SPIDIA-RNA: Second External Quality Assessment for the Pre-Analytical Phase of Blood Samples Used for RNA Based Analyses
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{"title"=>"SPIDIA-RNA: Second External Quality Assessment for the Pre-Analytical Phase of Blood Samples Used for RNA Based Analyses", "type"=>"journal", "authors"=>[{"first_name"=>"Francesca", "last_name"=>"Malentacchi"}, {"first_name"=>"Mario", "last_name"=>"Pazzagli"}, {"first_name"=>"Lisa", "last_name"=>"Simi"}, {"first_name"=>"Claudio", "last_name"=>"Orlando"}, {"first_name"=>"Ralf", "last_name"=>"Wyrich"}, {"first_name"=>"Kalle", "last_name"=>"Gü Nther"}, {"first_name"=>"Paolo", "last_name"=>"Verderio"}, {"first_name"=>"Sara", "last_name"=>"Pizzamiglio"}, {"first_name"=>"Chiara Maura", "last_name"=>"Ciniselli"}, {"first_name"=>"Hui", "last_name"=>"Zhang"}, {"first_name"=>"Vlasta", "last_name"=>"Korenková"}, {"first_name"=>"Lynne", "last_name"=>"Rainen"}, {"first_name"=>"Tzachi", "last_name"=>"Bar"}, {"first_name"=>"Mikael", "last_name"=>"Kubista"}, {"first_name"=>"Stefania", "last_name"=>"Gelmini"}, {"first_name"=>"Junwen", "last_name"=>"Wang"}, {"first_name"=>"R", "last_name"=>"Wyrich"}, {"first_name"=>"K", "last_name"=>"Gü"}], "year"=>2014, "identifiers"=>{"doi"=>"10.1371/journal.pone.0112293", "pmid"=>"25384019", "issn"=>"1932-6203"}, "id"=>"1be88084-f5e5-3dab-ad6d-4afcd6ef0f94", "abstract"=>"One purpose of the EC funded project, SPIDIA, is to develop evidence-based quality guidelines for the pre-analytical handling of blood samples for RNA molecular testing. To this end, two pan-European External Quality Assessments (EQAs) were implemented. Here we report the results of the second SPIDIA-RNA EQA. This second study included modifications in the protocol related to the blood collection process, the shipping conditions and pre-analytical specimen handling for participants. Participating laboratories received two identical proficiency blood specimens collected in tubes with or without an RNA stabilizer. For pre-defined specimen storage times and temperatures, laboratories were asked to perform RNA extraction from whole blood according to their usual procedure and to return extracted RNA to the SPIDIA facility for further analysis. These RNA samples were evaluated for purity, yield, integrity, stability, presence of interfering substances, and gene expression levels for the validated markers of RNA stability: FOS, IL1B, IL8, GAPDH, FOSB and TNFRSF10c. Analysis of the gene expression results of FOS, IL8, FOSB, and TNFRSF10c, however, indicated that the levels of these transcripts were significantly affected by blood collection tube type and storage temperature. These results demonstrated that only blood collection tubes containing a cellular RNA stabilizer allowed reliable gene expression analysis within 48 h from blood collection for all the genes investigated. The results of these two EQAs have been proposed for use in the development of a Technical Specification by the European Committee for Standardization. Citation: Malentacchi F, Pazzagli M, Simi L, Orlando C, Wyrich R, et al. (2014) SPIDIA-RNA: Second External Quality Assessment for the Pre-Analytical Phase of Blood Samples Used for RNA Based Analyses. PLoS ONE 9(11): e112293.", "link"=>"http://www.mendeley.com/research/spidiarna-second-external-quality-assessment-preanalytical-phase-blood-samples-used-rna-based-analys", "reader_count"=>2, "reader_count_by_academic_status"=>{"Unspecified"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>2}, "reader_count_by_subdiscipline"=>{"Unspecified"=>{"Unspecified"=>2}}, "group_count"=>1}

Scopus | Further Information

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Figshare

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  • {"files"=>["https://ndownloader.figshare.com/files/1786598"], "description"=>"<p>Overall distribution of FOS (A) and IL1B (B) according to time storage. Each dot represents the expression levels of each individual (black) or pool (gray) samples for each time; the dashed lines indicate the time-trend for each sample. The continuous lines indicate the overall trend for individual (black) and pool (gray) samples. The horizontal dot-dashed line indicates the expected value of T<sub>0</sub>.</p>", "links"=>[], "tags"=>["TNFRSF 10c Analysis", "gene expression results", "SPIDIA", "eqa", "blood collection tubes", "proficiency blood specimens", "gapdh", "fos", "ec", "gene expression analysis", "fosb", "blood collection process", "1b", "gene expression levels", "blood collection tube type", "External Quality Assessments", "il", "RNA stabilizer"], "article_id"=>1234953, "categories"=>["Uncategorised"], "users"=>["Francesca Malentacchi", "Mario Pazzagli", "Lisa Simi", "Claudio Orlando", "Ralf Wyrich", "Kalle Günther", "Paolo Verderio", "Sara Pizzamiglio", "Chiara Maura Ciniselli", "Hui Zhang", "Vlasta Korenková", "Lynne Rainen", "Tzachi Bar", "Mikael Kubista", "Stefania Gelmini"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112293.g002", "stats"=>{"downloads"=>0, "page_views"=>57, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_Transcript_levels_of_FOS_and_IL1B_in_individual_and_pools_samples_/1234953", "title"=>"Relative Transcript levels of FOS and IL1B in individual and pools samples.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-10 03:44:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/1786599"], "description"=>"<p>Overall distribution of FOS and IL8 according to blood collection tube (3A and 3C, respectively) and to storage temperature/collection tube in RNA D (3B and 3D, respectively). The box horizontal sides identify the 25th and 75th centile, the horizontal line inside the box the median, the two whiskers correspond to minimum and maximum, and the dashed line indicates the T<sub>0</sub> value zero.</p>", "links"=>[], "tags"=>["TNFRSF 10c Analysis", "gene expression results", "SPIDIA", "eqa", "blood collection tubes", "proficiency blood specimens", "gapdh", "fos", "ec", "gene expression analysis", "fosb", "blood collection process", "1b", "gene expression levels", "blood collection tube type", "External Quality Assessments", "il", "RNA stabilizer"], "article_id"=>1234954, "categories"=>["Uncategorised"], "users"=>["Francesca Malentacchi", "Mario Pazzagli", "Lisa Simi", "Claudio Orlando", "Ralf Wyrich", "Kalle Günther", "Paolo Verderio", "Sara Pizzamiglio", "Chiara Maura Ciniselli", "Hui Zhang", "Vlasta Korenková", "Lynne Rainen", "Tzachi Bar", "Mikael Kubista", "Stefania Gelmini"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112293.g003", "stats"=>{"downloads"=>1, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Blood_collection_tube_and_or_storage_temperature_and_Gene_Expression_/1234954", "title"=>"Blood collection tube and/or storage temperature and Gene Expression.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-10 03:44:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/1786600"], "description"=>"a<p><b>all “in control” or “warning”</b>: labs with all parameters in control or warning, without missing;</p>b<p><b>one “out of control” and/or one or more “missing”</b>: labs with only one out of control (D1: n = 16, D2: n = 13); labs with only one missing (D1: n = 0, D2: n = 1) or only more than one missing (D1: n = 0, D2: n = 1); labs with one out of control and one missing (D1: n = 0, D2: n = 1); labs with one out of control and more than one missing (D1: n = 0, D2: n = 0);</p>c<p><b>two or more “out of control” with or without “missing</b>”: labs with two out of control with at least one missing (D1: n = 2, D2: n = 3) or without missing (D1: n = 5, D2: n = 6); labs with more than two out of control with at least one missing (D1: n = 3, D2: n = 3) or without missing (D1: n = 5, D2: n = 3).</p><p>Classification of the proficiency of the laboratories.</p>", "links"=>[], "tags"=>["TNFRSF 10c Analysis", "gene expression results", "SPIDIA", "eqa", "blood collection tubes", "proficiency blood specimens", "gapdh", "fos", "ec", "gene expression analysis", "fosb", "blood collection process", "1b", "gene expression levels", "blood collection tube type", "External Quality Assessments", "il", "RNA stabilizer"], "article_id"=>1234955, "categories"=>["Uncategorised"], "users"=>["Francesca Malentacchi", "Mario Pazzagli", "Lisa Simi", "Claudio Orlando", "Ralf Wyrich", "Kalle Günther", "Paolo Verderio", "Sara Pizzamiglio", "Chiara Maura Ciniselli", "Hui Zhang", "Vlasta Korenková", "Lynne Rainen", "Tzachi Bar", "Mikael Kubista", "Stefania Gelmini"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112293.t001", "stats"=>{"downloads"=>1, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Classification_of_the_proficiency_of_the_laboratories_/1234955", "title"=>"Classification of the proficiency of the laboratories.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-10 03:44:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/1786601", "https://ndownloader.figshare.com/files/1786602", "https://ndownloader.figshare.com/files/1786603", "https://ndownloader.figshare.com/files/1786604", "https://ndownloader.figshare.com/files/1786605", "https://ndownloader.figshare.com/files/1786606", "https://ndownloader.figshare.com/files/1786607", "https://ndownloader.figshare.com/files/1786608", "https://ndownloader.figshare.com/files/1786609"], "description"=>"<div><p>One purpose of the EC funded project, SPIDIA, is to develop evidence-based quality guidelines for the pre-analytical handling of blood samples for RNA molecular testing. To this end, two pan-European External Quality Assessments (EQAs) were implemented. Here we report the results of the second SPIDIA-RNA EQA. This second study included modifications in the protocol related to the blood collection process, the shipping conditions and pre-analytical specimen handling for participants. Participating laboratories received two identical proficiency blood specimens collected in tubes with or without an RNA stabilizer. For pre-defined specimen storage times and temperatures, laboratories were asked to perform RNA extraction from whole blood according to their usual procedure and to return extracted RNA to the SPIDIA facility for further analysis. These RNA samples were evaluated for purity, yield, integrity, stability, presence of interfering substances, and gene expression levels for the validated markers of RNA stability: FOS, IL1B, IL8, GAPDH, FOSB and TNFRSF10c. Analysis of the gene expression results of FOS, IL8, FOSB, and TNFRSF10c, however, indicated that the levels of these transcripts were significantly affected by blood collection tube type and storage temperature. These results demonstrated that only blood collection tubes containing a cellular RNA stabilizer allowed reliable gene expression analysis within 48 h from blood collection for all the genes investigated. The results of these two EQAs have been proposed for use in the development of a Technical Specification by the European Committee for Standardization.</p></div>", "links"=>[], "tags"=>["TNFRSF 10c Analysis", "gene expression results", "SPIDIA", "eqa", "blood collection tubes", "proficiency blood specimens", "gapdh", "fos", "ec", "gene expression analysis", "fosb", "blood collection process", "1b", "gene expression levels", "blood collection tube type", "External Quality Assessments", "il", "RNA stabilizer"], "article_id"=>1234956, "categories"=>["Uncategorised"], "users"=>["Francesca Malentacchi", "Mario Pazzagli", "Lisa Simi", "Claudio Orlando", "Ralf Wyrich", "Kalle Günther", "Paolo Verderio", "Sara Pizzamiglio", "Chiara Maura Ciniselli", "Hui Zhang", "Vlasta Korenková", "Lynne Rainen", "Tzachi Bar", "Mikael Kubista", "Stefania Gelmini"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0112293.s001", "https://dx.doi.org/10.1371/journal.pone.0112293.s002", "https://dx.doi.org/10.1371/journal.pone.0112293.s003", "https://dx.doi.org/10.1371/journal.pone.0112293.s004", "https://dx.doi.org/10.1371/journal.pone.0112293.s005", "https://dx.doi.org/10.1371/journal.pone.0112293.s006", "https://dx.doi.org/10.1371/journal.pone.0112293.s007", "https://dx.doi.org/10.1371/journal.pone.0112293.s008", "https://dx.doi.org/10.1371/journal.pone.0112293.s009"], "stats"=>{"downloads"=>18, "page_views"=>45, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SPIDIA_RNA_Second_External_Quality_Assessment_for_the_Pre_Analytical_Phase_of_Blood_Samples_Used_for_RNA_Based_Analyses_/1234956", "title"=>"SPIDIA-RNA: Second External Quality Assessment for the Pre-Analytical Phase of Blood Samples Used for RNA Based Analyses", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-11-10 03:44:34"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"21", "full-text"=>"19", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"29", "full-text"=>"19", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"9", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"20", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[267]}, {"subject_area"=>"/Biology and life sciences/Evolutionary biology", "average_usage"=>[333]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[280]}, {"subject_area"=>"/Biology and life sciences/Population biology", "average_usage"=>[312, 475]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[266]}, {"subject_area"=>"/Medicine and health sciences/Hematology", "average_usage"=>[256]}]}
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