Comparative Proteomics Analysis of Gastric Cancer Stem Cells
Publication Date
November 07, 2014
Journal
PLOS ONE
Authors
Tamami Morisaki, Masakazu Yashiro, Anna Kakehashi, Azusa Inagaki, et al
Volume
9
Issue
11
Pages
e110736
DOI
https://dx.plos.org/10.1371/journal.pone.0110736
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0110736
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/25379943
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4224387
Europe PMC
http://europepmc.org/abstract/MED/25379943
Web of Science
000344863100014
Scopus
84917689681
Mendeley
http://www.mendeley.com/research/comparative-proteomics-analysis-gastric-cancer-stem-cells
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Comparative Proteomics Analysis of Gastric Cancer Stem Cells", "type"=>"journal", "authors"=>[{"first_name"=>"Tamami", "last_name"=>"Morisaki"}, {"first_name"=>"Masakazu", "last_name"=>"Yashiro"}, {"first_name"=>"Anna", "last_name"=>"Kakehashi"}, {"first_name"=>"Azusa", "last_name"=>"Inagaki"}, {"first_name"=>"Haruhito", "last_name"=>"Kinoshita"}, {"first_name"=>"Tatsunari", "last_name"=>"Fukuoka"}, {"first_name"=>"Hiroaki", "last_name"=>"Kasashima"}, {"first_name"=>"Go", "last_name"=>"Masuda"}, {"first_name"=>"Katsunobu", "last_name"=>"Sakurai"}, {"first_name"=>"Naoshi", "last_name"=>"Kubo"}, {"first_name"=>"Kazuya", "last_name"=>"Muguruma"}, {"first_name"=>"Masaichi", "last_name"=>"Ohira"}, {"first_name"=>"Hideki", "last_name"=>"Wanibuchi"}, {"first_name"=>"Kosei", "last_name"=>"Hirakawa"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"1932-6203", "pmid"=>"25379943", "doi"=>"10.1371/journal.pone.0110736"}, "id"=>"59feffb9-93de-31e6-9066-d2566bdfb3c2", "abstract"=>"Cancer stem cells (CSCs) are responsible for cancer progression, metastasis, and recurrence. To date, the specific markers of CSCs remain undiscovered. The aim of this study was to identify novel biomarkers of gastric CSCs for clinical diagnosis using proteomics technology. CSC-like SP cells, OCUM-12/SP cells, OCUM-2MD3/SP cells, and their parent OCUM-12 cells and OCUM-2MD3 cells were used in this study. Protein lysates from each cell line were analyzed using QSTAR Elite Liquid Chromatography with Tandem Mass Spectrometry, coupled with isobaric tags for relative and absolute quantitation technology. Candidate proteins detected by proteomics technology were validated by immunohistochemical analysis of 300 gastric cancers. Based on the results of LC-MS/MS, eight proteins, including RBBP6, GLG1, VPS13A, DCTPP1, HSPA9, HSPA4, ALDOA, and KRT18, were up-regulated in both OCUM-12/SP cells and OCUM-2MD3/SP cells when compared to their corresponding parent cells. RT-PCR analysis indicated that the expression level of RBBP6, HSPA4, DCTPP1, HSPA9, VPS13A, ALDOA, GLG1, and CK18 was high in OCUM-12/SP and OCUM-2MD3/SP, in compared with the control of parent OCUM-12 and OCUM-2MD3. These proteins were significantly associated with advanced invasion depth, lymph node metastasis, distant metastasis, or advanced clinical stage. RBBP6, DCTPP1, HSPA4, and ALDOA expression in particular were significantly associated with a poor prognosis in the 300 gastric cancer patients. RBBP6 was determined to be an independent prognostic factor. The motility-stimulating ability of OCUM-12/SP cells and OCUM-2MD3/SP cells was inhibited by RBBP6 siRNA. These findings might suggest that the eight proteins, RBBP6, GLG1, VPS13A, DCTPP1, HSPA9, HSPA4, ALDOA, and KRT18, utilizing comparative proteomics analysis, were perceived to be potential CSC markers of gastric cancer. Of the eight candidate proteins, RBBP6 was suggested to be a promising prognostic biomarker and a therapeutic target for gastric cancer.", "link"=>"http://www.mendeley.com/research/comparative-proteomics-analysis-gastric-cancer-stem-cells", "reader_count"=>56, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>17, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>3, "Student > Master"=>5, "Other"=>17, "Student > Bachelor"=>2, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>17, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>3, "Student > Master"=>5, "Other"=>17, "Student > Bachelor"=>2, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>12, "Agricultural and Biological Sciences"=>36, "Medicine and Dentistry"=>1, "Neuroscience"=>3, "Chemistry"=>2, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>3}, "Chemistry"=>{"Chemistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>36}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>12}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84917689681"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84917689681?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84917689681&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84917689681&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84917689681", "dc:identifier"=>"SCOPUS_ID:84917689681", "eid"=>"2-s2.0-84917689681", "dc:title"=>"Comparative proteomics analysis of gastric cancer stem cells", "dc:creator"=>"Morisaki T.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"11", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-11-07", "prism:coverDisplayDate"=>"7 November 2014", "prism:doi"=>"10.1371/journal.pone.0110736", "citedby-count"=>"23", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Osaka City University", "affiliation-city"=>"Osaka", "affiliation-country"=>"Japan"}], "pubmed-id"=>"25379943", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e110736", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0110736", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"134", "xml_views"=>"15", "html_views"=>"421"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"40", "xml_views"=>"4", "html_views"=>"128"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"19", "xml_views"=>"2", "html_views"=>"76"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"78"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"28"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"40"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"29"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"39"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"8"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1783704"], "description"=>"a<p>NF; Infiltration pattern of tumor. The predominant pattern of infiltrating growth into the surrounding tissue is classified as follows; INF a: The tumor shows expanding growth and a distinct border with the surrounding tissue. INF b: This category is between INF a and INF b. INF c: The tumor shows infiltrating growth and an indistinct border with the surrounding tissue.</p><p>Univariate and multivariate analyses with respect to survival.</p>", "links"=>[], "tags"=>["RBBP 6 siRNA", "proteomics technology", "13a", "Tandem mass spectrometry", "hspa", "Comparative Proteomics Analysis", "DCTPP", "RBBP 6", "csc", "GLG", "Gastric Cancer Stem Cells Cancer", "sp", "lymph node metastasis", "ck", "protein", "QSTAR Elite Liquid Chromatography", "OCUM", "krt", "aldoa", "vps"], "article_id"=>1232429, "categories"=>["Uncategorised"], "users"=>["Tamami Morisaki", "Masakazu Yashiro", "Anna Kakehashi", "Azusa Inagaki", "Haruhito Kinoshita", "Tatsunari Fukuoka", "Hiroaki Kasashima", "Go Masuda", "Katsunobu Sakurai", "Naoshi Kubo", "Kazuya Muguruma", "Masaichi Ohira", "Hideki Wanibuchi", "Kosei Hirakawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0110736.t004", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Univariate_and_multivariate_analyses_with_respect_to_survival_/1232429", "title"=>"Univariate and multivariate analyses with respect to survival.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-07 02:52:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1783703"], "description"=>"<p>Correlation between proteins expression and clinicopathological variables.</p>", "links"=>[], "tags"=>["RBBP 6 siRNA", "proteomics technology", "13a", "Tandem mass spectrometry", "hspa", "Comparative Proteomics Analysis", "DCTPP", "RBBP 6", "csc", "GLG", "Gastric Cancer Stem Cells Cancer", "sp", "lymph node metastasis", "ck", "protein", "QSTAR Elite Liquid Chromatography", "OCUM", "krt", "aldoa", "vps"], "article_id"=>1232428, "categories"=>["Uncategorised"], "users"=>["Tamami Morisaki", "Masakazu Yashiro", "Anna Kakehashi", "Azusa Inagaki", "Haruhito Kinoshita", "Tatsunari Fukuoka", "Hiroaki Kasashima", "Go Masuda", "Katsunobu Sakurai", "Naoshi Kubo", "Kazuya Muguruma", "Masaichi Ohira", "Hideki Wanibuchi", "Kosei Hirakawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0110736.t003", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Correlation_between_proteins_expression_and_clinicopathological_variables_/1232428", "title"=>"Correlation between proteins expression and clinicopathological variables.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-07 02:52:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1783700"], "description"=>"<p>Clinicopathological characteristics of 300 gastric cancer patients.</p>", "links"=>[], "tags"=>["RBBP 6 siRNA", "proteomics technology", "13a", "Tandem mass spectrometry", "hspa", "Comparative Proteomics Analysis", "DCTPP", "RBBP 6", "csc", "GLG", "Gastric Cancer Stem Cells Cancer", "sp", "lymph node metastasis", "ck", "protein", "QSTAR Elite Liquid Chromatography", "OCUM", "krt", "aldoa", "vps"], "article_id"=>1232425, "categories"=>["Uncategorised"], "users"=>["Tamami Morisaki", "Masakazu Yashiro", "Anna Kakehashi", "Azusa Inagaki", "Haruhito Kinoshita", "Tatsunari Fukuoka", "Hiroaki Kasashima", "Go Masuda", "Katsunobu Sakurai", "Naoshi Kubo", "Kazuya Muguruma", "Masaichi Ohira", "Hideki Wanibuchi", "Kosei Hirakawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0110736.t001", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Clinicopathological_characteristics_of_300_gastric_cancer_patients_/1232425", "title"=>"Clinicopathological characteristics of 300 gastric cancer patients.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-07 02:52:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1783699"], "description"=>"<p>The overall survival of patients with gastric cancer in relation to the expression of RBBP6, GLG1, VPS13A, DCTPP1, HSPA9, HSPA4, ALDOA, and KRT18. The prognosis of patients with RBBP6, DCTPP1, HSPA4, and ALDOA expression was significantly poorer than those without expression in gastric cancer cases. The overall survival rate of RBBP6-positive cases was significantly less than that of RBBP6-negative cases in patients at stage III. In contrast, no significant correlations were observed between other proteins and patient survival.</p>", "links"=>[], "tags"=>["RBBP 6 siRNA", "proteomics technology", "13a", "Tandem mass spectrometry", "hspa", "Comparative Proteomics Analysis", "DCTPP", "RBBP 6", "csc", "GLG", "Gastric Cancer Stem Cells Cancer", "sp", "lymph node metastasis", "ck", "protein", "QSTAR Elite Liquid Chromatography", "OCUM", "krt", "aldoa", "vps"], "article_id"=>1232424, "categories"=>["Uncategorised"], "users"=>["Tamami Morisaki", "Masakazu Yashiro", "Anna Kakehashi", "Azusa Inagaki", "Haruhito Kinoshita", "Tatsunari Fukuoka", "Hiroaki Kasashima", "Go Masuda", "Katsunobu Sakurai", "Naoshi Kubo", "Kazuya Muguruma", "Masaichi Ohira", "Hideki Wanibuchi", "Kosei Hirakawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0110736.g004", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Overall_Survival_of_Patients_with_Gastric_Cancer_/1232424", "title"=>"Overall Survival of Patients with Gastric Cancer.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-07 02:52:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1783708", "https://ndownloader.figshare.com/files/1783709", "https://ndownloader.figshare.com/files/1783710", "https://ndownloader.figshare.com/files/1783711", "https://ndownloader.figshare.com/files/1783712", "https://ndownloader.figshare.com/files/1783713"], "description"=>"<div><p>Cancer stem cells (CSCs) are responsible for cancer progression, metastasis, and recurrence. To date, the specific markers of CSCs remain undiscovered. The aim of this study was to identify novel biomarkers of gastric CSCs for clinical diagnosis using proteomics technology. CSC-like SP cells, OCUM-12/SP cells, OCUM-2MD3/SP cells, and their parent OCUM-12 cells and OCUM-2MD3 cells were used in this study. Protein lysates from each cell line were analyzed using QSTAR Elite Liquid Chromatography with Tandem Mass Spectrometry, coupled with isobaric tags for relative and absolute quantitation technology. Candidate proteins detected by proteomics technology were validated by immunohistochemical analysis of 300 gastric cancers. Based on the results of LC-MS/MS, eight proteins, including RBBP6, GLG1, VPS13A, DCTPP1, HSPA9, HSPA4, ALDOA, and KRT18, were up-regulated in both OCUM-12/SP cells and OCUM-2MD3/SP cells when compared to their corresponding parent cells. RT-PCR analysis indicated that the expression level of <i>RBBP6, HSPA4, DCTPP1, HSPA9, VPS13A, ALDOA, GLG1</i>, and <i>CK18</i> was high in OCUM-12/SP and OCUM-2MD3/SP, in compared with the control of parent OCUM-12 and OCUM-2MD3. These proteins were significantly associated with advanced invasion depth, lymph node metastasis, distant metastasis, or advanced clinical stage. RBBP6, DCTPP1, HSPA4, and ALDOA expression in particular were significantly associated with a poor prognosis in the 300 gastric cancer patients. RBBP6 was determined to be an independent prognostic factor. The motility-stimulating ability of OCUM-12/SP cells and OCUM-2MD3/SP cells was inhibited by <i>RBBP6</i> siRNA. These findings might suggest that the eight proteins, RBBP6, GLG1, VPS13A, DCTPP1, HSPA9, HSPA4, ALDOA, and KRT18, utilizing comparative proteomics analysis, were perceived to be potential CSC markers of gastric cancer. Of the eight candidate proteins, RBBP6 was suggested to be a promising prognostic biomarker and a therapeutic target for gastric cancer.</p></div>", "links"=>[], "tags"=>["RBBP 6 siRNA", "proteomics technology", "13a", "Tandem mass spectrometry", "hspa", "Comparative Proteomics Analysis", "DCTPP", "RBBP 6", "csc", "GLG", "Gastric Cancer Stem Cells Cancer", "sp", "lymph node metastasis", "ck", "protein", "QSTAR Elite Liquid Chromatography", "OCUM", "krt", "aldoa", "vps"], "article_id"=>1232433, "categories"=>["Uncategorised"], "users"=>["Tamami Morisaki", "Masakazu Yashiro", "Anna Kakehashi", "Azusa Inagaki", "Haruhito Kinoshita", "Tatsunari Fukuoka", "Hiroaki Kasashima", "Go Masuda", "Katsunobu Sakurai", "Naoshi Kubo", "Kazuya Muguruma", "Masaichi Ohira", "Hideki Wanibuchi", "Kosei Hirakawa"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0110736.s001", "https://dx.doi.org/10.1371/journal.pone.0110736.s002", "https://dx.doi.org/10.1371/journal.pone.0110736.s003", "https://dx.doi.org/10.1371/journal.pone.0110736.s004", "https://dx.doi.org/10.1371/journal.pone.0110736.s005", "https://dx.doi.org/10.1371/journal.pone.0110736.s006"], "stats"=>{"downloads"=>7, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparative_Proteomics_Analysis_of_Gastric_Cancer_Stem_Cells_/1232433", "title"=>"Comparative Proteomics Analysis of Gastric Cancer Stem Cells", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-11-07 02:52:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1783701"], "description"=>"a<p>ratio, OCUM-12/SP ratio: OCUM-12/SP cell compared with parent OCUM-12 cell. OCUM-2MD3/SP ratio: OCUM-2MD3/SP cell compared with parent OCUM-2MD3 cell.</p>b<p>↑, proteins expressed only in SP cells but not parent cells.</p>c<p>Location: C, cytoplasm; PM, plasma membrane; ES, extracellular space; N, nucleus.</p>d<p>Type: En, enzyme; K, kinase; Tp, transporter; O, other; TR, transcriptional regulator.</p><p>NA, not available.</p><p>Proteins increased in both OCUM-12/SP and OCUM-2MD3/SP cells compared to their parent cells detected by QSTAR Elite LC/MS/MS.</p>", "links"=>[], "tags"=>["RBBP 6 siRNA", "proteomics technology", "13a", "Tandem mass spectrometry", "hspa", "Comparative Proteomics Analysis", "DCTPP", "RBBP 6", "csc", "GLG", "Gastric Cancer Stem Cells Cancer", "sp", "lymph node metastasis", "ck", "protein", "QSTAR Elite Liquid Chromatography", "OCUM", "krt", "aldoa", "vps"], "article_id"=>1232426, "categories"=>["Uncategorised"], "users"=>["Tamami Morisaki", "Masakazu Yashiro", "Anna Kakehashi", "Azusa Inagaki", "Haruhito Kinoshita", "Tatsunari Fukuoka", "Hiroaki Kasashima", "Go Masuda", "Katsunobu Sakurai", "Naoshi Kubo", "Kazuya Muguruma", "Masaichi Ohira", "Hideki Wanibuchi", "Kosei Hirakawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0110736.t002", "stats"=>{"downloads"=>7, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proteins_increased_in_both_OCUM_12_SP_and_OCUM_2MD3_SP_cells_compared_to_their_parent_cells_detected_by_QSTAR_Elite_LC_MS_MS_/1232426", "title"=>"Proteins increased in both OCUM-12/SP and OCUM-2MD3/SP cells compared to their parent cells detected by QSTAR Elite LC/MS/MS.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-07 02:52:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1783696"], "description"=>"<p>(a) Expression in cancer cells. RBBP6, DCTPP1, and HSPA9 expression were observed primarily in the cytoplasm and nucleus. GLG1, VPS13A, HSPA4, ALDOA, and KRT18 were expressed in the cytoplasm. (b), Expression in epithelial cells. RBBP6, GLG1, VPS13A, DCTPP1, and HSPA9 expression were found some cells in the epithelial gland. KRT18 were expressed in most epithelial cells. HSPA4 and ALDOA expression was not found in normal cells.</p>", "links"=>[], "tags"=>["RBBP 6 siRNA", "proteomics technology", "13a", "Tandem mass spectrometry", "hspa", "Comparative Proteomics Analysis", "DCTPP", "RBBP 6", "csc", "GLG", "Gastric Cancer Stem Cells Cancer", "sp", "lymph node metastasis", "ck", "protein", "QSTAR Elite Liquid Chromatography", "OCUM", "krt", "aldoa", "vps"], "article_id"=>1232421, "categories"=>["Uncategorised"], "users"=>["Tamami Morisaki", "Masakazu Yashiro", "Anna Kakehashi", "Azusa Inagaki", "Haruhito Kinoshita", "Tatsunari Fukuoka", "Hiroaki Kasashima", "Go Masuda", "Katsunobu Sakurai", "Naoshi Kubo", "Kazuya Muguruma", "Masaichi Ohira", "Hideki Wanibuchi", "Kosei Hirakawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0110736.g003", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunohistochemical_Determination_/1232421", "title"=>"Immunohistochemical Determination.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-07 02:52:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1783692"], "description"=>"<p>(A), OCUM-12/SP and OCUM-2MD3/SP showed higher level of <i>RBBP6</i> mRNA expression than their parent cells, OCUM-12 and OCUM-2MD3. RBBP6 expression in OCUM-12/SP and OCUM-2MD3/SP cells was effectively downregulated by si<i>RBBP6</i> transfection. (B), Representative images of invading OCUM-12/SP cells showed the number of cancer cells invading the pore membrane filter was decreased by <i>RBBP6</i> siRNA treatment. si<i>RBBP6</i> transfection for OCUM12/SP cells significantly inhibited the invasion abilities. Data are presented as the mean and SD (error bars) of four experiments. * <i>p</i><0.05, ** <i>p</i><0.01. (C), si<i>RBBP6</i> treatment for OCUM12/SP and OCUM-2MD3/SP cells significantly inhibited the migration abilities, in comparison with that of the control of <i>negative-siRNA</i> treatment. Data are presented as the mean and SD (error bars) of four experiments. * <i>p</i><0.05, ** <i>p</i><0.01.</p>", "links"=>[], "tags"=>["RBBP 6 siRNA", "proteomics technology", "13a", "Tandem mass spectrometry", "hspa", "Comparative Proteomics Analysis", "DCTPP", "RBBP 6", "csc", "GLG", "Gastric Cancer Stem Cells Cancer", "sp", "lymph node metastasis", "ck", "protein", "QSTAR Elite Liquid Chromatography", "OCUM", "krt", "aldoa", "vps"], "article_id"=>1232417, "categories"=>["Uncategorised"], "users"=>["Tamami Morisaki", "Masakazu Yashiro", "Anna Kakehashi", "Azusa Inagaki", "Haruhito Kinoshita", "Tatsunari Fukuoka", "Hiroaki Kasashima", "Go Masuda", "Katsunobu Sakurai", "Naoshi Kubo", "Kazuya Muguruma", "Masaichi Ohira", "Hideki Wanibuchi", "Kosei Hirakawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0110736.g002", "stats"=>{"downloads"=>0, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_siRBBP6_transfection_into_gastric_cancer_cells_/1232417", "title"=>"<i>siRBBP6</i> transfection into gastric cancer cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-07 02:52:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1783689"], "description"=>"<p>(A) Localization; (B) Biological processes of identified proteins. We categorized the proteins based on their functional assignments using Ingenuity Pathway Analysis. The molecular functions reported include cell death, metabolism, cellular organization, metabolism of DNA, protein degradation, processing of RNA, production of reactive oxygen species, nitric oxide, molecular transport, cell cycle, folding protein, and cellular movement. % = 100 X number of identified proteins/all 932 proteins analyzed. (C), A Venn diagram confer to <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0110736#pone-0110736-t002\" target=\"_blank\">Table 2</a>. Forty proteins were significantly increased in OCUM-12/SP cells compared to their parent OCUM-12 cells. Thirty-five proteins were significantly increased in OCUM-2MD3/SP cells compared to their parent OCUM-2MD3cells. Eight candidate proteins, RBBP6, HSPA4, HSPA9, GLG1, DCTPP1, VPS13A, CK18 and ALDOA, overlap in both OCUM-12/SP and OCUM-2MD3/SP cells. (D), mRNA expression. RT-PCR analysis indicated that the expression level of <i>RBBP6, HSPA4, DCTPP1, HSPA9, VPS13A, ALDOA, GLG1</i>, and <i>CK18</i> was high in OCUM-12/SP (9.15 fold, 9.36 fold, 4.14 fold, 7.80 fold, 2.08 fold, 1.46 fold, 3.44 fold, and 1.99 fold, respectively) and OCUM-2MD3/SP (6.15 fold, 1.71 fold, 2.33 fold, 2.30 fold, 2.03 fold, 1.32 fold, 1.35 fold, and 1.31 fold, respectively), in compared with the control of parent OCUM-12 and OCUM-2MD3. (E), Correlation of Signaling Pathways between RBBP6 and Differentially-Expressed Proteins in CSC-like SP cells. RBBP6 is over-expressed (red) in CSC-like SP cells (OCUM-12/SP cells and OCUM-2MD3/SP cells). Hsp90, HSPA4, and TAGLN2 (pink) up-regulated in CSC-like SP cells were associated with the RBBP6 signaling pathway.</p>", "links"=>[], "tags"=>["RBBP 6 siRNA", "proteomics technology", "13a", "Tandem mass spectrometry", "hspa", "Comparative Proteomics Analysis", "DCTPP", "RBBP 6", "csc", "GLG", "Gastric Cancer Stem Cells Cancer", "sp", "lymph node metastasis", "ck", "protein", "QSTAR Elite Liquid Chromatography", "OCUM", "krt", "aldoa", "vps"], "article_id"=>1232415, "categories"=>["Uncategorised"], "users"=>["Tamami Morisaki", "Masakazu Yashiro", "Anna Kakehashi", "Azusa Inagaki", "Haruhito Kinoshita", "Tatsunari Fukuoka", "Hiroaki Kasashima", "Go Masuda", "Katsunobu Sakurai", "Naoshi Kubo", "Kazuya Muguruma", "Masaichi Ohira", "Hideki Wanibuchi", "Kosei Hirakawa"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0110736.g001", "stats"=>{"downloads"=>2, "page_views"=>41, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ingenuity_Pathway_Analysis_IPA_of_Proteins_Expressed_in_SP_Cells_/1232415", "title"=>"Ingenuity Pathway Analysis (IPA) of Proteins Expressed in SP Cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-07 02:52:49"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"30", "full-text"=>"57", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"30", "full-text"=>"28", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"28", "full-text"=>"27", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"51", "full-text"=>"40", "pdf"=>"26", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"27", "full-text"=>"32", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"30", "full-text"=>"28", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"34", "full-text"=>"26", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"39", "full-text"=>"36", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"40", "full-text"=>"37", "pdf"=>"23", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"47", "full-text"=>"45", "pdf"=>"28", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"56", "full-text"=>"56", "pdf"=>"31", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"35", "full-text"=>"25", "pdf"=>"24", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"18", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"29", "full-text"=>"22", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"29", "full-text"=>"43", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"18", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"20", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[306, 482]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[292, 461]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[271]}, {"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[262]}]}
Loading … Spinner
There are currently no alerts