Characterization and Biological Activity of Taishan Pinus massoniana Pollen Polysaccharide In Vitro
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{"title"=>"Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro", "type"=>"journal", "authors"=>[{"first_name"=>"Shifa", "last_name"=>"Yang", "scopus_author_id"=>"55368492700"}, {"first_name"=>"Kai", "last_name"=>"Wei", "scopus_author_id"=>"37053039500"}, {"first_name"=>"Fengjuan", "last_name"=>"Jia", "scopus_author_id"=>"55811354000"}, {"first_name"=>"Xue", "last_name"=>"Zhao", "scopus_author_id"=>"57195232083"}, {"first_name"=>"Guolin", "last_name"=>"Cui", "scopus_author_id"=>"55368703000"}, {"first_name"=>"Fanxia", "last_name"=>"Guo", "scopus_author_id"=>"55649953300"}, {"first_name"=>"Ruiliang", "last_name"=>"Zhu", "scopus_author_id"=>"48663320200"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84925003448", "sgr"=>"84925003448", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0115638", "pmid"=>"25782009", "pui"=>"603051534"}, "id"=>"afbdd42c-75ca-343f-a21e-5f78c0433837", "abstract"=>"Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator. However, the components of TPPPS have not been recognized. To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation. Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography. The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively. Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose. The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2. TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion. By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity. TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro. Results indicated that TPPPS comprised three main components, among which, TPPPS1 mainly showed antioxidant effects, whereas TPPPS2 and TPPPS3 played key roles in immunomodulation, especially TPPPS3. Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator.", "link"=>"http://www.mendeley.com/research/characterization-biological-activity-taishan-pinus-massoniana-pollen-polysaccharide-vitro", "reader_count"=>6, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Agricultural and Biological Sciences"=>4}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Japan"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1952639"], "description"=>"<p>(A) DF1 cells cultured in 24-well plates were infected with 10 μL/well (TCID<sub>50</sub> of 10<sup>3.75</sup>/0.1 mL) ALV-B. After 9 d of culture in MM containing different concentrations of TPPPS or TPPPS1-3, viral titers in the supernatant were directly determined by TCID<sub>50</sub> assays on DF1 cells. PBS treatment wells served as the control. All values shown are presented as means ± SD from three independent experiments. An asterisk indicates that the value of the corresponding group was significantly different from that of the control group (<i>P</i> < 0.05, ANOVA). (B) DF1 cells infected with ALV-B were treated with 50 μg/mL of TPPPS3 at different viral infection periods, and viral titers in the supernatant were directly determined by TCID<sub>50</sub> assays on DF1 cells. Ad is defined as the “adsorption” period; AA is defined as the period “after adsorption”; Al is the period wherein the polysaccharide was always present. PBS treatment wells served as the control. All values shown are presented as means ± SD from three independent experiments. Different superscripts indicate a significant difference (<i>P</i> < 0.05).</p>", "links"=>[], "tags"=>["TPPPS 2. TPPPS 3", "hplc", "Vitro Taishan Pinus massoniana pollen polysaccharide", "virus adsorption interference", "Taishan Pinus massoniana Pollen Polysaccharide", "TPPPS 1", "spleen lymphocyte proliferation", "subgroup B Avian Leukosis virus", "tnf", "il", "TPPPS 2", "TPPPS 3"], "article_id"=>1338688, "categories"=>["Biological Sciences"], "users"=>["Shifa Yang", "Kai Wei", "Fengjuan Jia", "Xue Zhao", "Guolin Cui", "Fanxia Guo", "Ruiliang Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0115638.g006", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_TPPPS1_3_and_TPPPS_on_antiviral_activity_in_vitro_/1338688", "title"=>"Effects of TPPPS1-3 and TPPPS on antiviral activity <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:03:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952637"], "description"=>"<p>Activated splenocytes in 96-well plates were treated with different concentrations of TPPPS1-3 and TPPPP, then IL-2 and TNF in the supernatant was detected by ELISA. PBS treatment wells served as the control, and the values are presented as mean ± SD of five independent experiments. An asterisk indicates that the value of the corresponding group was significantly different from that of the control group (<i>P</i> < 0.05, ANOVA).</p>", "links"=>[], "tags"=>["TPPPS 2. TPPPS 3", "hplc", "Vitro Taishan Pinus massoniana pollen polysaccharide", "virus adsorption interference", "Taishan Pinus massoniana Pollen Polysaccharide", "TPPPS 1", "spleen lymphocyte proliferation", "subgroup B Avian Leukosis virus", "tnf", "il", "TPPPS 2", "TPPPS 3"], "article_id"=>1338686, "categories"=>["Biological Sciences"], "users"=>["Shifa Yang", "Kai Wei", "Fengjuan Jia", "Xue Zhao", "Guolin Cui", "Fanxia Guo", "Ruiliang Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0115638.g004", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_TPPPS1_3_and_TPPPS_on_IL_2_and_TNF_secretion_in_vitro_/1338686", "title"=>"Effects of TPPPS1-3 and TPPPS on IL-2 and TNF secretion <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:03:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952634"], "description"=>"<p>(A) Ion exchange chromatogram of TPPPS on DEAE-cellulose column, eluted with distilled water, 0.01, 0.03, 0.05, 0.1, and 0.3 M NaCl. T1: TPPPS1; T2: TPPPS2; T3: TPPPS3. (B, C, and D) Gel filtration chromatogram of TPPPS1, TPPPS2, and TPPPS3, respectively, on Sephadex G-200 column eluted with 0.1 M NaCl.</p>", "links"=>[], "tags"=>["TPPPS 2. TPPPS 3", "hplc", "Vitro Taishan Pinus massoniana pollen polysaccharide", "virus adsorption interference", "Taishan Pinus massoniana Pollen Polysaccharide", "TPPPS 1", "spleen lymphocyte proliferation", "subgroup B Avian Leukosis virus", "tnf", "il", "TPPPS 2", "TPPPS 3"], "article_id"=>1338683, "categories"=>["Biological Sciences"], "users"=>["Shifa Yang", "Kai Wei", "Fengjuan Jia", "Xue Zhao", "Guolin Cui", "Fanxia Guo", "Ruiliang Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0115638.g001", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Isolation_and_purification_of_polysaccharides_/1338683", "title"=>"Isolation and purification of polysaccharides.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:03:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952636"], "description"=>"<p>Splenocytes were isolated and cultured in 96-well plates. After ConA stimulation, the proliferation was examined by MTT method as described in the Materials and Methods. Stimulus index represents the ratio of absorbance between the experimental group and control group, and the values are presented as mean ± SD from five independent experiments. Different superscripts indicate a significant difference (<i>P</i> < 0.05).</p>", "links"=>[], "tags"=>["TPPPS 2. TPPPS 3", "hplc", "Vitro Taishan Pinus massoniana pollen polysaccharide", "virus adsorption interference", "Taishan Pinus massoniana Pollen Polysaccharide", "TPPPS 1", "spleen lymphocyte proliferation", "subgroup B Avian Leukosis virus", "tnf", "il", "TPPPS 2", "TPPPS 3"], "article_id"=>1338685, "categories"=>["Biological Sciences"], "users"=>["Shifa Yang", "Kai Wei", "Fengjuan Jia", "Xue Zhao", "Guolin Cui", "Fanxia Guo", "Ruiliang Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0115638.g003", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_TPPPS1_3_and_TPPPS_on_splenocyte_proliferation_in_vitro_/1338685", "title"=>"Effects of TPPPS1-3 and TPPPS on splenocyte proliferation <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:03:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952635"], "description"=>"<p>The standard curve was calibrated with T-series dextran (T-500, T-100, T-70, T-40, and T-10) as standard. lgMs = 7.327–0.727 t<sub>R</sub>, R<sup>2</sup> = 0.994. lgMs was defined as the logarithm of the Mw, and t<sub>R</sub> was the retention time. The molecular weight of TPPPS1-3 was estimated by the standard calibration curve.</p>", "links"=>[], "tags"=>["TPPPS 2. TPPPS 3", "hplc", "Vitro Taishan Pinus massoniana pollen polysaccharide", "virus adsorption interference", "Taishan Pinus massoniana Pollen Polysaccharide", "TPPPS 1", "spleen lymphocyte proliferation", "subgroup B Avian Leukosis virus", "tnf", "il", "TPPPS 2", "TPPPS 3"], "article_id"=>1338684, "categories"=>["Biological Sciences"], "users"=>["Shifa Yang", "Kai Wei", "Fengjuan Jia", "Xue Zhao", "Guolin Cui", "Fanxia Guo", "Ruiliang Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0115638.g002", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mw_determination_with_HPGPC_system_for_TPPPS1_3_/1338684", "title"=>"Mw determination with HPGPC system for TPPPS1-3.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:03:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952640"], "description"=>"<p><sup>a</sup> Man, mannose; Rib, ribose; Xyl, xylose, GluA, glucuronic acid; GalA, galactose acid; Glc, glucose; Gal, galactose; Ara, arabinose.</p><p><sup>b</sup> nd, not detected.</p><p>Components of monosaccharide from TPPPS1-3.</p>", "links"=>[], "tags"=>["TPPPS 2. TPPPS 3", "hplc", "Vitro Taishan Pinus massoniana pollen polysaccharide", "virus adsorption interference", "Taishan Pinus massoniana Pollen Polysaccharide", "TPPPS 1", "spleen lymphocyte proliferation", "subgroup B Avian Leukosis virus", "tnf", "il", "TPPPS 2", "TPPPS 3"], "article_id"=>1338689, "categories"=>["Biological Sciences"], "users"=>["Shifa Yang", "Kai Wei", "Fengjuan Jia", "Xue Zhao", "Guolin Cui", "Fanxia Guo", "Ruiliang Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0115638.t001", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Components_of_monosaccharide_from_TPPPS1_3_/1338689", "title"=>"Components of monosaccharide from TPPPS1-3.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-03-17 04:03:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952638"], "description"=>"<p>The supernatant in 96-well plates cultured with splenocytes were collected. NO production was measured spectrophotometrically using the Griess reaction. PBS treatment wells served as the control, and the values are presented as mean ± SD from five independent experiments. Different superscripts indicate a significant difference (<i>P</i> < 0.05).</p>", "links"=>[], "tags"=>["TPPPS 2. TPPPS 3", "hplc", "Vitro Taishan Pinus massoniana pollen polysaccharide", "virus adsorption interference", "Taishan Pinus massoniana Pollen Polysaccharide", "TPPPS 1", "spleen lymphocyte proliferation", "subgroup B Avian Leukosis virus", "tnf", "il", "TPPPS 2", "TPPPS 3"], "article_id"=>1338687, "categories"=>["Biological Sciences"], "users"=>["Shifa Yang", "Kai Wei", "Fengjuan Jia", "Xue Zhao", "Guolin Cui", "Fanxia Guo", "Ruiliang Zhu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0115638.g005", "stats"=>{"downloads"=>2, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_TPPPS1_3_and_TPPPS_on_production_of_NO_in_vitro_/1338687", "title"=>"Effects of TPPPS1-3 and TPPPS on production of NO <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:03:04"}

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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