Syndecan-3 and TFPI Colocalize on the Surface of Endothelial-, Smooth Muscle-, and Cancer Cells
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{"title"=>"Syndecan-3 and TFPI colocalize on the surface of endothelial-, smooth muscle-, and cancer cells", "type"=>"journal", "authors"=>[{"first_name"=>"Mari", "last_name"=>"Tinholt", "scopus_author_id"=>"50462700200"}, {"first_name"=>"Benedicte", "last_name"=>"Stavik", "scopus_author_id"=>"26031851900"}, {"first_name"=>"William", "last_name"=>"Louch", "scopus_author_id"=>"6602414812"}, {"first_name"=>"Cathrine Rein", "last_name"=>"Carlson", "scopus_author_id"=>"7202968643"}, {"first_name"=>"Marit", "last_name"=>"Sletten", "scopus_author_id"=>"7003478521"}, {"first_name"=>"Wolfram", "last_name"=>"Ruf", "scopus_author_id"=>"7006016280"}, {"first_name"=>"Grethe", "last_name"=>"Skretting", "scopus_author_id"=>"56086694000"}, {"first_name"=>"Per Morten", "last_name"=>"Sandset", "scopus_author_id"=>"7004840224"}, {"first_name"=>"Nina", "last_name"=>"Iversen", "scopus_author_id"=>"55886505400"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"601868471", "pmid"=>"25617766", "doi"=>"10.1371/journal.pone.0117404", "scopus"=>"2-s2.0-84922041685", "issn"=>"19326203", "sgr"=>"84922041685"}, "id"=>"6281e48a-bb32-36a2-8075-9c79f5381fde", "abstract"=>"BACKGROUND: Tissue factor (TF) pathway inhibitor (TFPI) exists in two isoforms; TFPIα and TFPIβ. Both isoforms are cell surface attached mainly through glycosylphosphatidylinositol (GPI) anchors. TFPIα has also been proposed to bind other surface molecules, like glycosaminoglycans (GAGs). Cell surface TFPIβ has been shown to exert higher anticoagulant activity than TFPIα, suggesting alternative functions for TFPIα. Further characterization and search for novel TFPI binding partners is crucial to completely understand the biological functions of cell associated TFPI.\\n\\nMETHODS AND RESULTS: Potential association of TFPI to heparan sulphate (HS) proteoglycans in the syndecan family were evaluated by knock down studies and flow cytometry analysis. Cell surface colocalization was assessed by confocal microscopy, and native PAGE or immunoprecipitation followed by Western blotting was used to test for protein interaction. Heparanase was used to enzymatically degrade cell surface HS GAGs. Anticoagulant potential was evaluated using a factor Xa (FXa) activity assay. Knock down of syndecan-3 in endothelial,- smooth muscle- and breast cancer cells reduced the TFPI surface levels by 20-50%, and an association of TFPIα to syndecan-3 on the cell surface was demonstrated. Western blotting indicated that TFPIα was found in complex with syndecan-3. The TFPI bound to syndecan-3 did not inhibit the FXa generation. Removal of HS GAGs did not release TFPI antigen from the cells.\\n\\nCONCLUSIONS: We demonstrated an association between TFPIα and syndecan-3 in vascular cells and in cancer cells, which did not appear to depend on HS GAGs. No anticoagulant activity was detected for the TFPI associated with syndecan-3, which may indicate coagulation independent functions for this cell associated TFPI pool. This will, however, require further investigation.", "link"=>"http://www.mendeley.com/research/syndecan3-tfpi-colocalize-surface-endothelial-smooth-muscle-cancer-cells", "reader_count"=>9, "reader_count_by_academic_status"=>{"Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>2, "Medicine and Dentistry"=>1, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>2}}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1872299"], "description"=>"<p>Sum102, HCAEC, and HCASMC cells were analyzed for mRNA expression of syndecan 1–4 by qRT-PCR. The ΔΔCt method was used to calculate the relative expression (RQ) against the average endogenous control expression among all the cells, and the threshold was set at Ct = 40 (no amplification). Mean values + SD (n = 3 biological parallels) are presented.</p>", "links"=>[], "tags"=>["cell surface colocalization", "syndecan", "gpi", "cell surface HS GAGs", "breast cancer cells", "Cancer Cells BackgroundTissue factor", "TFPI surface levels", "flow cytometry analysis", "novel TFPI binding partners", "HS GAGs", "cell surface", "release TFPI antigen", "anticoagulant activity"], "article_id"=>1294477, "categories"=>["Biological Sciences"], "users"=>["Mari Tinholt", "Benedicte Stavik", "William Louch", "Cathrine Rein Carlson", "Marit Sletten", "Wolfram Ruf", "Grethe Skretting", "Per Morten Sandset", "Nina Iversen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117404.g001", "stats"=>{"downloads"=>6, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Syndecan_1_8211_4_mRNA_expression_in_Sum102_HCAEC_and_HCASMC_cells_/1294477", "title"=>"Syndecan 1–4 mRNA expression in Sum102, HCAEC, and HCASMC cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-01-24 03:05:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1872307"], "description"=>"<p>TFPI antigen levels (total) were measured by ELISA in supernatants from Sum102 (left) and HCAEC (right) after syndecan-3 knock down. Relative TFPI antigen levels compared to control cells are shown. Mean values + SD (n≥9 biological parallels) of two (HCAEC) and three (Sum102) individual experiments are presented.</p>", "links"=>[], "tags"=>["cell surface colocalization", "syndecan", "gpi", "cell surface HS GAGs", "breast cancer cells", "Cancer Cells BackgroundTissue factor", "TFPI surface levels", "flow cytometry analysis", "novel TFPI binding partners", "HS GAGs", "cell surface", "release TFPI antigen", "anticoagulant activity"], "article_id"=>1294485, "categories"=>["Biological Sciences"], "users"=>["Mari Tinholt", "Benedicte Stavik", "William Louch", "Cathrine Rein Carlson", "Marit Sletten", "Wolfram Ruf", "Grethe Skretting", "Per Morten Sandset", "Nina Iversen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117404.g008", "stats"=>{"downloads"=>4, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TFPI_antigen_levels_in_supernatant_from_Sum102_and_HCAEC_cells_after_syndecan_3_knock_down_/1294485", "title"=>"TFPI antigen levels in supernatant from Sum102 and HCAEC cells after syndecan-3 knock down.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-01-24 03:05:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1872306"], "description"=>"<p>Protein lysates were isolated from cells, subjected to native PAGE analysis or immunoprecipitation experiments and analysed by Western blotting. A) Native PAGE of lysates from Sum102 cells (20 μg), HCAECs (12 μg) and HCASMCs (18 μg) immunoblotted with anti-TFPIα (top) and anti-syndecan-3 (bottom) (one representative membrane out of three is shown), B) SDS-PAGE of full length recombinant TFPIα protein immunoblotted with anti-TFPIα, and C) SDS-PAGE of syndecan-3 293T control lysate immunoblotted with anti- syndecan-3. D) HCAEC (40 μg), E) SUM102 (160 μg) and F) HCASMC (40 μg) lysates were subjected to immunoprecipitation using two different syndecan-3 antibodies (n = 2 for sc-30883, n = 1 for sc-9495) or control goat IgG (n = 2). Lysates and immunoprecipitates were analysed for the presence of endogenous syndecan-3 and TFPI by immunoblotting. Recombinant TFPIα protein (upper left panel) and cell lysate (lower left panel) was used as positive control for the immunoblotting in D-F.</p>", "links"=>[], "tags"=>["cell surface colocalization", "syndecan", "gpi", "cell surface HS GAGs", "breast cancer cells", "Cancer Cells BackgroundTissue factor", "TFPI surface levels", "flow cytometry analysis", "novel TFPI binding partners", "HS GAGs", "cell surface", "release TFPI antigen", "anticoagulant activity"], "article_id"=>1294484, "categories"=>["Biological Sciences"], "users"=>["Mari Tinholt", "Benedicte Stavik", "William Louch", "Cathrine Rein Carlson", "Marit Sletten", "Wolfram Ruf", "Grethe Skretting", "Per Morten Sandset", "Nina Iversen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117404.g007", "stats"=>{"downloads"=>4, "page_views"=>113, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Identification_of_a_TFPI_syndecan_3_protein_complex_by_native_PAGE_and_immunoprecipitation_/1294484", "title"=>"Identification of a TFPI-syndecan-3 protein complex by native PAGE and immunoprecipitation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-01-24 03:05:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1872303"], "description"=>"<p>A) HCAEC, Sum102 and HCASMC cells with syndecan-3 knocked down were analysed for TFPI mRNA expression by qRT-PCR. The ΔΔCt method was used to calculate the relative TFPI expression (RQ) compared to control cells (Neg. Control siRNA). Mean values + SD (n≥6 biological parallels) of three individual experiments are presented. B) TFPI antigen levels were measured by ELISA in cell lysates from HCAEC, Sum102 and HCASMC after syndecan-3 knock down. TFPI antigen levels relative to control cells are shown. Mean values + SD (n≥6 biological parallels) of two individual experiments are presented.</p>", "links"=>[], "tags"=>["cell surface colocalization", "syndecan", "gpi", "cell surface HS GAGs", "breast cancer cells", "Cancer Cells BackgroundTissue factor", "TFPI surface levels", "flow cytometry analysis", "novel TFPI binding partners", "HS GAGs", "cell surface", "release TFPI antigen", "anticoagulant activity"], "article_id"=>1294481, "categories"=>["Biological Sciences"], "users"=>["Mari Tinholt", "Benedicte Stavik", "William Louch", "Cathrine Rein Carlson", "Marit Sletten", "Wolfram Ruf", "Grethe Skretting", "Per Morten Sandset", "Nina Iversen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117404.g004", "stats"=>{"downloads"=>3, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TFPI_mRNA_and_antigen_levels_in_syndecan_3_knocked_down_cells_/1294481", "title"=>"TFPI mRNA and antigen levels in syndecan-3 knocked down cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-01-24 03:05:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1872301"], "description"=>"<p>Cells knocked down for syndecan-3 by siRNA technology were analyzed for levels of cell surface TFPI by flow cytometry. The histogram presents median fluorescence intensity obtained after TFPI specific antibody labelling in A) Sum102 cells, B) HCAEC cells and C) HCASMC cells. Syndecan-3 knocked down cells (siSDC3); dashed line, negative control siRNA; black solid line and irrelevant control; grey solid line. One representative experiment of three individual experiments (n≥6 biological parallels) is shown for each cell type.</p>", "links"=>[], "tags"=>["cell surface colocalization", "syndecan", "gpi", "cell surface HS GAGs", "breast cancer cells", "Cancer Cells BackgroundTissue factor", "TFPI surface levels", "flow cytometry analysis", "novel TFPI binding partners", "HS GAGs", "cell surface", "release TFPI antigen", "anticoagulant activity"], "article_id"=>1294479, "categories"=>["Biological Sciences"], "users"=>["Mari Tinholt", "Benedicte Stavik", "William Louch", "Cathrine Rein Carlson", "Marit Sletten", "Wolfram Ruf", "Grethe Skretting", "Per Morten Sandset", "Nina Iversen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117404.g002", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Syndecan_3_knock_down_reduce_the_cell_surface_levels_of_TFPI_/1294479", "title"=>"Syndecan-3 knock down reduce the cell surface levels of TFPI.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-01-24 03:05:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1872302"], "description"=>"<p>A) Syndecan-3 antigen levels (the 55 kDa monomeric form) in cell lysates from HCAECs Sum102 cells, and HCASMCs by Western blotting using anti-syndecan-3 and loading control (actin). One representative membrane of two is shown. Syndecan-3 protein band intensities (after normalization against loading control) relative to control cells are indicated for each cell type. Recombinant syndecan-3 (rSDC3) served as a positive control (~110 kDa). B) Cell surface associated syndecan-3 levels analyzed by flow cytometry in HCAEC cells. The histogram presents median fluorescence intensity obtained after syndecan-3 specific antibody labelling. Syndecan-3 knocked down cells (siSDC3); dark grey shaded, negative control siRNA; medium grey shaded and irrelevant control; light grey shaded. One representative experiment of two individual experiments (n = 4 biological parallels) is shown.</p>", "links"=>[], "tags"=>["cell surface colocalization", "syndecan", "gpi", "cell surface HS GAGs", "breast cancer cells", "Cancer Cells BackgroundTissue factor", "TFPI surface levels", "flow cytometry analysis", "novel TFPI binding partners", "HS GAGs", "cell surface", "release TFPI antigen", "anticoagulant activity"], "article_id"=>1294480, "categories"=>["Biological Sciences"], "users"=>["Mari Tinholt", "Benedicte Stavik", "William Louch", "Cathrine Rein Carlson", "Marit Sletten", "Wolfram Ruf", "Grethe Skretting", "Per Morten Sandset", "Nina Iversen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117404.g003", "stats"=>{"downloads"=>6, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Syndecan_3_antigen_levels_in_syndecan_3_knocked_down_cells_/1294480", "title"=>"Syndecan-3 antigen levels in syndecan-3 knocked down cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-01-24 03:05:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1872311", "https://ndownloader.figshare.com/files/1872312", "https://ndownloader.figshare.com/files/1872313", "https://ndownloader.figshare.com/files/1872314", "https://ndownloader.figshare.com/files/1872316", "https://ndownloader.figshare.com/files/1872317", "https://ndownloader.figshare.com/files/1872318"], "description"=>"<div><p>Background</p><p>Tissue factor (TF) pathway inhibitor (TFPI) exists in two isoforms; TFPIα and TFPIβ. Both isoforms are cell surface attached mainly through glycosylphosphatidylinositol (GPI) anchors. TFPIα has also been proposed to bind other surface molecules, like glycosaminoglycans (GAGs). Cell surface TFPIβ has been shown to exert higher anticoagulant activity than TFPIα, suggesting alternative functions for TFPIα. Further characterization and search for novel TFPI binding partners is crucial to completely understand the biological functions of cell associated TFPI.</p><p>Methods and Results</p><p>Potential association of TFPI to heparan sulphate (HS) proteoglycans in the syndecan family were evaluated by knock down studies and flow cytometry analysis. Cell surface colocalization was assessed by confocal microscopy, and native PAGE or immunoprecipitation followed by Western blotting was used to test for protein interaction. Heparanase was used to enzymatically degrade cell surface HS GAGs. Anticoagulant potential was evaluated using a factor Xa (FXa) activity assay. Knock down of syndecan-3 in endothelial,- smooth muscle- and breast cancer cells reduced the TFPI surface levels by 20-50%, and an association of TFPIα to syndecan-3 on the cell surface was demonstrated. Western blotting indicated that TFPIα was found in complex with syndecan-3. The TFPI bound to syndecan-3 did not inhibit the FXa generation. Removal of HS GAGs did not release TFPI antigen from the cells.</p><p>Conclusions</p><p>We demonstrated an association between TFPIα and syndecan-3 in vascular cells and in cancer cells, which did not appear to depend on HS GAGs. No anticoagulant activity was detected for the TFPI associated with syndecan-3, which may indicate coagulation independent functions for this cell associated TFPI pool. This will, however, require further investigation.</p></div>", "links"=>[], "tags"=>["cell surface colocalization", "syndecan", "gpi", "cell surface HS GAGs", "breast cancer cells", "Cancer Cells BackgroundTissue factor", "TFPI surface levels", "flow cytometry analysis", "novel TFPI binding partners", "HS GAGs", "cell surface", "release TFPI antigen", "anticoagulant activity"], "article_id"=>1294489, "categories"=>["Biological Sciences"], "users"=>["Mari Tinholt", "Benedicte Stavik", "William Louch", "Cathrine Rein Carlson", "Marit Sletten", "Wolfram Ruf", "Grethe Skretting", "Per Morten Sandset", "Nina Iversen"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0117404.s001", "https://dx.doi.org/10.1371/journal.pone.0117404.s002", "https://dx.doi.org/10.1371/journal.pone.0117404.s003", "https://dx.doi.org/10.1371/journal.pone.0117404.s004", "https://dx.doi.org/10.1371/journal.pone.0117404.s005", "https://dx.doi.org/10.1371/journal.pone.0117404.s006", "https://dx.doi.org/10.1371/journal.pone.0117404.s007"], "stats"=>{"downloads"=>14, "page_views"=>30, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Syndecan_3_and_TFPI_Colocalize_on_the_Surface_of_Endothelial_Smooth_Muscle_and_Cancer_Cells_/1294489", "title"=>"Syndecan-3 and TFPI Colocalize on the Surface of Endothelial-, Smooth Muscle-, and Cancer Cells", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-01-24 03:05:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1872309"], "description"=>"<p>Sum102 and HCAEC cells knocked down of syndecan-3 (siSDC3) were analyzed for TF-FVIIa cell surface activity, indirectly, as FXa generation. HCAEC cells were stimulated with 10 nM PMA for 6 hours prior to analysis. Anti-TFPI was added to one experiment with HCAEC cells. Mean values + SD (n≥8 biological parallels) of three individual experiments are presented.</p>", "links"=>[], "tags"=>["cell surface colocalization", "syndecan", "gpi", "cell surface HS GAGs", "breast cancer cells", "Cancer Cells BackgroundTissue factor", "TFPI surface levels", "flow cytometry analysis", "novel TFPI binding partners", "HS GAGs", "cell surface", "release TFPI antigen", "anticoagulant activity"], "article_id"=>1294487, "categories"=>["Biological Sciences"], "users"=>["Mari Tinholt", "Benedicte Stavik", "William Louch", "Cathrine Rein Carlson", "Marit Sletten", "Wolfram Ruf", "Grethe Skretting", "Per Morten Sandset", "Nina Iversen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117404.g009", "stats"=>{"downloads"=>4, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TF_FVIIa_cell_surface_activity_in_Sum102_and_HCAEC_cells_after_syndecan_3_knock_down_/1294487", "title"=>"TF-FVIIa cell surface activity in Sum102 and HCAEC cells after syndecan-3 knock down.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-01-24 03:05:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1872305"], "description"=>"<p>Fixed cells were double stained with TFPI (green) and syndecan-3 (red) primary antibodies and Alexa Fluor secondary antibodies with 488 and 633 nm excitation wavelengths, respectively, before images were captured using confocal microscopy. Yellow colour in the overlay images demonstrate spatial overlap between TFPI and syndecan-3. Sum102 cells (top), HCAEC cells (middle) and HCASMC cells (bottom). Scale bar 50 μM (30 μM for zoomed images). One representative experiment of three individual experiments is shown for each cell type.</p>", "links"=>[], "tags"=>["cell surface colocalization", "syndecan", "gpi", "cell surface HS GAGs", "breast cancer cells", "Cancer Cells BackgroundTissue factor", "TFPI surface levels", "flow cytometry analysis", "novel TFPI binding partners", "HS GAGs", "cell surface", "release TFPI antigen", "anticoagulant activity"], "article_id"=>1294483, "categories"=>["Biological Sciences"], "users"=>["Mari Tinholt", "Benedicte Stavik", "William Louch", "Cathrine Rein Carlson", "Marit Sletten", "Wolfram Ruf", "Grethe Skretting", "Per Morten Sandset", "Nina Iversen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117404.g006", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TFPI_and_syndecan_3_colocalize_at_the_cell_surface_/1294483", "title"=>"TFPI and syndecan-3 colocalize at the cell surface.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-01-24 03:05:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/1872304"], "description"=>"<p>Syndecan-3 mRNA expression was measured by qRT-PCR in three independent stable clones with both isoforms of TFPI (α+β) knocked down (shRNA 4, 6 and 7) and two independent stable clones with only the TFPIβ isoform knocked down (shRNA7β and 9β). Results were normalized against endogenous control and relative expressions (RQ) were calculated in reference to the empty vector control cells (pSiRPG). Mean values + SD (n = 3 biological parallels) are presented.</p>", "links"=>[], "tags"=>["cell surface colocalization", "syndecan", "gpi", "cell surface HS GAGs", "breast cancer cells", "Cancer Cells BackgroundTissue factor", "TFPI surface levels", "flow cytometry analysis", "novel TFPI binding partners", "HS GAGs", "cell surface", "release TFPI antigen", "anticoagulant activity"], "article_id"=>1294482, "categories"=>["Biological Sciences"], "users"=>["Mari Tinholt", "Benedicte Stavik", "William Louch", "Cathrine Rein Carlson", "Marit Sletten", "Wolfram Ruf", "Grethe Skretting", "Per Morten Sandset", "Nina Iversen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117404.g005", "stats"=>{"downloads"=>2, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Syndecan_3_mRNA_expression_in_Sum102_TFPI_knock_down_cells_/1294482", "title"=>"Syndecan-3 mRNA expression in Sum102 TFPI knock down cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-01-24 03:05:00"}

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  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"26", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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