Involvement of TNF-α Converting Enzyme in the Development of Psoriasis-Like Lesions in a Mouse Model
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Involvement of TNF- α converting enzyme in the development of psoriasis-like lesions in a mouse model", "type"=>"journal", "authors"=>[{"first_name"=>"Kenji", "last_name"=>"Sato", "scopus_author_id"=>"55624480849"}, {"first_name"=>"Mikiro", "last_name"=>"Takaishi", "scopus_author_id"=>"7003299271"}, {"first_name"=>"Shota", "last_name"=>"Tokuoka", "scopus_author_id"=>"7005208939"}, {"first_name"=>"Shigetoshi", "last_name"=>"Sano", "scopus_author_id"=>"55499154600"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84911365869", "pui"=>"600460901", "doi"=>"10.1371/journal.pone.0112408", "sgr"=>"84911365869", "pmid"=>"25384035"}, "id"=>"5760dde1-aa73-3ea2-a396-9c5220c36ba0", "abstract"=>"TNF-α plays a crucial role in psoriasis; therefore, TNF inhibition has become a gold standard for the treatment of psoriasis. TNF-α is processed from a membrane-bound form by TNF-α converting enzyme (TACE) to soluble form, which exerts a number of biological activities. EGF receptor (EGFR) ligands, including heparin-binding EGF-like growth factor (HB-EGF), amphiregulin and transforming growth factor (TGF)-α are also TACE substrates and are psoriasis-associated growth factors. Vascular endothelial growth factor (VEGF), one of the downstream molecules of EGFR and TNF signaling, plays a key role in angiogenesis for developing psoriasis. In the present study, to assess the possible role of TACE in the pathogenesis of psoriasis, we investigated the involvement of TACE in TPA-induced psoriasis-like lesions in K5.Stat3C mice, which represent a mouse model of psoriasis. In this mouse model, TNF-α, amphiregulin, HB-EGF and TGF-α were significantly up-regulated in the skin lesions, similar to human psoriasis. Treatment of K5.Stat3C mice with TNF-α or EGFR inhibitors attenuated the skin lesions, suggesting the roles of TACE substrates in psoriasis. Furthermore, the skin lesions of K5.Stat3C mice showed down-regulation of tissue inhibitor of metalloproteinase-3, an endogenous inhibitor of TACE, and an increase in soluble TNF-α. A TACE inhibitor abrogated EGFR ligand-dependent keratinocyte proliferation and VEGF production in vitro, suggesting that TACE was involved in both epidermal hyperplasia and angiogenesis during psoriasis development. These results strongly suggest that TACE contributes to the development of psoriatic lesions through releasing two kinds of psoriasis mediators, TNF-α and EGFR ligands. Therefore, TACE could be a potential therapeutic target for the treatment of psoriasis.", "link"=>"http://www.mendeley.com/research/involvement-tnf-%CE%B1-converting-enzyme-development-psoriasislike-lesions-mouse-model", "reader_count"=>23, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>9, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>4, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>9, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>4, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Agricultural and Biological Sciences"=>9, "Medicine and Dentistry"=>4, "Arts and Humanities"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>3, "Chemistry"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Unspecified"=>{"Unspecified"=>4}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>3}, "Arts and Humanities"=>{"Arts and Humanities"=>1}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84911365869"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84911365869?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84911365869&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84911365869&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84911365869", "dc:identifier"=>"SCOPUS_ID:84911365869", "eid"=>"2-s2.0-84911365869", "dc:title"=>"Involvement of TNF- α converting enzyme in the development of psoriasis-like lesions in a mouse model", "dc:creator"=>"Sato K.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"11", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-11-10", "prism:coverDisplayDate"=>"10 November 2014", "prism:doi"=>"10.1371/journal.pone.0112408", "citedby-count"=>"12", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Kochi University", "affiliation-city"=>"Kochi", "affiliation-country"=>"Japan"}, {"@_fa"=>"true", "affilname"=>"Kaken Pharmaceutical Co., Ltd.", "affiliation-city"=>"Kyoto", "affiliation-country"=>"Japan"}], "pubmed-id"=>"25384035", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e112408", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0112408", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"91", "xml_views"=>"9", "html_views"=>"251"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"27", "xml_views"=>"2", "html_views"=>"87"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"24", "xml_views"=>"2", "html_views"=>"131"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"54"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"34"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"23"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"36"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"7"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1786108", "https://ndownloader.figshare.com/files/1786109", "https://ndownloader.figshare.com/files/1786110", "https://ndownloader.figshare.com/files/1786111"], "description"=>"<div><p>TNF-α plays a crucial role in psoriasis; therefore, TNF inhibition has become a gold standard for the treatment of psoriasis. TNF-α is processed from a membrane-bound form by TNF-α converting enzyme (TACE) to soluble form, which exerts a number of biological activities. EGF receptor (EGFR) ligands, including heparin-binding EGF-like growth factor (HB-EGF), amphiregulin and transforming growth factor (TGF)-α are also TACE substrates and are psoriasis-associated growth factors. Vascular endothelial growth factor (VEGF), one of the downstream molecules of EGFR and TNF signaling, plays a key role in angiogenesis for developing psoriasis. In the present study, to assess the possible role of TACE in the pathogenesis of psoriasis, we investigated the involvement of TACE in TPA-induced psoriasis-like lesions in K5.Stat3C mice, which represent a mouse model of psoriasis. In this mouse model, TNF-α, amphiregulin, HB-EGF and TGF-α were significantly up-regulated in the skin lesions, similar to human psoriasis. Treatment of K5.Stat3C mice with TNF-α or EGFR inhibitors attenuated the skin lesions, suggesting the roles of TACE substrates in psoriasis. Furthermore, the skin lesions of K5.Stat3C mice showed down-regulation of tissue inhibitor of metalloproteinase-3, an endogenous inhibitor of TACE, and an increase in soluble TNF-α. A TACE inhibitor abrogated EGFR ligand-dependent keratinocyte proliferation and VEGF production in vitro, suggesting that TACE was involved in both epidermal hyperplasia and angiogenesis during psoriasis development. These results strongly suggest that TACE contributes to the development of psoriatic lesions through releasing two kinds of psoriasis mediators, TNF-α and EGFR ligands. Therefore, TACE could be a potential therapeutic target for the treatment of psoriasis.</p></div>", "links"=>[], "tags"=>["vegf", "tgf", "EGFR inhibitors attenuated", "K 5.Stat mice", "tnf", "growth factor", "psoriasis", "Mouse Model", "role", "skin lesions", "TACE substrates"], "article_id"=>1234560, "categories"=>["Biological Sciences"], "users"=>["Kenji Sato", "Mikiro Takaishi", "Shota Tokuoka", "Shigetoshi Sano"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0112408.s001", "https://dx.doi.org/10.1371/journal.pone.0112408.s002", "https://dx.doi.org/10.1371/journal.pone.0112408.s003", "https://dx.doi.org/10.1371/journal.pone.0112408.s004"], "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Involvement_of_TNF_945_Converting_Enzyme_in_the_Development_of_Psoriasis_Like_Lesions_in_a_Mouse_Model_/1234560", "title"=>"Involvement of TNF-α Converting Enzyme in the Development of Psoriasis-Like Lesions in a Mouse Model", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-11-10 03:08:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1786103"], "description"=>"<p>(A–C), Representative histology and immunohistochemistry of human skins (A), ear skins in K5.Stat3C mice (B), and wild-type mice (C). non-pso, non-psoriasis control; TPA, TPA-treated ear skins sampled at day 3; Hematoxylin and eosin staining (H&E, top panels), Immunohistochemical staining for TACE (middle panels) and TNF-α (bottom panels). Arrow, intraepidermal pustule of neutrophils. Bars = 100 µm (human), 50 µm (mouse). (D–F), epidermal thickness (D), TNF-α mRNA expression (E) and TNF-α protein levels (F) in the TPA-treated ear skins of K5.Stat3C mice (black bars) and wild-type mice (white bars). Data represent means ± SD of 3 to 8 mice. *<i>p</i><0.05, **<i>p</i><0.01, versus K5.Stat3C mice at day 0, <sup>†</sup><i>p</i><0.05, <sup>††</sup><i>p</i><0.01, versus wild-type mice at day 0, by Dunnett’s test.</p>", "links"=>[], "tags"=>["vegf", "tgf", "EGFR inhibitors attenuated", "K 5.Stat mice", "tnf", "growth factor", "psoriasis", "Mouse Model", "role", "skin lesions", "TACE substrates"], "article_id"=>1234555, "categories"=>["Biological Sciences"], "users"=>["Kenji Sato", "Mikiro Takaishi", "Shota Tokuoka", "Shigetoshi Sano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112408.g001", "stats"=>{"downloads"=>6, "page_views"=>296, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_TACE_and_TNF_945_in_the_development_of_psoriasis_like_skin_lesions_in_K5_Stat3C_mice_/1234555", "title"=>"Expression of TACE and TNF-α in the development of psoriasis-like skin lesions in K5.Stat3C mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-10 03:08:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1786104"], "description"=>"<p>(A), Ear thickness (Δmm) ± SD following topical TPA in mice treated with control IgG (n = 6, squares) and etanercept (n = 6, triangles). *<i>p</i><0.05, **<i>p</i><0.01, versus control IgG, by Student’s <i>t</i>-test. (B), Representative histology of ear skins from K5.Stat3C mice treated with control IgG (top) and etanercept (bottom). H&E staining. Bar = 100 µm. (C–E), Inhibitory effect of etanercept (Etn) on TPA-induced epidermal hyperplasia (C), gene expression of IL-17A (D), and IL-12/23p40 (E) in ear skins. Data represent means ± SD of 3 to 6 mice. *<i>p</i><0.05, **<i>p</i><0.01, versus control IgG, by Student’s <i>t</i>-test.</p>", "links"=>[], "tags"=>["vegf", "tgf", "EGFR inhibitors attenuated", "K 5.Stat mice", "tnf", "growth factor", "psoriasis", "Mouse Model", "role", "skin lesions", "TACE substrates"], "article_id"=>1234556, "categories"=>["Biological Sciences"], "users"=>["Kenji Sato", "Mikiro Takaishi", "Shota Tokuoka", "Shigetoshi Sano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112408.g002", "stats"=>{"downloads"=>1, "page_views"=>39, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Involvement_of_TNF_945_in_the_development_of_psoriasis_like_skin_lesions_in_K5_Stat3C_mice_/1234556", "title"=>"Involvement of TNF-α in the development of psoriasis-like skin lesions in K5.Stat3C mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-10 03:08:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1786105"], "description"=>"<p>(A), Gene expression of amphiregulin, HB-EGF and TGF-α in the TPA-treated ear skins of K5.Stat3C mice (black bars) and wild-type mice (white bars). Data represent means ± SD of 3 to 8 mice. *<i>p</i><0.05, **<i>p</i><0.01, versus K5.Stat3C mice at day 0, <sup>†</sup><i>p</i><0.05, <sup>††</sup><i>p</i><0.01, versus wild-type mice at day 0, by Dunnett’s test. (B), Representative histology of ear skins from K5.Stat3C mice treated with vehicle alone (top) and AG1478 (bottom). Bars = 100 µm. (C), Suppression of TPA-induced epidermal hyperplasia by treatment with AG1478 (AG) at day 3. Data represent means ± SD of 3 to 5 mice, *<i>p</i><0.05 versus vehicle alone (V) by Student’s <i>t</i>-test.</p>", "links"=>[], "tags"=>["vegf", "tgf", "EGFR inhibitors attenuated", "K 5.Stat mice", "tnf", "growth factor", "psoriasis", "Mouse Model", "role", "skin lesions", "TACE substrates"], "article_id"=>1234557, "categories"=>["Biological Sciences"], "users"=>["Kenji Sato", "Mikiro Takaishi", "Shota Tokuoka", "Shigetoshi Sano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112408.g003", "stats"=>{"downloads"=>1, "page_views"=>71, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Involvement_of_EGFR_signaling_in_the_development_of_psoriasis_like_skin_lesions_in_K5_Stat3C_mice_/1234557", "title"=>"Involvement of EGFR signaling in the development of psoriasis-like skin lesions in K5.Stat3C mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-10 03:08:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1786106"], "description"=>"<p>(A), Gene expression of TIMP-3 in the TPA-treated ear skins of K5.Stat3C mice (black bars) and wild-type mice (white bars). Data represent means ± SD of 3 to 8 mice. **<i>p</i><0.01, versus K5.Stat3C mice at day 0, <sup>††</sup><i>p</i><0.01, versus wild-type mice at day 0, by Dunnett’s test. (B), Western blot analysis of TIMP-3 in ear skins of K5.Stat3C mice collected before TPA application (0) or 6 h (0.25) after TPA application. (C), Western blot analysis of soluble TNF-α (sTNF-α), which is around 17 kDa of molecular size, in the lysates from ear skins of K5.Stat3C mice compared with wild-type mice. The ear skins were collected before TPA application (0) or at 6 h (0.25) and 1 day (1) after TPA application. C, Control of soluble TNF-α. (D), Enzymatic activity of TACE in ear skins of K5.Stat3C mice versus wild-type mice; untreated control (white bars) and TPA-treated at 6h of the second TPA application (black bars). Enzymatic activity in skins was indicated as ratio to that in untreated wild-type skins. Data represent means ± SD of 4 to 5 mice, **<i>p</i><0.01; n.s., not significant by Tukey-Kramer’s test.</p>", "links"=>[], "tags"=>["vegf", "tgf", "EGFR inhibitors attenuated", "K 5.Stat mice", "tnf", "growth factor", "psoriasis", "Mouse Model", "role", "skin lesions", "TACE substrates"], "article_id"=>1234558, "categories"=>["Biological Sciences"], "users"=>["Kenji Sato", "Mikiro Takaishi", "Shota Tokuoka", "Shigetoshi Sano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112408.g004", "stats"=>{"downloads"=>0, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Down_regulation_of_TIMP_3_an_endogenous_TACE_inhibitor_and_TACE_enzymatic_activity_in_the_development_of_psoriasis_like_skin_lesions_/1234558", "title"=>"Down-regulation of TIMP-3, an endogenous TACE inhibitor, and TACE enzymatic activity in the development of psoriasis-like skin lesions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-10 03:08:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/1786107"], "description"=>"<p>(A–C), TNF-α proteins in the 24 h culture supernatants of cells pretreated with or without TAPI-1 (TAPI) at the indicated concentrations. LPS-stimulated bone marrow derived dendritic cells (BMDCs) from wild-type FVB/N mice (A), LPS-stimulated thioglycolate-elicited peritoneal macrophages from wild-type FVB/N mice (B), and TPA-stimulated primary keratinocytes from K5.Stat3C newborn mice (C). Cells were pretreated with or without TAPI-1 (TAPI) for 30 min prior to stimulation. (D), Production of amphiregulin from TPA-treated primary keratinocytes of K5.Stat3C newborn mice for 24 h. Cells were pretreated with or without TAPI-1 (TAPI) at the indicated concentrations for 30 min prior to stimulation. U, unstimulated control. V, vehicle alone. Data represent means ± SD of triplicate wells. *<i>p</i><0.05, **<i>p</i><0.01, versus vehicle alone by Dunnett’s test. (E), Keratinocyte proliferation evaluated by MTS assay with absorbance at 490 nm. Cells were untreated or treated with TAPI-1 (100 µmol/l) (T) and cultured in the absence or presence of HB-EGF (HB), amphiregulin (Areg), AG1478 (AG) or etanercept (Etn) for 3 days. (F), VEGF production from primary keratinocytes. Cells were stimulated with TPA for 24 h treated with or without reagents as described. Data represent means ± SD from triplicate wells. *<i>p</i><0.05, **<i>p</i><0.01 versus vehicle alone, <sup>††</sup><i>p</i><0.01 versus TAPI-1 alone by Tukey-Kramer’s test.</p>", "links"=>[], "tags"=>["vegf", "tgf", "EGFR inhibitors attenuated", "K 5.Stat mice", "tnf", "growth factor", "psoriasis", "Mouse Model", "role", "skin lesions", "TACE substrates"], "article_id"=>1234559, "categories"=>["Biological Sciences"], "users"=>["Kenji Sato", "Mikiro Takaishi", "Shota Tokuoka", "Shigetoshi Sano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112408.g005", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Contribution_of_TACE_to_the_production_of_soluble_TNF_945_and_EGFR_ligands_from_murine_cells_/1234559", "title"=>"Contribution of TACE to the production of soluble TNF-α and EGFR ligands from murine cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-10 03:08:29"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"28", "full-text"=>"27", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"28", "full-text"=>"25", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"31", "full-text"=>"42", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"21", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"26", "full-text"=>"31", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"25", "full-text"=>"30", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"26", "full-text"=>"19", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"25", "full-text"=>"25", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"45", "full-text"=>"40", "pdf"=>"24", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"35", "full-text"=>"34", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"54", "full-text"=>"42", "pdf"=>"33", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"41", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"22", "full-text"=>"15", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"19", "full-text"=>"28", "pdf"=>"26", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"22", "full-text"=>"26", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"19", "full-text"=>"16", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"17", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"13", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Immunology", "average_usage"=>[293]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[280]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[266]}, {"subject_area"=>"/Medicine and health sciences/Endocrinology", "average_usage"=>[282, 453]}, {"subject_area"=>"/Medicine and health sciences/Physiology", "average_usage"=>[278]}]}
Loading … Spinner
There are currently no alerts