Isonitrosoacetophenone Drives Transcriptional Reprogramming in Nicotiana tabacum Cells in Support of Innate Immunity and Defense
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Isonitrosoacetophenone drives transcriptional reprogramming in Nicotiana tabacum cells in support of innate immunity and defense", "type"=>"journal", "authors"=>[{"first_name"=>"Arnaud T.", "last_name"=>"Djami-Tchatchou", "scopus_author_id"=>"37761353200"}, {"first_name"=>"Mmapula P.", "last_name"=>"Maake", "scopus_author_id"=>"56512427800"}, {"first_name"=>"Lizelle A.", "last_name"=>"Piater", "scopus_author_id"=>"7801521914"}, {"first_name"=>"Ian A.", "last_name"=>"Dubery", "scopus_author_id"=>"7003428879"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84922572544", "doi"=>"10.1371/journal.pone.0117377", "scopus"=>"2-s2.0-84922572544", "pui"=>"602121413", "issn"=>"19326203"}, "id"=>"90d7d1f6-89b6-36fc-9d3f-23a9c9b1a778", "abstract"=>"Plants respond to various stress stimuli by activating broad-spectrum defense responses both locally as well as systemically. As such, identification of expressed genes represents an important step towards understanding inducible defense responses and assists in de- signing appropriate intervention strategies for disease management. Genes differentially expressed in tobacco cell suspensions following elicitation with isonitrosoacetophenone (INAP) were identified using mRNA differential display and pyro-sequencing. Sequencing data produced 14579 reads, which resulted in 198 contigs and 1758 singletons. Following BLAST analyses, several inducible plant defense genes of interest were identified and clas- sified into functional categories including signal transduction, transcription activation, tran- scription and protein synthesis, protein degradation and ubiquitination, stress-responsive, defense-related, metabolism and energy, regulation, transportation, cytoskeleton and cell wall-related. Quantitative PCR was used to investigate the expression of 17 selected target genes within these categories. Results indicate that INAP has a sensitising or priming effect through activation of salicylic acid-, jasmonic acid- and ethylene pathways that result in an altered transcriptome, with the expression of genes involved in perception of pathogens and associated cellular re-programming in support of defense. Furthermore, infection as- says with the pathogen Pseudomonas syringae pv. tabaci confirmed the establishment of a functional anti-microbial environment in planta.", "link"=>"http://www.mendeley.com/research/isonitrosoacetophenone-drives-transcriptional-reprogramming-nicotiana-tabacum-cells-support-innate-i", "reader_count"=>15, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Master"=>5, "Professor"=>1, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Master"=>5, "Professor"=>1, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>12, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Social Sciences"=>{"Social Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>12}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84922572544"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84922572544?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84922572544&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84922572544&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84922572544", "dc:identifier"=>"SCOPUS_ID:84922572544", "eid"=>"2-s2.0-84922572544", "dc:title"=>"Isonitrosoacetophenone drives transcriptional reprogramming in Nicotiana tabacum cells in support of innate immunity and defense", "dc:creator"=>"Djami-Tchatchou A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-02-06", "prism:coverDisplayDate"=>"6 February 2015", "prism:doi"=>"10.1371/journal.pone.0117377", "citedby-count"=>"7", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Johannesburg", "affiliation-city"=>"Johannesburg", "affiliation-country"=>"South Africa"}], "pubmed-id"=>"25658943", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0117377", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0117377", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"45", "xml_views"=>"18", "html_views"=>"362"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"129"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"17"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"4", "html_views"=>"3"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"0"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"0"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"0"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1883487"], "description"=>"<p><i>tabaci</i> in tobacco leaf tissues following pre-conditioning with 1 mM INAP. Graphical representation of bacterial cell counts expressed as colony forming units (CFUs) per square cm obtained from serially diluted extracts after 2 days of growth on King’s B medium. C = Controls, infiltrated with 10 mM MgSO<sub>4</sub> and T = INAP conditioned tissue, infiltrated with 1 mM INAP dissolved in 10 mM MgSO<sub>4</sub>. Error barrs indicate standard deviation of three biological repeats.</p>", "links"=>[], "tags"=>["Nicotiana tabacum Cells", "sequencing data", "INAP", "Signal transduction", "Innate immunity", "BLAST analyses", "198 contigs", "infection assays", "inducible plant defense genes", "acid", "target genes", "1758 singletons", "expression", "stress stimuli", "genes differentially", "disease management", "Quantitative PCR", "protein synthesis", "understanding inducible defense responses", "Defense Plants", "intervention strategies", "tobacco cell suspensions", "category", "protein degradation", "Isonitrosoacetophenone Drives Transcriptional Reprogramming", "ethylene pathways", "pathogen Pseudomonas syringae pv", "transcription activation"], "article_id"=>1302541, "categories"=>["Uncategorised"], "users"=>["Arnaud T. Djami-Tchatchou", "Mmapula P. Maake", "Lizelle A. Piater", "Ian A. Dubery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117377.g004", "stats"=>{"downloads"=>0, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_planta_growth_of_Pseudomonas_syringae_pv_/1302541", "title"=>"<i>In planta</i> growth of <i>Pseudomonas syringae</i> pv.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 03:44:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/1883486"], "description"=>"<p>Results show a broad activation of cellular responses involved in innate immunity from pathogen or PAMP perception to the eventual enhanced innate immune response. Details of the gene transcripts found to be up-regulated in response to treatment of cultured cells by INAP are given in the main text.</p>", "links"=>[], "tags"=>["Nicotiana tabacum Cells", "sequencing data", "INAP", "Signal transduction", "Innate immunity", "BLAST analyses", "198 contigs", "infection assays", "inducible plant defense genes", "acid", "target genes", "1758 singletons", "expression", "stress stimuli", "genes differentially", "disease management", "Quantitative PCR", "protein synthesis", "understanding inducible defense responses", "Defense Plants", "intervention strategies", "tobacco cell suspensions", "category", "protein degradation", "Isonitrosoacetophenone Drives Transcriptional Reprogramming", "ethylene pathways", "pathogen Pseudomonas syringae pv", "transcription activation"], "article_id"=>1302540, "categories"=>["Uncategorised"], "users"=>["Arnaud T. Djami-Tchatchou", "Mmapula P. Maake", "Lizelle A. Piater", "Ian A. Dubery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117377.g003", "stats"=>{"downloads"=>1, "page_views"=>40, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_diagram_illustrating_the_priming_action_of_INAP_on_N_tabacum_cells_/1302540", "title"=>"Schematic diagram illustrating the priming action of INAP on <i>N. tabacum</i> cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 03:44:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/1883483"], "description"=>"<p>Pie-chart showing genes induced by INAP treatment in cultured tobacco cells, identified through ACP-DDRT-PCR, pyro-sequencing and BLAST analyses, as expressed in percentage values.</p>", "links"=>[], "tags"=>["Nicotiana tabacum Cells", "sequencing data", "INAP", "Signal transduction", "Innate immunity", "BLAST analyses", "198 contigs", "infection assays", "inducible plant defense genes", "acid", "target genes", "1758 singletons", "expression", "stress stimuli", "genes differentially", "disease management", "Quantitative PCR", "protein synthesis", "understanding inducible defense responses", "Defense Plants", "intervention strategies", "tobacco cell suspensions", "category", "protein degradation", "Isonitrosoacetophenone Drives Transcriptional Reprogramming", "ethylene pathways", "pathogen Pseudomonas syringae pv", "transcription activation"], "article_id"=>1302537, "categories"=>["Uncategorised"], "users"=>["Arnaud T. Djami-Tchatchou", "Mmapula P. Maake", "Lizelle A. Piater", "Ian A. Dubery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117377.g001", "stats"=>{"downloads"=>2, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Classification_of_INAP_induced_transcripts_according_to_functional_categories_/1302537", "title"=>"Classification of INAP-induced transcripts according to functional categories.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 03:44:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/1883490", "https://ndownloader.figshare.com/files/1883491", "https://ndownloader.figshare.com/files/1883492", "https://ndownloader.figshare.com/files/1883493", "https://ndownloader.figshare.com/files/1883494"], "description"=>"<div><p>Plants respond to various stress stimuli by activating broad-spectrum defense responses both locally as well as systemically. As such, identification of expressed genes represents an important step towards understanding inducible defense responses and assists in designing appropriate intervention strategies for disease management. Genes differentially expressed in tobacco cell suspensions following elicitation with isonitrosoacetophenone (INAP) were identified using mRNA differential display and pyro-sequencing. Sequencing data produced 14579 reads, which resulted in 198 contigs and 1758 singletons. Following BLAST analyses, several inducible plant defense genes of interest were identified and classified into functional categories including signal transduction, transcription activation, transcription and protein synthesis, protein degradation and ubiquitination, stress-responsive, defense-related, metabolism and energy, regulation, transportation, cytoskeleton and cell wall-related. Quantitative PCR was used to investigate the expression of 17 selected target genes within these categories. Results indicate that INAP has a sensitising or priming effect through activation of salicylic acid-, jasmonic acid- and ethylene pathways that result in an altered transcriptome, with the expression of genes involved in perception of pathogens and associated cellular re-programming in support of defense. Furthermore, infection assays with the pathogen <i>Pseudomonas syringae</i> pv. <i>tabaci</i> confirmed the establishment of a functional anti-microbial environment <i>in planta</i>.</p></div>", "links"=>[], "tags"=>["Nicotiana tabacum Cells", "sequencing data", "INAP", "Signal transduction", "Innate immunity", "BLAST analyses", "198 contigs", "infection assays", "inducible plant defense genes", "acid", "target genes", "1758 singletons", "expression", "stress stimuli", "genes differentially", "disease management", "Quantitative PCR", "protein synthesis", "understanding inducible defense responses", "Defense Plants", "intervention strategies", "tobacco cell suspensions", "category", "protein degradation", "Isonitrosoacetophenone Drives Transcriptional Reprogramming", "ethylene pathways", "pathogen Pseudomonas syringae pv", "transcription activation"], "article_id"=>1302544, "categories"=>["Uncategorised"], "users"=>["Arnaud T. Djami-Tchatchou", "Mmapula P. Maake", "Lizelle A. Piater", "Ian A. Dubery"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0117377.s001", "https://dx.doi.org/10.1371/journal.pone.0117377.s002", "https://dx.doi.org/10.1371/journal.pone.0117377.s003", "https://dx.doi.org/10.1371/journal.pone.0117377.s004", "https://dx.doi.org/10.1371/journal.pone.0117377.s005"], "stats"=>{"downloads"=>36, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Isonitrosoacetophenone_Drives_Transcriptional_Reprogramming_in_Nicotiana_tabacum_Cells_in_Support_of_Innate_Immunity_and_Defense/1302544", "title"=>"Isonitrosoacetophenone Drives Transcriptional Reprogramming in <i>Nicotiana tabacum</i> Cells in Support of Innate Immunity and Defense", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-02-06 03:44:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/1883488"], "description"=>"<p>Accession numbers of similar sequences are given in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117377#pone.0117377.s004\" target=\"_blank\">S3 Table</a>.</p><p>Summary of selected genes differentially expressed in tobacco cell suspensions in response to INAP treatment.</p>", "links"=>[], "tags"=>["Nicotiana tabacum Cells", "sequencing data", "INAP", "Signal transduction", "Innate immunity", "BLAST analyses", "198 contigs", "infection assays", "inducible plant defense genes", "acid", "target genes", "1758 singletons", "expression", "stress stimuli", "genes differentially", "disease management", "Quantitative PCR", "protein synthesis", "understanding inducible defense responses", "Defense Plants", "intervention strategies", "tobacco cell suspensions", "category", "protein degradation", "Isonitrosoacetophenone Drives Transcriptional Reprogramming", "ethylene pathways", "pathogen Pseudomonas syringae pv", "transcription activation"], "article_id"=>1302542, "categories"=>["Uncategorised"], "users"=>["Arnaud T. Djami-Tchatchou", "Mmapula P. Maake", "Lizelle A. Piater", "Ian A. Dubery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117377.t001", "stats"=>{"downloads"=>7, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_selected_genes_differentially_expressed_in_tobacco_cell_suspensions_in_response_to_INAP_treatment_/1302542", "title"=>"Summary of selected genes differentially expressed in tobacco cell suspensions in response to INAP treatment.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-02-06 03:44:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/1883485"], "description"=>"<p>The data was normalized using <i>Elf α</i> and <i>18S</i> to give the relative gene expression wherein error bars represent the standard error of mean. Expression analysis was performed on three biological repeats with two technical repeats of each. (a) SAR1-GTPase, (b) Avr9/Cf-9 rapidly elicited (ACRE-261), (c) NPR1, (d) Heat shock protein 90 (HSP90), (e) SGT1, (f) RAR1, (g) Cyclophilin, (h) Thioredoxin, (i) Ethylene response element binding protein (EREBP), (j) Cytochrome P450, (k) β-1,3-Glucanase, (l) Chitinase, (m) Pre-pro-cysteine proteinase, (n) Pheophorbide oxygenase A, (o) Biotic cell death-associated protein, (p) Pathogenesis-related protein-1b and (q) Pathogenesis-related protein-1a. Error bars: (a) indicates no significant differences, with P > 0.05, (ab) indicates a significant difference with P < 0.05, (b) indicates a highly significant difference with P< 0.01 and (bb) indicates a highly significant difference with P< 0.001.</p>", "links"=>[], "tags"=>["Nicotiana tabacum Cells", "sequencing data", "INAP", "Signal transduction", "Innate immunity", "BLAST analyses", "198 contigs", "infection assays", "inducible plant defense genes", "acid", "target genes", "1758 singletons", "expression", "stress stimuli", "genes differentially", "disease management", "Quantitative PCR", "protein synthesis", "understanding inducible defense responses", "Defense Plants", "intervention strategies", "tobacco cell suspensions", "category", "protein degradation", "Isonitrosoacetophenone Drives Transcriptional Reprogramming", "ethylene pathways", "pathogen Pseudomonas syringae pv", "transcription activation"], "article_id"=>1302539, "categories"=>["Uncategorised"], "users"=>["Arnaud T. Djami-Tchatchou", "Mmapula P. Maake", "Lizelle A. Piater", "Ian A. Dubery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117377.g002", "stats"=>{"downloads"=>4, "page_views"=>37, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_qPCR_analysis_of_differential_gene_expression_kinetics_in_Nicotiana_tabacum_cells_following_induction_with_INAP_/1302539", "title"=>"qPCR analysis of differential gene expression kinetics in <i>Nicotiana tabacum</i> cells following induction with INAP.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-06 03:44:07"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"0", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts