The Octopamine Receptor Octβ2R Regulates Ovulation in Drosophila melanogaster
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"The octopamine receptor Octβ2R regulates ovulation in Drosophila melanogaster", "type"=>"journal", "authors"=>[{"first_name"=>"Junghwa", "last_name"=>"Lim", "scopus_author_id"=>"55566618600"}, {"first_name"=>"Paul R.", "last_name"=>"Sabandal", "scopus_author_id"=>"56311201300"}, {"first_name"=>"Ana", "last_name"=>"Fernandez", "scopus_author_id"=>"57194531746"}, {"first_name"=>"John Martin", "last_name"=>"Sabandal", "scopus_author_id"=>"56311377600"}, {"first_name"=>"Hyun Gwan", "last_name"=>"Lee", "scopus_author_id"=>"8660554600"}, {"first_name"=>"Peter", "last_name"=>"Evans", "scopus_author_id"=>"7402236618"}, {"first_name"=>"Kyung An", "last_name"=>"Han", "scopus_author_id"=>"7402963645"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84905460197", "sgr"=>"84905460197", "pui"=>"373700888", "isbn"=>"1932-6203", "pmid"=>"25099506", "doi"=>"10.1371/journal.pone.0104441"}, "id"=>"ed442a20-834d-3d98-bb16-f62b7b133ccc", "abstract"=>"Oviposition is induced upon mating in most insects. Ovulation is a primary step in oviposition, representing an important target to control insect pests and vectors, but limited information is available on the underlying mechanism. Here we report that the beta adrenergic-like octopamine receptor Octβ2R serves as a key signaling molecule for ovulation and recruits protein kinase A and Ca(2+)/calmodulin-sensitive kinase II as downstream effectors for this activity. We found that the octβ2r homozygous mutant females are sterile. They displayed normal courtship, copulation, sperm storage and post-mating rejection behavior but were unable to lay eggs. We have previously shown that octopamine neurons in the abdominal ganglion innervate the oviduct epithelium. Consistently, restored expression of Octβ2R in oviduct epithelial cells was sufficient to reinstate ovulation and full fecundity in the octβ2r mutant females, demonstrating that the oviduct epithelium is a major site of Octβ2R's function in oviposition. We also found that overexpression of the protein kinase A catalytic subunit or Ca(2+)/calmodulin-sensitive protein kinase II led to partial rescue of octβ2r's sterility. This suggests that Octβ2R activates cAMP as well as additional effectors including Ca(2+)/calmodulin-sensitive protein kinase II for oviposition. All three known beta adrenergic-like octopamine receptors stimulate cAMP production in vitro. Octβ1R, when ectopically expressed in the octβ2r's oviduct epithelium, fully reinstated ovulation and fecundity. Ectopically expressed Octβ3R, on the other hand, partly restored ovulation and fecundity while OAMB-K3 and OAMB-AS that increase Ca(2+) levels yielded partial rescue of ovulation but not fecundity deficit. These observations suggest that Octβ2R have distinct signaling capacities in vivo and activate multiple signaling pathways to induce egg laying. The findings reported here narrow the knowledge gap and offer insight into novel strategies for insect control.", "link"=>"http://www.mendeley.com/research/octopamine-receptor-oct%CE%B22r-regulates-ovulation-drosophila-melanogaster", "reader_count"=>32, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>2, "Student > Master"=>9, "Other"=>1, "Student > Bachelor"=>3, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>2, "Student > Master"=>9, "Other"=>1, "Student > Bachelor"=>3, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>23, "Neuroscience"=>4}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Neuroscience"=>{"Neuroscience"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>23}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}}, "reader_count_by_country"=>{"Canada"=>1, "Portugal"=>1}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84905460197"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84905460197?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84905460197&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84905460197&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84905460197", "dc:identifier"=>"SCOPUS_ID:84905460197", "eid"=>"2-s2.0-84905460197", "dc:title"=>"The octopamine receptor Octβ2R regulates ovulation in Drosophila melanogaster", "dc:creator"=>"Lim J.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-08-06", "prism:coverDisplayDate"=>"6 August 2014", "prism:doi"=>"10.1371/journal.pone.0104441", "citedby-count"=>"33", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"The University of Texas at El Paso", "affiliation-city"=>"El Paso", "affiliation-country"=>"United States"}], "pubmed-id"=>"25099506", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e104441", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true, "freetoread"=>{"value"=>[{"$"=>"all"}, {"$"=>"publisherfullgold"}, {"$"=>"repository"}, {"$"=>"repositoryvor"}, {"$"=>"repositoryam"}]}, "freetoreadLabel"=>{"value"=>[{"$"=>"All Open Access"}, {"$"=>"Gold"}, {"$"=>"Green"}]}}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0104441", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"75", "xml_views"=>"8", "html_views"=>"280"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"70"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"117"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"60"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"2", "html_views"=>"44"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"57"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"42"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"58"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"4", "html_views"=>"40"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"5", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"4", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"4", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"20", "xml_views"=>"2", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"14", "xml_views"=>"3", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"22", "xml_views"=>"2", "html_views"=>"26"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2020", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2020", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2021", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"14"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1623403"], "description"=>"<p>(A) Copulation behavior. Virgin wild-type <i>CS</i> or <i>octβ2r</i> females were singly paired with naïve <i>CS</i> males to examine copulation behavior. The <i>CS</i> males paired with <i>octβ2r</i> females exhibited copulation latency and duration comparable to those paired with <i>CS</i> females (<i>p</i>>0.05 by Student’s t-test; <i>n</i> = 17–24). (B) Sperm retention. <i>CS</i> and <i>octβ2r</i> females were mated with <i>dj-GFP</i> males carrying GFP-tagged sperm (green) and the reproductive systems were dissected and counterstained with DAPI (blue). <i>CS</i> and <i>octβ2r</i> females had comparable sperm storage in the seminal receptacle and spermathecae at 24 and 48 h after mating. Spt, spermathecae; SmRcp, seminal receptacle. Scale bar, 50 µm. (C) Ovulation. <i>CS</i> and <i>octβ2r</i> females were mated with <i>CS</i> males and the reproductive systems were dissected to examine the location of the egg in the oviduct or uterus. The <i>octβ2r</i> females had significantly lower levels of ovulation than <i>CS</i> females (***, <i>p</i><0.0001 by Student’s t-tests; <i>n</i> = 10). (D) Courtship behavior. Virgin <i>CS</i> and <i>octβ2r</i> females were singly paired with naïve <i>CS</i> males to measure courtship activity. The mated females were tested again with new naïve males 48 h later to measure courtship receptivity. The percentage of time that the CS males spent courting <i>CS</i> or <i>octβ2r</i> females represents courtship index. With both <i>CS</i> and <i>octβ2r</i> females, courtship activities of the males paired with mated females were significant lower than those of the males paired with virgin females (<i>p</i><0.0001 by Mann-Whitney; <i>n</i> = 32–69). Thus, <i>CS</i> and <i>octβ2r</i> females have comparable pre- and post-mating courtship activity. ns, not significant.</p>", "links"=>[], "tags"=>["anatomy", "Reproductive system", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "physiology", "Reproductive physiology", "Model organisms", "Animal models", "sterility", "phenotype", "mutant", "defective"], "article_id"=>1129216, "categories"=>["Biological Sciences"], "users"=>["Junghwa Lim", "Paul R. Sabandal", "Ana Fernández", "John Martin Sabandal", "Hyun-Gwan Lee", "Peter Evans", "Kyung-An Han"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104441.g001", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_sterility_phenotype_of_the_oct_946_2r_mutant_female_is_due_to_defective_ovulation_/1129216", "title"=>"The sterility phenotype of the <i>octβ2r</i> mutant female is due to defective ovulation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-08-06 03:04:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1623415"], "description"=>"<p>Mating activates the OA neurons that project to the oviduct epithelium. Binding of OA to the G-protein coupled receptors Octβ2R and OAMB in the epithelium induces cellular activity critical for egg transport from the ovary to the uterus. Specifically, CaMKII activated by Octβ2R together with OAMB may act on nitric oxide synthase (NOS) to release NO, which diffuses to the muscle for relaxation. Concerted activities of PKA and CaMKII activated by Octβ2R, on the other hand, trigger downstream effectors to secrete fluid for egg activation and transport.</p>", "links"=>[], "tags"=>["anatomy", "Reproductive system", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "physiology", "Reproductive physiology", "Model organisms", "Animal models", "ovulation"], "article_id"=>1129228, "categories"=>["Biological Sciences"], "users"=>["Junghwa Lim", "Paul R. Sabandal", "Ana Fernández", "John Martin Sabandal", "Hyun-Gwan Lee", "Peter Evans", "Kyung-An Han"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104441.g007", "stats"=>{"downloads"=>5, "page_views"=>45, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Working_model_of_ovulation_mechanism_/1129228", "title"=>"Working model of ovulation mechanism.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-08-06 03:04:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1623412"], "description"=>"<p>Transgenic expression of the OA receptors Octβ1R, Octβ3R, OAMB-K3 and OAMB-AS was driven by RS-GAL4 in the <i>octβ2r</i>’s oviduct epithelium. (A) Ovulation rescue. Ectopically expressed <i>Octβ1R</i> fully rescued the <i>octβ2r</i>’s ovulation phenotype while <i>Octβ3R</i>, <i>OAMB-K3</i> and <i>OAMB-AS</i> yielded partial rescue (***, <i>p</i><0.0001; **, <i>p</i><0.005; ns, not significant; <i>n</i> = 18–34). (B) Fecundity rescue. Ectopic expression of <i>Octβ1R</i> fully restored fecundity whereas partial or no rescue was observed with ectopic expression of Octβ3R or OAMB-K3 and OAMB-AS, respectively (***, <i>p</i><0.0001, <i>n</i> = 20–38). (C) RT-PCR analysis. RNA was isolated from dissected reproductive tissues of <i>CS</i>, <i>octβ2r</i> mutant females carrying RS-GAL4 alone, and <i>octβ2r</i> mutant females carrying RS-GAL4 and UAS- Octβ1R or UAS-Octβ3R for RT-PCR. The elevated levels of Octβ1R or Octβ3R PCR products were detectable in the <i>octβ2r</i> females carrying RS-GAL4 and UAS- Octβ1R or UAS-Octβ3R, respectively. Rp49 was used for an internal control.</p>", "links"=>[], "tags"=>["anatomy", "Reproductive system", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "physiology", "Reproductive physiology", "Model organisms", "Animal models", "substitution", "oa"], "article_id"=>1129225, "categories"=>["Biological Sciences"], "users"=>["Junghwa Lim", "Paul R. Sabandal", "Ana Fernández", "John Martin Sabandal", "Hyun-Gwan Lee", "Peter Evans", "Kyung-An Han"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104441.g005", "stats"=>{"downloads"=>5, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Functional_substitution_by_other_OA_receptors_/1129225", "title"=>"Functional substitution by other OA receptors.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-08-06 03:04:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1623411"], "description"=>"<p>Ectopic expression of PKAc or CaMKII-R3 was induced with RS-GAL4 in the oviduct epithelium of the <i>octβ2r</i> mutant females. The <i>octβ2r</i> females carrying RS-GAL4 along with UAS-PKAc or UAS-CaMKII-R3 partially rescued the <i>octβ2r</i>’s ovulation (A) and fecundity (B) phenotypes (***, <i>p</i><0.0001, <i>n</i> = 21–26 for ovulation tests, <i>n</i> = 21–38 for fecundity tests).</p>", "links"=>[], "tags"=>["anatomy", "Reproductive system", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "physiology", "Reproductive physiology", "Model organisms", "Animal models", "activates", "pka", "camkii", "oviduct", "epithelium", "ovulation"], "article_id"=>1129224, "categories"=>["Biological Sciences"], "users"=>["Junghwa Lim", "Paul R. Sabandal", "Ana Fernández", "John Martin Sabandal", "Hyun-Gwan Lee", "Peter Evans", "Kyung-An Han"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104441.g004", "stats"=>{"downloads"=>5, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Oct_946_2R_activates_both_PKA_and_CaMKII_in_the_oviduct_epithelium_for_ovulation_and_fecundity_/1129224", "title"=>"Octβ2R activates both PKA and CaMKII in the oviduct epithelium for ovulation and fecundity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-08-06 03:04:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1623409"], "description"=>"<p>(A, B) Epithelial rescue. The <i>octβ2r</i> females carrying <i>RS-GAL4</i> and either <i>UAS-Octβ2R</i> or <i>UAS-Octβ2R-GFP</i> had ovulation (A) and fecundity (B) levels comparable to those of <i>CS</i> females (ns, <i>p</i>>0.05; ***, <i>p</i><0.0001; <i>n</i> = 17–24 for ovulation tests; <i>n</i> = 18–39 for fecundity tests. (C, D) Neuronal rescue. The <i>octβ2r</i> females carrying the pan-neural driver elav-GAL4 or nSyb-GAL4 along with either <i>UAS-Octβ2R</i> or <i>UAS-Octβ2R-GFP</i> exhibited ovulation (C) and fecundity (D) levels comparable to those of the <i>octβ2r</i> mutant females. ***, <i>p</i><0.0001; <i>n</i> = 12–27 for ovulation tests; <i>n</i> = 15–37 for fecundity tests. (E) Temporal rescue. The females reared at the non-permissive temperature were subjected to heat shock (+HS) to induce Octβ2R expression or kept at non-permissive temperature (−HS) as a no-induction control. The <i>octβ2r</i> females carrying <i>HS-GAL4</i> and <i>UAS-Octβ2R-GFP</i> treated with heat shock displayed ovulation levels similar to <i>CS</i> (ns, <i>p</i>>0.05; <i>n</i> = 13–30) while the females of the same genotype without heat shock showed the ovulation levels significantly different from <i>CS</i> females (***, <i>p</i><0.0001). In the graph table, “+” denotes the presence of one normal <i>octβ2r</i> allele (heterozygous <i>octβ2r</i>) or a single copy of transgenes except for the first row, which represents wild-type <i>CS</i> with two normal <i>octβ2r</i> alleles, and “−“ denotes the absence of normal <i>octβ2r</i> alleles or transgenes.</p>", "links"=>[], "tags"=>["anatomy", "Reproductive system", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "physiology", "Reproductive physiology", "Model organisms", "Animal models", "oviduct", "epithelium", "rescues", "ovulation", "fecundity"], "article_id"=>1129222, "categories"=>["Biological Sciences"], "users"=>["Junghwa Lim", "Paul R. Sabandal", "Ana Fernández", "John Martin Sabandal", "Hyun-Gwan Lee", "Peter Evans", "Kyung-An Han"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104441.g003", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Restored_Oct_2R_expression_in_the_oviduct_epithelium_rescues_the_oct_946_2r_8217_s_ovulation_and_fecundity_phenotypes_/1129222", "title"=>"Restored Octβ2R expression in the oviduct epithelium rescues the <i>octβ2r’s</i> ovulation and fecundity phenotypes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-08-06 03:04:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1623413"], "description"=>"<p>The heterozygous <i>octβ2r</i> and <i>oamb<sup>286</sup></i> mutant females having two normal alleles of <i>oamb</i> and octβ2r, respectively, and the transheterozygous <i>octβ2r/oamb<sup>286</sup></i> females were tested for ovulation (A) and fecundity (B). While the heterozygous <i>octβ2r</i> and <i>oamb<sup>286</sup></i> females showed normal ovulation and fecundity, the transheterozygous <i>octβ2r/oamb<sup>286</sup></i> females exhibited ovulation comparable to <i>CS</i> (A; ns, <i>p</i>>0.05; <i>n</i> = 20) but significantly reduced fecundity (<i>p</i><0.001; <i>n</i> = 21–22).</p>", "links"=>[], "tags"=>["anatomy", "Reproductive system", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "physiology", "Reproductive physiology", "Model organisms", "Animal models"], "article_id"=>1129226, "categories"=>["Biological Sciences"], "users"=>["Junghwa Lim", "Paul R. Sabandal", "Ana Fernández", "John Martin Sabandal", "Hyun-Gwan Lee", "Peter Evans", "Kyung-An Han"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104441.g006", "stats"=>{"downloads"=>3, "page_views"=>36, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genetic_interaction_/1129226", "title"=>"Genetic interaction.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-08-06 03:04:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1623408"], "description"=>"<p>(A) <i>CS.</i> (B) <i>octβ2r</i> carrying <i>UAS-Octβ2R-GFP</i>. (C, D) <i>octβ2r</i> carrying <i>RS-GAL4</i> and <i>UAS-Octβ2R-GFP.</i> The whole mount (A–C) and cryosectioned (D) female reproductive systems were counterstained with DAPI (blue). Octβ2R-GFP (green) expression is clearly visible in the oviduct epithelium, but not in other areas, of the <i>octβ2r</i> female carrying <i>RS-GAL4</i> and <i>UAS-Octβ2R-GFP</i> (C, D). od, oviduct; ep, oviduct epithelial layer; m, oviduct muscle layer demarcated with dashed lines. Scale bars, 100 µm.</p>", "links"=>[], "tags"=>["anatomy", "Reproductive system", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "physiology", "Reproductive physiology", "Model organisms", "Animal models", "oviduct"], "article_id"=>1129221, "categories"=>["Biological Sciences"], "users"=>["Junghwa Lim", "Paul R. Sabandal", "Ana Fernández", "John Martin Sabandal", "Hyun-Gwan Lee", "Peter Evans", "Kyung-An Han"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104441.g002", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Oct_946_2R_expression_in_the_oviduct_epithelium_/1129221", "title"=>"Octβ2R expression in the oviduct epithelium.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-08-06 03:04:00"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"20", "full-text"=>"8", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"19", "full-text"=>"20", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"18", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"40", "full-text"=>"17", "pdf"=>"14", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"27", "full-text"=>"19", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"23", "full-text"=>"15", "pdf"=>"10", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"26", "full-text"=>"17", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"22", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"14", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"7", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"12"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[267]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[280]}, {"subject_area"=>"/Biology and life sciences/Population biology", "average_usage"=>[312, 475]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences/Endocrinology", "average_usage"=>[282, 453]}, {"subject_area"=>"/Medicine and health sciences/Urology", "average_usage"=>[287, 446]}]}
Loading … Spinner
There are currently no alerts