Transportation of Berberine into HepG2, HeLa and SY5Y Cells: A Correlation to Its Anti-Cancer Effect
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{"title"=>"Transportation of berberine into HepG2, hela and SY5Y cells: A correlation to its anti-cancer effect", "type"=>"journal", "authors"=>[{"first_name"=>"Yu Nong", "last_name"=>"Pang", "scopus_author_id"=>"56388196800"}, {"first_name"=>"Yin Wen", "last_name"=>"Liang", "scopus_author_id"=>"55804928400"}, {"first_name"=>"Tian Shi", "last_name"=>"Feng", "scopus_author_id"=>"56373870500"}, {"first_name"=>"Shuang", "last_name"=>"Zhao", "scopus_author_id"=>"55901074700"}, {"first_name"=>"Hao", "last_name"=>"Wu", "scopus_author_id"=>"56424116700"}, {"first_name"=>"Yu Shuang", "last_name"=>"Chai", "scopus_author_id"=>"50261193400"}, {"first_name"=>"Fan", "last_name"=>"Lei", "scopus_author_id"=>"7006258730"}, {"first_name"=>"Yi", "last_name"=>"Ding", "scopus_author_id"=>"56424380200"}, {"first_name"=>"Dong Ming", "last_name"=>"Xing", "scopus_author_id"=>"8238952300"}, {"first_name"=>"Li Jun", "last_name"=>"Du", "scopus_author_id"=>"56563434300"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84911930814", "doi"=>"10.1371/journal.pone.0112937", "pui"=>"600550384", "sgr"=>"84911930814", "issn"=>"19326203"}, "id"=>"a263a9bb-02d7-360c-bb3c-1709b23c7719", "abstract"=>"The anti-cancer activities of berberine (BBR) have been reported extensively in various cancer cell lines. However, the minimal inhibitory concentrations of BBR varied greatly among different cell lines and very few studies have been devoted to elucidate this aspect. In this study, we employed three cancer cell lines, HepG2, HeLa and SY5Y, to compare the transportation and distribution of BBR. HPLC results demonstrated that BBR was capable of penetrating all the cell lines whereas the cumulative concentrations were significantly different. HepG2 cells accumulated higher level of BBR for longer duration than the other two cell lines. Molecular docking studies revealed the BBR binding site on P-glycoprotein 1 (P-gp). In addition, we elucidated that BBR regulated P-gp at both mRNA and protein levels. BBR induced the transcription and translation of P-gp in HeLa and SY5Y cells, whereas BBR inhibited P-gp expression in HepG2 cells. Further study showed that BBR regulates P-gp expression depending on different mechanisms (or affected by different factors) in different cell lines. To summarize, our study has revealed several mechanistic aspects of BBR regulation on P-gp in different cancer cell lines and might shed some useful insights into the use of BBR in the anti-cancer drug development.", "link"=>"http://www.mendeley.com/research/transportation-berberine-hepg2-hela-sy5y-cells-correlation-anticancer-effect", "reader_count"=>5, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Ph. D. Student"=>2, "Student > Master"=>1, "Other"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Ph. D. Student"=>2, "Student > Master"=>1, "Other"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1797045"], "description"=>"<p>(A) Side and (B) top view of the BBR binding pocket of P-gp. (C, D) the overall docking views of BBR in the binding pocket of P-gp. Blue mesh: protein's electron isodensity map around the binding site. Protein are represented as cartoon (transparency = 20%), small molecules and important amino acids (as labeled) are represented as lines (white and green, carbon. red, oxygen. blue, nitrogen). Yellow dash line, hydrogen bond.</p>", "links"=>[], "tags"=>["cancer cell lines", "Molecular docking studies", "BBR binding site", "SY 5Y Cells", "hplc", "HepG 2 cells", "Cell lines"], "article_id"=>1243891, "categories"=>["Uncategorised"], "users"=>["Yu-Nong Pang", "Yin-Wen Liang", "Tian-Shi Feng", "Shuang Zhao", "Hao Wu", "Yu-Shuang Chai", "Fan Lei", "Yi Ding", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112937.g005", "stats"=>{"downloads"=>3, "page_views"=>47, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Molecular_docking_of_berberine_BBR_to_Homo_sapiens_P_gp_/1243891", "title"=>"Molecular docking of berberine (BBR) to Homo sapiens P-gp.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-17 02:49:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1797026"], "description"=>"<p>(A–F) The cytotoxicity and lethality of BBR using MTT assay. (A, B) HepG2, (C, D) HeLa. (E, F) SY5Y. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.005, statistical significance of BBR treated groups relative to control groups. Data are presented as mean ± S.D. from six independent experiments (n = 6).</p>", "links"=>[], "tags"=>["cancer cell lines", "Molecular docking studies", "BBR binding site", "SY 5Y Cells", "hplc", "HepG 2 cells", "Cell lines"], "article_id"=>1243876, "categories"=>["Uncategorised"], "users"=>["Yu-Nong Pang", "Yin-Wen Liang", "Tian-Shi Feng", "Shuang Zhao", "Hao Wu", "Yu-Shuang Chai", "Fan Lei", "Yi Ding", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112937.g001", "stats"=>{"downloads"=>2, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cytotoxicity_of_berberine_BBR_in_three_cell_lines_/1243876", "title"=>"Cytotoxicity of berberine (BBR) in three cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-17 02:49:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1797027"], "description"=>"<p>(A–I) Images of the subcellular location of BBR in cells before and after 0.5 µg/ml BBR administration for 1 h. (A–C) HepG2 cells; (D–F) HeLa cells; (G–I) SY5Y cells. The fluorescence of BBR is shown in green, differential interference contrast (DIC) figures represent the scale of cells and scale bar is 10 µm.</p>", "links"=>[], "tags"=>["cancer cell lines", "Molecular docking studies", "BBR binding site", "SY 5Y Cells", "hplc", "HepG 2 cells", "Cell lines"], "article_id"=>1243877, "categories"=>["Uncategorised"], "users"=>["Yu-Nong Pang", "Yin-Wen Liang", "Tian-Shi Feng", "Shuang Zhao", "Hao Wu", "Yu-Shuang Chai", "Fan Lei", "Yi Ding", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112937.g002", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transportation_of_BBR_through_the_cells_/1243877", "title"=>"Transportation of BBR through the cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-17 02:49:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1797034"], "description"=>"<p>(A–C) HPLC chromatograms. (A) Standard BBR; (B) Blank sample from BBR treated cells; (C) Samples from cells with BBR administration for 12 h. (D) Standard curves of BBR concentration verse AU intensity using HPLC assay. (E–G) Kinetic behavior of BBR in the cells in plot with the administration time in 24 h. (E) HepG2; (F) HeLa; (G) SY5Y cells. (H, I) LC/MS chromatograms of BBR in HepG2 cells after the administration for 24 h. (H) the mass spectrum of BBR; (I) the chromatogram of BBR. Standard BBR was shown in green; the sample was shown in purple color. Data are presented as mean ± S.D. from six independent experiments (n = 6).</p>", "links"=>[], "tags"=>["cancer cell lines", "Molecular docking studies", "BBR binding site", "SY 5Y Cells", "hplc", "HepG 2 cells", "Cell lines"], "article_id"=>1243880, "categories"=>["Uncategorised"], "users"=>["Yu-Nong Pang", "Yin-Wen Liang", "Tian-Shi Feng", "Shuang Zhao", "Hao Wu", "Yu-Shuang Chai", "Fan Lei", "Yi Ding", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112937.g003", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Chromatograms_and_kinetic_behavior_of_BBR_in_the_three_cell_lines_/1243880", "title"=>"Chromatograms and kinetic behavior of BBR in the three cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-17 02:49:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1797046"], "description"=>"<p>(A) mRNA expressions of endogenous P-gp using real-time PCR assay. (B) Western blotting assay showed changes of P-gp protein with BBR treatment. (C) The ratio changes of P-gp versus β-actin. Data are presented as mean ± S.D. from 3 independent experiments (n = 3). The concentration of BBR was 0.5 µg/ml. * <i>v.s.</i> the control, <i>P</i><0.05; ** <i>v.s.</i> the control, <i>P</i><0.005, <i>t</i>-test. For panels in (A) and (C), Left, HepG2; Middle, HeLa; Right, SY5Y.</p>", "links"=>[], "tags"=>["cancer cell lines", "Molecular docking studies", "BBR binding site", "SY 5Y Cells", "hplc", "HepG 2 cells", "Cell lines"], "article_id"=>1243892, "categories"=>["Uncategorised"], "users"=>["Yu-Nong Pang", "Yin-Wen Liang", "Tian-Shi Feng", "Shuang Zhao", "Hao Wu", "Yu-Shuang Chai", "Fan Lei", "Yi Ding", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112937.g006", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_BBR_modulates_the_transcription_and_expression_of_P_gp_/1243892", "title"=>"BBR modulates the transcription and expression of P-gp.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-17 02:49:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1797036"], "description"=>"<p>Cells were treated with 0.5 µg/ml BBR and 0.3 µmol Zosuquidar, the inhibitor of P-gp activity, for 12 h. The intracellular concentrations of BBR were determined by HPLC assay. Data are presented as mean ± S.D from three independent experiments (n = 3). * <i>v.s.</i> the control, <i>P</i><0.05. ** <i>v.s.</i> the control, <i>P</i><0.005, <i>t</i>-test.</p>", "links"=>[], "tags"=>["cancer cell lines", "Molecular docking studies", "BBR binding site", "SY 5Y Cells", "hplc", "HepG 2 cells", "Cell lines"], "article_id"=>1243882, "categories"=>["Uncategorised"], "users"=>["Yu-Nong Pang", "Yin-Wen Liang", "Tian-Shi Feng", "Shuang Zhao", "Hao Wu", "Yu-Shuang Chai", "Fan Lei", "Yi Ding", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112937.g004", "stats"=>{"downloads"=>0, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_P_glycoprotein_P_gp_inhibitor_on_BBR_uptake_in_HepG2_HeLa_and_SY5Y_cells_/1243882", "title"=>"Effect of P-glycoprotein (P-gp) inhibitor on BBR uptake in HepG2, HeLa and SY5Y cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-17 02:49:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/1797047"], "description"=>"<p>(A) Schematic map of P-gp 1 kb promoter fused GFP (P-gp promoter-GFP) construct. Black, P-gp promoter. Green, reading frame of EGFP. (B–D) mRNA expressions of P-gp 1 kb promoter fused GFP in the presence or absence of BBR by real-time PCR assay. (B), HepG2; (C), HeLa; (D), SY5Y. (E) Western blotting assay showed changes of P-gp 1 kb promoter fused GFP protein with BBR treatment. (F–H) The ratio changes of GFP versus β-actin. Data are presented as mean ± S.D. from 3 independent experiments (n = 3). “<i>n.s.”</i> means no statistical significance.</p>", "links"=>[], "tags"=>["cancer cell lines", "Molecular docking studies", "BBR binding site", "SY 5Y Cells", "hplc", "HepG 2 cells", "Cell lines"], "article_id"=>1243893, "categories"=>["Uncategorised"], "users"=>["Yu-Nong Pang", "Yin-Wen Liang", "Tian-Shi Feng", "Shuang Zhao", "Hao Wu", "Yu-Shuang Chai", "Fan Lei", "Yi Ding", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0112937.g007", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_BBR_does_not_act_on_P_gp_promoter_/1243893", "title"=>"BBR does not act on P-gp promoter.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-11-17 02:49:49"}

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