Generation of BAC Transgenic Tadpoles Enabling Live Imaging of Motoneurons by Using the Urotensin II-Related Peptide (ust2b) Gene as a Driver
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Generation of BAC transgenic tadpoles enabling live imaging of motoneurons by using the urotensin II-related peptide (ust2b) gene as a driver", "type"=>"journal", "authors"=>[{"first_name"=>"Marion", "last_name"=>"Bougerol", "scopus_author_id"=>"55151558600"}, {"first_name"=>"Frédéric", "last_name"=>"Auradé", "scopus_author_id"=>"6506645590"}, {"first_name"=>"François M.", "last_name"=>"Lambert", "scopus_author_id"=>"56124964300"}, {"first_name"=>"Didier", "last_name"=>"Le Ray", "scopus_author_id"=>"7006785259"}, {"first_name"=>"Denis", "last_name"=>"Combes", "scopus_author_id"=>"7006006533"}, {"first_name"=>"Muriel", "last_name"=>"Thoby-Brisson", "scopus_author_id"=>"6602348950"}, {"first_name"=>"Frédéric", "last_name"=>"Relaix", "scopus_author_id"=>"6602850818"}, {"first_name"=>"Nicolas", "last_name"=>"Pollet", "scopus_author_id"=>"6603710250"}, {"first_name"=>"Hervé", "last_name"=>"Tostivint", "scopus_author_id"=>"35447989400"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84922569401", "doi"=>"10.1371/journal.pone.0117370", "issn"=>"19326203", "pui"=>"602121414", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"25658845", "scopus"=>"2-s2.0-84922569401"}, "id"=>"f6ff0c6f-f7e1-353c-b453-c12325c0aca2", "abstract"=>"Xenopus is an excellent tetrapod model for studying normal and pathological motoneuron ontogeny due to its developmental morpho-physiological advantages. In mammals, the urotensin II-related peptide (UTS2B) gene is primarily expressed in motoneurons of the brainstem and the spinal cord. Here, we show that this expression pattern was conserved in Xenopus and established during the early embryonic development, starting at the early tailbud stage. In late tadpole stage, uts2b mRNA was detected both in the hindbrain and in the spinal cord. Spinal uts2b+ cells were identified as axial motoneurons. In adult, however, the uts2b expression was only detected in the hindbrain. We assessed the ability of the uts2b promoter to drive the expression of a fluorescent reporter in motoneurons by recombineering a green fluorescent protein (GFP) into a bacterial artificial chromosome (BAC) clone containing the entire X. tropicalis uts2b locus. After injection of this construction in one-cell stage embryos, a transient GFP expression was observed in the spinal cord of about a quarter of the resulting animals from the early tailbud stage and up to juveniles. The GFP expression pattern was globally consistent with that of the endogenous uts2b in the spinal cord but no fluorescence was observed in the brainstem. A combination of histological and electrophysiological approaches was employed to further characterize the GFP+ cells in the larvae. More than 98% of the GFP+ cells expressed choline acetyltransferase, while their projections were co-localized with alpha-bungarotoxin labeling. When tail myotomes were injected with rhodamine dextran amine crystals, numerous double-stained GFP+ cells were observed. In addition, intracellular electrophysiological recordings of GFP+ neurons revealed locomotion-related rhythmic discharge patterns during fictive swimming. Taken together our results provide evidence that uts2b is an appropriate driver to express reporter genes in larval motoneurons of the Xenopus spinal cord.", "link"=>"http://www.mendeley.com/research/generation-bac-transgenic-tadpoles-enabling-live-imaging-motoneurons-using-urotensin-iirelated-pepti", "reader_count"=>15, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>3, "Student > Bachelor"=>3, "Professor"=>3}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>3, "Student > Bachelor"=>3, "Professor"=>3}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>6, "Neuroscience"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>1, "Sports and Recreations"=>1, "Unspecified"=>1}, "reader_count_by_subdiscipline"=>{"Neuroscience"=>{"Neuroscience"=>3}, "Chemistry"=>{"Chemistry"=>1}, "Sports and Recreations"=>{"Sports and Recreations"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United Kingdom"=>1}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84922569401"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84922569401?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84922569401&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84922569401&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84922569401", "dc:identifier"=>"SCOPUS_ID:84922569401", "eid"=>"2-s2.0-84922569401", "dc:title"=>"Generation of BAC transgenic tadpoles enabling live imaging of motoneurons by using the urotensin II-related peptide (ust2b) gene as a driver", "dc:creator"=>"Bougerol M.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-02-06", "prism:coverDisplayDate"=>"6 February 2015", "prism:doi"=>"10.1371/journal.pone.0117370", "citedby-count"=>"3", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Museum National d'Histoire Naturelle", "affiliation-city"=>"Paris", "affiliation-country"=>"France"}], "pubmed-id"=>"25658845", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0117370", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0117370", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"47", "xml_views"=>"16", "html_views"=>"311"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"71"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"31"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"132"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"87"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"3", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1884364"], "description"=>"<p>Visualization of spinal GFP<sup>+</sup> neurons at x40 in fluorescence condition (<b>A1</b>) and infrared condition (<b>A2</b>) in a whole-mount isolated <i>in vitro</i> preparation of brainstem-spinal cord with dorsal-side opened. Patch-clamp intracellular recordings (bottom traces) of spinal GFP<sup>+</sup> neurons (Intra.) either with a persisting (<b>B1</b>), a non-persisting firing pattern (<b>B2</b>), or a slow-delayed firing pattern (<b>B3</b>) during spontaneous fictive swimming episodes (f. sw.). The upper trace in <b>B1</b>–<b>B3</b> represents the extracellular fictive swimming activity recorded simultaneously in a ventral motor root (Vr). DIC, differential interference contrast. The white arrowhead points to the GFP<sup>+</sup> neuron targeted for patch clamp recording.</p>", "links"=>[], "tags"=>["ust 2b Gene", "Xenopu", "tailbud stage", "rhodamine dextran amine crystals", "BAC Transgenic Tadpoles Enabling Live Imaging", "uts 2b mRNA", "uts 2b expression", "uts 2b", "intracellular electrophysiological recordings", "Spinal uts 2b cells", "GFP expression pattern", "uts 2b promoter", "tropicalis uts 2b locus", "motoneuron", "UTS 2B gene"], "article_id"=>1303227, "categories"=>["Uncategorised"], "users"=>["Marion Bougerol", "Frédéric Auradé", "François M. Lambert", "Didier Le Ray", "Denis Combes", "Muriel Thoby-Brisson", "Frederic Relaix", "Nicolas Pollet", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117370.g010", "stats"=>{"downloads"=>4, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_GFP_cells_exhibit_a_typical_pattern_of_motoneuronal_electrical_activity_during_spontaneous_fictive_swimming_episodes_/1303227", "title"=>"GFP<sup>+</sup> cells exhibit a typical pattern of motoneuronal electrical activity during spontaneous fictive swimming episodes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 04:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1884346"], "description"=>"<p>Distribution of the <i>uts2b</i> mRNA revealed by <i>in situ</i> hybridization with <i>uts2b</i> antisense probe on whole tadpoles (<b>A</b>–<b>D</b> and <b>H</b>), dissected and opened CNS (<b>E</b>), or CNS sections (<b>F</b>, <b>G</b>) at stages 24 (<b>A</b>), 27 (<b>B</b>), 33/34 (<b>C</b>), 37/38 (<b>D</b>) 51 (<b>E</b>), and 55 (<b>F</b>, <b>G</b>). <b>H</b>. Stage 37/38 tadpole hybridized with <i>uts2b</i> sense probe used as negative control. <b>A</b>–<b>D</b> and <b>H</b>. Lateral views of tadpoles with dorsal side up and rostral to the right. <b>E</b>. Dorsal view of whole-mount preparation of hindbrain-spinal cord (CNS). Note that in this preparation, the more ventral structures are closer to the midline. <b>F</b> and <b>G</b>. Coronal sections at the level of the spinal cord (<b>F</b>) and the posterior part of brainstem (<b>G</b>) with dorsal up. Arrowheads point to <i>uts2b</i> mRNA detection in stage 24 tadpole and in F and G coronal sections. White arrows designate the region of nuclei of the trigeminal (V) nerve; asterisks denote the region of nuclei of the vagus/hypoglossal/accessory (IX–XI) nerves. r, rhombomere; V4, fourth ventricule.</p>", "links"=>[], "tags"=>["ust 2b Gene", "Xenopu", "tailbud stage", "rhodamine dextran amine crystals", "BAC Transgenic Tadpoles Enabling Live Imaging", "uts 2b mRNA", "uts 2b expression", "uts 2b", "intracellular electrophysiological recordings", "Spinal uts 2b cells", "GFP expression pattern", "uts 2b promoter", "tropicalis uts 2b locus", "motoneuron", "UTS 2B gene"], "article_id"=>1303209, "categories"=>["Uncategorised"], "users"=>["Marion Bougerol", "Frédéric Auradé", "François M. Lambert", "Didier Le Ray", "Denis Combes", "Muriel Thoby-Brisson", "Frederic Relaix", "Nicolas Pollet", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117370.g002", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_uts2b_mRNA_is_restricted_to_the_ventral_spinal_cord_and_hindbrainin_X_laevis_tadpoles_/1303209", "title"=>"<i>uts2b</i> mRNA is restricted to the ventral spinal cord and hindbrainin <i>X. laevis</i> tadpoles.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 04:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1884363"], "description"=>"<p>Confocal image of combined immuno-labeling of spinal GFP<sup>+</sup> neurons (<b>A1</b>) and retrograde labeling of spinal motoneurons (<b>A2</b>) in a stage 50 <i>uts2b</i>-GFP tadpole whole-mount spinal cord preparation. <b>A3</b>. Merged image obtained when GFP staining and retrograde labeling were superimposed. Dorsal view with rostral up. Only the hemi-cord is shown. The dashed-line on the right side represents the midline. Axial motoneurons (Ax MN) innervating myotomes were labeled from tail muscles with rhodamine (RDA) dextran dye whereas appendicular motoneurons (Ap MN) innervating limbs were labeled from hindlimb buds with alexa dextran 647 dye (A.D. 647; see scheme on the left panel). The drawing at the top left panel illustrates the medio-lateral localization of Ax MN and developing Ap MN in larval spinal cord at stage 50. Yellow arrowheads indicate double stained cells. Cross-section confocal image of combined immuno-labeling of spinal GFP<sup>+</sup> neurons (<b>B1</b>, <b>C1</b>) and retrograde labeling of dorsal thoracic motoneurons (dTh MN, <b>B2</b>) and Ap MN, (<b>C2</b>) in stage 60 metamorphosing <i>uts2b</i>-GFP tadpole. <b>B3</b>, <b>C3</b>. Merged images obtained GFP staining and retrograde labeling were superimposed. dTh MN were labeled from <i>dorsalis trunci</i> muscles with RDA whereas Ap MN were labeled from groups of extensor and flexor of the hindlimb with A.D. 647 (see scheme on the left side). The cross-section drawing at the bottom left panel illustrates the dorso-ventral localization of dTh MN and Ap MN in the future adult spinal cord at stage 60. sp.sgt 7–10, spinal segments 7 to 10, Vr, ventral root. D, dorsal; L, lateral.</p>", "links"=>[], "tags"=>["ust 2b Gene", "Xenopu", "tailbud stage", "rhodamine dextran amine crystals", "BAC Transgenic Tadpoles Enabling Live Imaging", "uts 2b mRNA", "uts 2b expression", "uts 2b", "intracellular electrophysiological recordings", "Spinal uts 2b cells", "GFP expression pattern", "uts 2b promoter", "tropicalis uts 2b locus", "motoneuron", "UTS 2B gene"], "article_id"=>1303226, "categories"=>["Uncategorised"], "users"=>["Marion Bougerol", "Frédéric Auradé", "François M. Lambert", "Didier Le Ray", "Denis Combes", "Muriel Thoby-Brisson", "Frederic Relaix", "Nicolas Pollet", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117370.g009", "stats"=>{"downloads"=>2, "page_views"=>54, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Spinal_GFP_cells_of_transgenic_uts2b_GFP_X_laevis_tadpoles_project_to_tail_myotomes_/1303226", "title"=>"Spinal GFP<sup>+</sup> cells of transgenic <i>uts2b</i>-GFP <i>X. laevis</i> tadpoles project to tail myotomes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 04:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1884343"], "description"=>"<p>The <i>X. tropicalis uts2b</i> BAC clone, ALNOAAA14YB24, contains approximately 164 kbp genomic DNA. The <i>uts2b</i> gene is approximately 8.2 kbp long as represented by a red arrow, and it comprises 5 exons. The targeting vector was designed to contain two <i>uts2b</i> genomic DNAs on its either side (denoted 5’- and 3’-arms), that allows a specific homologous recombination between the targeting vector and the <i>uts2b</i> BAC DNA at the genomic region surrounding the first exon. Homology arms for the first exon of <i>uts2b</i> were ligated to both ends of an EGFP.FRT-Promotor Bacterial Minimal.kanamycin-FRT.2pA expression cassette. Through the first homologous recombination, the expression cassette was inserted in place of the first <i>uts2b</i> exon in the <i>uts2b</i> BAC clone. Then, the kanamycin resistance cassette was selectively eliminated by expressing Flipase, leading to the <i>uts2b</i>-EGFP BAC clone containing only the EGFP cDNA expression cassette under control of the <i>uts2b</i> promoter. Finally, note that the <i>ccdc50</i> gene, located close to the <i>uts2b</i> locus, was deleted from the <i>uts2b</i> BAC to avoid possible artifacts due to its overexpression (not shown).</p>", "links"=>[], "tags"=>["ust 2b Gene", "Xenopu", "tailbud stage", "rhodamine dextran amine crystals", "BAC Transgenic Tadpoles Enabling Live Imaging", "uts 2b mRNA", "uts 2b expression", "uts 2b", "intracellular electrophysiological recordings", "Spinal uts 2b cells", "GFP expression pattern", "uts 2b promoter", "tropicalis uts 2b locus", "motoneuron", "UTS 2B gene"], "article_id"=>1303206, "categories"=>["Uncategorised"], "users"=>["Marion Bougerol", "Frédéric Auradé", "François M. Lambert", "Didier Le Ray", "Denis Combes", "Muriel Thoby-Brisson", "Frederic Relaix", "Nicolas Pollet", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117370.g001", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Strategy_for_constructing_the_recombinant_uts2b_BAC_containing_uts2b_EGFP_expression_cassette_/1303206", "title"=>"Strategy for constructing the recombinant <i>uts2b</i> BAC containing <i>uts2b</i>-EGFP expression cassette.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 04:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1884351"], "description"=>"<p>Confocal image of combined fluorescent <i>in situ</i> hybridization of <i>uts2b</i> mRNA (<b>A1</b>, <b>B1</b>) and retrograde labeling of spinal axial (<b>A2</b>) and appendicular (<b>B2</b>) motoneurons in stage 55 tadpole whole-mount spinal cord preparations. <b>A3</b>, <b>B3</b>. Merged images of <i>uts2b</i> staining and retrograde labeling. All images display dorsal view of hemi-cords, with the rostral side up (dashed-line on the left indicates the midline). Axial motoneurons (Ax MN) were labeled with rhodamine dextran dye (RDA) injected into tail myotomes while appendicular motoneurons (Ap MN) were labeled from posterior leg muscles with Alexa Dextran 647 dye (A.D. 647; see upper scheme on the left panel). The drawing on the left panel illustrates the localization of Ax MN and developing Ap MN in larval spinal cord at stage 55. Yellow arrowheads indicate double stained cells. Red arrowheads indicate <i>uts2b—</i>retrograde labeled cells. sp.sgt 7–10, spinal segments 7 to 10.</p>", "links"=>[], "tags"=>["ust 2b Gene", "Xenopu", "tailbud stage", "rhodamine dextran amine crystals", "BAC Transgenic Tadpoles Enabling Live Imaging", "uts 2b mRNA", "uts 2b expression", "uts 2b", "intracellular electrophysiological recordings", "Spinal uts 2b cells", "GFP expression pattern", "uts 2b promoter", "tropicalis uts 2b locus", "motoneuron", "UTS 2B gene"], "article_id"=>1303215, "categories"=>["Uncategorised"], "users"=>["Marion Bougerol", "Frédéric Auradé", "François M. Lambert", "Didier Le Ray", "Denis Combes", "Muriel Thoby-Brisson", "Frederic Relaix", "Nicolas Pollet", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117370.g004", "stats"=>{"downloads"=>3, "page_views"=>35, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Spinal_uts2b_cells_of_X_laevis_tadpoles_project_to_tail_myotomes_/1303215", "title"=>"Spinal <i>uts2b</i><sup>+</sup> cells of <i>X. laevis</i> tadpoles project to tail myotomes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 04:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1884353"], "description"=>"<p><b>A</b>. GFP fluorescence imaging of a representative transgenic tadpole at stage 58. The tail is seen in lateral view while the head is seen in dorsal view. <b>B</b>. GFP expression at the level of a dissected and opened whole-mount CNS of a stage 50 transgenic tadpole. The CNS was optically exposed by removing the dorsal part of the tail and the top of the head. Note that GFP<sup>+</sup> cells are restricted to the spinal cord. Dorsal view, rostral to the right. <b>C</b>. Detail of <b>A</b> showing GFP<sup>+</sup> motor axon projections (arrowheads) extending towards axial musculature. White dashed vertical lines indicate myotome boundaries. SC, spinal cord. OT, optic tectum.</p>", "links"=>[], "tags"=>["ust 2b Gene", "Xenopu", "tailbud stage", "rhodamine dextran amine crystals", "BAC Transgenic Tadpoles Enabling Live Imaging", "uts 2b mRNA", "uts 2b expression", "uts 2b", "intracellular electrophysiological recordings", "Spinal uts 2b cells", "GFP expression pattern", "uts 2b promoter", "tropicalis uts 2b locus", "motoneuron", "UTS 2B gene"], "article_id"=>1303216, "categories"=>["Uncategorised"], "users"=>["Marion Bougerol", "Frédéric Auradé", "François M. Lambert", "Didier Le Ray", "Denis Combes", "Muriel Thoby-Brisson", "Frederic Relaix", "Nicolas Pollet", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117370.g005", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Most_of_the_fluorescence_visible_in_transgenic_uts2b_GFP_X_laevis_tadpoles_occurs_in_cells_located_in_the_spinal_cord_and_in_motor_axon_projections_/1303216", "title"=>"Most of the fluorescence visible in transgenic <i>uts2b</i>-GFP <i>X. laevis</i> tadpoles occurs in cells located in the spinal cord and in motor axon projections.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 04:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1884365"], "description"=>"<div><p><i>Xenopus</i> is an excellent tetrapod model for studying normal and pathological motoneuron ontogeny due to its developmental morpho-physiological advantages. In mammals, the urotensin II-related peptide (<i>UTS2B</i>) gene is primarily expressed in motoneurons of the brainstem and the spinal cord. Here, we show that this expression pattern was conserved in <i>Xenopus</i> and established during the early embryonic development, starting at the early tailbud stage. In late tadpole stage, <i>uts2b</i> mRNA was detected both in the hindbrain and in the spinal cord. Spinal <i>uts2b<sup>+</sup></i> cells were identified as axial motoneurons. In adult, however, the <i>uts2b</i> expression was only detected in the hindbrain. We assessed the ability of the <i>uts2b</i> promoter to drive the expression of a fluorescent reporter in motoneurons by recombineering a green fluorescent protein (GFP) into a bacterial artificial chromosome (BAC) clone containing the entire <i>X. tropicalis uts2b</i> locus. After injection of this construction in one-cell stage embryos, a transient GFP expression was observed in the spinal cord of about a quarter of the resulting animals from the early tailbud stage and up to juveniles. The GFP expression pattern was globally consistent with that of the endogenous <i>uts2b</i> in the spinal cord but no fluorescence was observed in the brainstem. A combination of histological and electrophysiological approaches was employed to further characterize the GFP<sup>+</sup> cells in the larvae. More than 98% of the GFP<sup>+</sup> cells expressed choline acetyltransferase, while their projections were co-localized with α-bungarotoxin labeling. When tail myotomes were injected with rhodamine dextran amine crystals, numerous double-stained GFP<sup>+</sup> cells were observed. In addition, intracellular electrophysiological recordings of GFP<sup>+</sup> neurons revealed locomotion-related rhythmic discharge patterns during fictive swimming. Taken together our results provide evidence that <i>uts2b</i> is an appropriate driver to express reporter genes in larval motoneurons of the <i>Xenopus</i> spinal cord.</p></div>", "links"=>[], "tags"=>["ust 2b Gene", "Xenopu", "tailbud stage", "rhodamine dextran amine crystals", "BAC Transgenic Tadpoles Enabling Live Imaging", "uts 2b mRNA", "uts 2b expression", "uts 2b", "intracellular electrophysiological recordings", "Spinal uts 2b cells", "GFP expression pattern", "uts 2b promoter", "tropicalis uts 2b locus", "motoneuron", "UTS 2B gene"], "article_id"=>1303228, "categories"=>["Uncategorised"], "users"=>["Marion Bougerol", "Frédéric Auradé", "François M. Lambert", "Didier Le Ray", "Denis Combes", "Muriel Thoby-Brisson", "Frederic Relaix", "Nicolas Pollet", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117370", "stats"=>{"downloads"=>6, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Generation_of_BAC_Transgenic_Tadpoles_Enabling_Live_Imaging_of_Motoneurons_by_Using_the_Urotensin_II_Related_Peptide_ust2b_Gene_as_a_Driver/1303228", "title"=>"Generation of BAC Transgenic Tadpoles Enabling Live Imaging of Motoneurons by Using the Urotensin II-Related Peptide (<i>ust2b</i>) Gene as a Driver", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-02-06 04:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1884362"], "description"=>"<p>Combined fluorescence of GFP (<b>A</b>) and immuno-labeling of α-bungarotoxin (<b>B</b>) at the level of spinal axon motor projections in a whole-mount stage 57 transgenic tadpole. <b>C</b>. Merged image obtained when GFP fluorescence and α-bungarotoxin staining were superimposed. Postsynaptic nicotinic acetylcholine receptors labeled by α-bungarotoxin and GFP<sup>+</sup> motor neuron axons overlap (arrowheads). Lateral views, rostral to the left.</p>", "links"=>[], "tags"=>["ust 2b Gene", "Xenopu", "tailbud stage", "rhodamine dextran amine crystals", "BAC Transgenic Tadpoles Enabling Live Imaging", "uts 2b mRNA", "uts 2b expression", "uts 2b", "intracellular electrophysiological recordings", "Spinal uts 2b cells", "GFP expression pattern", "uts 2b promoter", "tropicalis uts 2b locus", "motoneuron", "UTS 2B gene"], "article_id"=>1303225, "categories"=>["Uncategorised"], "users"=>["Marion Bougerol", "Frédéric Auradé", "François M. Lambert", "Didier Le Ray", "Denis Combes", "Muriel Thoby-Brisson", "Frederic Relaix", "Nicolas Pollet", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117370.g008", "stats"=>{"downloads"=>2, "page_views"=>37, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Spinal_GFP_axon_motor_projections_of_transgenic_uts2b_GFP_X_laevis_tadpoles_co_localize_with_bungarotoxin_a_marker_of_postsynaptic_neuromuscular_junctions_/1303225", "title"=>"Spinal GFP<sup>+</sup> axon motor projections of transgenic <i>uts2b</i>-GFP <i>X. laevis</i> tadpoles co-localize with α-bungarotoxin, a marker of postsynaptic neuromuscular junctions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 04:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1884347"], "description"=>"<p>Confocal images of combined fluorescent in situ hybridization of uts2b mRNA (<b>A1</b>, <b>B1</b>) and ChAT immunolabeling (<b>A2</b>, <b>B2</b>) in a whole-mount dissected and opened spinal cord preparation of stage 50 wild type tadpole. <b>A3</b>, <b>B3</b>. Merged image obtained when uts2b and ChAT stainings were superimposed. Dorsal view with rostral up. Note that in this preparation, the more ventral structures are close to the midline (dashed line). The boxed region in <b>A3</b> is shown at higher magnification in <b>B1</b>–<b>B3</b>.</p>", "links"=>[], "tags"=>["ust 2b Gene", "Xenopu", "tailbud stage", "rhodamine dextran amine crystals", "BAC Transgenic Tadpoles Enabling Live Imaging", "uts 2b mRNA", "uts 2b expression", "uts 2b", "intracellular electrophysiological recordings", "Spinal uts 2b cells", "GFP expression pattern", "uts 2b promoter", "tropicalis uts 2b locus", "motoneuron", "UTS 2B gene"], "article_id"=>1303210, "categories"=>["Uncategorised"], "users"=>["Marion Bougerol", "Frédéric Auradé", "François M. Lambert", "Didier Le Ray", "Denis Combes", "Muriel Thoby-Brisson", "Frederic Relaix", "Nicolas Pollet", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117370.g003", "stats"=>{"downloads"=>2, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Spinal_uts2b_cells_are_cholinergic_neurons_in_X_laevis_tadpoles_/1303210", "title"=>"Spinal <i>uts2b</i><sup>+</sup> cells are cholinergic neurons in <i>X. laevis</i> tadpoles.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 04:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1884359"], "description"=>"<p>Confocal images of combined immuno-labeling of ChAT<sup>+</sup> (<b>A1</b>) and GFP<sup>+</sup> (<b>A2</b>) neurons in a whole-mount spinal cord preparation of stage 50 transgenic tadpole. <b>A3</b>. Merged image obtained when ChAT and GFP stainings were superimposed. Dorsal view with rostral up. Only the hemi-cord is shown. The dashed-line on the right side represents the midline. Note that in this preparation, the more ventral structures are closer to the midline. Confocal images of combined immuno-labeling of ChAT<sup>+</sup> (<b>B1</b>–<b>C1</b>) and GFP<sup>+</sup> (<b>B2</b>–<b>C2</b>) neurons in lumbar spinal cord (<b>B</b>) and brainstem (<b>C</b>) cross-sections of stage 60 <i>uts2b</i>-GFP tadpoles. <b>B3</b>, <b>C3</b>. Merged images obtained when ChAT and GFP stainings were superimposed. The cross-section in <b>C1</b>–<b>3</b> originates from the caudal hindbrain (rhombomeres 7–8) where IX-XI motor nuclei are located. Yellow arrowheads designate GFP<sup>+</sup>/ChAT<sup>+</sup> cells; green arrowheads designate GFP<sup>+</sup>/ChAT<sup>−</sup> cells; White arrowheads designate GFP<sup>−</sup>/ChAT<sup>+</sup> cells. D, dorsal; M, medial; V4, 4<sup>th</sup> ventricule.</p>", "links"=>[], "tags"=>["ust 2b Gene", "Xenopu", "tailbud stage", "rhodamine dextran amine crystals", "BAC Transgenic Tadpoles Enabling Live Imaging", "uts 2b mRNA", "uts 2b expression", "uts 2b", "intracellular electrophysiological recordings", "Spinal uts 2b cells", "GFP expression pattern", "uts 2b promoter", "tropicalis uts 2b locus", "motoneuron", "UTS 2B gene"], "article_id"=>1303222, "categories"=>["Uncategorised"], "users"=>["Marion Bougerol", "Frédéric Auradé", "François M. Lambert", "Didier Le Ray", "Denis Combes", "Muriel Thoby-Brisson", "Frederic Relaix", "Nicolas Pollet", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117370.g007", "stats"=>{"downloads"=>2, "page_views"=>37, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Spinal_GFP_cells_of_transgenic_uts2b_GFP_X_laevis_tadpoles_mainly_express_ChAT_/1303222", "title"=>"Spinal GFP<sup>+</sup> cells of transgenic <i>uts2b</i>-GFP <i>X. laevis</i> tadpoles mainly express ChAT.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 04:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1884355"], "description"=>"<p>Confocal images of combined immuno-labeling of GFP<sup>+</sup> neurons (<b>A1</b>, <b>B1</b>) and fluorescent <i>in situ</i> hybridization of <i>uts2b</i> mRNA (<b>A2</b>, <b>B2</b>) in a whole-mount spinal cord preparation of stage 50 <i>uts2b</i>-GFP tadpole. <b>A3</b>, <b>B3</b>. Merged image obtained when GFP and <i>uts2b</i> stainings were superimposed. Dorsal view with rostral up. Only the hemi-cord is shown. The dashed-line on the left side represents the midline. Note that in this preparation, the more ventral structures are closer to the midline. The boxed region in <b>A3</b> is shown at higher magnification in <b>B1</b>–<b>B3</b>. Yellow arrowheads denote cells that express both GFP and <i>uts2b</i>. Red arrowheads designate <i>uts2b</i><sup>+</sup>/GFP<sup>−</sup> cells and green arrowheads designate <i>uts2b</i><sup>−</sup>/GFP<sup>+</sup> cells.</p>", "links"=>[], "tags"=>["ust 2b Gene", "Xenopu", "tailbud stage", "rhodamine dextran amine crystals", "BAC Transgenic Tadpoles Enabling Live Imaging", "uts 2b mRNA", "uts 2b expression", "uts 2b", "intracellular electrophysiological recordings", "Spinal uts 2b cells", "GFP expression pattern", "uts 2b promoter", "tropicalis uts 2b locus", "motoneuron", "UTS 2B gene"], "article_id"=>1303218, "categories"=>["Uncategorised"], "users"=>["Marion Bougerol", "Frédéric Auradé", "François M. Lambert", "Didier Le Ray", "Denis Combes", "Muriel Thoby-Brisson", "Frederic Relaix", "Nicolas Pollet", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117370.g006", "stats"=>{"downloads"=>2, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_uts2b_expression_pattern_is_partially_reproduced_by_GFP_in_transgenic_uts2b_GFP_X_laevis_tadpoles_/1303218", "title"=>"The <i>uts2b</i> expression pattern is partially reproduced by GFP in transgenic <i>uts2b</i>-GFP <i>X. laevis</i> tadpoles.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 04:47:43"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"18", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"19", "full-text"=>"27", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"21", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts