Profiling the Mitochondrial Proteome of Leber’s Hereditary Optic Neuropathy (LHON) in Thailand: Down-Regulation of Bioenergetics and Mitochondrial Protein Quality Control Pathways in Fibroblasts with the 11778G>A Mutation
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{"title"=>"Profiling the mitochondrial proteome of leber's hereditary optic neuropathy (LHON) in Thailand: Down-regulation of bioenergetics and mitochondrial protein quality control pathways in fibroblasts with the 11778G>.a mutation", "type"=>"journal", "authors"=>[{"first_name"=>"Aung Win", "last_name"=>"Tun", "scopus_author_id"=>"36973975500"}, {"first_name"=>"Sakdithep", "last_name"=>"Chaiyarit", "scopus_author_id"=>"32667508300"}, {"first_name"=>"Supannee", "last_name"=>"Kaewsutthi", "scopus_author_id"=>"49663582900"}, {"first_name"=>"Wanphen", "last_name"=>"Katanyoo", "scopus_author_id"=>"55847555600"}, {"first_name"=>"Wanicha", "last_name"=>"Chuenkongkaew", "scopus_author_id"=>"6603059773"}, {"first_name"=>"Masayoshi", "last_name"=>"Kuwano", "scopus_author_id"=>"13402976800"}, {"first_name"=>"Takeshi", "last_name"=>"Tomonaga", "scopus_author_id"=>"7003297126"}, {"first_name"=>"Chayanon", "last_name"=>"Peerapittayamongkol", "scopus_author_id"=>"25121989900"}, {"first_name"=>"Visith", "last_name"=>"Thongboonkerd", "scopus_author_id"=>"6603472979"}, {"first_name"=>"Patcharee", "last_name"=>"Lertrit", "scopus_author_id"=>"6603794975"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84933073935", "doi"=>"10.1371/journal.pone.0106779", "pui"=>"608993993", "pmid"=>"25215595", "scopus"=>"2-s2.0-84933073935", "issn"=>"19326203", "isbn"=>"1932-6203"}, "id"=>"eb70cdf5-457e-3ffe-906f-d3d9cef4ca9c", "abstract"=>"Leber's Hereditary Optic Neuropathy (LHON) is one of the commonest mitochondrial diseases. It causes total blindness, and predominantly affects young males. For the disease to develop, it is necessary for an individual to carry one of the primary mtDNA mutations 11778G>A, 14484T>C or 3460G>A. However these mutations are not sufficient to cause disease, and they do not explain the characteristic features of LHON such as the higher prevalence in males, incomplete penetrance, and relatively later age of onset. In order to explore the roles of nuclear encoded mitochondrial proteins in development of LHON, we applied a proteomic approach to samples from affected and unaffected individuals from 3 pedigrees and from 5 unrelated controls. Two-dimensional electrophoresis followed by MS/MS analysis in the mitochondrial lysate identified 17 proteins which were differentially expressed between LHON cases and unrelated controls, and 24 proteins which were differentially expressed between unaffected relatives and unrelated controls. The proteomic data were successfully validated by western blot analysis of 3 selected proteins. All of the proteins identified in the study were mitochondrial proteins and most of them were down regulated in 11778G>A mutant fibroblasts. These proteins included: subunits of OXPHOS enzyme complexes, proteins involved in intermediary metabolic processes, nucleoid related proteins, chaperones, cristae remodelling proteins and an anti-oxidant enzyme. The protein profiles of both the affected and unaffected 11778G>A carriers shared many features which differed from those of unrelated control group, revealing similar proteomic responses to 11778G>A mutation in both affected and unaffected individuals. Differentially expressed proteins revealed two broad groups: a cluster of bioenergetic pathway proteins and a cluster involved in protein quality control system. Defects in these systems are likely to impede the function of retinal ganglion cells, and may lead to the development of LHON in synergy with the primary mtDNA mutation.", "link"=>"http://www.mendeley.com/research/profiling-mitochondrial-proteome-lebers-hereditary-optic-neuropathy-lhon-thailand-downregulation-bio", "reader_count"=>23, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>2, "Researcher"=>4, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>3}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>2, "Researcher"=>4, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>3}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>5, "Agricultural and Biological Sciences"=>9, "Medicine and Dentistry"=>6, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>6}, "Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United States"=>1, "Switzerland"=>1, "Germany"=>1}, "group_count"=>2}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1674434"], "description"=>"<p>Fibroblast surface protein (FSP) was used as a marker in immunofluorescence of the cultured fibroblasts obtained directly obtained from the skin biopsy. The green represents fibroblasts and the nucleus was stained with Hoechst-dye 33342 which shows blue.</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1168982, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.g001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Assessment_of_the_purity_of_fibroblasts_from_a_cultured_skin_biopsy_/1168982", "title"=>"Assessment of the purity of fibroblasts from a cultured skin biopsy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674488"], "description"=>"<p>Equal amounts of proteins (100 µg) from each sample were resolved by 2-DE. The numbers indicate the spot IDs of proteins whose expression levels differ significantly between the LHON cases and their unaffected relatives.</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169032, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.g005", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_proteome_map_of_differentially_expressed_proteins_from_the_mitochondrial_fractions_of_fibroblasts_from_A_LHON_cases_n_8202_8202_7_and_U_unaffected_LHON_individuals_n_8202_8202_3_/1169032", "title"=>"Representative proteome map of differentially expressed proteins from the mitochondrial fractions of fibroblasts from (A) LHON cases (n = 7) and (U) unaffected LHON individuals (n = 3).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674491"], "description"=>"<p>These figures show: (A) The differentially expression of heat-shock protein 60 and catalase between LHON cases and controls; and (B) the differential expression of NDUFS1 and catalase between unaffected mutation carriers and controls. VDAC was used as a loading control. The two lanes represent samples from two different individuals for each group of samples.</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169036, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.g006", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Validation_of_proteomic_data_by_Western_Blot_analysis_/1169036", "title"=>"Validation of proteomic data by Western Blot analysis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674459"], "description"=>"<p>Equal amounts of proteins (100 µg) from each fibroblast sample were resolved by 2-DE. The numbers indicate the spot IDs of proteins whose expression levels differ significantly between the fibroblasts of LHON cases and controls.</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169004, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.g003", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_proteome_map_of_differentially_expressed_proteins_from_the_mitochondrial_fractions_of_fibroblasts_from_A_LHON_cases_n_8202_8202_7_and_C_controls_n_8202_8202_5_/1169004", "title"=>"Representative proteome map of differentially expressed proteins from the mitochondrial fractions of fibroblasts from (A) LHON cases (n = 7) and (C) controls (n = 5).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674502"], "description"=>"<p>Log-ratios of protein spot intensities were plotted according to the method of <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106779#pone.0106779-Caldas1\" target=\"_blank\">[63]</a>. In the case that one protein was identified in several spots, the only spot intensity that gave the highest significant value was selected for this plot. Negative changes were observed in all discoveries. Group A includes protein spots which were significantly differentially expressed in comparisons of affected and unaffected carriers, affected individuals and controls, and unaffected individuals and controls (Post Hoc Tukey Test; <i>P</i>-value<0.05). Group B includes protein spots which were significantly differentially expressed in comparisons of affected and unaffected carriers, and between unaffected carriers and controls. Changes between affected individuals and controls were not significant. Group C includes protein spots which were significantly differentially expressed only in the comparison between affected individuals and control. Group D includes protein spots which were significantly differentially expressed only in the comparison between unaffected carriers and controls. Group E includes protein spots which were significantly differentially expressed the comparisons between affected individuals and controls, and between unaffected carriers and controls. Here, the differences in expression between affected and unaffected carriers are not significantly different. <b>(B) Venn diagram representation of proteins identified in three comparisons derived from </b><a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106779#pone-0106779-g007\" target=\"_blank\"><b>figure 7A</b></a><b>.</b></p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169041, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.g007", "stats"=>{"downloads"=>2, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_A_plot_of_log_ratio_spot_intensities_comparing_affected_individuals_black_bars_and_unaffected_relatives_grey_bars_versus_controls_/1169041", "title"=>"(A) A plot of log-ratio spot intensities, comparing affected individuals (black bars) and unaffected relatives (grey bars) versus controls.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674503"], "description"=>"<p>*MtDNA/nuclear DNA from fibroblast of each individual was measured according to Pejznochova M, 2008 <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106779#pone.0106779-Pejznochova1\" target=\"_blank\">[62]</a> by amplification of <i>ND5</i> gene in mitochondrial genome from position 13466–13650 (GenBank sequence NC_012920 gi:251831106), and <i>PARL</i> gene from nuclear genome from 16912–17165 (GenBank sequence: NC_000003.12 gi:224589815). Real-time PCR amplification was performed on Bio-Rad CFX 96 thermo cycler and the threshold cycle (Ct) was obtained. MtDNA/nuclear DNA ratio was calculated from the Ct (mtDNA)/Ct (nDNA) ratio. The increasing ratio shows decrease in amount of mtDNA per cell <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106779#pone.0106779-Pejznochova1\" target=\"_blank\">[62]</a>.</p><p>Characteristics of LHON cases and unaffected relatives recruited in the study.</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169042, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.t001", "stats"=>{"downloads"=>10, "page_views"=>36, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characteristics_of_LHON_cases_and_unaffected_relatives_recruited_in_the_study_/1169042", "title"=>"Characteristics of LHON cases and unaffected relatives recruited in the study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674504"], "description"=>"<p>*Gene name according to HUGO Gene Nomenclature Committee.</p>#<p>indicates <i>P</i> value based on Post Hoc Tukey Test after one way ANOVA and the value less than 0.05 is considered significant in both tests.</p><p>Summary of significantly altered proteins between the LHON cases and the control group.</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169044, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.t002", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_significantly_altered_proteins_between_the_LHON_cases_and_the_control_group_/1169044", "title"=>"Summary of significantly altered proteins between the LHON cases and the control group.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674507"], "description"=>"<p>*Gene name according to HUGO Gene Nomenclature Committee.</p>#<p>indicates <i>P</i> value based on Post Hoc Tukey Test after one way ANOVA and the value less than 0.05 is considered significant in both tests.</p><p>Summary of significantly altered proteins between the unaffected LHON individuals and the control group.</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169046, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.t003", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_significantly_altered_proteins_between_the_unaffected_LHON_individuals_and_the_control_group_/1169046", "title"=>"Summary of significantly altered proteins between the unaffected LHON individuals and the control group.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674508"], "description"=>"<p>*Gene name according to HUGO Gene Nomenclature Committee.</p>#<p>indicates <i>P</i> value based on Post Hoc Tukey Test after one way ANOVA and the value less than 0.05 is considered significant in both tests.</p><p>Summary of significantly altered proteins between the LHON cases and the unaffected relatives.</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169047, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.t004", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_significantly_altered_proteins_between_the_LHON_cases_and_the_unaffected_relatives_/1169047", "title"=>"Summary of significantly altered proteins between the LHON cases and the unaffected relatives.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674510"], "description"=>"<p>M = Mitochondria, MI = Mitochondrial intermembrane space, ER = Endoplasmic Reticulum, C = Cytoplasm, N = Nucleus, CSK = cytoskeleton.</p><p>Functional categories and sub-cellular localizations of proteins identified in the study based on the MitoMiner Database and Nextprot.</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169049, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.t005", "stats"=>{"downloads"=>3, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Functional_categories_and_sub_cellular_localizations_of_proteins_identified_in_the_study_based_on_the_MitoMiner_Database_and_Nextprot_/1169049", "title"=>"Functional categories and sub-cellular localizations of proteins identified in the study based on the MitoMiner Database and Nextprot.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674511"], "description"=>"<p>Differentially expressed proteins which show similar trends of down-regulation in the mitochondrial proteomes of both LHON cases and unaffected relatives when compared with controls.</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169050, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.t006", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differentially_expressed_proteins_which_show_similar_trends_of_down_regulation_in_the_mitochondrial_proteomes_of_both_LHON_cases_and_unaffected_relatives_when_compared_with_controls_/1169050", "title"=>"Differentially expressed proteins which show similar trends of down-regulation in the mitochondrial proteomes of both LHON cases and unaffected relatives when compared with controls.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674475"], "description"=>"<p>Equal amounts of proteins (100 µg) from each sample were resolved by 2-DE. The numbers indicate the spot IDs of proteins whose expression levels differ significantly between the fibroblasts of unaffected LHON individuals and controls. Some spots in the same horizontal row showed the same proteins. For example, spot IDs 300 and 301 were identified as LONP1 and IDs 448, 451, 468 as catalase.</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169019, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.g004", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_proteome_map_of_differentially_expressed_proteins_from_mitochondria_fraction_between_fibroblasts_from_U_the_unaffected_LHON_individuals_n_8202_8202_3_and_C_the_controls_n_8202_8202_5_/1169019", "title"=>"Representative proteome map of differentially expressed proteins from mitochondria fraction between fibroblasts from (U) the unaffected LHON individuals (n = 3) and (C) the controls (n = 5).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674441"], "description"=>"<p>20 µg of mitochondrial lysate and whole cell lysate from fibroblasts were separated by 12% SDS-PAGE gel and checked with specific antibodies against various sub-cellular organelles. (W = whole cell lysate; M = mitochondrial enriched fraction) (The same membrane for each cell type was stripped and probed with subsequent antibodies.).</p>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1168989, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779.g002", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Western_blot_analyses_for_assessment_of_mitochondrial_enrichment_and_purity_/1168989", "title"=>"Western blot analyses for assessment of mitochondrial enrichment and purity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-12 02:42:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1674515"], "description"=>"<div><p>Leber’s Hereditary Optic Neuropathy (LHON) is one of the commonest mitochondrial diseases. It causes total blindness, and predominantly affects young males. For the disease to develop, it is necessary for an individual to carry one of the primary mtDNA mutations 11778G>A, 14484T>C or 3460G>A. However these mutations are not sufficient to cause disease, and they do not explain the characteristic features of LHON such as the higher prevalence in males, incomplete penetrance, and relatively later age of onset. In order to explore the roles of nuclear encoded mitochondrial proteins in development of LHON, we applied a proteomic approach to samples from affected and unaffected individuals from 3 pedigrees and from 5 unrelated controls. Two-dimensional electrophoresis followed by MS/MS analysis in the mitochondrial lysate identified 17 proteins which were differentially expressed between LHON cases and unrelated controls, and 24 proteins which were differentially expressed between unaffected relatives and unrelated controls. The proteomic data were successfully validated by western blot analysis of 3 selected proteins. All of the proteins identified in the study were mitochondrial proteins and most of them were down regulated in 11778G>A mutant fibroblasts. These proteins included: subunits of OXPHOS enzyme complexes, proteins involved in intermediary metabolic processes, nucleoid related proteins, chaperones, cristae remodelling proteins and an anti-oxidant enzyme. The protein profiles of both the affected and unaffected 11778G>A carriers shared many features which differed from those of unrelated control group, revealing similar proteomic responses to 11778G>A mutation in both affected and unaffected individuals. Differentially expressed proteins revealed two broad groups: a cluster of bioenergetic pathway proteins and a cluster involved in protein quality control system. Defects in these systems are likely to impede the function of retinal ganglion cells, and may lead to the development of LHON in synergy with the primary mtDNA mutation.</p></div>", "links"=>[], "tags"=>["OXPHOS enzyme complexes", "bioenergetic pathway proteins", "cristae remodelling proteins", "ms", "Mitochondrial Protein Quality Control Pathways", "protein quality control system", "mitochondrial proteins", "lhon"], "article_id"=>1169054, "categories"=>["Biological Sciences"], "users"=>["Aung Win Tun", "Sakdithep Chaiyarit", "Supannee Kaewsutthi", "Wanphen Katanyoo", "Wanicha Chuenkongkaew", "Masayoshi Kuwano", "Takeshi Tomonaga", "Chayanon Peerapittayamongkol", "Visith Thongboonkerd", "Patcharee Lertrit"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0106779", "stats"=>{"downloads"=>4, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Profiling_the_Mitochondrial_Proteome_of_Leber_8217_s_Hereditary_Optic_Neuropathy_LHON_in_Thailand_Down_Regulation_of_Bioenergetics_and_Mitochondrial_Protein_Quality_Control_Pathways_in_Fibroblasts_with_the_11778G_gt_A_Mutation_/1169054", "title"=>"Profiling the Mitochondrial Proteome of Leber’s Hereditary Optic Neuropathy (LHON) in Thailand: Down-Regulation of Bioenergetics and Mitochondrial Protein Quality Control Pathways in Fibroblasts with the 11778G>A Mutation", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-09-12 02:42:08"}

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Relative Metric

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