Bioartificial Heart: A Human-Sized Porcine Model – The Way Ahead
Publication Date
November 03, 2014
Journal
PLOS ONE
Authors
Alexander Weymann, Nikhil Prakash Patil, Anton Sabashnikov, Philipp Jungebluth, et al
Volume
9
Issue
11
Pages
e111591
DOI
https://dx.plos.org/10.1371/journal.pone.0111591
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0111591
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/25365554
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4218780
Europe PMC
http://europepmc.org/abstract/MED/25365554
Web of Science
000345558100084
Scopus
84909953317
Mendeley
http://www.mendeley.com/research/bioartificial-heart-humansized-porcine-model-way-ahead
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Mendeley | Further Information

{"title"=>"Bioartificial heart: A human-sized porcine model - The way ahead", "type"=>"journal", "authors"=>[{"first_name"=>"Alexander", "last_name"=>"Weymann", "scopus_author_id"=>"36899452500"}, {"first_name"=>"Nikhil Prakash", "last_name"=>"Patil", "scopus_author_id"=>"37033170800"}, {"first_name"=>"Anton", "last_name"=>"Sabashnikov", "scopus_author_id"=>"53464306800"}, {"first_name"=>"Philipp", "last_name"=>"Jungebluth", "scopus_author_id"=>"15822561200"}, {"first_name"=>"Sevil", "last_name"=>"Korkmaz", "scopus_author_id"=>"25947512000"}, {"first_name"=>"Shiliang", "last_name"=>"Li", "scopus_author_id"=>"55484853000"}, {"first_name"=>"Gabor", "last_name"=>"Veres", "scopus_author_id"=>"57189698742"}, {"first_name"=>"Pal", "last_name"=>"Soos", "scopus_author_id"=>"7003676611"}, {"first_name"=>"Roland", "last_name"=>"Ishtok", "scopus_author_id"=>"56412012700"}, {"first_name"=>"Nicole", "last_name"=>"Chaimow", "scopus_author_id"=>"55540426300"}, {"first_name"=>"Ines", "last_name"=>"Pätzold", "scopus_author_id"=>"7801462877"}, {"first_name"=>"Natalie", "last_name"=>"Czerny", "scopus_author_id"=>"56411958400"}, {"first_name"=>"Carsten", "last_name"=>"Schies", "scopus_author_id"=>"37071274700"}, {"first_name"=>"Bastian", "last_name"=>"Schmack", "scopus_author_id"=>"48661389200"}, {"first_name"=>"Aron Frederik", "last_name"=>"Popov", "scopus_author_id"=>"55552579500"}, {"first_name"=>"André Rüdiger", "last_name"=>"Simon", "scopus_author_id"=>"56496125800"}, {"first_name"=>"Matthias", "last_name"=>"Karck", "scopus_author_id"=>"7006019086"}, {"first_name"=>"Gabor", "last_name"=>"Szabo", "scopus_author_id"=>"35328512500"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84909953317", "sgr"=>"84909953317", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\r1932-6203 (Linking)", "pmid"=>"25365554", "doi"=>"10.1371/journal.pone.0111591", "pui"=>"600373145"}, "id"=>"fceb9b80-a829-3979-be63-f6c97d5c34ad", "abstract"=>"BACKGROUND: A bioartificial heart is a theoretical alternative to transplantation or mechanical left ventricular support. Native hearts decellularized with preserved architecture and vasculature may provide an acellular tissue platform for organ regeneration. We sought to develop a tissue-engineered whole-heart neoscaffold in human-sized porcine hearts.\\n\\nMETHODS: We decellularized porcine hearts (n = 10) by coronary perfusion with ionic detergents in a modified Langendorff circuit. We confirmed decellularization by histology, transmission electron microscopy and fluorescence microscopy, quantified residual DNA by spectrophotometry, and evaluated biomechanical stability with ex-vivo left-ventricular pressure/volume studies, all compared to controls. We then mounted the decellularized porcine hearts in a bioreactor and reseeded them with murine neonatal cardiac cells and human umbilical cord derived endothelial cells (HUVEC) under simulated physiological conditions.\\n\\nRESULTS: Decellularized hearts lacked intracellular components but retained specific collagen fibers, proteoglycan, elastin and mechanical integrity; quantitative DNA analysis demonstrated a significant reduction of DNA compared to controls (82.6±3.2 ng DNA/mg tissue vs. 473.2±13.4 ng DNA/mg tissue, p<0.05). Recellularized porcine whole-heart neoscaffolds demonstrated re-endothelialization of coronary vasculature and measurable intrinsic myocardial electrical activity at 10 days, with perfused organ culture maintained for up to 3 weeks.\\n\\nCONCLUSIONS: Human-sized decellularized porcine hearts provide a promising tissue-engineering platform that may lead to future clinical strategies in the treatment of heart failure.", "link"=>"http://www.mendeley.com/research/bioartificial-heart-humansized-porcine-model-way-ahead", "reader_count"=>47, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>6, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>16, "Other"=>1, "Student > Bachelor"=>5, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>6, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>16, "Other"=>1, "Student > Bachelor"=>5, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Engineering"=>11, "Biochemistry, Genetics and Molecular Biology"=>5, "Materials Science"=>1, "Medicine and Dentistry"=>13, "Agricultural and Biological Sciences"=>13}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>11}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>13}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>13}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}, "Unspecified"=>{"Unspecified"=>4}}, "reader_count_by_country"=>{"Poland"=>1}, "group_count"=>2}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1776554"], "description"=>"<p>Demonstration of multi-electrode array electric voltage undulations of up to 200 mV in a time scale from ca. 500–1000 ms (red arrows) as a measure of myocardial electrical activity.</p>", "links"=>[], "tags"=>["umbilical cord", "organ regeneration", "perfused organ culture", "BackgroundA bioartificial heart", "decellularized porcine hearts", "biomechanical stability", "Heart failure", "10 days", "acellular tissue platform", "Bioartificial Heart", "Native hearts decellularized", "intracellular components", "transmission electron microscopy", "fluorescence microscopy", "huvec", "DNA analysis", "collagen fibers", "conditions.ResultsDecellularized hearts", "Langendorff circuit"], "article_id"=>1226861, "categories"=>["Biological Sciences"], "users"=>["Alexander Weymann", "Nikhil Prakash Patil", "Anton Sabashnikov", "Philipp Jungebluth", "Sevil Korkmaz", "Shiliang Li", "Gábor Veres", "Pál Soós", "Roland Ishtok", "Nicole Chaimow", "Ines Pätzold", "Natalie Czerny", "Carsten Schies", "Bastian Schmack", "Aron-Frederik Popov", "André Rüdiger Simon", "Matthias Karck", "Gábor Szabó"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111591.g007", "stats"=>{"downloads"=>6, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Demonstration_of_multi_electrode_array_electric_voltage_undulations_of_up_to_200_mV_in_a_time_scale_from_ca_500_8211_1000_ms_red_arrows_as_a_measure_of_myocardial_electrical_activity_/1226861", "title"=>"Demonstration of multi-electrode array electric voltage undulations of up to 200 mV in a time scale from ca. 500–1000 ms (red arrows) as a measure of myocardial electrical activity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-03 02:49:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1776553"], "description"=>"<p>Cardiomyocytes <b>(A, 60× magnification)</b> and human umbilical vein endothelial cells (HUVEC) <b>(B, 60× magnification)</b> were reseeded in the decellularized porcine heart (center). Histological analysis demonstrated neonatal cardiomyocytes around the injection sites in the left ventricular wall <b>(C, red arrow)</b>. Live/death assay demonstrated viability of the reseeded neonatal cardiomyocytes in the cultured bioartificial hearts (<b>D</b>). A de novo layer of endothelial cells was generated, indicated by positive PECAM-1 staining (<b>E, cross section left coronary artery</b>). However, the new surface cell layer of endothelial cells was partially interrupted <b>(E, red arrows)</b>. Scale bars, 200 µm.</p>", "links"=>[], "tags"=>["umbilical cord", "organ regeneration", "perfused organ culture", "BackgroundA bioartificial heart", "decellularized porcine hearts", "biomechanical stability", "Heart failure", "10 days", "acellular tissue platform", "Bioartificial Heart", "Native hearts decellularized", "intracellular components", "transmission electron microscopy", "fluorescence microscopy", "huvec", "DNA analysis", "collagen fibers", "conditions.ResultsDecellularized hearts", "Langendorff circuit"], "article_id"=>1226860, "categories"=>["Biological Sciences"], "users"=>["Alexander Weymann", "Nikhil Prakash Patil", "Anton Sabashnikov", "Philipp Jungebluth", "Sevil Korkmaz", "Shiliang Li", "Gábor Veres", "Pál Soós", "Roland Ishtok", "Nicole Chaimow", "Ines Pätzold", "Natalie Czerny", "Carsten Schies", "Bastian Schmack", "Aron-Frederik Popov", "André Rüdiger Simon", "Matthias Karck", "Gábor Szabó"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111591.g006", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Whole_organ_concept_/1226860", "title"=>"Whole organ concept.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-03 02:49:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1776550"], "description"=>"<p>Scale bars, 200 µm.</p>", "links"=>[], "tags"=>["umbilical cord", "organ regeneration", "perfused organ culture", "BackgroundA bioartificial heart", "decellularized porcine hearts", "biomechanical stability", "Heart failure", "10 days", "acellular tissue platform", "Bioartificial Heart", "Native hearts decellularized", "intracellular components", "transmission electron microscopy", "fluorescence microscopy", "huvec", "DNA analysis", "collagen fibers", "conditions.ResultsDecellularized hearts", "Langendorff circuit"], "article_id"=>1226857, "categories"=>["Biological Sciences"], "users"=>["Alexander Weymann", "Nikhil Prakash Patil", "Anton Sabashnikov", "Philipp Jungebluth", "Sevil Korkmaz", "Shiliang Li", "Gábor Veres", "Pál Soós", "Roland Ishtok", "Nicole Chaimow", "Ines Pätzold", "Natalie Czerny", "Carsten Schies", "Bastian Schmack", "Aron-Frederik Popov", "André Rüdiger Simon", "Matthias Karck", "Gábor Szabó"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111591.g003", "stats"=>{"downloads"=>12, "page_views"=>158, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Images_of_native_A_C_E_G_and_decellularized_ventricular_tissue_B_D_F_H_stained_with_DAPI_Masson_s_Trichrome_Stain_Masson_Herovici_s_Stain_Herovici_and_Movat_s_Pentachrome_Stain_Movat_revealed_absence_of_nuclear_staining_after_decellularization_B_and_stab/1226857", "title"=>"Images of native (A, C, E, G) and decellularized ventricular tissue (B, D, F, H) stained with DAPI, Masson's Trichrome Stain (Masson), Herovici's Stain (Herovici) and Movat's Pentachrome Stain (Movat) revealed absence of nuclear staining after decellularization (B) and stable preservation of extracellular matrix collagen, elastic fibers including large coronary vessels (D, H, red arrow) after the decellularization procedure.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-03 02:49:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1776548"], "description"=>"<p>All structures including the coronary vasculature (B, red arrow) are preserved. Hematoxylin and eosin (HE) staining of ventricular tissue before (C) and after perfusion decellularization (D) showing no remnant nuclear structures after treatment with SDS, with maintained extracellular matrix and coronary vessels (D, black arrow). Scale bars, 200 µm.</p>", "links"=>[], "tags"=>["umbilical cord", "organ regeneration", "perfused organ culture", "BackgroundA bioartificial heart", "decellularized porcine hearts", "biomechanical stability", "Heart failure", "10 days", "acellular tissue platform", "Bioartificial Heart", "Native hearts decellularized", "intracellular components", "transmission electron microscopy", "fluorescence microscopy", "huvec", "DNA analysis", "collagen fibers", "conditions.ResultsDecellularized hearts", "Langendorff circuit"], "article_id"=>1226855, "categories"=>["Biological Sciences"], "users"=>["Alexander Weymann", "Nikhil Prakash Patil", "Anton Sabashnikov", "Philipp Jungebluth", "Sevil Korkmaz", "Shiliang Li", "Gábor Veres", "Pál Soós", "Roland Ishtok", "Nicole Chaimow", "Ines Pätzold", "Natalie Czerny", "Carsten Schies", "Bastian Schmack", "Aron-Frederik Popov", "André Rüdiger Simon", "Matthias Karck", "Gábor Szabó"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111591.g002", "stats"=>{"downloads"=>0, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_images_of_a_porcine_heart_before_A_and_after_B_decellularization_with_sodium_dodecyl_sulfate_SDS_/1226855", "title"=>"Representative images of a porcine heart before (A) and after (B) decellularization with sodium dodecyl sulfate (SDS).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-03 02:49:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1776556"], "description"=>"<p>All values are expressed as mean ± SEM and adjusted to 1 mg lyophilized tissue sample.</p><p>Results of Extracellular Matrix Analysis.</p>", "links"=>[], "tags"=>["umbilical cord", "organ regeneration", "perfused organ culture", "BackgroundA bioartificial heart", "decellularized porcine hearts", "biomechanical stability", "Heart failure", "10 days", "acellular tissue platform", "Bioartificial Heart", "Native hearts decellularized", "intracellular components", "transmission electron microscopy", "fluorescence microscopy", "huvec", "DNA analysis", "collagen fibers", "conditions.ResultsDecellularized hearts", "Langendorff circuit"], "article_id"=>1226863, "categories"=>["Biological Sciences"], "users"=>["Alexander Weymann", "Nikhil Prakash Patil", "Anton Sabashnikov", "Philipp Jungebluth", "Sevil Korkmaz", "Shiliang Li", "Gábor Veres", "Pál Soós", "Roland Ishtok", "Nicole Chaimow", "Ines Pätzold", "Natalie Czerny", "Carsten Schies", "Bastian Schmack", "Aron-Frederik Popov", "André Rüdiger Simon", "Matthias Karck", "Gábor Szabó"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111591.t001", "stats"=>{"downloads"=>3, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Results_of_Extracellular_Matrix_Analysis_/1226863", "title"=>"Results of Extracellular Matrix Analysis.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-03 02:49:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1776552"], "description"=>"<p>Left ventricular peak pressure vs. volume. Decellularized hearts showed similar mechanical stability as native hearts with no significant differences in biomechanical behavior. All values are expressed as mean ± SEM.</p>", "links"=>[], "tags"=>["umbilical cord", "organ regeneration", "perfused organ culture", "BackgroundA bioartificial heart", "decellularized porcine hearts", "biomechanical stability", "Heart failure", "10 days", "acellular tissue platform", "Bioartificial Heart", "Native hearts decellularized", "intracellular components", "transmission electron microscopy", "fluorescence microscopy", "huvec", "DNA analysis", "collagen fibers", "conditions.ResultsDecellularized hearts", "Langendorff circuit"], "article_id"=>1226859, "categories"=>["Biological Sciences"], "users"=>["Alexander Weymann", "Nikhil Prakash Patil", "Anton Sabashnikov", "Philipp Jungebluth", "Sevil Korkmaz", "Shiliang Li", "Gábor Veres", "Pál Soós", "Roland Ishtok", "Nicole Chaimow", "Ines Pätzold", "Natalie Czerny", "Carsten Schies", "Bastian Schmack", "Aron-Frederik Popov", "André Rüdiger Simon", "Matthias Karck", "Gábor Szabó"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111591.g005", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Results_of_biomechanical_measurements_/1226859", "title"=>"Results of biomechanical measurements.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-03 02:49:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1776551"], "description"=>"<p>The extracellular matrix composition of the aortic wall (<b>C</b>) and aortic valve leaflet (<b>D</b>) was preserved after decellularization and showed no remnant nuclear material as demonstrated by hematoxylin and eosin (HE) staining (C, D) and TEM analysis (<b>box in D</b>). Also the aortic valve remained competent after decellularization (<b>E, F</b>).</p>", "links"=>[], "tags"=>["umbilical cord", "organ regeneration", "perfused organ culture", "BackgroundA bioartificial heart", "decellularized porcine hearts", "biomechanical stability", "Heart failure", "10 days", "acellular tissue platform", "Bioartificial Heart", "Native hearts decellularized", "intracellular components", "transmission electron microscopy", "fluorescence microscopy", "huvec", "DNA analysis", "collagen fibers", "conditions.ResultsDecellularized hearts", "Langendorff circuit"], "article_id"=>1226858, "categories"=>["Biological Sciences"], "users"=>["Alexander Weymann", "Nikhil Prakash Patil", "Anton Sabashnikov", "Philipp Jungebluth", "Sevil Korkmaz", "Shiliang Li", "Gábor Veres", "Pál Soós", "Roland Ishtok", "Nicole Chaimow", "Ines Pätzold", "Natalie Czerny", "Carsten Schies", "Bastian Schmack", "Aron-Frederik Popov", "André Rüdiger Simon", "Matthias Karck", "Gábor Szabó"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111591.g004", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Photomicrographs_of_unstained_tissue_samples_demonstrating_intact_coronary_vasculature_A_B_with_intact_third_and_fourth_level_vessels_A_red_arrows_/1226858", "title"=>"Photomicrographs of unstained tissue samples demonstrating intact coronary vasculature (A, B) with intact third- and fourth-level vessels (A, red arrows).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-03 02:49:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1776542"], "description"=>"<p>Whole-heart bioreactor: BIOSTAT B-DCU II and BioPAT DCU control tower (Sartorius Stedim Biotech GmbH, Germany).</p>", "links"=>[], "tags"=>["umbilical cord", "organ regeneration", "perfused organ culture", "BackgroundA bioartificial heart", "decellularized porcine hearts", "biomechanical stability", "Heart failure", "10 days", "acellular tissue platform", "Bioartificial Heart", "Native hearts decellularized", "intracellular components", "transmission electron microscopy", "fluorescence microscopy", "huvec", "DNA analysis", "collagen fibers", "conditions.ResultsDecellularized hearts", "Langendorff circuit"], "article_id"=>1226849, "categories"=>["Biological Sciences"], "users"=>["Alexander Weymann", "Nikhil Prakash Patil", "Anton Sabashnikov", "Philipp Jungebluth", "Sevil Korkmaz", "Shiliang Li", "Gábor Veres", "Pál Soós", "Roland Ishtok", "Nicole Chaimow", "Ines Pätzold", "Natalie Czerny", "Carsten Schies", "Bastian Schmack", "Aron-Frederik Popov", "André Rüdiger Simon", "Matthias Karck", "Gábor Szabó"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0111591.g001", "stats"=>{"downloads"=>4, "page_views"=>113, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Whole_heart_bioreactor_BIOSTAT_B_DCU_II_and_BioPAT_DCU_control_tower_Sartorius_Stedim_Biotech_GmbH_Germany_/1226849", "title"=>"Whole-heart bioreactor: BIOSTAT B-DCU II and BioPAT DCU control tower (Sartorius Stedim Biotech GmbH, Germany).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-03 02:49:32"}

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Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[267]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[266]}, {"subject_area"=>"/Medicine and health sciences/Surgical and invasive medical procedures", "average_usage"=>[248, 388]}]}
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