Synthesis of Non-linear Protein Dimers through a Genetically Encoded Thiol-ene Reaction
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction", "type"=>"journal", "authors"=>[{"first_name"=>"Jessica", "last_name"=>"Torres-Kolbus", "scopus_author_id"=>"24825417200"}, {"first_name"=>"Chungjung", "last_name"=>"Chou", "scopus_author_id"=>"8771655100"}, {"first_name"=>"Jihe", "last_name"=>"Liu", "scopus_author_id"=>"56228669500"}, {"first_name"=>"Alexander", "last_name"=>"Deiters", "scopus_author_id"=>"6602272097"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"373937092", "sgr"=>"84929512070", "issn"=>"19326203", "pmid"=>"25181502", "scopus"=>"2-s2.0-84929512070", "doi"=>"10.1371/journal.pone.0105467"}, "id"=>"88fa17eb-56aa-3d73-84df-b771359bcc31", "abstract"=>"Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic encoding of a set of alkene lysines using the orthogonal PylRS/PylTCUA pair in Escherichia coli. The installed double bond functionality was then applied in a photoinitiated thiol-ene reaction of the protein with a fluorescent thiol-bearing probe, as well as a cysteine residue of a second protein, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins.", "link"=>"http://www.mendeley.com/research/synthesis-nonlinear-protein-dimers-through-genetically-encoded-thiolene-reaction", "reader_count"=>27, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>12, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>5, "Professor"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>12, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>5, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>11, "Chemistry"=>14}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Chemistry"=>{"Chemistry"=>14}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>11}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}}, "reader_count_by_country"=>{"United States"=>1, "United Kingdom"=>1}, "group_count"=>2}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84929512070"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84929512070?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84929512070&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84929512070&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84929512070", "dc:identifier"=>"SCOPUS_ID:84929512070", "eid"=>"2-s2.0-84929512070", "dc:title"=>"Synthesis of non-linear protein dimers through a genetically encoded thiol-ene reaction", "dc:creator"=>"Torres-Kolbus J.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"9", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-01-01", "prism:coverDisplayDate"=>"2014", "prism:doi"=>"10.1371/journal.pone.0105467", "citedby-count"=>"7", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"North Carolina State University", "affiliation-city"=>"Raleigh", "affiliation-country"=>"United States"}], "pubmed-id"=>"25181502", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e105467", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0105467", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Journal Comments | Further Information

Counter

  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"70", "xml_views"=>"11", "html_views"=>"247"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"2", "html_views"=>"75"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"82"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"77"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"48"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"50"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"23", "xml_views"=>"2", "html_views"=>"47"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"27", "xml_views"=>"2", "html_views"=>"54"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"44"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"20"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1658159"], "description"=>"<p>(A) sfGFP bearing an alkene functionality reacts photochemically with dansyl-thiol <b>(10)</b> or lysozyme (LYZ). (B) SDS-PAGE analysis demonstrates the labeling of alkenyl-sfGFP with <b>10</b> after 5 min of UV irradiation via thiol-ene ligation (lanes 5 and 6). Fluorescence (top) and Coomassie stain (bottom). (C) SDS-PAGE analysis shows mobility band shifts from 28 kD to 44 kD after samples were UV irradiated for 10 min (lanes 8 and 9), corresponding to the molecular weight of sfGFP-lysozyme conjugate. WT: wild-type sfGFP; <b>1</b> and <b>2</b>: sfGFP carrying the corresponding UAA; LYZ: lysozyme. –UV: samples were not exposed to UV irradiation. +UV: samples were irradiated at 365 nm for 5 or 10 min.</p>", "links"=>[], "tags"=>["probe", "bond functionality", "protein", "alkene lysines", "Escherichia coli", "cysteine residue"], "article_id"=>1158296, "categories"=>["Biological Sciences"], "users"=>["Jessica Torres-Kolbus", "Chungjung Chou", "Jihe Liu", "Alexander Deiters"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0105467.g003", "stats"=>{"downloads"=>1, "page_views"=>134, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alkenyl_sfGFP_is_fluorescently_labeled_with_dansyl_thiol_and_bioconjugated_to_lysozyme_to_assemble_a_non_linear_protein_dimer_via_the_thiol_ene_reaction_/1158296", "title"=>"Alkenyl-sfGFP is fluorescently labeled with dansyl-thiol, and bioconjugated to lysozyme to assemble a non-linear protein dimer via the thiol-ene reaction.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-02 03:39:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1658157"], "description"=>"<p>(A) Structures of alkenyl lysine derivatives bearing an ε-carbamate linkage (<b>1</b>–<b>6</b>), an inverted carbamate <b>7</b>, an amide <b>8</b>, and an urea <b>9</b>. (B) Myoglobin comparative incorporation efficiencies (%) and ESI-MS results.</p>", "links"=>[], "tags"=>["probe", "bond functionality", "protein", "alkene lysines", "Escherichia coli", "cysteine residue"], "article_id"=>1158294, "categories"=>["Biological Sciences"], "users"=>["Jessica Torres-Kolbus", "Chungjung Chou", "Jihe Liu", "Alexander Deiters"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0105467.g001", "stats"=>{"downloads"=>3, "page_views"=>40, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genetic_incorporation_of_alkene_lysine_analogs_into_myoglobin_by_the_wild_type_Mb_PylRS_PylT_CUA_pair_/1158294", "title"=>"Genetic incorporation of alkene-lysine analogs into myoglobin by the wild-type <i>Mb</i>PylRS/PylT<sub>CUA</sub> pair.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-02 03:39:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1658160", "https://ndownloader.figshare.com/files/1658161", "https://ndownloader.figshare.com/files/1658162", "https://ndownloader.figshare.com/files/1658163", "https://ndownloader.figshare.com/files/1658164", "https://ndownloader.figshare.com/files/1658165", "https://ndownloader.figshare.com/files/1658166"], "description"=>"<div><p>Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic encoding of a set of alkene lysines using the orthogonal PylRS/PylT<sub>CUA</sub> pair in <i>Escherichia coli</i>. The installed double bond functionality was then applied in a photoinitiated thiol-ene reaction of the protein with a fluorescent thiol-bearing probe, as well as a cysteine residue of a second protein, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins.</p></div>", "links"=>[], "tags"=>["probe", "bond functionality", "protein", "alkene lysines", "Escherichia coli", "cysteine residue"], "article_id"=>1158297, "categories"=>["Biological Sciences"], "users"=>["Jessica Torres-Kolbus", "Chungjung Chou", "Jihe Liu", "Alexander Deiters"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0105467.s001", "https://dx.doi.org/10.1371/journal.pone.0105467.s002", "https://dx.doi.org/10.1371/journal.pone.0105467.s003", "https://dx.doi.org/10.1371/journal.pone.0105467.s004", "https://dx.doi.org/10.1371/journal.pone.0105467.s005", "https://dx.doi.org/10.1371/journal.pone.0105467.s006", "https://dx.doi.org/10.1371/journal.pone.0105467.s007"], "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Synthesis_of_Non_linear_Protein_Dimers_through_a_Genetically_Encoded_Thiol_ene_Reaction_/1158297", "title"=>"Synthesis of Non-linear Protein Dimers through a Genetically Encoded Thiol-ene Reaction", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-09-02 03:39:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1658158"], "description"=>"<p>(A) SDS-PAGE analysis of purified sfGFP. –AA: no UAA was supplemented; WT: wild-type sfGFP; <b>1</b>, <b>2</b> and <b>3</b>: expression in the presence of the corresponding UAA (1 mM). (B) Protein yields (*wild-type sfGFP yield is 70 mg/L, 100%) and ESI-MS results.</p>", "links"=>[], "tags"=>["probe", "bond functionality", "protein", "alkene lysines", "Escherichia coli", "cysteine residue"], "article_id"=>1158295, "categories"=>["Biological Sciences"], "users"=>["Jessica Torres-Kolbus", "Chungjung Chou", "Jihe Liu", "Alexander Deiters"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0105467.g002", "stats"=>{"downloads"=>0, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genetic_incorporation_of_alkene_lysine_analogs_1_2_and_3_into_sfGFP_/1158295", "title"=>"Genetic incorporation of alkene-lysine analogs 1, 2 and 3 into sfGFP.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-02 03:39:21"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"33", "full-text"=>"23", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"25", "full-text"=>"18", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[271]}, {"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[262]}, {"subject_area"=>"/Physical sciences/Materials science", "average_usage"=>[259]}, {"subject_area"=>"/Physical sciences/Physics", "average_usage"=>[266]}]}
Loading … Spinner
There are currently no alerts