Proteome-Wide Lysine Acetylation in Cortical Astrocytes and Alterations That Occur during Infection with Brain Parasite Toxoplasma gondii
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{"title"=>"Proteome-wide lysine acetylation in cortical astrocytes and alterations that occur during infection with brain parasite Toxoplasma gondii", "type"=>"journal", "authors"=>[{"first_name"=>"Anne", "last_name"=>"Bouchut", "scopus_author_id"=>"6506036217"}, {"first_name"=>"Aarti R.", "last_name"=>"Chawla", "scopus_author_id"=>"55711377900"}, {"first_name"=>"Victoria", "last_name"=>"Jeffers", "scopus_author_id"=>"55173991100"}, {"first_name"=>"Andy", "last_name"=>"Hudmon", "scopus_author_id"=>"6602723133"}, {"first_name"=>"William J.", "last_name"=>"Sullivan", "scopus_author_id"=>"7102599574"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"25786129", "scopus"=>"2-s2.0-84925424684", "doi"=>"10.1371/journal.pone.0117966", "sgr"=>"84925424684", "pui"=>"603271342"}, "id"=>"3b7d6dfb-eb33-3a9c-829d-42c8369de3ec", "abstract"=>"Lysine acetylation is a reversible post-translational modification (PTM) that has been detected on thousands of proteins in nearly all cellular compartments. The role of this widespread PTM has yet to be fully elucidated, but can impact protein localization, interactions, activity, and stability. Here we present the first proteome-wide survey of lysine acetylation in cortical astrocytes, a subtype of glia that is a component of the blood-brain barrier and a key regulator of neuronal function and plasticity. We identified 529 lysine acetylation sites across 304 proteins found in multiple cellular compartments that largely function in RNA processing/transcription, metabolism, chromatin biology, and translation. Two hundred and seventy-seven of the acetylated lysines we identified on 186 proteins have not been reported previously in any other cell type. We also mapped an acetylome of astrocytes infected with the brain parasite, Toxoplasma gondii. It has been shown that infection with T. gondii modulates host cell gene expression, including several lysine acetyltransferase (KAT) and deacetylase (KDAC) genes, suggesting that the host acetylome may also be altered during infection. In the T. gondii-infected astrocytes, we identified 34 proteins exhibiting a level of acetylation >2-fold and 24 with a level of acetylation <2-fold relative to uninfected astrocytes. Our study documents the first acetylome map for cortical astrocytes, uncovers novel lysine acetylation sites, and demonstrates that T. gondii infection produces an altered acetylome.", "link"=>"http://www.mendeley.com/research/proteomewide-lysine-acetylation-cortical-astrocytes-alterations-occur-during-infection-brain-parasit", "reader_count"=>34, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Other"=>1, "Student > Master"=>4, "Student > Bachelor"=>7, "Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Other"=>1, "Student > Master"=>4, "Student > Bachelor"=>7, "Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>17, "Medicine and Dentistry"=>4, "Arts and Humanities"=>1, "Neuroscience"=>2, "Computer Science"=>2, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Neuroscience"=>{"Neuroscience"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>17}, "Computer Science"=>{"Computer Science"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>3}, "Arts and Humanities"=>{"Arts and Humanities"=>1}}, "reader_count_by_country"=>{"Poland"=>1, "South Africa"=>1, "Spain"=>1, "Indonesia"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1958013"], "description"=>"<p>Acetylated proteins (A) and residues (B) were sorted into functional groups based on GO analyses. Lysine acetylation is most prevalent on proteins involved in RNA processing and transcription, metabolism, and chromatin biology. C. The 277 novel acetyl-lysine sites identified in our study are present on proteins that encompass a wide range of cellular functions. D. Proteins detected as lysine-acetylated were grouped based on their respective cellular location.</p>", "links"=>[], "tags"=>["protein localization", "34 proteins", "529 lysine acetylation sites", "gondii modulates host cell gene expression", "304 proteins", "Toxoplasma gondii", "acetylated lysines", "kat", "ptm", "186 proteins", "rna", "lysine acetyltransferase", "astrocyte", "Cortical Astrocytes", "cell type", "uncovers novel lysine acetylation sites", "KDAC", "acetylome map", "chromatin biology", "gondii infection", "Brain Parasite Toxoplasma gondii Lysine acetylation", "host acetylome", "study documents", "brain parasite", "lysine acetylation"], "article_id"=>1340896, "categories"=>["Biological Sciences"], "users"=>["Anne Bouchut", "Aarti R. Chawla", "Victoria Jeffers", "Andy Hudmon", "William J. Sullivan Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117966.g002", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Global_features_of_the_cortical_astrocyte_acetylome_/1340896", "title"=>"Global features of the cortical astrocyte acetylome.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-18 03:52:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/1958016"], "description"=>"<p>Proteins involved in neurological disorders that are acetylated in murine astrocytes.</p>", "links"=>[], "tags"=>["protein localization", "34 proteins", "529 lysine acetylation sites", "gondii modulates host cell gene expression", "304 proteins", "Toxoplasma gondii", "acetylated lysines", "kat", "ptm", "186 proteins", "rna", "lysine acetyltransferase", "astrocyte", "Cortical Astrocytes", "cell type", "uncovers novel lysine acetylation sites", "KDAC", "acetylome map", "chromatin biology", "gondii infection", "Brain Parasite Toxoplasma gondii Lysine acetylation", "host acetylome", "study documents", "brain parasite", "lysine acetylation"], "article_id"=>1340899, "categories"=>["Biological Sciences"], "users"=>["Anne Bouchut", "Aarti R. Chawla", "Victoria Jeffers", "Andy Hudmon", "William J. Sullivan Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117966.t001", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proteins_involved_in_neurological_disorders_that_are_acetylated_in_murine_astrocytes_/1340899", "title"=>"Proteins involved in neurological disorders that are acetylated in murine astrocytes.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-03-18 03:52:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/1958015"], "description"=>"<p>A. Heat map of amino acid composition of acetylation sites in <i>Rattus norvegicus</i> astrocytes, displaying amino acids that are significantly enriched (green) or absent (red) relative to the general amino acid composition of the <i>Rattus norvegicus</i> proteome. B. Sequence motifs of astrocyte acetylation sites +/-7 amino acids from the targeted lysine residue. Motifs were compiled using all acetylated peptides or only those found in histones, non-histone proteins, or mitochondrial proteins.</p>", "links"=>[], "tags"=>["protein localization", "34 proteins", "529 lysine acetylation sites", "gondii modulates host cell gene expression", "304 proteins", "Toxoplasma gondii", "acetylated lysines", "kat", "ptm", "186 proteins", "rna", "lysine acetyltransferase", "astrocyte", "Cortical Astrocytes", "cell type", "uncovers novel lysine acetylation sites", "KDAC", "acetylome map", "chromatin biology", "gondii infection", "Brain Parasite Toxoplasma gondii Lysine acetylation", "host acetylome", "study documents", "brain parasite", "lysine acetylation"], "article_id"=>1340898, "categories"=>["Biological Sciences"], "users"=>["Anne Bouchut", "Aarti R. Chawla", "Victoria Jeffers", "Andy Hudmon", "William J. Sullivan Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117966.g004", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_acetylated_lysine_sites_/1340898", "title"=>"Analysis of acetylated lysine sites.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-18 03:52:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/1958017", "https://ndownloader.figshare.com/files/1958018", "https://ndownloader.figshare.com/files/1958019", "https://ndownloader.figshare.com/files/1958020"], "description"=>"<div><p>Lysine acetylation is a reversible post-translational modification (PTM) that has been detected on thousands of proteins in nearly all cellular compartments. The role of this widespread PTM has yet to be fully elucidated, but can impact protein localization, interactions, activity, and stability. Here we present the first proteome-wide survey of lysine acetylation in cortical astrocytes, a subtype of glia that is a component of the blood-brain barrier and a key regulator of neuronal function and plasticity. We identified 529 lysine acetylation sites across 304 proteins found in multiple cellular compartments that largely function in RNA processing/transcription, metabolism, chromatin biology, and translation. Two hundred and seventy-seven of the acetylated lysines we identified on 186 proteins have not been reported previously in any other cell type. We also mapped an acetylome of astrocytes infected with the brain parasite, <i>Toxoplasma gondii</i>. It has been shown that infection with <i>T</i>. <i>gondii</i> modulates host cell gene expression, including several lysine acetyltransferase (KAT) and deacetylase (KDAC) genes, suggesting that the host acetylome may also be altered during infection. In the <i>T</i>. <i>gondii</i>-infected astrocytes, we identified 34 proteins exhibiting a level of acetylation >2-fold and 24 with a level of acetylation <2-fold relative to uninfected astrocytes. Our study documents the first acetylome map for cortical astrocytes, uncovers novel lysine acetylation sites, and demonstrates that <i>T</i>. <i>gondii</i> infection produces an altered acetylome.</p></div>", "links"=>[], "tags"=>["protein localization", "34 proteins", "529 lysine acetylation sites", "gondii modulates host cell gene expression", "304 proteins", "Toxoplasma gondii", "acetylated lysines", "kat", "ptm", "186 proteins", "rna", "lysine acetyltransferase", "astrocyte", "Cortical Astrocytes", "cell type", "uncovers novel lysine acetylation sites", "KDAC", "acetylome map", "chromatin biology", "gondii infection", "Brain Parasite Toxoplasma gondii Lysine acetylation", "host acetylome", "study documents", "brain parasite", "lysine acetylation"], "article_id"=>1340900, "categories"=>["Biological Sciences"], "users"=>["Anne Bouchut", "Aarti R. Chawla", "Victoria Jeffers", "Andy Hudmon", "William J. Sullivan Jr."], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0117966.s001", "https://dx.doi.org/10.1371/journal.pone.0117966.s002", "https://dx.doi.org/10.1371/journal.pone.0117966.s003", "https://dx.doi.org/10.1371/journal.pone.0117966.s004"], "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Proteome_Wide_Lysine_Acetylation_in_Cortical_Astrocytes_and_Alterations_That_Occur_during_Infection_with_Brain_Parasite_Toxoplasma_gondii_/1340900", "title"=>"Proteome-Wide Lysine Acetylation in Cortical Astrocytes and Alterations That Occur during Infection with Brain Parasite <i>Toxoplasma gondii</i>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-03-18 03:52:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/1958014"], "description"=>"<p>The subcellular localization of α-tubulin acetylated at K40 (upper panels) and total α-tubulin (lower panels) through immunofluorescence of isolated cortical astrocytes (green). DAPI staining to highlight nuclei is indicated in blue. Scale bar = 20 μm.</p>", "links"=>[], "tags"=>["protein localization", "34 proteins", "529 lysine acetylation sites", "gondii modulates host cell gene expression", "304 proteins", "Toxoplasma gondii", "acetylated lysines", "kat", "ptm", "186 proteins", "rna", "lysine acetyltransferase", "astrocyte", "Cortical Astrocytes", "cell type", "uncovers novel lysine acetylation sites", "KDAC", "acetylome map", "chromatin biology", "gondii infection", "Brain Parasite Toxoplasma gondii Lysine acetylation", "host acetylome", "study documents", "brain parasite", "lysine acetylation"], "article_id"=>1340897, "categories"=>["Biological Sciences"], "users"=>["Anne Bouchut", "Aarti R. Chawla", "Victoria Jeffers", "Andy Hudmon", "William J. Sullivan Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117966.g003", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Localization_of_acetylated_tubulin_in_astrocytes_/1340897", "title"=>"Localization of acetylated tubulin in astrocytes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-18 03:52:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/1958012"], "description"=>"<p>Protein lysates generated from infected and uninfected cortical astrocytes were subjected to proteolysis to generate a peptide suspension. Immunoaffinity purification with anti-acetyl-lysine antibody enriched for acetylated peptides (acetylation mark is denoted by red dot). Identification of acetylated peptides was achieved with mass spectrometry.</p>", "links"=>[], "tags"=>["protein localization", "34 proteins", "529 lysine acetylation sites", "gondii modulates host cell gene expression", "304 proteins", "Toxoplasma gondii", "acetylated lysines", "kat", "ptm", "186 proteins", "rna", "lysine acetyltransferase", "astrocyte", "Cortical Astrocytes", "cell type", "uncovers novel lysine acetylation sites", "KDAC", "acetylome map", "chromatin biology", "gondii infection", "Brain Parasite Toxoplasma gondii Lysine acetylation", "host acetylome", "study documents", "brain parasite", "lysine acetylation"], "article_id"=>1340895, "categories"=>["Biological Sciences"], "users"=>["Anne Bouchut", "Aarti R. Chawla", "Victoria Jeffers", "Andy Hudmon", "William J. Sullivan Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0117966.g001", "stats"=>{"downloads"=>2, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Workflow_for_acetylome_analysis_of_cortical_astrocytes_/1340895", "title"=>"Workflow for acetylome analysis of cortical astrocytes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-18 03:52:25"}

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{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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