A Fox2-Dependent Fatty Acid ß-Oxidation Pathway Coexists Both in Peroxisomes and Mitochondria of the Ascomycete Yeast Candida lusitaniae
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{"title"=>"A Fox2-dependent fatty acid β-oxidation pathway coexists both in peroxisomes and mitochondria of the ascomycete yeast Candida lusitaniae", "type"=>"journal", "authors"=>[{"first_name"=>"Frédéric", "last_name"=>"Gabriel", "scopus_author_id"=>"57197532658"}, {"first_name"=>"Isabelle", "last_name"=>"Accoceberry", "scopus_author_id"=>"6601979618"}, {"first_name"=>"Jean Jacques", "last_name"=>"Bessoule", "scopus_author_id"=>"6701619765"}, {"first_name"=>"Bénédicte", "last_name"=>"Salin", "scopus_author_id"=>"6602700332"}, {"first_name"=>"Marine", "last_name"=>"Lucas-Guérin", "scopus_author_id"=>"56440221200"}, {"first_name"=>"Stephen", "last_name"=>"Manon", "scopus_author_id"=>"7003928166"}, {"first_name"=>"Karine", "last_name"=>"Dementhon", "scopus_author_id"=>"6506193940"}, {"first_name"=>"Thierry", "last_name"=>"Noël", "scopus_author_id"=>"56276917600"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"600701149", "sgr"=>"84915750757", "issn"=>"19326203", "pmid"=>"25486052", "scopus"=>"2-s2.0-84915750757", "doi"=>"10.1371/journal.pone.0114531"}, "id"=>"877ef55f-0a99-3920-b45c-1528238b20b9", "abstract"=>"It is generally admitted that the ascomycete yeasts of the subphylum Saccharomycotina possess a single fatty acid ß-oxidation pathway located exclusively in peroxisomes, and that they lost mitochondrial ß-oxidation early during evolution. In this work, we showed that mutants of the opportunistic pathogenic yeast Candida lusitaniae which lack the multifunctional enzyme Fox2p, a key enzyme of the ß-oxidation pathway, were still able to grow on fatty acids as the sole carbon source, suggesting that C. lusitaniae harbored an alternative pathway for fatty acid catabolism. By assaying 14Cα-palmitoyl-CoA consumption, we demonstrated that fatty acid catabolism takes place in both peroxisomal and mitochondrial subcellular fractions. We then observed that a fox2Δ null mutant was unable to catabolize fatty acids in the mitochondrial fraction, thus indicating that the mitochondrial pathway was Fox2p-dependent. This finding was confirmed by the immunodetection of Fox2p in protein extracts obtained from purified peroxisomal and mitochondrial fractions. Finally, immunoelectron microscopy provided evidence that Fox2p was localized in both peroxisomes and mitochondria. This work constitutes the first demonstration of the existence of a Fox2p-dependent mitochondrial β-oxidation pathway in an ascomycetous yeast, C. lusitaniae. It also points to the existence of an alternative fatty acid catabolism pathway, probably located in peroxisomes, and functioning in a Fox2p-independent manner.", "link"=>"http://www.mendeley.com/research/fox2dependent-fatty-acid-%CE%B2oxidation-pathway-coexists-both-peroxisomes-mitochondria-ascomycete-yeast", "reader_count"=>16, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Postgraduate"=>2, "Student > Master"=>4}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Postgraduate"=>2, "Student > Master"=>4}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>5, "Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>7}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"France"=>1, "Germany"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1829939"], "description"=>"<p>(A) Thin-layer chromatography (TLC) of lipid extracts obtained from the wild-type, <i>fox2</i>Δ and <i>pxa1</i>Δ strains after growth on YPD or YNB +2% C16:0. The silica gel plates were observed under UV light after spraying of primuline. PL: phospholipids, TAG: tri-acyl-glycerol. (B) Amount of FA contained in PL (light gray) and TAG (dark gray) fractions purified by TLC and quantified by gas-chromatography. C17:0 was used as standard. The sum of the PL and TAG fractions are represented with black bars. Error bars represent SE.</p>", "links"=>[], "tags"=>["2Δ", "Fox 2p", "Ascomycete Yeast Candida lusitaniae", "multifunctional enzyme Fox 2p", "oxidation", "peroxisome", "acid catabolism", "mitochondrial subcellular fractions", "yeast Candida lusitaniae", "Fox 2p manner", "acid catabolism pathway"], "article_id"=>1264481, "categories"=>["Biological Sciences"], "users"=>["Frédéric Gabriel", "Isabelle Accoceberry", "Jean-Jacques Bessoule", "Bénédicte Salin", "Marine Lucas-Guérin", "Stephen Manon", "Karine Dementhon", "Thierry Noël"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114531.g002", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Neutral_lipid_composition_of_the_wild_type_fox2_and_pxa1_strains_/1264481", "title"=>"Neutral lipid composition of the wild-type, <i>fox2</i>Δ and <i>pxa1</i>Δ strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-12-08 03:06:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1829940"], "description"=>"<p>(A) <sup>14</sup>C<sub>α</sub>-palmitoyl-CoA consumption rates after 15 min at 37°C by crude extracts of the mutant and wild-type strains after analysis of the chromatograms using ImageQuant software. Error bars represent SE. (B) Corresponding chromatograms. T0: Amount of <sup>14</sup>C<sub>α</sub>-palmitoyl-CoA initially present in the reaction mix. WT: wild-type strain. P: <sup>14</sup>C<sub>α</sub>-palmitoyl-CoA. E: <sup>14</sup>C<sub>α</sub>-hexadecenoyl-CoA. H: <sup>14</sup>C<sub>α</sub>-3-hydroxyhexadecenoyl-CoA. S: start.</p>", "links"=>[], "tags"=>["2Δ", "Fox 2p", "Ascomycete Yeast Candida lusitaniae", "multifunctional enzyme Fox 2p", "oxidation", "peroxisome", "acid catabolism", "mitochondrial subcellular fractions", "yeast Candida lusitaniae", "Fox 2p manner", "acid catabolism pathway"], "article_id"=>1264482, "categories"=>["Biological Sciences"], "users"=>["Frédéric Gabriel", "Isabelle Accoceberry", "Jean-Jacques Bessoule", "Bénédicte Salin", "Marine Lucas-Guérin", "Stephen Manon", "Karine Dementhon", "Thierry Noël"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114531.g003", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Consumption_of_14_C_945_palmitoyl_CoA_by_cellular_crude_extracts_of_the_C_lusitaniae_icl1_fox2_pxa1_and_wild_type_strains_/1264482", "title"=>"Consumption of <sup>14</sup>C<sub>α</sub>-palmitoyl-CoA by cellular crude extracts of the <i>C. lusitaniae icl1</i>Δ, <i>fox2</i>Δ, <i>pxa1</i>Δ and wild-type strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-12-08 03:06:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1829942"], "description"=>"<p>(A) <sup>14</sup>C<sub>α</sub>-palmitoyl-CoA consumption rates after 15 min at 37°C by peroxisomal and mitochondrial fractions of the mutant and wild-type strains after analysis of the chromatograms using ImageQuant software. The activity observed in the mitochondrial fraction of <i>fox2</i>Δ and wild-type strains were compared using a Student's t-test (*, p = 0.016). Error bars represent SE. (B) Corresponding chromatograms. T0: Amount of <sup>14</sup>C<sub>α</sub>-palmitoyl-CoA initially present in the reaction mix. WT: wild-type strain. P: <sup>14</sup>C<sub>α</sub>-palmitoyl-CoA. E: <sup>14</sup>C<sub>α</sub>-hexadecenoyl-CoA. H: <sup>14</sup>C<sub>α</sub>-3-hydroxyhexadecenoyl-CoA. S: start.</p>", "links"=>[], "tags"=>["2Δ", "Fox 2p", "Ascomycete Yeast Candida lusitaniae", "multifunctional enzyme Fox 2p", "oxidation", "peroxisome", "acid catabolism", "mitochondrial subcellular fractions", "yeast Candida lusitaniae", "Fox 2p manner", "acid catabolism pathway"], "article_id"=>1264484, "categories"=>["Biological Sciences"], "users"=>["Frédéric Gabriel", "Isabelle Accoceberry", "Jean-Jacques Bessoule", "Bénédicte Salin", "Marine Lucas-Guérin", "Stephen Manon", "Karine Dementhon", "Thierry Noël"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114531.g004", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Consumption_of_14_C_945_palmitoyl_CoA_by_peroxisomal_and_mitochondrial_fractions_of_the_C_lusitaniae_fox2_pxa1_and_wild_type_strains_/1264484", "title"=>"Consumption of <sup>14</sup>C<sub>α</sub>-palmitoyl-CoA by peroxisomal and mitochondrial fractions of the <i>C. lusitaniae fox2</i>Δ, <i>pxa1</i>Δ and wild-type strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-12-08 03:06:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1829943"], "description"=>"<p><sup>14</sup>C<sub>α</sub>-palmitoyl-CoA consumption rates after 2 min at 37°C by peroxisomal (diamonds) and mitochondrial (squares) fractions of the wild-type strains. Activity is expressed as nmol of <sup>14</sup>C<sub>α</sub>-palmitoyl-CoA consumed per min after analysis of the chromatograms using ImageQuant software. Error bars represent SE.</p>", "links"=>[], "tags"=>["2Δ", "Fox 2p", "Ascomycete Yeast Candida lusitaniae", "multifunctional enzyme Fox 2p", "oxidation", "peroxisome", "acid catabolism", "mitochondrial subcellular fractions", "yeast Candida lusitaniae", "Fox 2p manner", "acid catabolism pathway"], "article_id"=>1264485, "categories"=>["Biological Sciences"], "users"=>["Frédéric Gabriel", "Isabelle Accoceberry", "Jean-Jacques Bessoule", "Bénédicte Salin", "Marine Lucas-Guérin", "Stephen Manon", "Karine Dementhon", "Thierry Noël"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114531.g005", "stats"=>{"downloads"=>2, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Consumption_of_14_C_945_palmitoyl_CoA_by_different_quantity_of_proteins_from_the_peroxisomal_and_mitochondrial_fractions_of_the_C_lusitaniae_wild_type_strain_/1264485", "title"=>"Consumption of <sup>14</sup>C<sub>α</sub>-palmitoyl-CoA by different quantity of proteins from the peroxisomal and mitochondrial fractions of the <i>C. lusitaniae</i> wild-type strain.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-12-08 03:06:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1829945"], "description"=>"<p>(A) Thirty µg of proteins of the crude extracts and 10 µg of proteins of the peroxisomal and mitochondrial fractions were separated by SDS-PAGE. (B) Signal integration expressed in relative intensity using Quantity One software. Relative amounts of Fox2p are represented with black bars, Icl1p with grey bars, and Cyt<i>c</i> with white bars. Error bars represent SE. Cyt<i>c</i>: cytochrome <i>c</i>, WT: wild type, CE: crude extract, P: peroxisomal fraction, M: mitochondrial fraction.</p>", "links"=>[], "tags"=>["2Δ", "Fox 2p", "Ascomycete Yeast Candida lusitaniae", "multifunctional enzyme Fox 2p", "oxidation", "peroxisome", "acid catabolism", "mitochondrial subcellular fractions", "yeast Candida lusitaniae", "Fox 2p manner", "acid catabolism pathway"], "article_id"=>1264487, "categories"=>["Biological Sciences"], "users"=>["Frédéric Gabriel", "Isabelle Accoceberry", "Jean-Jacques Bessoule", "Bénédicte Salin", "Marine Lucas-Guérin", "Stephen Manon", "Karine Dementhon", "Thierry Noël"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114531.g006", "stats"=>{"downloads"=>0, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunodetection_of_Fox2p_Icl1p_and_cytochrome_c_by_Western_blot_in_the_C_lusitaniae_fox2_and_wild_type_strains_/1264487", "title"=>"Immunodetection of Fox2p, Icl1p and cytochrome c by Western-blot in the <i>C. lusitaniae fox2</i>Δ and wild-type strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-12-08 03:06:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1829947"], "description"=>"<p>Glucose-grown cells (A and B) or oleate-induced cells (C to F) of wild-type (A, C, and E) or <i>fox2</i>Δ (B, D, and F) strains were fixed and prepared for electron microscopy (A to D) or immunoelectron microscopy (E and F). In E and F, cryosections were incubated with a polyclonal hen primary antibody directed against <i>C. lusitaniae</i> Fox2p and antibodies were revealed with immunogold particles conjugated to anti-hen antibodies. P, peroxisome, M, mitochondrion, N, nucleus. Bars, 200 nm.</p>", "links"=>[], "tags"=>["2Δ", "Fox 2p", "Ascomycete Yeast Candida lusitaniae", "multifunctional enzyme Fox 2p", "oxidation", "peroxisome", "acid catabolism", "mitochondrial subcellular fractions", "yeast Candida lusitaniae", "Fox 2p manner", "acid catabolism pathway"], "article_id"=>1264489, "categories"=>["Biological Sciences"], "users"=>["Frédéric Gabriel", "Isabelle Accoceberry", "Jean-Jacques Bessoule", "Bénédicte Salin", "Marine Lucas-Guérin", "Stephen Manon", "Karine Dementhon", "Thierry Noël"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114531.g007", "stats"=>{"downloads"=>3, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characterization_of_the_wild_type_and_fox2_strains_by_electron_and_immunoelectron_microscopy_/1264489", "title"=>"Characterization of the wild-type and <i>fox2</i>Δ strains by electron and immunoelectron microscopy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-12-08 03:06:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1829949"], "description"=>"<p>Cells were pregrown to mid-log phase in YPD, washed, resuspended in water to an OD<sub>600</sub> of 0.5 (about 8.5×10<sup>6</sup> cells/ml), and serially diluted fivefold. Drops of 7 µl of each dilution were deposited onto solid minimum media (YNB), containing 2% of either glucose, acetate, glycerol, or fatty acid as the sole carbon source. Growth was observed after 3 days (glucose) or 5 days (acetate, glycerol, fatty acids) of incubation at 30°C. Growth on the first dilution (1∶5) was noted ‘+’, on the second dilution (1∶5<sup>2</sup>) was noted ‘++’, on the third dilution (1∶5<sup>3</sup>) was noted ‘+++’, on the fourth dilution (1∶5<sup>4</sup>) was noted ‘++++’, on the fifth dilution (1∶5<sup>5</sup>) was noted ‘+++++’, and no growth was noted ‘−’.</p><p>Growth characteristics of mutant, reintegrant, and wild-type strains on different carbon sources at 30°C.</p>", "links"=>[], "tags"=>["2Δ", "Fox 2p", "Ascomycete Yeast Candida lusitaniae", "multifunctional enzyme Fox 2p", "oxidation", "peroxisome", "acid catabolism", "mitochondrial subcellular fractions", "yeast Candida lusitaniae", "Fox 2p manner", "acid catabolism pathway"], "article_id"=>1264491, "categories"=>["Biological Sciences"], "users"=>["Frédéric Gabriel", "Isabelle Accoceberry", "Jean-Jacques Bessoule", "Bénédicte Salin", "Marine Lucas-Guérin", "Stephen Manon", "Karine Dementhon", "Thierry Noël"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114531.t001", "stats"=>{"downloads"=>5, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Growth_characteristics_of_mutant_reintegrant_and_wild_type_strains_on_different_carbon_sources_at_30_176_C_/1264491", "title"=>"Growth characteristics of mutant, reintegrant, and wild-type strains on different carbon sources at 30°C.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-12-08 03:06:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1829950"], "description"=>"<p>Sp activity: specific activity</p>a<p>10 µg of proteins</p>b<p>Catalase specific activity was divided by cytochrome c oxidase specific activity</p>c<p>Catalase specific activity in mitochondrial fraction was divided by catalase specific activity in peroxisomal fraction</p><p>Purification of peroxisomes and mitochondria from cells grown in induction medium.</p>", "links"=>[], "tags"=>["2Δ", "Fox 2p", "Ascomycete Yeast Candida lusitaniae", "multifunctional enzyme Fox 2p", "oxidation", "peroxisome", "acid catabolism", "mitochondrial subcellular fractions", "yeast Candida lusitaniae", "Fox 2p manner", "acid catabolism pathway"], "article_id"=>1264492, "categories"=>["Biological Sciences"], "users"=>["Frédéric Gabriel", "Isabelle Accoceberry", "Jean-Jacques Bessoule", "Bénédicte Salin", "Marine Lucas-Guérin", "Stephen Manon", "Karine Dementhon", "Thierry Noël"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114531.t002", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Purification_of_peroxisomes_and_mitochondria_from_cells_grown_in_induction_medium_/1264492", "title"=>"Purification of peroxisomes and mitochondria from cells grown in induction medium.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-12-08 03:06:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1829951"], "description"=>"a<p>Only cell sections containing observable nucleus and vacuole were considered.</p><p>Number and size of peroxisomes and number of mitochondria sections observed in <i>Candida lusitaniae</i> cells by electron microscopy.</p>", "links"=>[], "tags"=>["2Δ", "Fox 2p", "Ascomycete Yeast Candida lusitaniae", "multifunctional enzyme Fox 2p", "oxidation", "peroxisome", "acid catabolism", "mitochondrial subcellular fractions", "yeast Candida lusitaniae", "Fox 2p manner", "acid catabolism pathway"], "article_id"=>1264493, "categories"=>["Biological Sciences"], "users"=>["Frédéric Gabriel", "Isabelle Accoceberry", "Jean-Jacques Bessoule", "Bénédicte Salin", "Marine Lucas-Guérin", "Stephen Manon", "Karine Dementhon", "Thierry Noël"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114531.t003", "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Number_and_size_of_peroxisomes_and_number_of_mitochondria_sections_observed_in_Candida_lusitaniae_cells_by_electron_microscopy_/1264493", "title"=>"Number and size of peroxisomes and number of mitochondria sections observed in <i>Candida lusitaniae</i> cells by electron microscopy.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-12-08 03:06:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1829952", "https://ndownloader.figshare.com/files/1829953", "https://ndownloader.figshare.com/files/1829954", "https://ndownloader.figshare.com/files/1829955", "https://ndownloader.figshare.com/files/1829956", "https://ndownloader.figshare.com/files/1829957"], "description"=>"<div><p>It is generally admitted that the ascomycete yeasts of the subphylum <i>Saccharomycotina</i> possess a single fatty acid ß-oxidation pathway located exclusively in peroxisomes, and that they lost mitochondrial ß-oxidation early during evolution. In this work, we showed that mutants of the opportunistic pathogenic yeast <i>Candida lusitaniae</i> which lack the multifunctional enzyme Fox2p, a key enzyme of the ß-oxidation pathway, were still able to grow on fatty acids as the sole carbon source, suggesting that <i>C. lusitaniae</i> harbored an alternative pathway for fatty acid catabolism. By assaying <sup>14</sup>C<sub>α</sub>-palmitoyl-CoA consumption, we demonstrated that fatty acid catabolism takes place in both peroxisomal and mitochondrial subcellular fractions. We then observed that a <i>fox2</i>Δ null mutant was unable to catabolize fatty acids in the mitochondrial fraction, thus indicating that the mitochondrial pathway was Fox2p-dependent. This finding was confirmed by the immunodetection of Fox2p in protein extracts obtained from purified peroxisomal and mitochondrial fractions. Finally, immunoelectron microscopy provided evidence that Fox2p was localized in both peroxisomes and mitochondria. This work constitutes the first demonstration of the existence of a Fox2p-dependent mitochondrial β-oxidation pathway in an ascomycetous yeast, <i>C. lusitaniae</i>. It also points to the existence of an alternative fatty acid catabolism pathway, probably located in peroxisomes, and functioning in a Fox2p-independent manner.</p></div>", "links"=>[], "tags"=>["2Δ", "Fox 2p", "Ascomycete Yeast Candida lusitaniae", "multifunctional enzyme Fox 2p", "oxidation", "peroxisome", "acid catabolism", "mitochondrial subcellular fractions", "yeast Candida lusitaniae", "Fox 2p manner", "acid catabolism pathway"], "article_id"=>1264494, "categories"=>["Biological Sciences"], "users"=>["Frédéric Gabriel", "Isabelle Accoceberry", "Jean-Jacques Bessoule", "Bénédicte Salin", "Marine Lucas-Guérin", "Stephen Manon", "Karine Dementhon", "Thierry Noël"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0114531.s001", "https://dx.doi.org/10.1371/journal.pone.0114531.s002", "https://dx.doi.org/10.1371/journal.pone.0114531.s003", "https://dx.doi.org/10.1371/journal.pone.0114531.s004", "https://dx.doi.org/10.1371/journal.pone.0114531.s005", "https://dx.doi.org/10.1371/journal.pone.0114531.s006"], "stats"=>{"downloads"=>14, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/A_Fox2_Dependent_Fatty_Acid_Oxidation_Pathway_Coexists_Both_in_Peroxisomes_and_Mitochondria_of_the_Ascomycete_Yeast_Candida_lusitaniae_/1264494", "title"=>"A Fox2-Dependent Fatty Acid ß-Oxidation Pathway Coexists Both in Peroxisomes and Mitochondria of the Ascomycete Yeast <i>Candida lusitaniae</i>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-12-08 03:06:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1829935"], "description"=>"<p>Model adapted from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114531#pone.0114531-Strijbis1\" target=\"_blank\">[3]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114531#pone.0114531-Hiltunen1\" target=\"_blank\">[4]</a>. The β-oxidation pathway is exclusively peroxisomal. The localization of the specific enzymes of the glyoxylate cycle (Icl1p and Mls1p) is susceptible to variations according to the species (i. e. Icl1p is peroxisomal in <i>C. albicans</i> and cytoplasmic in <i>S. cerevisiae</i>). Mls1p: Malate synthase. CAT: carnitine acetyl-transferase.</p>", "links"=>[], "tags"=>["2Δ", "Fox 2p", "Ascomycete Yeast Candida lusitaniae", "multifunctional enzyme Fox 2p", "oxidation", "peroxisome", "acid catabolism", "mitochondrial subcellular fractions", "yeast Candida lusitaniae", "Fox 2p manner", "acid catabolism pathway"], "article_id"=>1264477, "categories"=>["Biological Sciences"], "users"=>["Frédéric Gabriel", "Isabelle Accoceberry", "Jean-Jacques Bessoule", "Bénédicte Salin", "Marine Lucas-Guérin", "Stephen Manon", "Karine Dementhon", "Thierry Noël"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114531.g001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Fatty_acid_catabolism_in_ascomycetous_yeasts_/1264477", "title"=>"Fatty acid catabolism in ascomycetous yeasts.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-12-08 03:06:31"}

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Relative Metric

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