Variation in Capsidiol Sensitivity between Phytophthora infestans and Phytophthora capsici Is Consistent with Their Host Range
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{"title"=>"Variation in capsidiol sensitivity between phytophthora infestans and phytophthora capsici is consistent withtheir host range", "type"=>"journal", "authors"=>[{"first_name"=>"Artemis", "last_name"=>"Giannakopoulou", "scopus_author_id"=>"56459108000"}, {"first_name"=>"Sebastian", "last_name"=>"Schornack", "scopus_author_id"=>"6507599249"}, {"first_name"=>"Tolga O.", "last_name"=>"Bozkurt", "scopus_author_id"=>"35089975600"}, {"first_name"=>"Dave", "last_name"=>"Haart", "scopus_author_id"=>"56657448200"}, {"first_name"=>"Dae Kyun", "last_name"=>"Ro", "scopus_author_id"=>"8574607200"}, {"first_name"=>"Juan A.", "last_name"=>"Faraldos", "scopus_author_id"=>"6507578537"}, {"first_name"=>"Sophien", "last_name"=>"Kamoun", "scopus_author_id"=>"16157156400"}, {"first_name"=>"Paul E.", "last_name"=>"O'Maille", "scopus_author_id"=>"6506673915"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"25203155", "scopus"=>"2-s2.0-84929940275", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0107462", "issn"=>"19326203", "sgr"=>"84929940275", "pui"=>"608759088"}, "id"=>"9e2e2eaf-c102-3340-a297-d8b58c6f835b", "abstract"=>"Plants protect themselves against a variety of invading pathogenic organisms via sophisticated defence mechanisms. These responses include deployment of specialized antimicrobial compounds, such as phytoalexins, that rapidly accumulate at pathogen infection sites. However, the extent to which these compounds contribute to species-level resistance and their spectrum of action remain poorly understood. Capsidiol, a defense related phytoalexin, is produced by several solanaceous plants including pepper and tobacco during microbial attack. Interestingly, capsidiol differentially affects growth and germination of the oomycete pathogens Phytophthora infestans and Phytophthora capsici, although the underlying molecular mechanisms remain unknown. In this study we revisited the differential effect of capsidiol on P. infestans and P. capsici, using highly pure capsidiol preparations obtained from yeast engineered to express the capsidiol biosynthetic pathway. Taking advantage of transgenic Phytophthora strains expressing fluorescent markers, we developed a fluorescence-based method to determine the differential effect of capsidiol on Phytophtora growth. Using these assays, we confirm major differences in capsidiol sensitivity between P. infestans and P. capsici and demonstrate that capsidiol alters the growth behaviour of both Phytophthora species. Finally, we report intraspecific variation within P. infestans isolates towards capsidiol tolerance pointing to an arms race between the plant and the pathogens in deployment of defence related phytoalexins.", "link"=>"http://www.mendeley.com/research/variation-capsidiol-sensitivity-between-phytophthora-infestans-phytophthora-capsici-consistent-witht", "reader_count"=>36, "reader_count_by_academic_status"=>{"Researcher"=>6, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>1, "Student > Master"=>6, "Other"=>5, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>6, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>1, "Student > Master"=>6, "Other"=>5, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Environmental Science"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>29, "Medicine and Dentistry"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>29}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}, "Environmental Science"=>{"Environmental Science"=>2}}, "reader_count_by_country"=>{"Colombia"=>1, "United States"=>1, "Germany"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1668010"], "description"=>"<p>The experiment was performed 3 times.</p>", "links"=>[], "tags"=>["Host Range Plants", "Phytophthora capsici", "report intraspecific variation", "oomycete pathogens Phytophthora infestans", "pathogen infection sites", "capsidiol biosynthetic pathway", "phytoalexin"], "article_id"=>1165921, "categories"=>["Biological Sciences"], "users"=>["Artemis Giannakopoulou", "Sebastian Schornack", "Tolga O. Bozkurt", "Dave Haart", "Dae-kyun Ro", "Juan A. Faraldos", "Sophien Kamoun", "Paul E. O’Maille"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0107462.g006", "stats"=>{"downloads"=>1, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Growth_behaviour_of_P_infestans_88069td_after_10_days_of_exposure_to_different_capsidiol_concentrations_/1165921", "title"=>"Growth behaviour of <i>P. infestans</i> 88069td, after 10 days of exposure to different capsidiol concentrations.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-09 03:07:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1668013"], "description"=>"<p>The experiment was performed 3 times.</p>", "links"=>[], "tags"=>["Host Range Plants", "Phytophthora capsici", "report intraspecific variation", "oomycete pathogens Phytophthora infestans", "pathogen infection sites", "capsidiol biosynthetic pathway", "phytoalexin"], "article_id"=>1165924, "categories"=>["Biological Sciences"], "users"=>["Artemis Giannakopoulou", "Sebastian Schornack", "Tolga O. Bozkurt", "Dave Haart", "Dae-kyun Ro", "Juan A. Faraldos", "Sophien Kamoun", "Paul E. O’Maille"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0107462.g007", "stats"=>{"downloads"=>2, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Growth_behaviour_of_P_capsici_tdtom_after_10_days_of_exposure_to_different_capsidiol_concentrations_/1165924", "title"=>"Growth behaviour of <i>P. capsici</i> tdtom, after 10 days of exposure to different capsidiol concentrations.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-09 03:07:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1668014"], "description"=>"<p>Isolates are clustered according to their sensitivity, starting from the most sensitive to the least. Top row of wells of each isolate represents capsidiol treatment (capsidiol was dissolved in DMSO/Plich media) in µM and the lower row represents treatment with 1% (v/v) DMSO/Plich (negative control). The experiment was performed 3 times.</p>", "links"=>[], "tags"=>["Host Range Plants", "Phytophthora capsici", "report intraspecific variation", "oomycete pathogens Phytophthora infestans", "pathogen infection sites", "capsidiol biosynthetic pathway", "phytoalexin"], "article_id"=>1165925, "categories"=>["Biological Sciences"], "users"=>["Artemis Giannakopoulou", "Sebastian Schornack", "Tolga O. Bozkurt", "Dave Haart", "Dae-kyun Ro", "Juan A. Faraldos", "Sophien Kamoun", "Paul E. O’Maille"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0107462.g008", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Different_P_infestans_isolates_have_different_sensitivity_to_capsidiol_/1165925", "title"=>"Different <i>P. infestans</i> isolates have different sensitivity to capsidiol.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-09 03:07:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1668015"], "description"=>"<p>Provenance of <i>Phytophthora</i> samples.</p>", "links"=>[], "tags"=>["Host Range Plants", "Phytophthora capsici", "report intraspecific variation", "oomycete pathogens Phytophthora infestans", "pathogen infection sites", "capsidiol biosynthetic pathway", "phytoalexin"], "article_id"=>1165926, "categories"=>["Biological Sciences"], "users"=>["Artemis Giannakopoulou", "Sebastian Schornack", "Tolga O. Bozkurt", "Dave Haart", "Dae-kyun Ro", "Juan A. Faraldos", "Sophien Kamoun", "Paul E. O’Maille"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0107462.t001", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Provenance_of_Phytophthora_samples_/1165926", "title"=>"Provenance of <i>Phytophthora</i> samples.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-09-09 03:07:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1668019", "https://ndownloader.figshare.com/files/1668020"], "description"=>"<div><p>Plants protect themselves against a variety of invading pathogenic organisms via sophisticated defence mechanisms. These responses include deployment of specialized antimicrobial compounds, such as phytoalexins, that rapidly accumulate at pathogen infection sites. However, the extent to which these compounds contribute to species-level resistance and their spectrum of action remain poorly understood. Capsidiol, a defense related phytoalexin, is produced by several solanaceous plants including pepper and tobacco during microbial attack. Interestingly, capsidiol differentially affects growth and germination of the oomycete pathogens <i>Phytophthora infestans</i> and <i>Phytophthora capsici</i>, although the underlying molecular mechanisms remain unknown. In this study we revisited the differential effect of capsidiol on <i>P. infestans</i> and <i>P. capsici,</i> using highly pure capsidiol preparations obtained from yeast engineered to express the capsidiol biosynthetic pathway. Taking advantage of transgenic <i>Phytophthora</i> strains expressing fluorescent markers, we developed a fluorescence-based method to determine the differential effect of capsidiol on <i>Phytophtora</i> growth. Using these assays, we confirm major differences in capsidiol sensitivity between <i>P. infestans</i> and <i>P. capsici</i> and demonstrate that capsidiol alters the growth behaviour of both <i>Phytophthora</i> species. Finally, we report intraspecific variation within <i>P. infestans</i> isolates towards capsidiol tolerance pointing to an arms race between the plant and the pathogens in deployment of defence related phytoalexins.</p></div>", "links"=>[], "tags"=>["Host Range Plants", "Phytophthora capsici", "report intraspecific variation", "oomycete pathogens Phytophthora infestans", "pathogen infection sites", "capsidiol biosynthetic pathway", "phytoalexin"], "article_id"=>1165930, "categories"=>["Biological Sciences"], "users"=>["Artemis Giannakopoulou", "Sebastian Schornack", "Tolga O. Bozkurt", "Dave Haart", "Dae-kyun Ro", "Juan A. Faraldos", "Sophien Kamoun", "Paul E. O’Maille"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0107462.s001", "https://dx.doi.org/10.1371/journal.pone.0107462.s002"], "stats"=>{"downloads"=>3, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Variation_in_Capsidiol_Sensitivity_between_Phytophthora_infestans_and_Phytophthora_capsici_Is_Consistent_with_Their_Host_Range/1165930", "title"=>"Variation in Capsidiol Sensitivity between <i>Phytophthora infestans</i> and <i>Phytophthora capsici</i> Is Consistent with Their Host Range", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-09-09 03:07:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1668003"], "description"=>"<p>(A) Verification of capsidiol purity as tested by NMR spectroscopy (Nuclear Magnetic Resonance Spectroscopy). <sup>1</sup>H NMR (CDCl<sub>3,</sub> 600 MHz) spectrum of capsidiol. NMR integrations of the diagnostic methyl doublet at δ<sub>H</sub> 0.88 ppm (expansion) reveal a purity of greater than 98.8%. (*) Represents the impurity. (B) Growth inhibition assay of <i>P. infestans</i> and <i>P. capsici</i> after 10 days of exposure of mycelial plugs to capsidiol. Pink bar delineates the lowest concentration with an inhibitory effect and the red bar the concentration after which there is no longer growth. This experiment was performed 4 times.</p>", "links"=>[], "tags"=>["Host Range Plants", "Phytophthora capsici", "report intraspecific variation", "oomycete pathogens Phytophthora infestans", "pathogen infection sites", "capsidiol biosynthetic pathway", "phytoalexin"], "article_id"=>1165914, "categories"=>["Biological Sciences"], "users"=>["Artemis Giannakopoulou", "Sebastian Schornack", "Tolga O. Bozkurt", "Dave Haart", "Dae-kyun Ro", "Juan A. Faraldos", "Sophien Kamoun", "Paul E. O’Maille"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0107462.g001", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_P_infestans_is_more_sensitive_to_capsidiol_than_P_capsici_/1165914", "title"=>"<i>P. infestans</i> is more sensitive to capsidiol than <i>P. capsici</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-09 03:07:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1668004"], "description"=>"<p>(A) Growth inhibition assay of <i>P. infestans</i> after 10 days of exposure of mycelial plugs to capsidiol. (B) Restoration of growth after washing treatment. Green line indicates the point after which the washing treatment was applied. The experiment was performed 3 times. Picture was taken 10 days after the washing and 20 days after initial exposure to capsidiol.</p>", "links"=>[], "tags"=>["Host Range Plants", "Phytophthora capsici", "report intraspecific variation", "oomycete pathogens Phytophthora infestans", "pathogen infection sites", "capsidiol biosynthetic pathway", "phytoalexin"], "article_id"=>1165915, "categories"=>["Biological Sciences"], "users"=>["Artemis Giannakopoulou", "Sebastian Schornack", "Tolga O. Bozkurt", "Dave Haart", "Dae-kyun Ro", "Juan A. Faraldos", "Sophien Kamoun", "Paul E. O’Maille"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0107462.g002", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Capsidiol_inhibits_P_infestans_growth_reversibly_/1165915", "title"=>"Capsidiol inhibits <i>P. infestans</i> growth reversibly.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-09 03:07:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1668005"], "description"=>"<p>(A) Dose response curves of <i>P. infestans</i> 88069td calculated at 4, 7 and 10 days for both Fluorescence intensity and OD600. (B) Dose response curves of <i>P. capsici</i> tdtom calculated at 4, 7 and 10 days for both Fluorescence intensity and OD600.</p>", "links"=>[], "tags"=>["Host Range Plants", "Phytophthora capsici", "report intraspecific variation", "oomycete pathogens Phytophthora infestans", "pathogen infection sites", "capsidiol biosynthetic pathway", "phytoalexin"], "article_id"=>1165916, "categories"=>["Biological Sciences"], "users"=>["Artemis Giannakopoulou", "Sebastian Schornack", "Tolga O. Bozkurt", "Dave Haart", "Dae-kyun Ro", "Juan A. Faraldos", "Sophien Kamoun", "Paul E. O’Maille"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0107462.g003", "stats"=>{"downloads"=>2, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Capsidiol_is_not_affecting_P_capsici_growth_as_severely_as_it_does_P_infestans_/1165916", "title"=>"Capsidiol is not affecting <i>P. capsici</i> growth as severely as it does <i>P. infestans</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-09 03:07:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1668006"], "description"=>"<p>The plots illustrate fluorescence intensity of <i>P. infestans</i> 88069td (A) and <i>P. capsici</i> tdtom (B) strains over time for a maximum of 10 days. The experiment was performed 3 times.</p>", "links"=>[], "tags"=>["Host Range Plants", "Phytophthora capsici", "report intraspecific variation", "oomycete pathogens Phytophthora infestans", "pathogen infection sites", "capsidiol biosynthetic pathway", "phytoalexin"], "article_id"=>1165917, "categories"=>["Biological Sciences"], "users"=>["Artemis Giannakopoulou", "Sebastian Schornack", "Tolga O. Bozkurt", "Dave Haart", "Dae-kyun Ro", "Juan A. Faraldos", "Sophien Kamoun", "Paul E. O’Maille"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0107462.g004", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Scatter_plots_correlating_fluorescence_intensity_and_capsidiol_concentration_/1165917", "title"=>"Scatter plots correlating fluorescence intensity and capsidiol concentration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-09 03:07:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1668007"], "description"=>"<p>The plots illustrate growth of <i>P. infestans</i> 88069td (A) and <i>P. capsici</i> tdtom (B) strains over time for a maximum of 10 days. The experiment was performed 3 times.</p>", "links"=>[], "tags"=>["Host Range Plants", "Phytophthora capsici", "report intraspecific variation", "oomycete pathogens Phytophthora infestans", "pathogen infection sites", "capsidiol biosynthetic pathway", "phytoalexin"], "article_id"=>1165918, "categories"=>["Biological Sciences"], "users"=>["Artemis Giannakopoulou", "Sebastian Schornack", "Tolga O. Bozkurt", "Dave Haart", "Dae-kyun Ro", "Juan A. Faraldos", "Sophien Kamoun", "Paul E. O’Maille"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0107462.g005", "stats"=>{"downloads"=>4, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Scatter_plots_correlating_0D600_and_capsidiol_concentration_/1165918", "title"=>"Scatter plots correlating 0D600 and capsidiol concentration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-09-09 03:07:35"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Agriculture", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[310]}]}
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