Group 1 Allergen Genes in Two Species of House Dust Mites, Dermatophagoides farinae and D. pteronyssinus (Acari: Pyroglyphidae): Direct Sequencing, Characterization and Polymorphism
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{"title"=>"Group 1 allergen genes in two species of house dust mites, dermatophagoides farinae and D. pteronyssinus (Acari: Pyroglyphidae): Direct sequencing, characterization and polymorphism", "type"=>"journal", "authors"=>[{"first_name"=>"Rubaba Hamid", "last_name"=>"Shafique", "scopus_author_id"=>"56444392100"}, {"first_name"=>"Pavel B.", "last_name"=>"Klimov", "scopus_author_id"=>"7007072278"}, {"first_name"=>"Muhammad", "last_name"=>"Inam", "scopus_author_id"=>"57193142522"}, {"first_name"=>"Farhana Riaz", "last_name"=>"Chaudhary", "scopus_author_id"=>"56444203500"}, {"first_name"=>"Barry M.", "last_name"=>"OConnor", "scopus_author_id"=>"6701619879"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84916920050", "sgr"=>"84916920050", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0114636", "pmid"=>"25494056", "pui"=>"600717231"}, "id"=>"a956c81e-5986-3503-a0cb-4206201fa8bc", "abstract"=>"Group 1 allergens of Dermatophagoides farinae (Der f 1) and D. pteronyssinus (Der p 1) dominate overall allergic responses in house dust mite allergy patients. The need for accurate identification and characterization of representative variants of group 1 allergens in any given geographic locality has been emphasized for development of appropriate allergen extracts. Regional amino acid sequence polymorphism has been described but the extent of this polymorphism is not well understood. Such data are completely absent for the USA and many other countries. Most previous studies used cDNA libraries generated by reverse transcriptase (RT-PCR) and/or primers amplifying shorter fragments of this gene. Using novel species-specific primers and direct PCR, we document group 1 allergen gene sequence polymorphism in populations of D. farinae and D. pteronyssinus from the USA and Pakistan. We report two novel introns (nt pos 87 and 291) in both species, and the absence of intron 3 in Der p 1. Thirteen silent and one novel non-synonymous mutation (Tryptophan W197 to Arginine R197) were detected in D. farinae. The potential medical significance of the latter mutation is discussed. Two haplotypes of the Der f 1 gene were identified, haplotype 1 (63%) was more frequent than haplotype 2 (18%). Polymorphism in Der f 1 displayed geographical localization, since both haplotypes were present in mite populations from Pakistan whereas haplotype 1 was observed only in the USA. In Der p 1, a silent mutation at nt (aa) position 1011(149) and four non-synonymous mutations at positions 589(50), 935(124), 971(136), 1268(215) were observed. These mutations were reported from many other geographic regions, suggesting that polymorphism in the Der p 1 gene is panmictic. The extent of polymorphism in both genes is substantially lower than that reported previously (0.10-0.16% vs 0.31-0.49%), indicating the need for careful evaluation of potential polymerase errors in studies utilizing RT-PCR.", "link"=>"http://www.mendeley.com/research/group-1-allergen-genes-two-species-house-dust-mites-dermatophagoides-farinae-d-pteronyssinus-acari-p", "reader_count"=>18, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Librarian"=>1, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Librarian"=>1, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Materials Science"=>1, "Agricultural and Biological Sciences"=>12, "Medicine and Dentistry"=>2, "Arts and Humanities"=>1}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>12}, "Unspecified"=>{"Unspecified"=>2}, "Arts and Humanities"=>{"Arts and Humanities"=>1}}, "group_count"=>2}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1832767"], "description"=>"<p>Active site regions are shown in yellow, amino acid residues making 4C1 binding epitope (pink) and calcium binding epitope (green); “*”: identical, “:”: conserved and “.”: semi-conserved sites.</p>", "links"=>[], "tags"=>["house dust mite allergy patients", "nt pos 87", "acid sequence polymorphism", "pcr", "Der f 1", "pteronyssinu", "Der p 1.", "mutation", "Der p 1 gene", "group 1 Allergen Genes", "haplotype", "Der f 1 gene", "document group 1 allergen gene sequence polymorphism", "group 1 allergens", "Tryptophan W 197", "usa", "Polymorphism Group 1 allergens", "House Dust Mites", "Dermatophagoides farinae"], "article_id"=>1266882, "categories"=>["Uncategorised"], "users"=>["Rubaba Hamid Shafique", "Pavel B. Klimov", "Muhammad Inam", "Farhana Riaz Chaudhary", "Barry M. OConnor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114636.g002", "stats"=>{"downloads"=>0, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alignment_of_group_1_allergens_Cysteine_proteases_of_selected_mite_species_/1266882", "title"=>"Alignment of group 1 allergens (Cysteine proteases) of selected mite species.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-12-10 02:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1832770"], "description"=>"<p>A: specimen RS33; B: specimen RS31.</p>", "links"=>[], "tags"=>["house dust mite allergy patients", "nt pos 87", "acid sequence polymorphism", "pcr", "Der f 1", "pteronyssinu", "Der p 1.", "mutation", "Der p 1 gene", "group 1 Allergen Genes", "haplotype", "Der f 1 gene", "document group 1 allergen gene sequence polymorphism", "group 1 allergens", "Tryptophan W 197", "usa", "Polymorphism Group 1 allergens", "House Dust Mites", "Dermatophagoides farinae"], "article_id"=>1266886, "categories"=>["Uncategorised"], "users"=>["Rubaba Hamid Shafique", "Pavel B. Klimov", "Muhammad Inam", "Farhana Riaz Chaudhary", "Barry M. OConnor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114636.g003", "stats"=>{"downloads"=>9, "page_views"=>121, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_I_TASSER_result_showing_tertiary_structure_predictions_for_RS33_Tryptophan_variant_and_RS31_Arginine_variant_of_Der_f_1_protein_36_38_/1266886", "title"=>"I-TASSER result showing tertiary structure predictions for RS33 (Tryptophan variant) and RS31 (Arginine variant) of Der f 1 protein [36]–[38].", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-12-10 02:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1832772"], "description"=>"<p>For each node, nonparametric bootstrap support values (100 replicates) are indicated. The tree was inferred in RAxML ver.7.5.5 <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114636#pone.0114636-Stamatakis1\" target=\"_blank\">[39]</a> using the WAGF model of amino acid evolution as found in PartitionFinder ver. 1.1.1 <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114636#pone.0114636-Lanfear1\" target=\"_blank\">[40]</a>.</p>", "links"=>[], "tags"=>["house dust mite allergy patients", "nt pos 87", "acid sequence polymorphism", "pcr", "Der f 1", "pteronyssinu", "Der p 1.", "mutation", "Der p 1 gene", "group 1 Allergen Genes", "haplotype", "Der f 1 gene", "document group 1 allergen gene sequence polymorphism", "group 1 allergens", "Tryptophan W 197", "usa", "Polymorphism Group 1 allergens", "House Dust Mites", "Dermatophagoides farinae"], "article_id"=>1266888, "categories"=>["Uncategorised"], "users"=>["Rubaba Hamid Shafique", "Pavel B. Klimov", "Muhammad Inam", "Farhana Riaz Chaudhary", "Barry M. OConnor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114636.g004", "stats"=>{"downloads"=>0, "page_views"=>37, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Maximum_likelihood_tree_of_the_group_1_allergen_protein_of_acariform_mites_/1266888", "title"=>"Maximum likelihood tree of the group 1 allergen protein of acariform mites.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-12-10 02:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1832775"], "description"=>"<p>In primer names: DF - <i>Dermatophagoides farinae</i>, DP - <i>D. pteronyssinus</i>; P – outer primer; P.Ch. – inner primer; F – forward; R – reverse; T3– has T3 tail (underlined in primer sequence); SP6– has SP6 tail (underlined).</p><p>Species-specific oligonucleotide primers used in nested amplification of the group 1 allergen gene (Der f 1 and Der p 1).</p>", "links"=>[], "tags"=>["house dust mite allergy patients", "nt pos 87", "acid sequence polymorphism", "pcr", "Der f 1", "pteronyssinu", "Der p 1.", "mutation", "Der p 1 gene", "group 1 Allergen Genes", "haplotype", "Der f 1 gene", "document group 1 allergen gene sequence polymorphism", "group 1 allergens", "Tryptophan W 197", "usa", "Polymorphism Group 1 allergens", "House Dust Mites", "Dermatophagoides farinae"], "article_id"=>1266891, "categories"=>["Uncategorised"], "users"=>["Rubaba Hamid Shafique", "Pavel B. Klimov", "Muhammad Inam", "Farhana Riaz Chaudhary", "Barry M. OConnor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114636.t001", "stats"=>{"downloads"=>4, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Species_specific_oligonucleotide_primers_used_in_nested_amplification_of_the_group_1_allergen_gene_Der_f_1_and_Der_p_1_/1266891", "title"=>"Species-specific oligonucleotide primers used in nested amplification of the group 1 allergen gene (Der f 1 and Der p 1).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-12-10 02:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1832776"], "description"=>"<p>PK: Pakistan; USA: United States of America;</p>1<p>not reported in previous studies;</p>2<p>Novel silent (synonymous) mutation;</p>3<p>Novel non silent (non-synonymous) mutation. GenBank accession numbers for the sequences resulted from our study are given in parentheses.</p><p>Exons, introns, and sequence polymorphism in the group 1 allergen-encoding gene of two species of house dust mites from Pakistan and the USA.</p>", "links"=>[], "tags"=>["house dust mite allergy patients", "nt pos 87", "acid sequence polymorphism", "pcr", "Der f 1", "pteronyssinu", "Der p 1.", "mutation", "Der p 1 gene", "group 1 Allergen Genes", "haplotype", "Der f 1 gene", "document group 1 allergen gene sequence polymorphism", "group 1 allergens", "Tryptophan W 197", "usa", "Polymorphism Group 1 allergens", "House Dust Mites", "Dermatophagoides farinae"], "article_id"=>1266892, "categories"=>["Uncategorised"], "users"=>["Rubaba Hamid Shafique", "Pavel B. Klimov", "Muhammad Inam", "Farhana Riaz Chaudhary", "Barry M. OConnor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114636.t002", "stats"=>{"downloads"=>3, "page_views"=>34, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Exons_introns_and_sequence_polymorphism_in_the_group_1_allergen_encoding_gene_of_two_species_of_house_dust_mites_from_Pakistan_and_the_USA_/1266892", "title"=>"Exons, introns, and sequence polymorphism in the group 1 allergen-encoding gene of two species of house dust mites from Pakistan and the USA.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-12-10 02:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1832778"], "description"=>"<p>Superscript on amino acid symbol indicates the position in polypeptide (N.R.: not reported; “a.a/a.a”: higher frequency/lower frequency reported; “-”: no mutation</p><p>Geographical polymorphism in the Der p 1 allergen.</p>", "links"=>[], "tags"=>["house dust mite allergy patients", "nt pos 87", "acid sequence polymorphism", "pcr", "Der f 1", "pteronyssinu", "Der p 1.", "mutation", "Der p 1 gene", "group 1 Allergen Genes", "haplotype", "Der f 1 gene", "document group 1 allergen gene sequence polymorphism", "group 1 allergens", "Tryptophan W 197", "usa", "Polymorphism Group 1 allergens", "House Dust Mites", "Dermatophagoides farinae"], "article_id"=>1266894, "categories"=>["Uncategorised"], "users"=>["Rubaba Hamid Shafique", "Pavel B. Klimov", "Muhammad Inam", "Farhana Riaz Chaudhary", "Barry M. OConnor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114636.t003", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Geographical_polymorphism_in_the_Der_p_1_allergen_/1266894", "title"=>"Geographical polymorphism in the Der p 1 allergen.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-12-10 02:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1832782"], "description"=>"<p>Der f 1: D. farinae; Der p 1: D. pteronyssinus; Eur m 1: E. maynei; Pso o 1: P. ovis; Sar s 1: S. scabiei; Aca s 1: A. siro; Blo t 1: B. tropicalis; Tyr p 1: T. putrescentiae.</p><p>Percent identity matrix of aligned group1 allergens.</p>", "links"=>[], "tags"=>["house dust mite allergy patients", "nt pos 87", "acid sequence polymorphism", "pcr", "Der f 1", "pteronyssinu", "Der p 1.", "mutation", "Der p 1 gene", "group 1 Allergen Genes", "haplotype", "Der f 1 gene", "document group 1 allergen gene sequence polymorphism", "group 1 allergens", "Tryptophan W 197", "usa", "Polymorphism Group 1 allergens", "House Dust Mites", "Dermatophagoides farinae"], "article_id"=>1266896, "categories"=>["Uncategorised"], "users"=>["Rubaba Hamid Shafique", "Pavel B. Klimov", "Muhammad Inam", "Farhana Riaz Chaudhary", "Barry M. OConnor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114636.t004", "stats"=>{"downloads"=>8, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Percent_identity_matrix_of_aligned_group1_allergens_/1266896", "title"=>"Percent identity matrix of aligned group1 allergens.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-12-10 02:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1832786"], "description"=>"<p>A high percent identity score (>50) was observed in Der p 1, Eur m 1, Pso o 1 compared to Der f 1, indicating possibility of cross-reactivity among these allergens.</p><p>Comparison of group 1 allergen conserved amino acid residues involved in 4C1mAb and Ca<sup>+</sup> binding epitopes in eight mite species.</p>", "links"=>[], "tags"=>["house dust mite allergy patients", "nt pos 87", "acid sequence polymorphism", "pcr", "Der f 1", "pteronyssinu", "Der p 1.", "mutation", "Der p 1 gene", "group 1 Allergen Genes", "haplotype", "Der f 1 gene", "document group 1 allergen gene sequence polymorphism", "group 1 allergens", "Tryptophan W 197", "usa", "Polymorphism Group 1 allergens", "House Dust Mites", "Dermatophagoides farinae"], "article_id"=>1266899, "categories"=>["Uncategorised"], "users"=>["Rubaba Hamid Shafique", "Pavel B. Klimov", "Muhammad Inam", "Farhana Riaz Chaudhary", "Barry M. OConnor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114636.t005", "stats"=>{"downloads"=>4, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_group_1_allergen_conserved_amino_acid_residues_involved_in_4C1mAb_and_Ca_binding_epitopes_in_eight_mite_species_/1266899", "title"=>"Comparison of group 1 allergen conserved amino acid residues involved in 4C1mAb and Ca<sup>+</sup> binding epitopes in eight mite species.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-12-10 02:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1832748"], "description"=>"<p>UTR: untranslated regions; (numbers): nucleotide position for introns. The blank region with dashed outlines at exon 1 indicates partial 5′end of genes (given our sequences). T3/SP4 tails (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114636#pone-0114636-t001\" target=\"_blank\">table 1</a>) in primer sequences are not shown.</p>", "links"=>[], "tags"=>["house dust mite allergy patients", "nt pos 87", "acid sequence polymorphism", "pcr", "Der f 1", "pteronyssinu", "Der p 1.", "mutation", "Der p 1 gene", "group 1 Allergen Genes", "haplotype", "Der f 1 gene", "document group 1 allergen gene sequence polymorphism", "group 1 allergens", "Tryptophan W 197", "usa", "Polymorphism Group 1 allergens", "House Dust Mites", "Dermatophagoides farinae"], "article_id"=>1266872, "categories"=>["Uncategorised"], "users"=>["Rubaba Hamid Shafique", "Pavel B. Klimov", "Muhammad Inam", "Farhana Riaz Chaudhary", "Barry M. OConnor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0114636.g001", "stats"=>{"downloads"=>1, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gene_map_of_Der_f_1_A_and_Der_p_1_B_allergens_amplified_by_nested_PCR_/1266872", "title"=>"Gene map of Der f 1 (A) and Der p 1 (B) allergens amplified by nested PCR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-12-10 02:58:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1832789", "https://ndownloader.figshare.com/files/1832790"], "description"=>"<div><p>Group 1 allergens of <i>Dermatophagoides farinae</i> (Der f 1) and <i>D. pteronyssinus</i> (Der p 1) dominate overall allergic responses in house dust mite allergy patients. The need for accurate identification and characterization of representative variants of group 1 allergens in any given geographic locality has been emphasized for development of appropriate allergen extracts. Regional amino acid sequence polymorphism has been described but the extent of this polymorphism is not well understood. Such data are completely absent for the USA and many other countries. Most previous studies used cDNA libraries generated by reverse transcriptase (RT-PCR) and/or primers amplifying shorter fragments of this gene. Using novel species-specific primers and direct PCR, we document group 1 allergen gene sequence polymorphism in populations of <i>D. farinae</i> and <i>D. pteronyssinus</i> from the USA and Pakistan. We report two novel introns (nt pos 87 and 291) in both species, and the absence of intron 3 in Der p 1. Thirteen silent and one novel non-synonymous mutation (Tryptophan W<sup>197</sup> to Arginine R<sup>197</sup>) were detected in <i>D. farinae</i>. The potential medical significance of the latter mutation is discussed. Two haplotypes of the Der f 1 gene were identified, haplotype 1 (63%) was more frequent than haplotype 2 (18%). Polymorphism in Der f 1 displayed geographical localization, since both haplotypes were present in mite populations from Pakistan whereas haplotype 1 was observed only in the USA. In Der p 1, a silent mutation at nt (aa) position 1011(149) and four non-synonymous mutations at positions 589(50), 935(124), 971(136), 1268(215) were observed. These mutations were reported from many other geographic regions, suggesting that polymorphism in the Der p 1 gene is panmictic. The extent of polymorphism in both genes is substantially lower than that reported previously (0.10–0.16% vs 0.31–0.49%), indicating the need for careful evaluation of potential polymerase errors in studies utilizing RT-PCR.</p></div>", "links"=>[], "tags"=>["house dust mite allergy patients", "nt pos 87", "acid sequence polymorphism", "pcr", "Der f 1", "pteronyssinu", "Der p 1.", "mutation", "Der p 1 gene", "group 1 Allergen Genes", "haplotype", "Der f 1 gene", "document group 1 allergen gene sequence polymorphism", "group 1 allergens", "Tryptophan W 197", "usa", "Polymorphism Group 1 allergens", "House Dust Mites", "Dermatophagoides farinae"], "article_id"=>1266905, "categories"=>["Uncategorised"], "users"=>["Rubaba Hamid Shafique", "Pavel B. Klimov", "Muhammad Inam", "Farhana Riaz Chaudhary", "Barry M. OConnor"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0114636.s001", "https://dx.doi.org/10.1371/journal.pone.0114636.s002"], "stats"=>{"downloads"=>1, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Group_1_Allergen_Genes_in_Two_Species_of_House_Dust_Mites_Dermatophagoides_farinae_and_D_pteronyssinus_Acari_Pyroglyphidae_Direct_Sequencing_Characterization_and_Polymorphism/1266905", "title"=>"Group 1 Allergen Genes in Two Species of House Dust Mites, <i>Dermatophagoides farinae</i> and <i>D. pteronyssinus</i> (Acari: Pyroglyphidae): Direct Sequencing, Characterization and Polymorphism", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-12-10 02:58:35"}

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  • {"unique-ip"=>"17", "full-text"=>"21", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"34", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"20", "full-text"=>"26", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"20", "full-text"=>"22", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"25", "full-text"=>"25", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"33", "full-text"=>"32", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"26", "full-text"=>"26", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"22", "full-text"=>"27", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[]}
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