Genetic Inactivation of European Sea Bass (Dicentrarchus labrax L.) Eggs Using UV-Irradiation: Observations and Perspectives
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{"title"=>"Genetic inactivation of European Sea Bass (Dicentrarchus labraxL.) Eggs using UV-irradiation: Observations and perspectives", "type"=>"journal", "authors"=>[{"first_name"=>"Julie", "last_name"=>"Colléter", "scopus_author_id"=>"56394577300"}, {"first_name"=>"David J.", "last_name"=>"Penman", "scopus_author_id"=>"7006653796"}, {"first_name"=>"Stéphane", "last_name"=>"Lallement", "scopus_author_id"=>"56394545300"}, {"first_name"=>"Christian", "last_name"=>"Fauvel", "scopus_author_id"=>"6603710932"}, {"first_name"=>"Tanja", "last_name"=>"Hanebrekke", "scopus_author_id"=>"36484582700"}, {"first_name"=>"Renate D.", "last_name"=>"Osvik", "scopus_author_id"=>"56394626900"}, {"first_name"=>"Hans C.", "last_name"=>"Eilertsen", "scopus_author_id"=>"6603608876"}, {"first_name"=>"Helena", "last_name"=>"D'Cotta", "scopus_author_id"=>"6506252858"}, {"first_name"=>"Béatrice", "last_name"=>"Chatain", "scopus_author_id"=>"6602088567"}, {"first_name"=>"Stefano", "last_name"=>"Peruzzi", "scopus_author_id"=>"6602144901"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84908179515", "sgr"=>"84908179515", "issn"=>"19326203", "arxiv"=>"arXiv:1011.1669v3", "doi"=>"10.1371/journal.pone.0109572", "pmid"=>"25329931", "isbn"=>"9788578110796", "pui"=>"600198804"}, "id"=>"235ab21a-e887-3142-b8f4-d085812ffeee", "abstract"=>"<p>Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (γ), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (<italic>Dicentrarchus labrax</italic> L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities. Despite the wide range of UV doses tested (from 7.2 to 720 mJ.cm<sup>−2</sup>), only one dose (60 mJ.cm<sup>−2</sup>.min<sup>−1</sup> with 1 min irradiation) resulted in a small percentage (14%) of haploid larvae at hatching in the initial trials as verified by flow cytometry. Microsatellite marker analyses of three further batches of larvae produced by using this UV treatment showed a majority of larvae with variable levels of paternal and maternal contributions and only one larva displaying pure paternal inheritance. The results are discussed also in the context of an assessment of the UV-absorbance characteristics of egg extracts in this species that revealed the presence of gadusol, a compound structurally related to mycosporine-like amino acids (MAAs) with known UV-screening properties.</p>", "link"=>"http://www.mendeley.com/research/genetic-inactivation-european-sea-bass-dicentrarchus-labraxl-eggs-using-uvirradiation-observations-p", "reader_count"=>13, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Lecturer > Senior Lecturer"=>1, "Professor"=>1, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Lecturer > Senior Lecturer"=>1, "Professor"=>1, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>6, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>4}, "Environmental Science"=>{"Environmental Science"=>1}}, "reader_count_by_country"=>{"Namibia"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1724486"], "description"=>"<p>Error bars represent standard deviations of means (STD).</p>", "links"=>[], "tags"=>["Microsatellite marker analyses", "Dicentrarchu", "maa", "UV irradiation", "European Sea Bass", "embryo survival", "UV treatments", "uniparental reproduction", "egg extracts", "1 min irradiation", "haploid larvae", "labrax", "Genetic Inactivation", "haploid androgenesis", "Perspectives Androgenesis", "species", "mJ.cm", "UV treatment", "UV irradiation levels", "flow cytometry", "UV doses"], "article_id"=>1210075, "categories"=>["Biological Sciences"], "users"=>["Julie Colléter", "David J. Penman", "Stéphane Lallement", "Christian Fauvel", "Tanja Hanebrekke", "Renate D. Osvik", "Hans C. Eilertsen", "Helena D’Cotta", "Béatrice Chatain", "Stefano Peruzzi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109572.g002", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Percent_survival_relative_to_controls_of_treated_groups_exposed_to_different_cumulative_UV_doses_in_the_range_7_2_720_mJ_cm_8722_2_/1210075", "title"=>"Percent survival relative to controls of treated groups exposed to different cumulative UV-doses in the range 7.2–720 mJ.cm<sup>−2</sup>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-20 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1724485"], "description"=>"<p>Error bars represent standard deviations of means (STD).</p>", "links"=>[], "tags"=>["Microsatellite marker analyses", "Dicentrarchu", "maa", "UV irradiation", "European Sea Bass", "embryo survival", "UV treatments", "uniparental reproduction", "egg extracts", "1 min irradiation", "haploid larvae", "labrax", "Genetic Inactivation", "haploid androgenesis", "Perspectives Androgenesis", "species", "mJ.cm", "UV treatment", "UV irradiation levels", "flow cytometry", "UV doses"], "article_id"=>1210074, "categories"=>["Biological Sciences"], "users"=>["Julie Colléter", "David J. Penman", "Stéphane Lallement", "Christian Fauvel", "Tanja Hanebrekke", "Renate D. Osvik", "Hans C. Eilertsen", "Helena D’Cotta", "Béatrice Chatain", "Stefano Peruzzi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109572.g001", "stats"=>{"downloads"=>1, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Percent_survival_relative_to_controls_of_hatched_larvae_issued_from_the_different_UV_irradiation_treatments_7_2_72_mJ_cm_8722_2_min_8722_1_lasting_0_5_8211_12_min_/1210074", "title"=>"Percent survival relative to controls of hatched larvae issued from the different UV-irradiation treatments (7.2–72 mJ.cm<sup>−2</sup>.min<sup>−1</sup>) lasting 0.5–12 min.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-20 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1724494"], "description"=>"<p>Genotypes of progenies showing only discriminant paternal alleles are presented in bold characters. For putative androgenetic progenies, homozygous or haploid alleles are only written once since genotyping cannot distinguish between the presence of one or two copies of the same allele. N represents the number of analyzed individuals in each progeny.</p><p>Microsatellite marker loci transmission in three putative androgenetic progenies (A1, B1 and C2) produced with a UV-dose of 60 mJ.cm<sup>−2</sup>.</p>", "links"=>[], "tags"=>["Microsatellite marker analyses", "Dicentrarchu", "maa", "UV irradiation", "European Sea Bass", "embryo survival", "UV treatments", "uniparental reproduction", "egg extracts", "1 min irradiation", "haploid larvae", "labrax", "Genetic Inactivation", "haploid androgenesis", "Perspectives Androgenesis", "species", "mJ.cm", "UV treatment", "UV irradiation levels", "flow cytometry", "UV doses"], "article_id"=>1210083, "categories"=>["Biological Sciences"], "users"=>["Julie Colléter", "David J. Penman", "Stéphane Lallement", "Christian Fauvel", "Tanja Hanebrekke", "Renate D. Osvik", "Hans C. Eilertsen", "Helena D’Cotta", "Béatrice Chatain", "Stefano Peruzzi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109572.t001", "stats"=>{"downloads"=>5, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Microsatellite_marker_loci_transmission_in_three_putative_androgenetic_progenies_A1_B1_and_C2_produced_with_a_UV_dose_of_60_mJ_cm_8722_2_/1210083", "title"=>"Microsatellite marker loci transmission in three putative androgenetic progenies (A1, B1 and C2) produced with a UV-dose of 60 mJ.cm<sup>−2</sup>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-10-20 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1724493"], "description"=>"<p>Wavelengths cover almost the entire UV spectrum (UVC: 200–280 nm, UVB: 280–315 nm, and UVA: 315–400 nm). Absorption is shown as arbitrary units.</p>", "links"=>[], "tags"=>["Microsatellite marker analyses", "Dicentrarchu", "maa", "UV irradiation", "European Sea Bass", "embryo survival", "UV treatments", "uniparental reproduction", "egg extracts", "1 min irradiation", "haploid larvae", "labrax", "Genetic Inactivation", "haploid androgenesis", "Perspectives Androgenesis", "species", "mJ.cm", "UV treatment", "UV irradiation levels", "flow cytometry", "UV doses"], "article_id"=>1210082, "categories"=>["Biological Sciences"], "users"=>["Julie Colléter", "David J. Penman", "Stéphane Lallement", "Christian Fauvel", "Tanja Hanebrekke", "Renate D. Osvik", "Hans C. Eilertsen", "Helena D’Cotta", "Béatrice Chatain", "Stefano Peruzzi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109572.g005", "stats"=>{"downloads"=>5, "page_views"=>45, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Absorbance_spectrum_of_non_irradiated_control_egg_extracts_at_pH8_dotted_line_and_pH3_solid_line_/1210082", "title"=>"Absorbance spectrum of non-irradiated (control) egg extracts at pH8 (dotted line) and pH3 (solid line).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-20 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1724488"], "description"=>"<p>a) Control diploid (2n) larva (CV: 5%); b) haploid (n) larva produced with a UV-dose of 60 mJ.cm<sup>−2</sup> (CV: 10%). DNA values on the X-axis are reported in arbitrary units expressed as fluorescent channel numbers (PE-A). G2 represent mitotic peaks.</p>", "links"=>[], "tags"=>["Microsatellite marker analyses", "Dicentrarchu", "maa", "UV irradiation", "European Sea Bass", "embryo survival", "UV treatments", "uniparental reproduction", "egg extracts", "1 min irradiation", "haploid larvae", "labrax", "Genetic Inactivation", "haploid androgenesis", "Perspectives Androgenesis", "species", "mJ.cm", "UV treatment", "UV irradiation levels", "flow cytometry", "UV doses"], "article_id"=>1210077, "categories"=>["Biological Sciences"], "users"=>["Julie Colléter", "David J. Penman", "Stéphane Lallement", "Christian Fauvel", "Tanja Hanebrekke", "Renate D. Osvik", "Hans C. Eilertsen", "Helena D’Cotta", "Béatrice Chatain", "Stefano Peruzzi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109572.g003", "stats"=>{"downloads"=>0, "page_views"=>35, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_examples_of_flow_cytometry_histograms_obtained_from_nuclear_suspensions_5_8211_10_000_counts_of_Propidium_Iodide_PI_stained_larval_samples_/1210077", "title"=>"Representative examples of flow-cytometry histograms obtained from nuclear suspensions (5–10,000 counts) of Propidium Iodide (PI) stained larval samples.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-20 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1724505", "https://ndownloader.figshare.com/files/1724506", "https://ndownloader.figshare.com/files/1724508", "https://ndownloader.figshare.com/files/1724510", "https://ndownloader.figshare.com/files/1724512", "https://ndownloader.figshare.com/files/1724514"], "description"=>"<div><p>Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (γ), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (<i>Dicentrarchus labrax</i> L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities. Despite the wide range of UV doses tested (from 7.2 to 720 mJ.cm<sup>−2</sup>), only one dose (60 mJ.cm<sup>−2</sup>.min<sup>−1</sup> with 1 min irradiation) resulted in a small percentage (14%) of haploid larvae at hatching in the initial trials as verified by flow cytometry. Microsatellite marker analyses of three further batches of larvae produced by using this UV treatment showed a majority of larvae with variable levels of paternal and maternal contributions and only one larva displaying pure paternal inheritance. The results are discussed also in the context of an assessment of the UV-absorbance characteristics of egg extracts in this species that revealed the presence of gadusol, a compound structurally related to mycosporine-like amino acids (MAAs) with known UV-screening properties.</p></div>", "links"=>[], "tags"=>["Microsatellite marker analyses", "Dicentrarchu", "maa", "UV irradiation", "European Sea Bass", "embryo survival", "UV treatments", "uniparental reproduction", "egg extracts", "1 min irradiation", "haploid larvae", "labrax", "Genetic Inactivation", "haploid androgenesis", "Perspectives Androgenesis", "species", "mJ.cm", "UV treatment", "UV irradiation levels", "flow cytometry", "UV doses"], "article_id"=>1210090, "categories"=>["Biological Sciences"], "users"=>["Julie Colléter", "David J. Penman", "Stéphane Lallement", "Christian Fauvel", "Tanja Hanebrekke", "Renate D. Osvik", "Hans C. Eilertsen", "Helena D’Cotta", "Béatrice Chatain", "Stefano Peruzzi"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0109572.s001", "https://dx.doi.org/10.1371/journal.pone.0109572.s002", "https://dx.doi.org/10.1371/journal.pone.0109572.s003", "https://dx.doi.org/10.1371/journal.pone.0109572.s004", "https://dx.doi.org/10.1371/journal.pone.0109572.s005", "https://dx.doi.org/10.1371/journal.pone.0109572.s006"], "stats"=>{"downloads"=>15, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Genetic_Inactivation_of_European_Sea_Bass_Dicentrarchus_labrax_L_Eggs_Using_UV_Irradiation_Observations_and_Perspectives/1210090", "title"=>"Genetic Inactivation of European Sea Bass (<i>Dicentrarchus labrax</i> L.) Eggs Using UV-Irradiation: Observations and Perspectives", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-10-20 02:51:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1724492"], "description"=>"<p>a) Control embryo at 74 h pf; b) control larva at hatching; c) UV treated embryos at 74 hpf showing microcephaly, short and large body; d) UV-treated larvae at hatching showing microphtalmy, short body and curved tail. Scale bars represent 500 µm.</p>", "links"=>[], "tags"=>["Microsatellite marker analyses", "Dicentrarchu", "maa", "UV irradiation", "European Sea Bass", "embryo survival", "UV treatments", "uniparental reproduction", "egg extracts", "1 min irradiation", "haploid larvae", "labrax", "Genetic Inactivation", "haploid androgenesis", "Perspectives Androgenesis", "species", "mJ.cm", "UV treatment", "UV irradiation levels", "flow cytometry", "UV doses"], "article_id"=>1210081, "categories"=>["Biological Sciences"], "users"=>["Julie Colléter", "David J. Penman", "Stéphane Lallement", "Christian Fauvel", "Tanja Hanebrekke", "Renate D. Osvik", "Hans C. Eilertsen", "Helena D’Cotta", "Béatrice Chatain", "Stefano Peruzzi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0109572.g004", "stats"=>{"downloads"=>1, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Morphology_of_control_and_UV_treated_embryos_and_larvae_/1210081", "title"=>"Morphology of control and UV-treated embryos and larvae.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-20 02:51:01"}

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