The Bile Acid-Sensitive Ion Channel (BASIC) Is Activated by Alterations of Its Membrane Environment
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"The Bile Acid-Sensitive Ion Channel (BASIC) Is activated by alterations of its membrane environment", "type"=>"journal", "authors"=>[{"first_name"=>"Axel", "last_name"=>"Schmidt", "scopus_author_id"=>"56412343800"}, {"first_name"=>"Pia", "last_name"=>"Lenzig", "scopus_author_id"=>"56413056000"}, {"first_name"=>"Adrienne", "last_name"=>"Oslender-Bujotzek", "scopus_author_id"=>"56413049300"}, {"first_name"=>"Jana", "last_name"=>"Kusch", "scopus_author_id"=>"7003361221"}, {"first_name"=>"Susana Dias", "last_name"=>"Lucas", "scopus_author_id"=>"20434060000"}, {"first_name"=>"Stefan", "last_name"=>"Gründer", "scopus_author_id"=>"7004529526"}, {"first_name"=>"Dominik", "last_name"=>"Wiemuth", "scopus_author_id"=>"16679963200"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84909993096", "pui"=>"600407306", "doi"=>"10.1371/journal.pone.0111549", "sgr"=>"84909993096", "pmid"=>"25360526"}, "id"=>"9e23888f-1dd8-33a3-8036-5acf5bf14ea7", "abstract"=>"The bile acid-sensitive ion channel (BASIC) is a member of the DEG/ENaC family of ion channels. Channels of this family are characterized by a common structure, their physiological functions and modes of activation, however, are diverse. Rat BASIC is expressed in brain, liver and intestinal tract and activated by bile acids. The physiological function of BASIC and its mechanism of bile acid activation remain a puzzle. Here we addressed the question whether amphiphilic bile acids activate BASIC by directly binding to the channel or indirectly by altering the properties of the surrounding membrane. We show that membrane-active substances other than bile acids also affect the activity of BASIC and that activation by bile acids and other membrane-active substances is non-additive, suggesting that BASIC is sensitive for changes in its membrane environment. Furthermore based on results from chimeras between BASIC and ASIC1a, we show that the extracellular and the transmembrane domains are important for membrane sensitivity.", "link"=>"http://www.mendeley.com/research/bile-acidsensitive-ion-channel-basic-activated-alterations-membrane-environment", "reader_count"=>11, "reader_count_by_academic_status"=>{"Librarian"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>2, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Librarian"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>2, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Agricultural and Biological Sciences"=>5, "Medicine and Dentistry"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemical Engineering"=>1, "Physics and Astronomy"=>1, "Chemistry"=>1, "Psychology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>5}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Chemical Engineering"=>{"Chemical Engineering"=>1}}, "reader_count_by_country"=>{"Portugal"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84909993096"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84909993096?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84909993096&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84909993096&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84909993096", "dc:identifier"=>"SCOPUS_ID:84909993096", "eid"=>"2-s2.0-84909993096", "dc:title"=>"The Bile Acid-Sensitive Ion Channel (BASIC) Is activated by alterations of its membrane environment", "dc:creator"=>"Schmidt A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"10", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-10-31", "prism:coverDisplayDate"=>"31 October 2014", "prism:doi"=>"10.1371/journal.pone.0111549", "citedby-count"=>"12", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Rheinisch-Westfälische Technische Hochschule Aachen", "affiliation-city"=>"Aachen", "affiliation-country"=>"Germany"}], "pubmed-id"=>"25360526", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e111549", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0111549", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"4", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"37", "xml_views"=>"4", "html_views"=>"189"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"2", "html_views"=>"68"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"33"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"32"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"3", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1774725"], "description"=>"<p>A, B) Left panel, schematic drawings of chimeras; rASIC1a portions are depicted as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111549#pone-0111549-g005\" target=\"_blank\">Fig. 5A</a>, rBASIC portions are depicted in white. Residues defining the borders of the ASIC1a and BASIC sequences are shown. Middle panel, representative traces of currents induced by the subsequent application of pH 6.5 and 2 mM UDCA followed by the removal of divalent cations (–Ca<sup>2+</sup>). Right panel, quantitative comparison of current amplitudes induced by pH 6.5, UDCA and divalents removal. Error bars represent SEM; <i>n</i> = 6–12.</p>", "links"=>[], "tags"=>["amphiphilic bile acids", "asic", "basic", "deg", "membrane", "bile acids", "bile acid activation"], "article_id"=>1225197, "categories"=>["Biological Sciences"], "users"=>["Axel Schmidt", "Pia Lenzig", "Adrienne Oslender-Bujotzek", "Jana Kusch", "Susana Dias Lucas", "Stefan Gründer", "Dominik Wiemuth"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111549.g009", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Several_regions_of_the_ECD_of_rBASIC_are_involved_in_bile_acid_sensitivity_/1225197", "title"=>"Several regions of the ECD of rBASIC are involved in bile acid sensitivity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-31 03:38:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1774710"], "description"=>"<p>A) Representative current traces showing the concentration-dependent activation of BASIC by TNP. Upper panel, increasing concentrations of TNP were applied to a rBASIC expressing oocyte. Lower panel, increasing concentrations of TNP were co-applied with 2 mM UDCA. Dotted lines represent the 0 current level. B) Concentration-response curves for TNP in the absence (open circles) and presence (closed circles) of 2 mM UDCA. Currents were normalized to the maximum currents in the presence of TNP, which had amplitudes of 3.9±0.4 µA and 12.1±2.5 µA (n = 10) in the absence and presence of 2 mM UDCA, respectively. The current amplitude of 2 mM UDCA alone was 4.1±0.4 µA (<i>n</i> = 10). Error bars represent SEM, curves were fitted to the Hill equation. C) Representative current trace of a non-injected oocyte treated with increasing concentrations of TNP. D) Representative current traces showing the concentration-dependent inhibition of BASIC by Gd<sup>3+</sup>. Upper panel, increasing concentrations of Gd<sup>3+</sup> were applied to a rBASIC expressing oocyte. Lower panel, increasing concentrations of Gd<sup>3+</sup> were co-applied with 2 mM UDCA. Dotted lines represent the 0 current level. E) Concentration-response curves for Gd<sup>3+</sup> in the absence (closed circles) and presence (open circles) of 2 mM UDCA. Currents were normalized to the maximum currents in the absence of Gd<sup>3+</sup>, which had amplitudes of 1.2±0.2 µA and 3.2±0.6 µA (<i>n</i> = 10) in the absence and presence of 2 mM UDCA, respectively. Error bars represent SEM, curves were fitted to the Hill equation. F) Representative current trace of a non-injected oocyte treated with increasing concentrations of Gd<sup>3+</sup>.</p>", "links"=>[], "tags"=>["amphiphilic bile acids", "asic", "basic", "deg", "membrane", "bile acids", "bile acid activation"], "article_id"=>1225182, "categories"=>["Biological Sciences"], "users"=>["Axel Schmidt", "Pia Lenzig", "Adrienne Oslender-Bujotzek", "Jana Kusch", "Susana Dias Lucas", "Stefan Gründer", "Dominik Wiemuth"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111549.g002", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TNP_increases_while_Gd_3_decreases_BASIC_activity_in_a_dose_dependent_manner_/1225182", "title"=>"TNP increases while Gd<sup>3+</sup> decreases BASIC activity in a dose-dependent manner.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-31 03:38:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1774724"], "description"=>"<p>A) Left, scheme of ASIC1 showing the domain organization of one subunit as suggested by Jasti <i>et al.</i> based on the crystal structure of chicken ASIC1 <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111549#pone.0111549-Jasti1\" target=\"_blank\">[3]</a>. The ECD consists of five domains: palm (yellow), thumb (green), finger (violet), knuckle (blue) and β-ball (orange). Right, linear scheme of ASIC1a showing the domains in the same color coding as in the scheme on the left. Presumably, BASIC shows a similar domain organization as ASIC1a. B) Left panel, schematic drawings of rASIC1a, rBASIC and chimeras; rASIC1a is depicted as in A), for clear distinction, rBASIC is depicted in white. Residues defining the borders of the rASIC1a and rBASIC sequences are shown. Middle panel, representative traces of currents induced by the subsequent application of pH 6.5 and 2 mM UDCA followed by the removal of divalent cations (–Ca<sup>2+</sup>). Right panel, quantitative comparison of current amplitudes induced by pH 6.5, UDCA and divalent cation removal. Error bars represent SEM; <i>n</i> = 6–12. Note that different amounts of cRNA were injected (rASIC1a, 0.8 ng; rBASIC, rASIC1a-rBASIC-loop and rBASIC-rASIC1a-loop, 8 ng, respectively).</p>", "links"=>[], "tags"=>["amphiphilic bile acids", "asic", "basic", "deg", "membrane", "bile acids", "bile acid activation"], "article_id"=>1225196, "categories"=>["Biological Sciences"], "users"=>["Axel Schmidt", "Pia Lenzig", "Adrienne Oslender-Bujotzek", "Jana Kusch", "Susana Dias Lucas", "Stefan Gründer", "Dominik Wiemuth"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111549.g008", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_ECD_of_rBASIC_is_involved_in_bile_acid_sensitivity_/1225196", "title"=>"The ECD of rBASIC is involved in bile acid sensitivity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-31 03:38:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1774720"], "description"=>"<p>A) Removal of cholesterol does not influence rBASIC activity. Quantitative comparison of DIMI-sensitive rBASIC currents from oocytes that were incubated in low Na<sup>+</sup> OR-2 medium containing 20 mM MβCD (grey bars) or 20 mM mannitol (control, black bars) for approximately 2 h prior to recordings. Current amplitudes in the absence of any stimulus (control), in the absence of extracellular Ca<sup>2+</sup> (−Ca<sup>2+</sup>) and in the presence of 2 mM UDCA are shown. B) Addition of cholesterol does not influence rBASIC activity. Quantitative comparison of DIMI-sensitive rBASIC current amplitudes before (black bar) and after 10 sec application of 2 mM MβCD-balanced water-soluble cholesterol (grey bar). Error bars represent SEM, <i>n</i> = 8.</p>", "links"=>[], "tags"=>["amphiphilic bile acids", "asic", "basic", "deg", "membrane", "bile acids", "bile acid activation"], "article_id"=>1225193, "categories"=>["Biological Sciences"], "users"=>["Axel Schmidt", "Pia Lenzig", "Adrienne Oslender-Bujotzek", "Jana Kusch", "Susana Dias Lucas", "Stefan Gründer", "Dominik Wiemuth"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111549.g006", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cholesterol_does_not_affect_rBASIC_activity_/1225193", "title"=>"Cholesterol does not affect rBASIC activity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-31 03:38:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1774719"], "description"=>"<p>A) Representative current traces of BASIC showing the concentration-dependent activation of BASIC by UDCA in the absence and presence of membrane active substances (CPZ, 500 µM; TNP, 2 mM; TX100, 100 µM; NL, 500 µM or SDS, 400 µM). Dotted lines represent the 0 current level B) Concentration-response curves for UDCA in the presence and absence of membrane active substances. Currents were normalized to the maximum current at 5 mM UDCA, which was 23.8±2.4 µA. Error bars = S.E.M., <i>n</i> = 10. Curves represent fits to the Hill equation (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111549#pone.0111549.e001\" target=\"_blank\">equation (1</a>)).</p>", "links"=>[], "tags"=>["amphiphilic bile acids", "asic", "basic", "deg", "membrane", "bile acids", "bile acid activation"], "article_id"=>1225191, "categories"=>["Biological Sciences"], "users"=>["Axel Schmidt", "Pia Lenzig", "Adrienne Oslender-Bujotzek", "Jana Kusch", "Susana Dias Lucas", "Stefan Gründer", "Dominik Wiemuth"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111549.g005", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Bile_acids_and_other_membrane_active_substances_activate_BASIC_by_a_similar_mechanism_/1225191", "title"=>"Bile acids and other membrane-active substances activate BASIC by a similar mechanism.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-31 03:38:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1774718"], "description"=>"<p>A–C) Representative current traces (black) of BASIC-expressing oocytes showing that the application of 400 µM SDS (A, left panel), 500 µM N-Lauroylsarcosine (NL) (B) and 100 µM Triton-X 100 (C) increased the activity of BASIC. The application of 100 µM diminazene (DIMI) alone or together with SDS (A, right panel), NL (B) or Triton-X 100 (C) inhibited BASIC currents. Non-injected oocytes did not respond to the application of the substances (grey traces). Dotted lines represent the 0 current level. (D) Quantitative comparison of current amplitudes induced by SDS, NL or Triton-X 100. Currents were normalized to the DIMI-sensitive current in the absence of stimulatory substances, which had an amplitude of 0.25±0.03 µA (<i>n</i> = 8). Error bars represent SEM. Statistical significances were tested using one-way ANOVA followed by Tukey's multiple comparison test versus control, **p<0.01, ***p<0.001, <i>n</i> = 8. (E) Representative current traces of oocytes expressing ASIC1a or HyNaC2/3/5. Application of pH 5 or 5 µM RFamide I, respectively, activated the channels, while application of 400 µM SDS, 500 µM NL or 100 µM Triton-X 100 did not.</p>", "links"=>[], "tags"=>["amphiphilic bile acids", "asic", "basic", "deg", "membrane", "bile acids", "bile acid activation"], "article_id"=>1225190, "categories"=>["Biological Sciences"], "users"=>["Axel Schmidt", "Pia Lenzig", "Adrienne Oslender-Bujotzek", "Jana Kusch", "Susana Dias Lucas", "Stefan Gründer", "Dominik Wiemuth"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111549.g004", "stats"=>{"downloads"=>2, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Other_membrane_active_substances_which_are_structurally_unrelated_to_bile_acids_also_activate_BASIC_/1225190", "title"=>"Other membrane-active substances, which are structurally unrelated to bile acids also activate BASIC.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-31 03:38:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1774727"], "description"=>"<p>Left panel, schematic drawings of chimeras; rASIC1a portions are depicted as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111549#pone-0111549-g005\" target=\"_blank\">Fig. 5A</a>, rBASIC portions are depicted in white. Residues defining the borders of the ASIC1a and BASIC sequences are shown. Middle panel, representative traces of currents induced by the subsequent application of pH 6.5, 2 mM UDCA, and removal of divalent cations (–Ca<sup>2+</sup>). Right panel, quantitative comparison of current amplitudes induced by pH 6.5, UDCA and divalent cation removal. Error bars represent SEM; <i>n</i> = 6–12.</p>", "links"=>[], "tags"=>["amphiphilic bile acids", "asic", "basic", "deg", "membrane", "bile acids", "bile acid activation"], "article_id"=>1225199, "categories"=>["Biological Sciences"], "users"=>["Axel Schmidt", "Pia Lenzig", "Adrienne Oslender-Bujotzek", "Jana Kusch", "Susana Dias Lucas", "Stefan Gründer", "Dominik Wiemuth"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111549.g010", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_TMDs_of_rBASIC_are_involved_in_bile_acid_sensitivity_/1225199", "title"=>"The TMDs of rBASIC are involved in bile acid sensitivity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-31 03:38:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1774708"], "description"=>"<p>A) Representative current traces of an oocyte expressing BASIC (black trace) and a water-injected control oocyte (grey trace). Application of 2 mM UDCA induced a typical BASIC current. Co-application of 0.5 mM CPZ strongly decreased the UDCA-dependent current while co-application of 2 mM TNP strongly increased the current amplitude. The UDCA-dependent current was completely abolished by 100 µM Gd<sup>3+</sup>. Dotted line represents the 0 current level. B) Quantitative comparison of current amplitudes induced by UDCA alone or together with 0.5 mM CPZ, 2 mM TNP or 100 µM Gd<sup>3+</sup>. Currents were normalized to the current induced by UDCA alone, which had an amplitude of 1.1±0.2 µA (<i>n</i> = 10). Error bars represent SEM. Statistical significances were tested using one-way ANOVA followed by Tukey's multiple comparison test versus UDCA, **p<0.01, ***p<0.001.</p>", "links"=>[], "tags"=>["amphiphilic bile acids", "asic", "basic", "deg", "membrane", "bile acids", "bile acid activation"], "article_id"=>1225180, "categories"=>["Biological Sciences"], "users"=>["Axel Schmidt", "Pia Lenzig", "Adrienne Oslender-Bujotzek", "Jana Kusch", "Susana Dias Lucas", "Stefan Gründer", "Dominik Wiemuth"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111549.g001", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_BASIC_responses_to_bile_acid_are_modulated_by_the_membrane_active_substances_chlorpromazine_trinitrophenol_and_Gd_3_/1225180", "title"=>"BASIC responses to bile acid are modulated by the membrane active substances chlorpromazine, trinitrophenol and Gd<sup>3+</sup>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-31 03:38:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1774722"], "description"=>"<p>A) Representative current traces of mBASIC-expressing oocytes showing that the application of 2 mM UDCA, 500 µM CPZ, 10 mM TNP, 100 µM Triton-X 100, 500 µM NL or 400 µM SDS change the activity of mBASIC. Dotted lines represent the 0 current level. To evaluate the amiloride-sensitive current 100 µM amiloride (AMI) was applied after wash-out of the membrane active substances. (B) Quantitative comparison of current amplitudes induced by UDCA, CPZ, TNP, Triton-X 100, NL or SDS. Currents were normalized to the amiloride-sensitive current, which had an amplitude of 12.3±2.4 µA (<i>n</i> = 56). Error bars represent SEM. Statistical significances were tested using one-way ANOVA followed by Tukey's multiple comparison test versus control, *p<0.05, **p<0.01, ***p<0.001, <i>n</i> = 8.</p>", "links"=>[], "tags"=>["amphiphilic bile acids", "asic", "basic", "deg", "membrane", "bile acids", "bile acid activation"], "article_id"=>1225194, "categories"=>["Biological Sciences"], "users"=>["Axel Schmidt", "Pia Lenzig", "Adrienne Oslender-Bujotzek", "Jana Kusch", "Susana Dias Lucas", "Stefan Gründer", "Dominik Wiemuth"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111549.g007", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mouse_BASIC_is_also_affected_by_membrane_active_substances_/1225194", "title"=>"Mouse BASIC is also affected by membrane active substances.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-31 03:38:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1774716"], "description"=>"<p>A) Confocal images of patch pipettes containing outside-out patches excised from <i>Xenopus laevis</i> oocytes. Left panel, membrane patch excised from an oocyte expressing rBASIC, before (upper panel) and after (lower panel) application of UDCA-NBD/UDCA (50 µM/1.6 mM). Right panel, membrane patch excised from an uninjected control oocyte, before (upper panel) and after (lower panel) application of UDCA-NBD/UDCA. Green fluorescence signals originated from UDCA-NBD binding to the membrane, red fluorescence signal originated from DY647 background dye (1 µM) staining the bath solution. The transmission channel is overlayed. Scale bar = 5 µm. B) Binding time courses and current time courses after sudden UDCA-NBD/UDCA concentration jumps, obtained from a membrane patch excised from an rBASIC expressing oocyte (left panel) and a membrane patch excised from an uninjected control oocyte (right panel). Green traces represent fluorescence signals induced by UDCA-NBD binding and unbinding to the patch membranes (for clarity, increase in fluorescence is depicted as downward deflection). Grey traces represent simultaneously recorded current traces. Averages of three consecutive measurements are shown. Time courses of activation/binding and deactivation/unbinding were fitted by the sum of two exponentials (equations (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111549#pone.0111549.e002\" target=\"_blank\">2a</a>) and (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111549#pone.0111549.e003\" target=\"_blank\">2b</a>)). τ<sub>fast</sub>, τ<sub>slow</sub> represent fast and slow time constants for rBASIC activation/inactivation and UDCA-NBD binding/unbinding events (a, rBASIC activation; b, UDCA-NBD binding; d, rBASIC deactivation; u, UDCA-NBD unbinding). C) and D) Quantitative comparison of slow and fast time constants of rBASIC activation and UDCA-NBD binding (C) and rBASIC deactivation and UDCA-NBD unbinding (D). Error bars represent SEM; <i>n</i> = 3–7.</p>", "links"=>[], "tags"=>["amphiphilic bile acids", "asic", "basic", "deg", "membrane", "bile acids", "bile acid activation"], "article_id"=>1225188, "categories"=>["Biological Sciences"], "users"=>["Axel Schmidt", "Pia Lenzig", "Adrienne Oslender-Bujotzek", "Jana Kusch", "Susana Dias Lucas", "Stefan Gründer", "Dominik Wiemuth"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111549.g003", "stats"=>{"downloads"=>4, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Correlation_of_UDCA_membrane_binding_and_rBASIC_activation_/1225188", "title"=>"Correlation of UDCA membrane binding and rBASIC activation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-10-31 03:38:19"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"24", "full-text"=>"19", "pdf"=>"15", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"21", "full-text"=>"18", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"9", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"3", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"28", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[267]}, {"subject_area"=>"/Biology and life sciences/Biophysics", "average_usage"=>[297, 441]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[310]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[280]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences/Physiology", "average_usage"=>[278]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[271]}, {"subject_area"=>"/Physical sciences/Physics", "average_usage"=>[266]}]}
Loading … Spinner
There are currently no alerts