Effects of Cell-Attachment and Extracellular Matrix on Bone Formation In Vivo in Collagen-Hydroxyapatite Scaffolds
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Effects of cell-attachment and extracellular matrix on bone formation In Vivo in collagen-hydroxyapatite scaffolds", "type"=>"journal", "authors"=>[{"first_name"=>"Max M.", "last_name"=>"Villa", "scopus_author_id"=>"55886897200"}, {"first_name"=>"Liping", "last_name"=>"Wang", "scopus_author_id"=>"57071973200"}, {"first_name"=>"David W.", "last_name"=>"Rowe", "scopus_author_id"=>"26540154300"}, {"first_name"=>"Mei", "last_name"=>"Wei", "scopus_author_id"=>"7202183671"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"600175541", "sgr"=>"84908032022", "pmid"=>"25329879", "scopus"=>"2-s2.0-84908032022", "isbn"=>"1932-6203", "doi"=>"10.1371/journal.pone.0109568", "issn"=>"19326203"}, "id"=>"c7ee877b-3c51-3e41-8511-072ad222bcb0", "abstract"=>"Cell-based tissue engineering can be used to replace missing or damaged bone, but the optimal methods for delivering therapeutic cells to a bony defect have not yet been established. Using transgenic reporter cells as a donor source, two different collagen-hydroxyapatite (HA) scaffolds, and a critical-size calvarial defect model, we investigated the effect of a cell-attachment period prior to implantation, with or without an extracellular matrix-based seeding suspension, on cell engraftment and osteogenesis. When quantitatively compared, the in-house scaffold implanted immediately had a higher mean radiopacity than in-house scaffolds incubated overnight. Both scaffold types implanted immediately had significantly higher area fractions of donor cells, while the in-house collagen-HA scaffolds implanted immediately had higher area fractions of the mineralization label compared with groups incubated overnight. When the cell loading was compared in vitro for each delivery method using the in-house scaffold, immediate loading led to higher numbers of delivered cells. Immediate loading may be preferable in order to ensure robust bone formation in vivo. The use of a secondary ECM carrier improved the distribution of donor cells only when a pre-attachment period was applied. These results have improved our understanding of cell delivery to bony defects in the context of in vivo outcomes.", "link"=>"http://www.mendeley.com/research/effects-cellattachment-extracellular-matrix-bone-formation-vivo-collagenhydroxyapatite-scaffolds", "reader_count"=>24, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>3, "Researcher"=>4, "Student > Ph. D. Student"=>6, "Student > Master"=>7, "Other"=>2, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>3, "Researcher"=>4, "Student > Ph. D. Student"=>6, "Student > Master"=>7, "Other"=>2, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>3, "Biochemistry, Genetics and Molecular Biology"=>2, "Materials Science"=>5, "Medicine and Dentistry"=>4, "Agricultural and Biological Sciences"=>7, "Pharmacology, Toxicology and Pharmaceutical Science"=>2, "Linguistics"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>3}, "Materials Science"=>{"Materials Science"=>5}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Linguistics"=>{"Linguistics"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>2}}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84908032022"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84908032022?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84908032022&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84908032022&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84908032022", "dc:identifier"=>"SCOPUS_ID:84908032022", "eid"=>"2-s2.0-84908032022", "dc:title"=>"Effects of cell-attachment and extracellular matrix on bone formation In Vivo in collagen-hydroxyapatite scaffolds", "dc:creator"=>"Villa M.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"10", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-10-16", "prism:coverDisplayDate"=>"16 October 2014", "prism:doi"=>"10.1371/journal.pone.0109568", "citedby-count"=>"12", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Connecticut", "affiliation-city"=>"Storrs", "affiliation-country"=>"United States"}], "pubmed-id"=>"25329879", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e109568", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0109568", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"127", "xml_views"=>"20", "html_views"=>"449"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"44", "xml_views"=>"3", "html_views"=>"136"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"2", "html_views"=>"84"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"2", "html_views"=>"64"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"50"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"49"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"26"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"1"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1720498"], "description"=>"<p>(A) Comparison of donor cell distribution from the outside edge of the implant for Healos and Col-HA. Error bars indicate one standard deviation. Plot above shows a comparison of the means for each sample according to scaffold type. (B) Comparison of the mean donor cell distance from the outside edge of the implant. Light red bars indicate 95% confidence intervals and blue bars indicate one standard deviation.</p>", "links"=>[], "tags"=>["ecm", "defect", "donor cells", "ha", "loading", "area fractions", "scaffold"], "article_id"=>1206929, "categories"=>["Biological Sciences"], "users"=>["Max M. Villa", "Liping Wang", "David W. Rowe", "Mei Wei"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109568.g006", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Donor_cell_distribution_in_the_scaffolds_/1206929", "title"=>"Donor cell distribution in the scaffolds.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-16 03:50:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1720476"], "description"=>"<p>(A) Schematic of Experimental Design. Osteoprogenitors from the bone marrow were expanded <i>in vitro</i> before seeding to collagen-hydroxyapatite scaffolds and implanted in critical size calvarial defects. To label areas of active mineralization, calcein was injected intraperitoneally one-day prior to euthanization at three weeks post-implantation. (B) Electron micrograph of the Col-HA scaffold showing cellular morphology. Scale bar is 500 µm. (C) Enlarged inset from (A). Scale bar is 100 µm. (D) Electron micrograph of Healos scaffold. Scale bar is 500 µm. (E) Enlarged inset from (D). Scale bar is 100 µm.</p>", "links"=>[], "tags"=>["ecm", "defect", "donor cells", "ha", "loading", "area fractions", "scaffold"], "article_id"=>1206918, "categories"=>["Biological Sciences"], "users"=>["Max M. Villa", "Liping Wang", "David W. Rowe", "Mei Wei"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109568.g001", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Experimental_design_and_scaffold_morphology_/1206918", "title"=>"Experimental design and scaffold morphology.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-16 03:50:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1720477"], "description"=>"<p>(A) Phase contrast showing near confluent cell culture, (B) TdTomato reporter driven by OsterixCRE and (C) Merged image of (A) and (B) indicating a large fraction of cultured cells are osteoprogenitors.</p>", "links"=>[], "tags"=>["ecm", "defect", "donor cells", "ha", "loading", "area fractions", "scaffold"], "article_id"=>1206919, "categories"=>["Biological Sciences"], "users"=>["Max M. Villa", "Liping Wang", "David W. Rowe", "Mei Wei"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109568.g002", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Osteoprogenitor_cells_following_six_days_of_in_vitro_expansion_/1206919", "title"=>"Osteoprogenitor cells following six days of <i>in vitro</i> expansion.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-16 03:50:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1720478"], "description"=>"<p>(A)–(M) H and C denote Healos and Col-HA scaffolds, respectively. Scale bars are 1 mm. (N) Quantitation of radiographs. Light red bars indicate 95% confidence intervals and blue bars indicate one standard deviation.</p>", "links"=>[], "tags"=>["ecm", "defect", "donor cells", "ha", "loading", "area fractions", "scaffold"], "article_id"=>1206920, "categories"=>["Biological Sciences"], "users"=>["Max M. Villa", "Liping Wang", "David W. Rowe", "Mei Wei"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109568.g003", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Radiographs_of_cell_scaffold_constructs_after_3_weeks_in_vivo_/1206920", "title"=>"Radiographs of cell-scaffold constructs after 3 weeks <i>in vivo</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-16 03:50:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1720524", "https://ndownloader.figshare.com/files/1720525", "https://ndownloader.figshare.com/files/1720526"], "description"=>"<div><p>Cell-based tissue engineering can be used to replace missing or damaged bone, but the optimal methods for delivering therapeutic cells to a bony defect have not yet been established. Using transgenic reporter cells as a donor source, two different collagen-hydroxyapatite (HA) scaffolds, and a critical-size calvarial defect model, we investigated the effect of a cell-attachment period prior to implantation, with or without an extracellular matrix-based seeding suspension, on cell engraftment and osteogenesis. When quantitatively compared, the in-house scaffold implanted immediately had a higher mean radiopacity than in-house scaffolds incubated overnight. Both scaffold types implanted immediately had significantly higher area fractions of donor cells, while the in-house collagen-HA scaffolds implanted immediately had higher area fractions of the mineralization label compared with groups incubated overnight. When the cell loading was compared <i>in vitro</i> for each delivery method using the in-house scaffold, immediate loading led to higher numbers of delivered cells. Immediate loading may be preferable in order to ensure robust bone formation <i>in vivo</i>. The use of a secondary ECM carrier improved the distribution of donor cells only when a pre-attachment period was applied. These results have improved our understanding of cell delivery to bony defects in the context of <i>in vivo</i> outcomes.</p></div>", "links"=>[], "tags"=>["ecm", "defect", "donor cells", "ha", "loading", "area fractions", "scaffold"], "article_id"=>1206955, "categories"=>["Biological Sciences"], "users"=>["Max M. Villa", "Liping Wang", "David W. Rowe", "Mei Wei"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0109568.s001", "https://dx.doi.org/10.1371/journal.pone.0109568.s002", "https://dx.doi.org/10.1371/journal.pone.0109568.s003"], "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Effects_of_Cell_Attachment_and_Extracellular_Matrix_on_Bone_Formation_In_Vivo_in_Collagen_Hydroxyapatite_Scaffolds/1206955", "title"=>"Effects of Cell-Attachment and Extracellular Matrix on Bone Formation <i>In Vivo</i> in Collagen-Hydroxyapatite Scaffolds", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-10-16 03:50:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1720493"], "description"=>"<p>(Left column) Defect wide view of scaffolds and mineralized tissue (darkfield channel), +TdTomato donor cells (red), and the mineralization label (green, calcein). Scale bars are 500 µm. (Right column) Magnifications of left column. Blue arrows indicate +TdTomato donor cells embedded in bone. White arrows indicate +TdTomato donor cells overlying mineral label. Scale bars are 100 µm.</p>", "links"=>[], "tags"=>["ecm", "defect", "donor cells", "ha", "loading", "area fractions", "scaffold"], "article_id"=>1206924, "categories"=>["Biological Sciences"], "users"=>["Max M. Villa", "Liping Wang", "David W. Rowe", "Mei Wei"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109568.g004", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Histological_sections_of_defects_following_3_weeks_of_in_vivo_implantation_/1206924", "title"=>"Histological sections of defects following 3 weeks of <i>in vivo</i> implantation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-16 03:50:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1720494"], "description"=>"<p>(A) Quantification of the darkfield area fraction in the defect area. (B) Quantification of donor cell area fraction using TdTomato signal. (C) Quantification of mineralizing surface using the calcein mineralization label. Light red bars indicate 95% confidence intervals and blue bars indicate one standard deviation.</p>", "links"=>[], "tags"=>["ecm", "defect", "donor cells", "ha", "loading", "area fractions", "scaffold"], "article_id"=>1206926, "categories"=>["Biological Sciences"], "users"=>["Max M. Villa", "Liping Wang", "David W. Rowe", "Mei Wei"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109568.g005", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Quantitative_histomorphometry_of_darkfield_donor_cell_and_calcein_channels_/1206926", "title"=>"Quantitative histomorphometry of darkfield, donor cell, and calcein channels.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-16 03:50:43"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"20", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"23", "full-text"=>"25", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"36", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"43", "full-text"=>"38", "pdf"=>"27", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"25", "full-text"=>"21", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"19", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"22", "full-text"=>"18", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"28", "full-text"=>"8", "pdf"=>"26", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"15", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"13", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[266]}, {"subject_area"=>"/Medicine and health sciences/Physiology", "average_usage"=>[278]}]}
Loading … Spinner
There are currently no alerts