (▪) and catalase+EGCG – H<sub>2</sub>O<sub>2</sub> (*) reactions.</p>", "links"=>[], "tags"=>["Biochemistry", "antioxidants", "biocatalysis", "proteins", "biophysics", "reciprocal", "lineweaver-burk", "catalase"], "article_id"=>1103695, "categories"=>["Biological Sciences"], "users"=>["Sandip Pal", "Subrata Kumar Dey", "Chabita Saha"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102460.g007", "stats"=>{"downloads"=>3, "page_views"=>128, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Double_reciprocal_plot_or_Lineweaver_Burk_plot_of_catalase_H_2_O_2_9642_and_catalase_EGCG_8211_H_2_O_2_reactions_/1103695", "title"=>"Double reciprocal plot or Lineweaver-Burk plot of catalase – H<sub>2</sub>O<sub>2</sub> (▪) and catalase+EGCG – H<sub>2</sub>O<sub>2</sub> (*) reactions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-15 03:01:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1592673"], "description"=>"<p>(*<i>p</i><0.05 as compared to control group).</p>", "links"=>[], "tags"=>["Biochemistry", "antioxidants", "biocatalysis", "proteins", "biophysics", "intracellular", "ros", "concentrations"], "article_id"=>1103696, "categories"=>["Biological Sciences"], "users"=>["Sandip Pal", "Subrata Kumar Dey", "Chabita Saha"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102460.g008", "stats"=>{"downloads"=>5, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Histograms_representing_the_intracellular_ROS_content_in_absence_and_presence_of_various_concentrations_12_5_8211_100_181_M_of_EGCG_/1103696", "title"=>"Histograms representing the intracellular ROS content in absence and presence of various concentrations (12.5–100 µM) of EGCG.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-15 03:01:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1592652"], "description"=>"<p>(A) EC-catalase, (B) EGC-catalase, (C) ECG-catalase, and (D) EGCG-catalase.</p>", "links"=>[], "tags"=>["Biochemistry", "antioxidants", "biocatalysis", "proteins", "biophysics", "logarithmic", "catalase-catechins"], "article_id"=>1103677, "categories"=>["Biological Sciences"], "users"=>["Sandip Pal", "Subrata Kumar Dey", "Chabita Saha"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102460.g003", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Double_logarithmic_plot_of_catalase_catechins_system_at_25_C_ex_8202_8202_280_nm_/1103677", "title"=>"Double logarithmic plot of catalase-catechins system at 25°C (λ<sub>ex</sub> = 280 nm).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-15 03:01:56"}
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PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Nutrition", "average_usage"=>[289]}, {"subject_area"=>"/Medicine and health sciences/Nutrition", "average_usage"=>[285]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[271]}, {"subject_area"=>"/Physical sciences/Physics", "average_usage"=>[266]}]}
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