Morphological Changes of Paulownia Seedlings Infected Phytoplasmas Reveal the Genes Associated with Witches' Broom through AFLP and MSAP
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{"title"=>"Morphological changes of Paulownia seedlings infected phytoplasmas reveal the genes associated with witches' broom through AFLP and MSAP", "type"=>"journal", "authors"=>[{"first_name"=>"Xibing", "last_name"=>"Cao", "scopus_author_id"=>"56043369400"}, {"first_name"=>"Guoqiang", "last_name"=>"Fan", "scopus_author_id"=>"56011438000"}, {"first_name"=>"Zhenli", "last_name"=>"Zhao", "scopus_author_id"=>"56042802500"}, {"first_name"=>"Minjie", "last_name"=>"Deng", "scopus_author_id"=>"56044171000"}, {"first_name"=>"Yanpeng", "last_name"=>"Dong", "scopus_author_id"=>"56043882900"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pui"=>"600595804", "doi"=>"10.1371/journal.pone.0112533", "sgr"=>"84913582466", "scopus"=>"2-s2.0-84913582466"}, "id"=>"0edacb77-b5ca-3f74-84ff-a34bd762340c", "link"=>"http://www.mendeley.com/research/morphological-changes-paulownia-seedlings-infected-phytoplasmas-reveal-genes-associated-witches-broo", "reader_count"=>4, "reader_count_by_academic_status"=>{"Researcher"=>2, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>2, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Agricultural and Biological Sciences"=>3, "Unspecified"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"Mexico"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1807622"], "description"=>"<p>1: PS; 2: PS-20; 3: PS-60; 4: PS-100; 5: HS; 6: ddH<sub>2</sub>O; M: DNA Marker.</p>", "links"=>[], "tags"=>["MSAP Paulownia witches", "Paulownia genomic DNA sequence", "fragment length polymorphism", "phytoplasma", "pattern", "methyl methane sulfonate", "PaWB seedlings", "Paulownia Seedlings Infected Phytoplasmas Reveal", "DNA methylation levels", "pcr", "aflp", "mms"], "article_id"=>1252352, "categories"=>["Biological Sciences"], "users"=>["Xibing Cao", "Guoqiang Fan", "Zhenli Zhao", "Minjie Deng", "Yanpeng Dong"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112533.g003", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_16S_rDNA_amplification_in_PaWB_seedlings_with_MMS_treatment_/1252352", "title"=>"16S rDNA amplification in PaWB seedlings with MMS treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-26 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/1807616"], "description"=>"<p>A: PaWB seedlings (PS); B: PS-20; C: PS-60; D: PS-100; E: Healthy seedlings (HS).</p>", "links"=>[], "tags"=>["MSAP Paulownia witches", "Paulownia genomic DNA sequence", "fragment length polymorphism", "phytoplasma", "pattern", "methyl methane sulfonate", "PaWB seedlings", "Paulownia Seedlings Infected Phytoplasmas Reveal", "DNA methylation levels", "pcr", "aflp", "mms"], "article_id"=>1252346, "categories"=>["Biological Sciences"], "users"=>["Xibing Cao", "Guoqiang Fan", "Zhenli Zhao", "Minjie Deng", "Yanpeng Dong"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112533.g002", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Changes_of_the_morphology_of_PaWB_seedlings_with_MMS_treatment_/1252346", "title"=>"Changes of the morphology of PaWB seedlings with MMS treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-26 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/1807681"], "description"=>"a<p>: H<sub>1</sub> and M<sub>1</sub>: bands digested by <i>Eco</i>RI/<i>Hpa</i>II (H) and <i>Eco</i>RI/<i>Msp</i>I (M) in PS; H<sub>x</sub> and M<sub>x</sub>: bands digested by H and M in MMS treated seedlings or HS; <u>C</u> and <u>CC</u>: cytosine methylation;</p>b<p>: 0–20: the number of DNA methylation patterns of PS-20 relative to PS; 0–60: the number of DNA methylation patterns of PS-60 relative to PS; 0–100: the number of DNA methylation patterns of PS-100 relative to PS; 0–HS: the number of DNA methylation patterns of HS relative to PS.</p><p>Changes of DNA methylation patterns in PaWB seedlings with MMS treatment.</p>", "links"=>[], "tags"=>["MSAP Paulownia witches", "Paulownia genomic DNA sequence", "fragment length polymorphism", "phytoplasma", "pattern", "methyl methane sulfonate", "PaWB seedlings", "Paulownia Seedlings Infected Phytoplasmas Reveal", "DNA methylation levels", "pcr", "aflp", "mms"], "article_id"=>1252391, "categories"=>["Biological Sciences"], "users"=>["Xibing Cao", "Guoqiang Fan", "Zhenli Zhao", "Minjie Deng", "Yanpeng Dong"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112533.t003", "stats"=>{"downloads"=>7, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Changes_of_DNA_methylation_patterns_in_PaWB_seedlings_with_MMS_treatment_/1252391", "title"=>"Changes of DNA methylation patterns in PaWB seedlings with MMS treatment.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-26 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/1807598"], "description"=>"<p>Comparison schemes of the different seedlings.</p>", "links"=>[], "tags"=>["MSAP Paulownia witches", "Paulownia genomic DNA sequence", "fragment length polymorphism", "phytoplasma", "pattern", "methyl methane sulfonate", "PaWB seedlings", "Paulownia Seedlings Infected Phytoplasmas Reveal", "DNA methylation levels", "pcr", "aflp", "mms"], "article_id"=>1252328, "categories"=>["Biological Sciences"], "users"=>["Xibing Cao", "Guoqiang Fan", "Zhenli Zhao", "Minjie Deng", "Yanpeng Dong"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112533.g001", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_schemes_of_the_different_seedlings_/1252328", "title"=>"Comparison schemes of the different seedlings.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-26 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/1807732", "https://ndownloader.figshare.com/files/1807733", "https://ndownloader.figshare.com/files/1807734", "https://ndownloader.figshare.com/files/1807735", "https://ndownloader.figshare.com/files/1807736"], "description"=>"<div><p>Paulownia witches' broom (PaWB) caused by phytoplasma might result in devastating damage to the growth and wood production of <i>Paulownia</i>. To study the effect of phytoplasma on DNA sequence and to discover the genes related to PaWB occurrence, DNA polymorphisms and DNA methylation levels and patterns in PaWB seedlings, the ones treated with various concentration of methyl methane sulfonate (MMS) and healthy seedlings were investigated with amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MSAP). Our results indicated that PaWB seedlings recovered a normal morphology, similar to healthy seedlings, after treatment with more than 20 mg·L<sup>−1</sup> MMS; Phytoplasma infection did not change the Paulownia genomic DNA sequence at AFLP level, but changed the global DNA methylation levels and patterns; Genes related to PaWB were discovered through MSAP and validated using quantitative real-time PCR (qRT-PCR). These results implied that changes of DNA methylation levels and patterns were closely related to the morphological changes of seedlings infected with phytoplasmas.</p></div>", "links"=>[], "tags"=>["MSAP Paulownia witches", "Paulownia genomic DNA sequence", "fragment length polymorphism", "phytoplasma", "pattern", "methyl methane sulfonate", "PaWB seedlings", "Paulownia Seedlings Infected Phytoplasmas Reveal", "DNA methylation levels", "pcr", "aflp", "mms"], "article_id"=>1252437, "categories"=>["Biological Sciences"], "users"=>["Xibing Cao", "Guoqiang Fan", "Zhenli Zhao", "Minjie Deng", "Yanpeng Dong"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0112533.s001", "https://dx.doi.org/10.1371/journal.pone.0112533.s002", "https://dx.doi.org/10.1371/journal.pone.0112533.s003", "https://dx.doi.org/10.1371/journal.pone.0112533.s004", "https://dx.doi.org/10.1371/journal.pone.0112533.s005"], "stats"=>{"downloads"=>21, "page_views"=>38, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Morphological_Changes_of_Paulownia_Seedlings_Infected_Phytoplasmas_Reveal_the_Genes_Associated_with_Witches_Broom_through_AFLP_and_MSAP_/1252437", "title"=>"Morphological Changes of Paulownia Seedlings Infected Phytoplasmas Reveal the Genes Associated with Witches' Broom through AFLP and MSAP", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-11-26 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/1807679"], "description"=>"a<p>: Total amplified bands  =  band of class I+ band of class II+ band of class III;</p>b<p>: total methylated bands  =  band of class II+ band of class III;</p>c<p>: methylation level (%)  =  (total methylated bands)/(total amplified bands)×100; HS: Healthy seedlings. The different letters within a column indicate significant difference, while the same letters within a column indicate no significant difference (<i>p</i><0.05).</p><p>Changes of DNA methylation levels in PaWB seedlings with MMS treatment.</p>", "links"=>[], "tags"=>["MSAP Paulownia witches", "Paulownia genomic DNA sequence", "fragment length polymorphism", "phytoplasma", "pattern", "methyl methane sulfonate", "PaWB seedlings", "Paulownia Seedlings Infected Phytoplasmas Reveal", "DNA methylation levels", "pcr", "aflp", "mms"], "article_id"=>1252390, "categories"=>["Biological Sciences"], "users"=>["Xibing Cao", "Guoqiang Fan", "Zhenli Zhao", "Minjie Deng", "Yanpeng Dong"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112533.t002", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Changes_of_DNA_methylation_levels_in_PaWB_seedlings_with_MMS_treatment_/1252390", "title"=>"Changes of DNA methylation levels in PaWB seedlings with MMS treatment.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-26 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/1807676"], "description"=>"<p>A: relative expression of chitin-inducible gibberellin-responsive protein (Chitin-P); B: relative expression of leucyl aminopeptidase (Leucyl-A); C: relative expression of cytochrome P450 76B6 (P450); D: relative expression of ring finger protein (RFP); E: relative expression of uncharacterized protein LOC100796964 (UP); F: relative expression of beta-hydroxyacyl-ACP dehydrase 1(Beta-ACP). 0–100: MMS concentrations (mg·L<sup>−1</sup>); HS: healthy seedlings. The different letters within a gene repression level indicate significant difference, while the same letters within a gene repression level indicate no significant differences (<i>p</i><0.05).</p>", "links"=>[], "tags"=>["MSAP Paulownia witches", "Paulownia genomic DNA sequence", "fragment length polymorphism", "phytoplasma", "pattern", "methyl methane sulfonate", "PaWB seedlings", "Paulownia Seedlings Infected Phytoplasmas Reveal", "DNA methylation levels", "pcr", "aflp", "mms"], "article_id"=>1252386, "categories"=>["Biological Sciences"], "users"=>["Xibing Cao", "Guoqiang Fan", "Zhenli Zhao", "Minjie Deng", "Yanpeng Dong"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112533.g005", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcriptional_analysis_of_6_Paulownia_genes_/1252386", "title"=>"Transcriptional analysis of 6 Paulownia genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-26 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/1807684"], "description"=>"a<p>: Total methylation bands  =  band of type A+ band of type B+ band of type C+ band of type D;</p>b<p>: type A, DNA methylation type, type A (%)  =  (band of type A)/(total methylation bands)×100;</p>c<p>: type B, DNA demethylation type, type B (%)  =  (band of type B)/(total methylation bands)×100;</p>d<p>: type C, uncertain DNA methylation type, type C (%)  =  (band of type C)/(total methylation bands)×100;</p>e<p>: type D, DNA methylation monomorphism, type D (%)  =  (band of type D)/(total methylation bands)×100.</p><p>DNA methylation status in PaWB seedlings with MMS treatment.</p>", "links"=>[], "tags"=>["MSAP Paulownia witches", "Paulownia genomic DNA sequence", "fragment length polymorphism", "phytoplasma", "pattern", "methyl methane sulfonate", "PaWB seedlings", "Paulownia Seedlings Infected Phytoplasmas Reveal", "DNA methylation levels", "pcr", "aflp", "mms"], "article_id"=>1252394, "categories"=>["Biological Sciences"], "users"=>["Xibing Cao", "Guoqiang Fan", "Zhenli Zhao", "Minjie Deng", "Yanpeng Dong"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112533.t004", "stats"=>{"downloads"=>3, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DNA_methylation_status_in_PaWB_seedlings_with_MMS_treatment_/1252394", "title"=>"DNA methylation status in PaWB seedlings with MMS treatment.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-26 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/1807667"], "description"=>"<p>H<sub>1</sub> and M<sub>1</sub>: bands digested by <i>Eco</i>RI/<i>Hpa</i>II (H) and <i>Eco</i>RI/<i>Msp</i>I (M) in PS; H<sub>2</sub> and M<sub>2</sub>: bands digested by H and M in PS-20; H<sub>3</sub> and M<sub>3</sub>: bands digested by H and M in PS-60; H<sub>4</sub> and M<sub>4</sub>: bands digested by H and M in PS-100; H<sub>5</sub> and M<sub>5</sub>: bands digested by H and M in HS; E<sub>25</sub>/HM<sub>52</sub> – E<sub>25</sub>/HM<sub>x</sub>: primer combination; M: Marker; The arrows only indicated part of the methylation patterns between PS and PS-20 (H<sub>1</sub>, M<sub>1</sub>, H<sub>2</sub>, M<sub>2</sub>).</p>", "links"=>[], "tags"=>["MSAP Paulownia witches", "Paulownia genomic DNA sequence", "fragment length polymorphism", "phytoplasma", "pattern", "methyl methane sulfonate", "PaWB seedlings", "Paulownia Seedlings Infected Phytoplasmas Reveal", "DNA methylation levels", "pcr", "aflp", "mms"], "article_id"=>1252376, "categories"=>["Biological Sciences"], "users"=>["Xibing Cao", "Guoqiang Fan", "Zhenli Zhao", "Minjie Deng", "Yanpeng Dong"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112533.g004", "stats"=>{"downloads"=>0, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MSAP_gels_electrophoresis_of_PaWB_seedlings_with_MMS_treatment_/1252376", "title"=>"MSAP gels electrophoresis of PaWB seedlings with MMS treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-26 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/1807678"], "description"=>"<p>HS: Healthy seedlings. The different letters within a column indicate significant difference, while the same letters within a column indicate no significant difference (<i>p</i><0.05).</p><p>Development of MMS treated seedlings.</p>", "links"=>[], "tags"=>["MSAP Paulownia witches", "Paulownia genomic DNA sequence", "fragment length polymorphism", "phytoplasma", "pattern", "methyl methane sulfonate", "PaWB seedlings", "Paulownia Seedlings Infected Phytoplasmas Reveal", "DNA methylation levels", "pcr", "aflp", "mms"], "article_id"=>1252388, "categories"=>["Biological Sciences"], "users"=>["Xibing Cao", "Guoqiang Fan", "Zhenli Zhao", "Minjie Deng", "Yanpeng Dong"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112533.t001", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Development_of_MMS_treated_seedlings_/1252388", "title"=>"Development of MMS treated seedlings.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-26 02:42:41"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
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  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
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Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Plant science", "average_usage"=>[278, 438]}]}
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