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• {"link"=>{"url"=>"http://www.citeulike.org/user/hongenxu/article/13555510"}, "post_time"=>"2015-03-19 13:32:11", "tag"=>["cancer", "dnaconformation", "g-quadruplex"], "linkout"=>[{"type"=>"DOI", "url"=>"http://dx.doi.org/10.1371/journal.pone.0102711"}, {"type"=>"Pubmed", "url"=>"http://view.ncbi.nlm.nih.gov/pubmed/25033211"}, {"type"=>"Hubmed", "url"=>"http://www.hubmed.org/display.cgi?uids=25033211"}], "username"=>"hongenxu", "article_id"=>"13555510"}

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{"title"=>"Elevated levels of G-quadruplex formation in human stomach and liver cancer tissues", "type"=>"journal", "authors"=>[{"first_name"=>"Giulia", "last_name"=>"Biffi", "scopus_author_id"=>"55320486800"}, {"first_name"=>"David", "last_name"=>"Tannahill", "scopus_author_id"=>"6701802836"}, {"first_name"=>"Jodi", "last_name"=>"Miller", "scopus_author_id"=>"56278330000"}, {"first_name"=>"William J.", "last_name"=>"Howat", "scopus_author_id"=>"6506020964"}, {"first_name"=>"Shankar", "last_name"=>"Balasubramanian", "scopus_author_id"=>"7201550638"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"373567457", "sgr"=>"84904489184", "issn"=>"19326203", "pmid"=>"25033211", "scopus"=>"2-s2.0-84904489184", "doi"=>"10.1371/journal.pone.0102711", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)"}, "id"=>"3cc42d66-daea-3961-a7e7-77b1dcacd6b3", "abstract"=>"Four-stranded G-quadruplex DNA secondary structures have recently been visualized in the nuclei of human cultured cells. Here, we show that BG4, a G-quadruplex-specific antibody, can be used to stain DNA G-quadruplex structures in patient-derived tissues using immunohistochemistry. We observe a significantly elevated number of G-quadruplex-positive nuclei in human cancers of the liver and stomach as compared to background non-neoplastic tissue. Our results suggest that G-quadruplex formation can be detected and measured in patient-derived material and that elevated G-quadruplex formation may be a characteristic of some cancers.", "link"=>"http://www.mendeley.com/research/elevated-levels-gquadruplex-formation-human-stomach-liver-cancer-tissues", "reader_count"=>27, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>3, "Researcher"=>8, "Student > Ph. D. Student"=>6, "Other"=>1, "Student > Master"=>4, "Student > Bachelor"=>3}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>3, "Researcher"=>8, "Student > Ph. D. Student"=>6, "Other"=>1, "Student > Master"=>4, "Student > Bachelor"=>3}, "reader_count_by_subject_area"=>{"Nursing and Health Professions"=>1, "Biochemistry, Genetics and Molecular Biology"=>7, "Agricultural and Biological Sciences"=>12, "Medicine and Dentistry"=>1, "Chemistry"=>6}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>6}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>12}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>7}}, "reader_count_by_country"=>{"United Kingdom"=>1, "France"=>1, "Switzerland"=>2}, "group_count"=>1}

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• http://doi.org/10.1002/anie.201500891
• http://doi.org/10.1074/jbc.RA118.004499
• http://doi.org/10.1038/s41598-017-00739-y
• http://doi.org/10.1038/ncomms14432
• http://doi.org/10.1038/srep28211
• http://doi.org/10.1134/S0006297919050109
• http://doi.org/10.1021/bi5014722
• http://doi.org/10.1016/j.dnarep.2016.08.001
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• http://doi.org/10.1093/nar/gkz179
• http://doi.org/10.1016/j.trechm.2019.07.002
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• http://doi.org/10.1134/S0006297916130034
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• http://doi.org/10.1093/nar/gkv427
• http://doi.org/10.1021/acs.jmedchem.7b01781

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Figshare

• {"files"=>["https://ndownloader.figshare.com/files/1599259"], "description"=>"<p>Background non-neoplastic and cancer liver tissues were stained by IHC using the G-quadruplex-specific antibody BG4. <b>A</b>. The nuclei of non-neoplastic liver tissue from a hepatocellular carcinoma patient are mostly BG4-negative, with haematoxylin counterstaining (blue) evident. Scale bar corresponds to 50 µm. Note that the cytoplasmic granules are variable and likely to be heme-based artifacts sometimes observed in liver IHC. <b>B</b>. Neoplastic tissue from the same patient in <b>A</b> shows the extensive presence of BG4-positive nuclei (brown). <b>C</b>. The nuclei of non-neoplastic liver tissue for an intrahepatic cholangiocarcinoma patient are mostly BG4-negative. <b>D</b>. Neoplastic tissue from the same patient in <b>C</b> shows many BG4-positive nuclei. <b>E</b>. The nuclei of non-neoplastic liver tissue from a metastatic large cell undifferentiated lung carcinoma patient are mostly BG4-negative. <b>F</b>. Metastatic liver tissue isolated from the same patient in <b>E</b> shows many BG4-positive nuclei. <b>G</b>. Quantification of the number of BG4-positive nuclei in liver cancer and corresponding matched non-neoplastic tissues from nine patients. Error bars represent the standard error of the mean (s.e.m.) calculated when duplicate TMA cores were available. Overall, these results indicate that there are a greater number of G-quadruplex structures in liver cancer compared to background non-neoplastic tissue from the same individual.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA structure", "chemical biology", "genetics", "molecular biology", "Macromolecular structure analysis", "oncology", "Cancer risk factors", "Genetic causes of cancer", "Basic cancer research", "chemistry", "incidence", "g-quadruplex", "structures", "cancer", "matched", "non-neoplastic"], "article_id"=>1109211, "categories"=>["Biological Sciences"], "users"=>["Giulia Biffi", "David Tannahill", "Jodi Miller", "William J. Howat", "Shankar Balasubramanian"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102711.g002", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Increased_incidence_of_DNA_G_quadruplex_structures_in_human_liver_cancer_relative_to_matched_non_neoplastic_tissue_/1109211", "title"=>"Increased incidence of DNA G-quadruplex structures in human liver cancer relative to matched non-neoplastic tissue.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-17 03:47:58"}
• {"files"=>["https://ndownloader.figshare.com/files/1599308"], "description"=>"<p>Gastric adenocarcinoma and signet ring cell carcinoma and matched background non-neoplastic tissues taken from the same patient were stained with the G-quadruplex-specific antibody BG4 by IHC. <b>A</b>. The nuclei of non-neoplastic stomach tissue from a gastric adenocarcinoma patient are mostly BG4-negative, with haematoxylin counterstaining (blue) readily apparent. Scale bar corresponds to 50 µm. <b>B</b>. Adenocarcinoma tissue from the same patient in <b>A</b> shows extensive numbers of BG4-positive nuclei (brown). <b>C</b>. The nuclei of non-neoplastic stomach tissue from a signet ring cell carcinoma patient are mostly BG4-negative. <b>D</b>. Signet ring cell carcinoma tissue from the same patient in <b>C</b> shows many BG4-positive nuclei. <b>E</b>. Quantification of the number of BG4-positive nuclei in the matched stomach cancers and background non-neoplastic tissues from two different patients. Error bars represent the s.e.m. calculated when duplicate TMA cores were available. These results indicate that there are more G-quadruplex structures in the nuclei of stomach cancer tissue than in non-neoplastic tissue from the same individual.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA structure", "chemical biology", "genetics", "molecular biology", "Macromolecular structure analysis", "oncology", "Cancer risk factors", "Genetic causes of cancer", "Basic cancer research", "chemistry", "incidence", "g-quadruplex", "structures", "cancer", "matched", "non-neoplastic"], "article_id"=>1109255, "categories"=>["Biological Sciences"], "users"=>["Giulia Biffi", "David Tannahill", "Jodi Miller", "William J. Howat", "Shankar Balasubramanian"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102711.g004", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Increased_incidence_of_DNA_G_quadruplex_structures_in_human_stomach_cancer_relative_to_matched_non_neoplastic_tissue_/1109255", "title"=>"Increased incidence of DNA G-quadruplex structures in human stomach cancer relative to matched non-neoplastic tissue.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-17 03:47:58"}
• {"files"=>["https://ndownloader.figshare.com/files/1599216"], "description"=>"<p><b>A</b>. Human MDA-MB-231 breast adenocarcinoma cell pellets were fixed, paraffin embedded and processed for IHC using the G-quadruplex-specific antibody BG4. No staining is observed in the absence of epitope retrieval. Scale bar corresponds to 20 µm. Nuclei are counterstained with haematoxylin (blue). <b>B</b>. Strong BG4 staining (brown) is readily apparent in cell nuclei following antigen retrieval with citrate-based buffer pH 6.0. <b>C</b>. No staining is observed in the absence of the BG4 primary antibody. <b>D</b>. No staining is seen following DNase treatment prior to BG4 staining. These results show that BG4 can be employed for DNA G-quadruplex detection by IHC staining.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA structure", "chemical biology", "genetics", "molecular biology", "Macromolecular structure analysis", "oncology", "Cancer risk factors", "Genetic causes of cancer", "Basic cancer research", "chemistry", "g-quadruplex", "staining"], "article_id"=>1109193, "categories"=>["Biological Sciences"], "users"=>["Giulia Biffi", "David Tannahill", "Jodi Miller", "William J. Howat", "Shankar Balasubramanian"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102711.g001", "stats"=>{"downloads"=>4, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Validation_of_G_quadruplex_nuclear_staining_by_immunohistochemistry_/1109193", "title"=>"Validation of G-quadruplex nuclear staining by immunohistochemistry.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-17 03:47:58"}
• {"files"=>["https://ndownloader.figshare.com/files/1599274"], "description"=>"<p><b>A</b>. Quantification of the number of BG4-positive nuclei in individual liver tissues including unmatched TMA cores. Non-neoplastic liver shows less BG4-positive nuclei compared to hepatocellular carcinoma or intrahepatic cholangiocarcinoma. Each column corresponds to a single tissue sample and error bars represent the s.e.m. calculated when duplicate tissue samples were available. <b>B</b>. Overall quantification of the number of BG4-positive nuclei in all non-neoplastic and cancer liver tissues. Error bars represent the s.e.m. **P<0.001, n = 19 and 32 for non-neoplastic and cancer cores, respectively. These results confirm the generality of more extensive G-quadruplex formation in the liver cancers compared to non-neoplastic tissues.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA structure", "chemical biology", "genetics", "molecular biology", "Macromolecular structure analysis", "oncology", "Cancer risk factors", "Genetic causes of cancer", "Basic cancer research", "chemistry", "incidence", "g-quadruplex-positive", "nuclei"], "article_id"=>1109226, "categories"=>["Biological Sciences"], "users"=>["Giulia Biffi", "David Tannahill", "Jodi Miller", "William J. Howat", "Shankar Balasubramanian"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102711.g003", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Increased_incidence_of_G_quadruplex_positive_cell_nuclei_across_human_liver_cancers_/1109226", "title"=>"Increased incidence of G-quadruplex-positive cell nuclei across human liver cancers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-17 03:47:58"}
• {"files"=>["https://ndownloader.figshare.com/files/1599312"], "description"=>"<p>Confirmation of the increased presence of BG4-positive nuclei in a range of stomach cancer tissues compared to background non-neoplastic tissues including unmatched cases. <b>A</b>. The nuclei of non-neoplastic stomach tissues are largely BG4-negative. Cell nuclei were counterstained with haematoxylin (blue). Scale bar corresponds to 50 µm. <b>B</b>. Gastric adenocarcinoma tissue shows the extensive presence of BG4-positive nuclei (brown). <b>C</b>. Signet ring cell carcinoma shows many BG4-positive nuclei. <b>D</b>. Gastrointestinal stromal tumors (GIST) are largely BG4-positive (for the survey of stomach cancer only unmatched GIST tissues were available). <b>E</b>. Quantification of the number of BG4-positive nuclei in individual stomach tissues including unmatched TMA cores. Non-neoplastic stomach tissue shows less BG4-positive nuclei compared to adenocarcinoma, signet ring carcinoma or GIST. Each column corresponds to a single tissue sample and error bars represent the s.e.m. calculated when duplicate tissue samples were available. <b>F</b>. Overall quantification of the number of BG4-positive nuclei in all non-neoplastic and stomach liver tissues. Error bars represent the s.e.m. **P<0.001, n = 10 and 34 for non-neoplastic and cancer cores, respectively. These results confirm the generality of more extensive G-quadruplex formation in the stomach cancers compared t­o non-neoplastic tissues.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA structure", "chemical biology", "genetics", "molecular biology", "Macromolecular structure analysis", "oncology", "Cancer risk factors", "Genetic causes of cancer", "Basic cancer research", "chemistry", "incidence", "g-quadruplex-positive", "nuclei"], "article_id"=>1109259, "categories"=>["Biological Sciences"], "users"=>["Giulia Biffi", "David Tannahill", "Jodi Miller", "William J. Howat", "Shankar Balasubramanian"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102711.g005", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Increased_incidence_of_G_quadruplex_positive_cell_nuclei_across_human_stomach_cancers_/1109259", "title"=>"Increased incidence of G-quadruplex-positive cell nuclei across human stomach cancers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-17 03:47:58"}
• {"files"=>["https://ndownloader.figshare.com/files/1599331", "https://ndownloader.figshare.com/files/1599332", "https://ndownloader.figshare.com/files/1599333"], "description"=>"<div><p>Four-stranded G-quadruplex DNA secondary structures have recently been visualized in the nuclei of human cultured cells. Here, we show that BG4, a G-quadruplex-specific antibody, can be used to stain DNA G-quadruplex structures in patient-derived tissues using immunohistochemistry. We observe a significantly elevated number of G-quadruplex-positive nuclei in human cancers of the liver and stomach as compared to background non-neoplastic tissue. Our results suggest that G-quadruplex formation can be detected and measured in patient-derived material and that elevated G-quadruplex formation may be a characteristic of some cancers.</p></div>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA structure", "chemical biology", "genetics", "molecular biology", "Macromolecular structure analysis", "oncology", "Cancer risk factors", "Genetic causes of cancer", "Basic cancer research", "chemistry", "g-quadruplex", "cancer"], "article_id"=>1109274, "categories"=>["Biological Sciences"], "users"=>["Giulia Biffi", "David Tannahill", "Jodi Miller", "William J. Howat", "Shankar Balasubramanian"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0102711.s001", "https://dx.doi.org/10.1371/journal.pone.0102711.s002", "https://dx.doi.org/10.1371/journal.pone.0102711.s003"], "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Elevated_Levels_of_G_Quadruplex_Formation_in_Human_Stomach_and_Liver_Cancer_Tissues_/1109274", "title"=>"Elevated Levels of G-Quadruplex Formation in Human Stomach and Liver Cancer Tissues", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-07-17 03:47:58"}

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Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[267]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[306, 482]}, {"subject_area"=>"/Medicine and health sciences/Gastroenterology and hepatology", "average_usage"=>[246]}]}

F1000Prime