Sildenafil Ameliorates Long Term Peripheral Neuropathy in Type II Diabetic Mice
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Sildenafil ameliorates long term peripheral neuropathy in type II diabetic mice", "type"=>"journal", "authors"=>[{"first_name"=>"Lei", "last_name"=>"Wang", "scopus_author_id"=>"7409179472"}, {"first_name"=>"Michael", "last_name"=>"Chopp", "scopus_author_id"=>"35450613600"}, {"first_name"=>"Alexandra", "last_name"=>"Szalad", "scopus_author_id"=>"34873679100"}, {"first_name"=>"Longfei", "last_name"=>"Jia", "scopus_author_id"=>"36877976600"}, {"first_name"=>"Xuerong", "last_name"=>"Lu", "scopus_author_id"=>"56524805800"}, {"first_name"=>"Mei", "last_name"=>"Lu", "scopus_author_id"=>"26642898800"}, {"first_name"=>"Li", "last_name"=>"Zhang", "scopus_author_id"=>"56799046700"}, {"first_name"=>"Yi", "last_name"=>"Zhang", "scopus_author_id"=>"55985424500"}, {"first_name"=>"Ruilan", "last_name"=>"Zhang", "scopus_author_id"=>"8302776600"}, {"first_name"=>"Zheng Gang", "last_name"=>"Zhang", "scopus_author_id"=>"7402224335"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84923275772", "doi"=>"10.1371/journal.pone.0118134", "pui"=>"602337895", "pmid"=>"25689401", "scopus"=>"2-s2.0-84923275772", "issn"=>"19326203"}, "id"=>"00b883ef-db15-30ed-8d50-8c3dc3ab6844", "abstract"=>"Diabetic peripheral neuropathy is a common complication of long-standing diabetes mellitus. To mimic clinical trials in which patients with diabetes enrolled have advanced peripheral neuropathy, we investigated the effect of sildenafil, a specific inhibitor of phosphodiesterase type 5 enzyme, on long term peripheral neuropathy in middle aged male mice with type II diabetes. Treatment of diabetic mice (BKS.Cg-m+/+Leprdb/J, db/db) at age 36 weeks with sildenafil significantly increased functional blood vessels and regional blood flow in the sciatic nerve, concurrently with augmentation of intra-epidermal nerve fiber density in the skin and myelinated axons in the sciatic nerve. Functional analysis showed that the sildenafil treatment considerably improved motor and sensory conduction velocities in the sciatic nerve and peripheral thermal stimulus sensitivity compared with the saline treatment. In vitro studies showed that mouse dermal endothelial cells (MDE) cultured under high glucose levels exhibited significant down regulation of angiopoietin 1 (Ang1) expression and reduction of capillary-like tube formation, which were completely reversed by sildenafil. In addition, incubation of dorsal root ganglia (DRG) neurons with conditioned medium harvested from MDE under high glucose levels suppressed neurite outgrowth, where as conditional medium harvested from MDE treated with sildenafil under high glucose levels did not inhibit neurite outgrowth of DRG neurons. Moreover, blockage of the Ang1 receptor, Tie2, with a neutralized antibody against Tie2 abolished the beneficial effect of sildenafil on tube formation and neurite outgrowth. Collectively, our data indicate that sildenafil has a therapeutic effect on long term peripheral neuropathy of middle aged diabetic mice and that improvement of neurovascular dysfunction by sildenafil likely contributes to the amelioration of nerve function. The Ang1/Tie2 signaling pathway may play an important role in these restorative processes.", "link"=>"http://www.mendeley.com/research/sildenafil-ameliorates-long-term-peripheral-neuropathy-type-ii-diabetic-mice-3", "reader_count"=>20, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Researcher"=>3, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>2, "Student > Bachelor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Researcher"=>3, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>2, "Student > Bachelor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Agricultural and Biological Sciences"=>4, "Medicine and Dentistry"=>8, "Neuroscience"=>2, "Pharmacology, Toxicology and Pharmaceutical Science"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>8}, "Neuroscience"=>{"Neuroscience"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Unspecified"=>{"Unspecified"=>3}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>3}}, "reader_count_by_country"=>{"Japan"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84923275772"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84923275772?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84923275772&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84923275772&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84923275772", "dc:identifier"=>"SCOPUS_ID:84923275772", "eid"=>"2-s2.0-84923275772", "dc:title"=>"Sildenafil ameliorates long term peripheral neuropathy in type II diabetic mice", "dc:creator"=>"Wang L.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-02-17", "prism:coverDisplayDate"=>"17 February 2015", "prism:doi"=>"10.1371/journal.pone.0118134", "citedby-count"=>"21", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Henry Ford Hospital", "affiliation-city"=>"Detroit", "affiliation-country"=>"United States"}], "pubmed-id"=>"25689401", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0118134", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0118134", "share_count"=>1, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>1}

Counter

  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"69", "xml_views"=>"10", "html_views"=>"498"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"84", "xml_views"=>"1", "html_views"=>"646"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"127"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"115"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"82"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"56"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"45"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"38"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"87"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"56"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"112"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"78"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"24"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1908981"], "description"=>"<p>Treatment of male db/db mice with sildenafil improves neurological function measured by MCV (A), SCV (B), Plantar test (C) and Tail flick test (D). *p<0.05, **p<0.01 versus the non-diabetic mouse (dm) and #p<0.05, ##p<0.01 versus the diabetic mouse (db) treated with saline, respectively. n = 10/group.</p>", "links"=>[], "tags"=>["drg", "glucose levels", "age 36 weeks", "sciatic nerve", "Type II Diabetic Mice Diabetic", "neurite outgrowth", "dorsal root ganglia", "mde", "Ang 1 receptor", "sildenafil", "type II diabetes", "Sildenafil Ameliorates Long Term Peripheral Neuropathy", "neuropathy", "phosphodiesterase type 5 enzyme"], "article_id"=>1311016, "categories"=>["Biological Sciences"], "users"=>["Lei Wang", "Michael Chopp", "Alexandra Szalad", "Longfei Jia", "XueRong Lu", "Mei Lu", "Li Zhang", "Yi Zhang", "RuiLan Zhang", "Zheng Gang Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118134.g001", "stats"=>{"downloads"=>3, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sildenafil_improves_neurological_function_in_db_db_mice_/1311016", "title"=>"Sildenafil improves neurological function in db/db mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-17 03:21:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1908983"], "description"=>"<p>Panels A to L show FITC-dextran perfused vessels from whole mounted (A to D) and cross sections (E to H) of the sciatic nerve, and CD31 immunoreactive blood vessels at the cross section (I and L) of the sciatic nerve from a representative non-diabetic mouse (dm) treated with saline (A,E and I), dm mouse treated with sildenafil (dm+sil, B,F and J), diabetic mouse (db) treated with saline (C,G and K) and db mouse treated with sildenafil (db+sil, D,H and L). Panels M to O show quantitative data of density of FITC-dextran perfused vessels in cross section (M, n = 5/group), CD31 immunoreactive vascular perimeters (N, n = 10/group), and percentage changes of blood flow in the sciatic nerve with a reference of dm mice at 100% (O, n = 10/group). *p<0.05 and #p<0.05 versus the saline treated non-diabetic mouse (dm) and diabetic mouse (db), respectively. Bar = 100 μm.</p>", "links"=>[], "tags"=>["drg", "glucose levels", "age 36 weeks", "sciatic nerve", "Type II Diabetic Mice Diabetic", "neurite outgrowth", "dorsal root ganglia", "mde", "Ang 1 receptor", "sildenafil", "type II diabetes", "Sildenafil Ameliorates Long Term Peripheral Neuropathy", "neuropathy", "phosphodiesterase type 5 enzyme"], "article_id"=>1311018, "categories"=>["Biological Sciences"], "users"=>["Lei Wang", "Michael Chopp", "Alexandra Szalad", "Longfei Jia", "XueRong Lu", "Mei Lu", "Li Zhang", "Yi Zhang", "RuiLan Zhang", "Zheng Gang Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118134.g002", "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sildenafil_improves_neurovascular_function_in_the_sciatic_nerve_/1311018", "title"=>"Sildenafil improves neurovascular function in the sciatic nerve.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-17 03:21:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1908984"], "description"=>"<p>Panels A to D show PGP 9.5 immunoreactive <i>epidermal nerve fibers</i> (red, arrows) in the plantar skin from a representative non-diabetic mouse treated with saline (dm, A), non-diabetic mouse treated with sildenafil (dm+sil, B), diabetic mouse treated with saline (db, C) and diabetic mouse treated with sildenafil (db+sil, D). Panel E shows quantitative data. Panels F to I show semi-thin toluidine blue-stained cross sections of sciatic nerves from a representative non-diabetic mouse (F), non-diabetic mouse treated with sildenafil (G), diabetic mouse treated with saline (H), and diabetic mouse treated with sildenafil (I). Values are mean±SE. *p<0.05, **p<0.01 versus non-diabetic mouse (dm) treated with saline and #p<0.05, ##p<0.01 versus diabetic mouse (db) treated with saline. n = 10/group. Bar in D = 10 μm and Bar in I = 20 μm.</p>", "links"=>[], "tags"=>["drg", "glucose levels", "age 36 weeks", "sciatic nerve", "Type II Diabetic Mice Diabetic", "neurite outgrowth", "dorsal root ganglia", "mde", "Ang 1 receptor", "sildenafil", "type II diabetes", "Sildenafil Ameliorates Long Term Peripheral Neuropathy", "neuropathy", "phosphodiesterase type 5 enzyme"], "article_id"=>1311019, "categories"=>["Biological Sciences"], "users"=>["Lei Wang", "Michael Chopp", "Alexandra Szalad", "Longfei Jia", "XueRong Lu", "Mei Lu", "Li Zhang", "Yi Zhang", "RuiLan Zhang", "Zheng Gang Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118134.g003", "stats"=>{"downloads"=>3, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sildenafil_increases_axonal_remodeling_in_db_db_mice_/1311019", "title"=>"Sildenafil increases axonal remodeling in db/db mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-17 03:21:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1908985"], "description"=>"<p>Representative microscopic images (A to D) and quantitative data (I) show capillary-like tube formation of endothelial cells under regular glucose (RG), high glucose (HG), high glucose with sildenafil (HG+sil, 300 nM), and high glucose with sildenafil in the presence of a neutralizing antibody against Tie2 (+Tie2, 5 μg/ml). Panels E to H show NFH immunoreactive DRG neuron cultured in conditional medium derived from endothelial cells treated with regular glucose (RG), high glucose (HG), high glucose with sildenafil (HG+sil, 300 nM), and high glucose with sildenafil in the presence of a neutralizing antibody against Tie2 (+Tie2, 5 μg/ml). Panels J and K show quantitative data of neurite outgrowth from DRG neurons and the percentage of Schwann cell migration under different conditions listed above. Panel L shows ELISA data of Ang1 levels in conditional medium harvested from endothelial cells cultured with regular glucose (RG), high glucose (HG), high glucose with sildenafil (+sil, 300 nM). *p<0.05 versus regular glucose (RG), and #p<0.05 versus high glucose (HG), respectively. n = 6/group. Bar in D = 50 μm and Bar in H = 20 μm.</p>", "links"=>[], "tags"=>["drg", "glucose levels", "age 36 weeks", "sciatic nerve", "Type II Diabetic Mice Diabetic", "neurite outgrowth", "dorsal root ganglia", "mde", "Ang 1 receptor", "sildenafil", "type II diabetes", "Sildenafil Ameliorates Long Term Peripheral Neuropathy", "neuropathy", "phosphodiesterase type 5 enzyme"], "article_id"=>1311020, "categories"=>["Biological Sciences"], "users"=>["Lei Wang", "Michael Chopp", "Alexandra Szalad", "Longfei Jia", "XueRong Lu", "Mei Lu", "Li Zhang", "Yi Zhang", "RuiLan Zhang", "Zheng Gang Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118134.g004", "stats"=>{"downloads"=>5, "page_views"=>142, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sildenafil_induces_in_vitro_angiogenesis_DRG_neurite_outgrowth_and_Schwann_cell_migration_/1311020", "title"=>"Sildenafil induces in vitro angiogenesis, DRG neurite outgrowth and Schwann cell migration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-17 03:21:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1908986"], "description"=>"<p>Representative images of double immunofluorescent staining show that Ang1 immunoreactivity (A, C, D, F, red, arrows) was co-localized to CD31 positive vessels (B, C, green, arrows) and S100 positive Schwann cells (E, F, green, arrows). Western blot analysis (G) shows Ang1 levels in sciatic nerve tissue and β-actin was used as an internal control. ELISA data (H) show Ang1 levels in conditional medium harvested from Schwann cells cultured with regular glucose (RG), high glucose (HG), high glucose and sildenafil (+sil, 300 nM). *p<0.05 versus non-diabetic mouse (dm) treated with saline and regular glucose (RG). #p<0.05 versus diabetic mouse (db) treated with saline and high glucose (HG), respectively. n = 6/group. Bar = 50 μm.</p>", "links"=>[], "tags"=>["drg", "glucose levels", "age 36 weeks", "sciatic nerve", "Type II Diabetic Mice Diabetic", "neurite outgrowth", "dorsal root ganglia", "mde", "Ang 1 receptor", "sildenafil", "type II diabetes", "Sildenafil Ameliorates Long Term Peripheral Neuropathy", "neuropathy", "phosphodiesterase type 5 enzyme"], "article_id"=>1311021, "categories"=>["Biological Sciences"], "users"=>["Lei Wang", "Michael Chopp", "Alexandra Szalad", "Longfei Jia", "XueRong Lu", "Mei Lu", "Li Zhang", "Yi Zhang", "RuiLan Zhang", "Zheng Gang Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118134.g005", "stats"=>{"downloads"=>5, "page_views"=>42, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_Ang1_Tie2_signaling_pathway_mediates_the_effect_of_sildenafil_on_neurovascular_function_/1311021", "title"=>"The Ang1/Tie2 signaling pathway mediates the effect of sildenafil on neurovascular function.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-17 03:21:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1908987"], "description"=>"<p>Effect of sildenafil on body weight.</p>", "links"=>[], "tags"=>["drg", "glucose levels", "age 36 weeks", "sciatic nerve", "Type II Diabetic Mice Diabetic", "neurite outgrowth", "dorsal root ganglia", "mde", "Ang 1 receptor", "sildenafil", "type II diabetes", "Sildenafil Ameliorates Long Term Peripheral Neuropathy", "neuropathy", "phosphodiesterase type 5 enzyme"], "article_id"=>1311022, "categories"=>["Biological Sciences"], "users"=>["Lei Wang", "Michael Chopp", "Alexandra Szalad", "Longfei Jia", "XueRong Lu", "Mei Lu", "Li Zhang", "Yi Zhang", "RuiLan Zhang", "Zheng Gang Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118134.t001", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_sildenafil_on_body_weight_/1311022", "title"=>"Effect of sildenafil on body weight.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-02-17 03:21:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1908988"], "description"=>"<p>Effect of sildenafil on blood glucose.</p>", "links"=>[], "tags"=>["drg", "glucose levels", "age 36 weeks", "sciatic nerve", "Type II Diabetic Mice Diabetic", "neurite outgrowth", "dorsal root ganglia", "mde", "Ang 1 receptor", "sildenafil", "type II diabetes", "Sildenafil Ameliorates Long Term Peripheral Neuropathy", "neuropathy", "phosphodiesterase type 5 enzyme"], "article_id"=>1311023, "categories"=>["Biological Sciences"], "users"=>["Lei Wang", "Michael Chopp", "Alexandra Szalad", "Longfei Jia", "XueRong Lu", "Mei Lu", "Li Zhang", "Yi Zhang", "RuiLan Zhang", "Zheng Gang Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118134.t002", "stats"=>{"downloads"=>3, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_sildenafil_on_blood_glucose_/1311023", "title"=>"Effect of sildenafil on blood glucose.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-02-17 03:21:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1908989"], "description"=>"<p>Effect of sildenafil on AIC and Triglyceride.</p>", "links"=>[], "tags"=>["drg", "glucose levels", "age 36 weeks", "sciatic nerve", "Type II Diabetic Mice Diabetic", "neurite outgrowth", "dorsal root ganglia", "mde", "Ang 1 receptor", "sildenafil", "type II diabetes", "Sildenafil Ameliorates Long Term Peripheral Neuropathy", "neuropathy", "phosphodiesterase type 5 enzyme"], "article_id"=>1311024, "categories"=>["Biological Sciences"], "users"=>["Lei Wang", "Michael Chopp", "Alexandra Szalad", "Longfei Jia", "XueRong Lu", "Mei Lu", "Li Zhang", "Yi Zhang", "RuiLan Zhang", "Zheng Gang Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118134.t003", "stats"=>{"downloads"=>1, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_sildenafil_on_AIC_and_Triglyceride_/1311024", "title"=>"Effect of sildenafil on AIC and Triglyceride.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-02-17 03:21:09"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"52", "full-text"=>"60", "pdf"=>"23", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"26", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"50", "full-text"=>"59", "pdf"=>"21", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"25", "full-text"=>"22", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"86", "full-text"=>"80", "pdf"=>"48", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"37", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"26", "full-text"=>"35", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"19", "full-text"=>"23", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"30", "full-text"=>"30", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"30", "full-text"=>"24", "pdf"=>"24", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"31", "full-text"=>"30", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"25", "full-text"=>"25", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"28", "full-text"=>"20", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"21", "full-text"=>"27", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"30", "full-text"=>"40", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"24", "full-text"=>"27", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"18", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"22", "full-text"=>"19", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"25", "full-text"=>"28", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"24", "full-text"=>"27", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts