Hyaluronidase Modulates Inflammatory Response and Accelerates the Cutaneous Wound Healing
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{"title"=>"Hyaluronidase modulates inflammatory response and accelerates the cutaneous wound healing", "type"=>"journal", "authors"=>[{"first_name"=>"Marcio", "last_name"=>"Fronza", "scopus_author_id"=>"10138852200"}, {"first_name"=>"Guilherme F.", "last_name"=>"Caetano", "scopus_author_id"=>"56418305400"}, {"first_name"=>"Marcel N.", "last_name"=>"Leite", "scopus_author_id"=>"56411701400"}, {"first_name"=>"Claudia S.", "last_name"=>"Bitencourt", "scopus_author_id"=>"36338661900"}, {"first_name"=>"Francisco W.G.", "last_name"=>"Paula-Silva", "scopus_author_id"=>"30267829400"}, {"first_name"=>"Thiago A.M.", "last_name"=>"Andrade", "scopus_author_id"=>"53982637600"}, {"first_name"=>"Marco A.C.", "last_name"=>"Frade", "scopus_author_id"=>"6505865815"}, {"first_name"=>"Irmgard", "last_name"=>"Merfort", "scopus_author_id"=>"7006557061"}, {"first_name"=>"Lúcia H.", "last_name"=>"Faccioli", "scopus_author_id"=>"7003377624"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84911946689", "doi"=>"10.1371/journal.pone.0112297", "pui"=>"600506670", "pmid"=>"25393024", "scopus"=>"2-s2.0-84911946689", "issn"=>"19326203"}, "id"=>"613b10e7-2a97-39e1-898e-cccb9ab5c201", "abstract"=>"Hyaluronidases are enzymes that degrade hyaluronan an important constituent of the extracellular matrix. They have been used as a spreading agent, improving the absorption of drugs and facilitating the subcutaneous infusion of fluids. Here, we investigated the influence of bovine testes hyaluronidase (HYAL) during cutaneous wound healing in in vitro and in vivo assays. We demonstrated in the wound scratch assay that HYAL increased the migration and proliferation of fibroblasts in vitro at low concentration, e.g. 0.1 U HYAL enhanced the cell number by 20%. HYAL presented faster and higher reepithelialization in in vivo full-thickness excisional wounds generated on adult Wistar rats back skin already in the early phase at 2nd day post operatory compared to vehicle-control group. Wound closured area observed in the 16 U and 32 U HYAL treated rats reached 38% and 46% compared to 19% in the controls, respectively. Histological and biochemical analyses supported the clinical observations and showed that HYAL treated wounds exhibited increased granulation tissue, diminished edema formation and regulated the inflammatory response by modulating the release of pro and anti-inflammatory cytokines, growth factor and eicosanoids mediators. Moreover, HYAL increased gene expression of peroxisome proliferator-activated receptors (PPAR) γ and PPAR β/δ, the collagen content in the early stages of healing processes as well as angiogenesis. Altogether these data revealed that HYAL accelerates wound healing processes and might be beneficial for treating wound disorders.", "link"=>"http://www.mendeley.com/research/hyaluronidase-modulates-inflammatory-response-accelerates-cutaneous-wound-healing-1", "reader_count"=>42, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>4, "Researcher"=>8, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>4, "Other"=>2, "Student > Master"=>7, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>4, "Researcher"=>8, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>4, "Other"=>2, "Student > Master"=>7, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Engineering"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Nursing and Health Professions"=>2, "Materials Science"=>2, "Medicine and Dentistry"=>13, "Agricultural and Biological Sciences"=>5, "Pharmacology, Toxicology and Pharmaceutical Science"=>7, "Chemistry"=>2, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Materials Science"=>{"Materials Science"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>13}, "Chemistry"=>{"Chemistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>5}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>4}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>7}}, "group_count"=>2}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1793581"], "description"=>"<p>(A) Representative photographs taken from the 150 mm diameter full-thickness wounds of the Wistar rats. Macroscopic changes in skin wound sites induced by topical application of vehicle, HYAL 16 U and HYAL 32 U at day 0 (picture taken immediately after injury) 2, 5, 7, 10, 14 and day 21 are shown. (B) Rate of wound closure induced by topical application of vehicle (control group), HYAL 16 U and HYAL 32 U at day 2, 5, 7, 10, 14 and day 21 are given. Data are expressed as percentage of reduction area from the original wound size (day zero). Values are mean ± SEM (n = 8 to 16 wounds/group), *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 compared to control group by two-way-ANOVA.</p>", "links"=>[], "tags"=>["adult Wistar rats", "day post operatory", "cell number", "Wound closured area", "vivo assays", "testes hyaluronidase", "gene expression", "growth factor", "edema formation", "32 U HYAL", "collagen content", "ppar", "Hyaluronidase Modulates", "granulation tissue", "cutaneous wound healing", "subcutaneous infusion", "Extracellular matrix", "0.1 U HYAL", "wound scratch assay", "wound disorders", "Cutaneous Wound Healing Hyaluronidases", "healing processes", "wound healing processes", "eicosanoids mediators", "16 U"], "article_id"=>1240816, "categories"=>["Biological Sciences"], "users"=>["Marcio Fronza", "Guilherme F. Caetano", "Marcel N. Leite", "Claudia S. Bitencourt", "Francisco W. G. Paula-Silva", "Thiago A. M. Andrade", "Marco A. C. Frade", "Irmgard Merfort", "Lúcia H. Faccioli"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112297.g001", "stats"=>{"downloads"=>3, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Topical_application_of_HYAL_accelerates_wound_closure_in_full_thickness_excisional_wounds_/1240816", "title"=>"Topical application of HYAL accelerates wound closure in full-thickness excisional wounds.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-13 04:08:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1793585"], "description"=>"<p>Animals were topically treated either with vehicle (control group) or HYAL 16 U daily. Paraffin-wound sections were stained with HE to evaluate the inflammatory infiltrate response by image analysis. (A) The sections were photographed at 400x. The ImageJ software was used to count the inflammatory cells in wound tissue specimens at day 2, 7, 14 and 21 post wounding in at least ten random optic fields per group. (B) Histogram of a quantitative analysis of inflammatory infiltrate counted. (C) Tissue neutrophil accumulation determined by MPO levels in wound biopsies. (D) The total protein content was measured according to Coomassie assay. Values represent mean ± SEM (n = 8 wounds/group), *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 compared to control group by one-way-ANOVA.</p>", "links"=>[], "tags"=>["adult Wistar rats", "day post operatory", "cell number", "Wound closured area", "vivo assays", "testes hyaluronidase", "gene expression", "growth factor", "edema formation", "32 U HYAL", "collagen content", "ppar", "Hyaluronidase Modulates", "granulation tissue", "cutaneous wound healing", "subcutaneous infusion", "Extracellular matrix", "0.1 U HYAL", "wound scratch assay", "wound disorders", "Cutaneous Wound Healing Hyaluronidases", "healing processes", "wound healing processes", "eicosanoids mediators", "16 U"], "article_id"=>1240818, "categories"=>["Biological Sciences"], "users"=>["Marcio Fronza", "Guilherme F. Caetano", "Marcel N. Leite", "Claudia S. Bitencourt", "Francisco W. G. Paula-Silva", "Thiago A. M. Andrade", "Marco A. C. Frade", "Irmgard Merfort", "Lúcia H. Faccioli"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112297.g002", "stats"=>{"downloads"=>4, "page_views"=>39, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_HYAL_affects_cellular_recruitment_and_edema_formation_/1240818", "title"=>"HYAL affects cellular recruitment and edema formation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-13 04:08:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1793586"], "description"=>"<p>Homogenates were prepared from the wound biopsies obtained from animals at day 0, 2, 7, 14 and 21 treated with 16 U HYAL or with vehicle-control. (A) IL1-α, (B) TNF-α, (C) IL-4, (D) IL-10, (E) TGF-β, (F) PGE<sub>2</sub>, (G) LTB<sub>4</sub> and (H) PGD<sub>2</sub> were assayed by ELISA. Data are means ± SEM (n = 8 wounds/group), *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 compared to control group by one-way-ANOVA.</p>", "links"=>[], "tags"=>["adult Wistar rats", "day post operatory", "cell number", "Wound closured area", "vivo assays", "testes hyaluronidase", "gene expression", "growth factor", "edema formation", "32 U HYAL", "collagen content", "ppar", "Hyaluronidase Modulates", "granulation tissue", "cutaneous wound healing", "subcutaneous infusion", "Extracellular matrix", "0.1 U HYAL", "wound scratch assay", "wound disorders", "Cutaneous Wound Healing Hyaluronidases", "healing processes", "wound healing processes", "eicosanoids mediators", "16 U"], "article_id"=>1240819, "categories"=>["Biological Sciences"], "users"=>["Marcio Fronza", "Guilherme F. Caetano", "Marcel N. Leite", "Claudia S. Bitencourt", "Francisco W. G. Paula-Silva", "Thiago A. M. Andrade", "Marco A. C. Frade", "Irmgard Merfort", "Lúcia H. Faccioli"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112297.g003", "stats"=>{"downloads"=>2, "page_views"=>32, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_HYAL_modulates_cytokines_and_induces_eicosanoid_generation_in_the_skin_wound_biopsies_/1240819", "title"=>"HYAL modulates cytokines and induces eicosanoid generation in the skin wound biopsies.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-13 04:08:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1793590"], "description"=>"<p>(A) Representative photomicrograph of wounds tissue sections stained with picrosirius red staining (200x), note the collagen intensity and disposition of fibers (red). (B) Collagen content measured by digital densitometry is shown as a result of collagen content in each specimen in percentage. (C) Determination of wound hydroxyproline content as an indicator of collagen levels. µg of hydroxyproline/mg of dry wound specimen content was measured at day zero, 2, 7, 14 and 21 after injury. Data represent means ± SEM (n = 8 wounds/group), *<i>P</i><0.05, **<i>P</i><0.01 compared to control group by one-way-ANOVA.</p>", "links"=>[], "tags"=>["adult Wistar rats", "day post operatory", "cell number", "Wound closured area", "vivo assays", "testes hyaluronidase", "gene expression", "growth factor", "edema formation", "32 U HYAL", "collagen content", "ppar", "Hyaluronidase Modulates", "granulation tissue", "cutaneous wound healing", "subcutaneous infusion", "Extracellular matrix", "0.1 U HYAL", "wound scratch assay", "wound disorders", "Cutaneous Wound Healing Hyaluronidases", "healing processes", "wound healing processes", "eicosanoids mediators", "16 U"], "article_id"=>1240820, "categories"=>["Biological Sciences"], "users"=>["Marcio Fronza", "Guilherme F. Caetano", "Marcel N. Leite", "Claudia S. Bitencourt", "Francisco W. G. Paula-Silva", "Thiago A. M. Andrade", "Marco A. C. Frade", "Irmgard Merfort", "Lúcia H. Faccioli"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112297.g004", "stats"=>{"downloads"=>2, "page_views"=>42, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Collagen_accumulation_in_wound_areas_of_HYAL_and_vehicle_control_treated_rats_at_day_2_7_14_and_21_after_wounding_/1240820", "title"=>"Collagen accumulation in wound areas of HYAL and vehicle-control treated rats at day 2, 7, 14 and 21 after wounding.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-13 04:08:31"}

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