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{"title"=>"Dimethyloxalylglycine prevents bone loss in ovariectomized C57BL/6J mice through enhanced angiogenesis and osteogenesis", "type"=>"journal", "authors"=>[{"first_name"=>"Jia", "last_name"=>"Peng", "scopus_author_id"=>"57188965510"}, {"first_name"=>"Zuo Gui", "last_name"=>"Lai", "scopus_author_id"=>"56425136400"}, {"first_name"=>"Zhang Lian", "last_name"=>"Fang", "scopus_author_id"=>"56424645800"}, {"first_name"=>"Shen", "last_name"=>"Xing", "scopus_author_id"=>"56425203500"}, {"first_name"=>"Kang", "last_name"=>"Hui", "scopus_author_id"=>"55909844200"}, {"first_name"=>"Chen", "last_name"=>"Hao", "scopus_author_id"=>"57188960824"}, {"first_name"=>"Qi", "last_name"=>"Jin", "scopus_author_id"=>"35214816800"}, {"first_name"=>"Zhou", "last_name"=>"Qi", "scopus_author_id"=>"56660250900"}, {"first_name"=>"Wang Jin", "last_name"=>"Shen", "scopus_author_id"=>"56424576900"}, {"first_name"=>"Qian Nian", "last_name"=>"Dong", "scopus_author_id"=>"57039494400"}, {"first_name"=>"Zhou Han", "last_name"=>"Bing", "scopus_author_id"=>"57039703500"}, {"first_name"=>"Deng Lian", "last_name"=>"Fu", "scopus_author_id"=>"57039522900"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"600507498", "sgr"=>"84911872705", "pmid"=>"25394221", "scopus"=>"2-s2.0-84911872705", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0112744", "issn"=>"19326203"}, "id"=>"fc4c6ee0-4705-3fd5-a569-ebfa8c053fd8", "abstract"=>"Hypoxia-inducible factor 1-α (HIF-1α) plays a critical role in angiogenesis-osteogenesis coupling during bone development and bone regeneration. Previous studies have shown that 17β-estradiol activates the HIF-1α signaling pathway and that mice with conditional activation of the HIF-1α signaling pathway in osteoblasts are protected from ovariectomy (OVX)-induced bone loss. In addition, it has been shown that hypoxia facilitates the osteogenic differentiation of mesenchymal stem cells (MSCs) and modulates Wnt/β-catenin signaling. Therefore, we hypothesized that activation of the HIF-1α signaling pathway by hypoxia-mimicking agents would prevent bone loss due to estrogen deficiency. In this study, we confirmed the effect of dimethyloxalylglycine (DMOG), a hypoxia-mimicking agent, on the HIF-1α signaling pathway and investigated the effect of DMOG on MSC osteogenic differentiation and the Wnt/β-catenin signaling pathway. We then investigated the effect of DMOG treatment on OVX-induced bone loss. Female C57BL/6J mice were divided into sham, OVX, OVX+L-DMOG (5 mg/kg/day), and OVX+H-DMOG (20 mg/kg/day) groups. At sacrifice, static and dynamic bone histomorphometry were performed with micro computed tomography (micro-CT) and undecalcified sections, respectively. Bone strength was assessed with the three-point bending test, and femur vessels were reconstructed and analyzed by micro-CT. Serum vascular endothelial growth factor (VEGF), osteocalcin, and C-terminal telopeptides of collagen type(CTX) were measured by ELISA. Tartrate-resistant acid phosphatase staining was used to assess osteoclast formation. Alterations in the HIF-1α and Wnt/β-catenin signaling pathways in the bone were detected by western blot. Our results showed that DMOG activated the HIF-1α signaling pathway, which further activated the Wnt/β-catenin signaling pathway and enhanced MSC osteogenic differentiation. The micro-CT results showed that DMOG treatment improved trabecular bone density and restored the bone microarchitecture and blood vessels in OVX mice. Bone strength was also partly restored in DMOG-treated OVX mice. Dynamic bone histomorphometric analysis of the femur metaphysic revealed that DMOG increased the mineralizing surface, mineral apposition rate, and bone formation rate. The serum levels of VEGF and osteocalcin were higher in DMOG-treated OVX mice. However, there were no significant differences in serum CTX or in the number of tartrate-resistant acid phosphatase-stained cells between DMOG-treated OVX mice and OVX mice. Western blot results showed that DMOG administration partly rescued the decrease in HIF-1α and β-catenin expression following ovariectomy. Collectively, these results indicate that DMOG prevents bone loss due to ovariectomy in C57BL/6J mice by enhancing angiogenesis and osteogenesis, which are associated with activated HIF-1α and Wnt/β-catenin signaling pathways.", "link"=>"http://www.mendeley.com/research/dimethyloxalylglycine-prevents-bone-loss-ovariectomized-c57bl6j-mice-through-enhanced-angiogenesis-o", "reader_count"=>20, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>3, "Researcher"=>3, "Student > Ph. D. Student"=>3, "Student > Master"=>5, "Student > Bachelor"=>3, "Lecturer"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>3, "Researcher"=>3, "Student > Ph. D. 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• http://doi.org/10.1111/joa.12506
• http://doi.org/10.1002/jcp.25770
• http://doi.org/10.1096/fj.201802711R
• http://doi.org/10.1002/jcb.25672
• http://doi.org/10.1002/path.4906
• http://doi.org/10.3109/03008207.2016.1171858
• http://doi.org/10.1002/jbm.a.35793
• http://doi.org/10.5582/bst.2018.01046
• http://doi.org/10.1038/cddis.2017.36
• http://doi.org/10.1038/s41467-018-04679-7
• http://doi.org/10.1165/rcmb.2015-0237OC
• http://doi.org/10.1007/s11914-019-00500-6
• http://doi.org/10.1016/j.bone.2015.12.012
• http://doi.org/10.3389/fphys.2016.00416
• http://doi.org/10.1007/s00774-018-0963-7
• http://doi.org/10.1016/j.jep.2018.01.007
• http://doi.org/10.1038/bonekey.2015.35
• http://doi.org/10.1002/adhm.201501018
• http://doi.org/10.1007/s11657-018-0428-6
• http://doi.org/10.1007/s10856-018-6202-x
• http://doi.org/10.1007/s11831-019-09322-2
• http://doi.org/10.1016/j.mce.2015.12.024
• http://doi.org/10.1039/C6TB00065G

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Figshare

• {"files"=>["https://ndownloader.figshare.com/files/1793049"], "description"=>"<p>(A) DMOG abrogated the decrease in BMD and the deterioration in bone microarchitecture induced by OVX, as measured by micro-CT. <b>Morphological analysis of the vasculature within the distal femur from Microfil-perfused mice.</b> (B) OVX decreased the vessel volume at the distal metaphysic of the femur, while DMOG treatment partly restored vessel volumes.</p>", "links"=>[], "tags"=>["mineral apposition rate", "MSC osteogenic differentiation", "trabecular bone density", "vegf", "bone loss", "Ovariectomized C 57BL Mice", "DMOG treatment", "bone formation rate", "Female C 57BL mice", "bone strength", "ctx", "wnt", "Dynamic bone histomorphometric analysis", "elisa", "OVX mice", "hif", "pathway", "Western Blot results", "C 57BL mice"], "article_id"=>1240414, "categories"=>["Biological Sciences"], "users"=>["Jia Peng", "Zuo Gui Lai", "Zhang Lian Fang", "Shen Xing", "Kang Hui", "Chen Hao", "Qi Jin", "Zhou Qi", "Wang Jin Shen", "Qian Nian Dong", "Zhou Han Bing", "Deng Lian Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112744.g003", "stats"=>{"downloads"=>3, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_BMD_and_bone_microarchitecture_of_the_trabecular_bone_in_the_distal_femur_/1240414", "title"=>"BMD and bone microarchitecture of the trabecular bone in the distal femur.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-13 03:48:26"}
• {"files"=>["https://ndownloader.figshare.com/files/1793090"], "description"=>"<div><p>Hypoxia-inducible factor 1-α (HIF-1α) plays a critical role in angiogenesis-osteogenesis coupling during bone development and bone regeneration. Previous studies have shown that 17β-estradiol activates the HIF-1α signaling pathway and that mice with conditional activation of the HIF-1α signaling pathway in osteoblasts are protected from ovariectomy (OVX)-induced bone loss. In addition, it has been shown that hypoxia facilitates the osteogenic differentiation of mesenchymal stem cells (MSCs) and modulates Wnt/β-catenin signaling. Therefore, we hypothesized that activation of the HIF-1α signaling pathway by hypoxia-mimicking agents would prevent bone loss due to estrogen deficiency. In this study, we confirmed the effect of dimethyloxalylglycine (DMOG), a hypoxia-mimicking agent, on the HIF-1α signaling pathway and investigated the effect of DMOG on MSC osteogenic differentiation and the Wnt/β-catenin signaling pathway. We then investigated the effect of DMOG treatment on OVX-induced bone loss. Female C57BL/6J mice were divided into sham, OVX, OVX+L-DMOG (5 mg/kg/day), and OVX+H-DMOG (20 mg/kg/day) groups. At sacrifice, static and dynamic bone histomorphometry were performed with micro computed tomography (micro-CT) and undecalcified sections, respectively. Bone strength was assessed with the three-point bending test, and femur vessels were reconstructed and analyzed by micro-CT. Serum vascular endothelial growth factor (VEGF), osteocalcin, and C-terminal telopeptides of collagen type(CTX) were measured by ELISA. Tartrate-resistant acid phosphatase staining was used to assess osteoclast formation. Alterations in the HIF-1α and Wnt/β-catenin signaling pathways in the bone were detected by western blot. Our results showed that DMOG activated the HIF-1α signaling pathway, which further activated the Wnt/β-catenin signaling pathway and enhanced MSC osteogenic differentiation. The micro-CT results showed that DMOG treatment improved trabecular bone density and restored the bone microarchitecture and blood vessels in OVX mice. Bone strength was also partly restored in DMOG-treated OVX mice. Dynamic bone histomorphometric analysis of the femur metaphysic revealed that DMOG increased the mineralizing surface, mineral apposition rate, and bone formation rate. The serum levels of VEGF and osteocalcin were higher in DMOG-treated OVX mice. However, there were no significant differences in serum CTX or in the number of tartrate-resistant acid phosphatase-stained cells between DMOG-treated OVX mice and OVX mice. Western blot results showed that DMOG administration partly rescued the decrease in HIF-1α and β-catenin expression following ovariectomy. Collectively, these results indicate that DMOG prevents bone loss due to ovariectomy in C57BL/6J mice by enhancing angiogenesis and osteogenesis, which are associated with activated HIF-1α and Wnt/β-catenin signaling pathways.</p></div>", "links"=>[], "tags"=>["mineral apposition rate", "MSC osteogenic differentiation", "trabecular bone density", "vegf", "bone loss", "Ovariectomized C 57BL Mice", "DMOG treatment", "bone formation rate", "Female C 57BL mice", "bone strength", "ctx", "wnt", "Dynamic bone histomorphometric analysis", "elisa", "OVX mice", "hif", "pathway", "Western Blot results", "C 57BL mice"], "article_id"=>1240455, "categories"=>["Biological Sciences"], "users"=>["Jia Peng", "Zuo Gui Lai", "Zhang Lian Fang", "Shen Xing", "Kang Hui", "Chen Hao", "Qi Jin", "Zhou Qi", "Wang Jin Shen", "Qian Nian Dong", "Zhou Han Bing", "Deng Lian Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112744", "stats"=>{"downloads"=>2, "page_views"=>27, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Dimethyloxalylglycine_Prevents_Bone_Loss_in_Ovariectomized_C57BL_6J_Mice_through_Enhanced_Angiogenesis_and_Osteogenesis_/1240455", "title"=>"Dimethyloxalylglycine Prevents Bone Loss in Ovariectomized C57BL/6J Mice through Enhanced Angiogenesis and Osteogenesis", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-13 03:48:26"}
• {"files"=>["https://ndownloader.figshare.com/files/1793034"], "description"=>"<p>(A) 17β-estradiol stabilized HIF-1α under normal oxygen pressure. (B) 17β-estradiol upregulated VEGF protein expression. (C) DMOG enhanced HIF-1α protein expression. (D–E) DMOG upregulated VEGF expression at the mRNA and protein levels. <i>P</i><0.05 for comparisons among the groups designated with an asterisk.</p>", "links"=>[], "tags"=>["mineral apposition rate", "MSC osteogenic differentiation", "trabecular bone density", "vegf", "bone loss", "Ovariectomized C 57BL Mice", "DMOG treatment", "bone formation rate", "Female C 57BL mice", "bone strength", "ctx", "wnt", "Dynamic bone histomorphometric analysis", "elisa", "OVX mice", "hif", "pathway", "Western Blot results", "C 57BL mice"], "article_id"=>1240400, "categories"=>["Biological Sciences"], "users"=>["Jia Peng", "Zuo Gui Lai", "Zhang Lian Fang", "Shen Xing", "Kang Hui", "Chen Hao", "Qi Jin", "Zhou Qi", "Wang Jin Shen", "Qian Nian Dong", "Zhou Han Bing", "Deng Lian Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112744.g001", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_17_946_estradiol_and_DMOG_on_HIF_1_945_signaling_pathway_/1240400", "title"=>"Effect of 17β-estradiol and DMOG on HIF-1α signaling pathway.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-13 03:48:26"}
• {"files"=>["https://ndownloader.figshare.com/files/1793080"], "description"=>"<p>The groups designated with an asterisk shown significant differences with Sham group (<i>P<0.05</i>), the groups designated with a pound sign shown significant differences with OVX group (<i>P<0.05</i>).</p><p>Micro-CT analysis of trabecular BMD, bone microarchitecture and vasculature in the distal femur.</p>", "links"=>[], "tags"=>["mineral apposition rate", "MSC osteogenic differentiation", "trabecular bone density", "vegf", "bone loss", "Ovariectomized C 57BL Mice", "DMOG treatment", "bone formation rate", "Female C 57BL mice", "bone strength", "ctx", "wnt", "Dynamic bone histomorphometric analysis", "elisa", "OVX mice", "hif", "pathway", "Western Blot results", "C 57BL mice"], "article_id"=>1240445, "categories"=>["Biological Sciences"], "users"=>["Jia Peng", "Zuo Gui Lai", "Zhang Lian Fang", "Shen Xing", "Kang Hui", "Chen Hao", "Qi Jin", "Zhou Qi", "Wang Jin Shen", "Qian Nian Dong", "Zhou Han Bing", "Deng Lian Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112744.t002", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Micro_CT_analysis_of_trabecular_BMD_bone_microarchitecture_and_vasculature_in_the_distal_femur_/1240445", "title"=>"Micro-CT analysis of trabecular BMD, bone microarchitecture and vasculature in the distal femur.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-13 03:48:26"}
• {"files"=>["https://ndownloader.figshare.com/files/1793079"], "description"=>"<p>Premiers for Real-time PCR analysis.</p>", "links"=>[], "tags"=>["mineral apposition rate", "MSC osteogenic differentiation", "trabecular bone density", "vegf", "bone loss", "Ovariectomized C 57BL Mice", "DMOG treatment", "bone formation rate", "Female C 57BL mice", "bone strength", "ctx", "wnt", "Dynamic bone histomorphometric analysis", "elisa", "OVX mice", "hif", "pathway", "Western Blot results", "C 57BL mice"], "article_id"=>1240444, "categories"=>["Biological Sciences"], "users"=>["Jia Peng", "Zuo Gui Lai", "Zhang Lian Fang", "Shen Xing", "Kang Hui", "Chen Hao", "Qi Jin", "Zhou Qi", "Wang Jin Shen", "Qian Nian Dong", "Zhou Han Bing", "Deng Lian Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112744.t001", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Premiers_for_Real_time_PCR_analysis_/1240444", "title"=>"Premiers for Real-time PCR analysis.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-13 03:48:26"}
• {"files"=>["https://ndownloader.figshare.com/files/1793076"], "description"=>"<p>(A) HIF-1α, VEGF, and β-catenin expression was lower in OVX mice than in sham mice. However, DMOG treatment increased HIF-1α, VEGF, and β-catenin expression relative to expression in the OVX group. <b>Effects of DMOG administration on tibial ALP, osteocalcin, RUNX-2, and RANKL/OPG mRNA expression in OVX mice assessed by real-time PCR.</b> (B) ALP/β-actin ratio. (C) Osteocalcin/β-actin ratio. (D) RUNX-2/β-actin ratio. (E) RANKL/OPG/β-actin ratio. <i>P</i><0.05 for comparisons among the groups designated with an asterisk.</p>", "links"=>[], "tags"=>["mineral apposition rate", "MSC osteogenic differentiation", "trabecular bone density", "vegf", "bone loss", "Ovariectomized C 57BL Mice", "DMOG treatment", "bone formation rate", "Female C 57BL mice", "bone strength", "ctx", "wnt", "Dynamic bone histomorphometric analysis", "elisa", "OVX mice", "hif", "pathway", "Western Blot results", "C 57BL mice"], "article_id"=>1240441, "categories"=>["Biological Sciences"], "users"=>["Jia Peng", "Zuo Gui Lai", "Zhang Lian Fang", "Shen Xing", "Kang Hui", "Chen Hao", "Qi Jin", "Zhou Qi", "Wang Jin Shen", "Qian Nian Dong", "Zhou Han Bing", "Deng Lian Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112744.g005", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_HIF_1_945_VEGF_and_946_catenin_expression_in_bone_samples_detected_by_western_blot_/1240441", "title"=>"HIF-1α, VEGF, and β-catenin expression in bone samples detected by western blot.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-13 03:48:26"}
• {"files"=>["https://ndownloader.figshare.com/files/1793043"], "description"=>"<p>(A) ALP and alizarin red S staining. (B) DMOG promoted RUNX-2 and osterix mRNA expression. (C) DMOG increased β-catenin mRNA and protein expression. (D) DMOG increased nuclear β-catenin, LEF-1, TCF-1, and HIF-1α protein expression. These effects were attenuated by YC-1. <i>P</i><0.05 for comparisons among the groups designated with an asterisk.</p>", "links"=>[], "tags"=>["mineral apposition rate", "MSC osteogenic differentiation", "trabecular bone density", "vegf", "bone loss", "Ovariectomized C 57BL Mice", "DMOG treatment", "bone formation rate", "Female C 57BL mice", "bone strength", "ctx", "wnt", "Dynamic bone histomorphometric analysis", "elisa", "OVX mice", "hif", "pathway", "Western Blot results", "C 57BL mice"], "article_id"=>1240409, "categories"=>["Biological Sciences"], "users"=>["Jia Peng", "Zuo Gui Lai", "Zhang Lian Fang", "Shen Xing", "Kang Hui", "Chen Hao", "Qi Jin", "Zhou Qi", "Wang Jin Shen", "Qian Nian Dong", "Zhou Han Bing", "Deng Lian Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112744.g002", "stats"=>{"downloads"=>1, "page_views"=>52, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_DMOG_on_MSC_osteogenic_differentiation_and_Wnt_946_catenin_signaling_/1240409", "title"=>"Effect of DMOG on MSC osteogenic differentiation and Wnt/β-catenin signaling.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-13 03:48:26"}
• {"files"=>["https://ndownloader.figshare.com/files/1793073"], "description"=>"<p>(A)Osteoclast counting results showed that DMOG treatment had no obvious effect on OVX-enhanced osteoclast formation. Original magnification, ×100. <b>Dynamic bone formation illustrated by double tetracycline labeling.</b> (B) DMOG administration promoted bone formation, as evidenced by improved MS/BS, MAR, and BFR/BS. Original magnification, ×200. <i>P</i><0.05 for comparisons among the groups designated with an asterisk.</p>", "links"=>[], "tags"=>["mineral apposition rate", "MSC osteogenic differentiation", "trabecular bone density", "vegf", "bone loss", "Ovariectomized C 57BL Mice", "DMOG treatment", "bone formation rate", "Female C 57BL mice", "bone strength", "ctx", "wnt", "Dynamic bone histomorphometric analysis", "elisa", "OVX mice", "hif", "pathway", "Western Blot results", "C 57BL mice"], "article_id"=>1240438, "categories"=>["Biological Sciences"], "users"=>["Jia Peng", "Zuo Gui Lai", "Zhang Lian Fang", "Shen Xing", "Kang Hui", "Chen Hao", "Qi Jin", "Zhou Qi", "Wang Jin Shen", "Qian Nian Dong", "Zhou Han Bing", "Deng Lian Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112744.g004", "stats"=>{"downloads"=>2, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Osteoclast_numbers_assayed_by_tartrate_resistant_acid_phosphatase_staining_of_femoral_sections_/1240438", "title"=>"Osteoclast numbers assayed by tartrate-resistant acid phosphatase staining of femoral sections.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-13 03:48:26"}
• {"files"=>["https://ndownloader.figshare.com/files/1793084"], "description"=>"<p>The groups designated with an asterisk shown significant differences with Sham group (<i>P<0.05</i>), the groups designated with a pound sign shown significant differences with OVX group (<i>P<0.05</i>).</p><p>Biochemical markers of bone in serum as measured by ELISA.</p>", "links"=>[], "tags"=>["mineral apposition rate", "MSC osteogenic differentiation", "trabecular bone density", "vegf", "bone loss", "Ovariectomized C 57BL Mice", "DMOG treatment", "bone formation rate", "Female C 57BL mice", "bone strength", "ctx", "wnt", "Dynamic bone histomorphometric analysis", "elisa", "OVX mice", "hif", "pathway", "Western Blot results", "C 57BL mice"], "article_id"=>1240449, "categories"=>["Biological Sciences"], "users"=>["Jia Peng", "Zuo Gui Lai", "Zhang Lian Fang", "Shen Xing", "Kang Hui", "Chen Hao", "Qi Jin", "Zhou Qi", "Wang Jin Shen", "Qian Nian Dong", "Zhou Han Bing", "Deng Lian Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112744.t004", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Biochemical_markers_of_bone_in_serum_as_measured_by_ELISA_/1240449", "title"=>"Biochemical markers of bone in serum as measured by ELISA.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-13 03:48:26"}
• {"files"=>["https://ndownloader.figshare.com/files/1793082"], "description"=>"<p>The groups designated with an asterisk shown significant differences with Sham group (<i>P<0.05</i>), the groups designated with a pound sign shown significant differences with OVX group (<i>P<0.05</i>).</p><p>Bone quality of the femur as measured by the three-point bending test.</p>", "links"=>[], "tags"=>["mineral apposition rate", "MSC osteogenic differentiation", "trabecular bone density", "vegf", "bone loss", "Ovariectomized C 57BL Mice", "DMOG treatment", "bone formation rate", "Female C 57BL mice", "bone strength", "ctx", "wnt", "Dynamic bone histomorphometric analysis", "elisa", "OVX mice", "hif", "pathway", "Western Blot results", "C 57BL mice"], "article_id"=>1240447, "categories"=>["Biological Sciences"], "users"=>["Jia Peng", "Zuo Gui Lai", "Zhang Lian Fang", "Shen Xing", "Kang Hui", "Chen Hao", "Qi Jin", "Zhou Qi", "Wang Jin Shen", "Qian Nian Dong", "Zhou Han Bing", "Deng Lian Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112744.t003", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Bone_quality_of_the_femur_as_measured_by_the_three_point_bending_test_/1240447", "title"=>"Bone quality of the femur as measured by the three-point bending test.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-11-13 03:48:26"}

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Relative Metric

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F1000Prime