Genetic Etiology of Renal Agenesis: Fine Mapping of Renag1 and Identification of Kit as the Candidate Functional Gene
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Genetic etiology of renal agenesis: Fine mapping of Renag1 and identification of Kit as the candidate functional gene", "type"=>"journal", "authors"=>[{"first_name"=>"Nyssa Becker", "last_name"=>"Samanas", "scopus_author_id"=>"56003673600"}, {"first_name"=>"Tessa W.", "last_name"=>"Commers", "scopus_author_id"=>"23485157000"}, {"first_name"=>"Kirsten L.", "last_name"=>"Dennison", "scopus_author_id"=>"6604059730"}, {"first_name"=>"Quincy Eckert", "last_name"=>"Harenda", "scopus_author_id"=>"56525251100"}, {"first_name"=>"Scott G.", "last_name"=>"Kurz", "scopus_author_id"=>"36765127000"}, {"first_name"=>"Cynthia M.", "last_name"=>"Lachel", "scopus_author_id"=>"9236390100"}, {"first_name"=>"Kristen Leland", "last_name"=>"Wavrin", "scopus_author_id"=>"56525325700"}, {"first_name"=>"Michael", "last_name"=>"Bowler", "scopus_author_id"=>"56524988600"}, {"first_name"=>"Isaac J.", "last_name"=>"Nijman", "scopus_author_id"=>"6701321145"}, {"first_name"=>"Victor", "last_name"=>"Guryev", "scopus_author_id"=>"6602751913"}, {"first_name"=>"Edwin", "last_name"=>"Cuppen", "scopus_author_id"=>"6602072918"}, {"first_name"=>"Norbert", "last_name"=>"Hubner", "scopus_author_id"=>"7003749232"}, {"first_name"=>"Ruth", "last_name"=>"Sullivan", "scopus_author_id"=>"16492978600"}, {"first_name"=>"Chad M.", "last_name"=>"Vezina", "scopus_author_id"=>"7003334692"}, {"first_name"=>"James D.", "last_name"=>"Shull", "scopus_author_id"=>"7102952386"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"25693193", "sgr"=>"84923301686", "doi"=>"10.1371/journal.pone.0118147", "scopus"=>"2-s2.0-84923301686", "pui"=>"602339282", "issn"=>"19326203"}, "id"=>"542bf8a9-22f3-3274-9152-af3d379bf4dd", "abstract"=>"Congenital anomalies of the kidney and urogenital tract (CAKUT) occur in approximately 0.5% of live births and represent the most frequent cause of end-stage renal disease in neonates and children. The genetic basis of CAKUT is not well defined. To understand more fully the genetic basis of one type of CAKUT, unilateral renal agenesis (URA), we are studying inbred ACI rats, which spontaneously exhibit URA and associated urogenital anomalies at an incidence of approximately 10%. URA is inherited as an incompletely dominant trait with incomplete penetrance in crosses between ACI and Brown Norway (BN) rats and a single responsible genetic locus, designated Renag1, was previously mapped to rat chromosome 14 (RNO14). The goals of this study were to fine map Renag1, identify the causal genetic variant responsible for URA, confirm that the Renag1 variant is the sole determinant of URA in the ACI rat, and define the embryologic basis of URA in this rat model. Data presented herein localize Renag1 to a 379 kilobase (kb) interval that contains a single protein coding gene, Kit (v-kit Hardy-Zukerman 4 feline sarcoma viral oncogene homolog); identify an endogenous retrovirus-derived long terminal repeat located within Kit intron 1 as the probable causal variant; demonstrate aberrant development of the nephric duct in the anticipated number of ACI rat embryos; and demonstrate expression of Kit and Kit ligand (Kitlg) in the nephric duct. Congenic rats that harbor ACI alleles at Renag1 on the BN genetic background exhibit the same spectrum of urogenital anomalies as ACI rats, indicating that Renag1 is necessary and sufficient to elicit URA and associated urogenital anomalies. These data reveal the first genetic link between Kit and URA and illustrate the value of the ACI rat as a model for defining the mechanisms and cell types in which Kit functions during urogenital development.", "link"=>"http://www.mendeley.com/research/genetic-etiology-renal-agenesis-fine-mapping-renag1-identification-kit-candidate-functional-gene", "reader_count"=>19, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>1, "Student > Bachelor"=>3}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>1, "Student > Bachelor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>9, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>9}, "Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Denmark"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84923301686"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84923301686?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84923301686&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84923301686&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84923301686", "dc:identifier"=>"SCOPUS_ID:84923301686", "eid"=>"2-s2.0-84923301686", "dc:title"=>"Genetic etiology of renal agenesis: Fine mapping of Renag1 and identification of Kit as the candidate functional gene", "dc:creator"=>"Samanas N.B.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-02-18", "prism:coverDisplayDate"=>"18 February 2015", "prism:doi"=>"10.1371/journal.pone.0118147", "citedby-count"=>"2", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"McArdle Laboratory for Cancer Research", "affiliation-city"=>"Madison", "affiliation-country"=>"United States"}], "pubmed-id"=>"25693193", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0118147", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0118147", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"36", "xml_views"=>"6", "html_views"=>"136"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"74"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"91"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"82"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"22"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"29", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"6"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1910971"], "description"=>"<p>Urogenital anomalies were evaluated in a population of (BNxACI)F<sub>2</sub> rats (n = 4994) that was generated by intercrossing BN females to ACI males. <b>A.</b> The incidence of all grossly discernable urogenital anomalies in (BNxACI)F<sub>2</sub> rats is illustrated relative to that observed in ACI and BN rats. <b>B.</b> The incidence of unilateral renal agenesis (URA), unilateral renal hypoplasia (URH) and hydroureteronephrosis (HUN) in the (BNxACI)F<sub>2</sub> population is illustrated. <b>C.</b> The frequencies of URA, URH and HUN observed on the left (L) versus right (R) sides are illustrated. An asterisk indicates statistically significant right side bias (<i>p</i> ≤ 0.05). Two (BNxACI)F<sub>2</sub> rats exhibited bilateral HUN and were excluded from this analysis.</p>", "links"=>[], "tags"=>["bn", "urogenital anomalies", "ura", "Renag 1 variant", "ACI rat", "CAKUT", "RNO", "Kit intron 1", "inbred ACI rats", "rat chromosome 14", "nephric duct", "anomaly", "Renag 1", "Candidate Functional Gene Congenital anomalies", "protein coding gene", "ACI rat embryos", "harbor ACI alleles"], "article_id"=>1312431, "categories"=>["Biological Sciences"], "users"=>["Nyssa Becker Samanas", "Tessa W. Commers", "Kirsten L. Dennison", "Quincy Eckert Harenda", "Scott G. Kurz", "Cynthia M. Lachel", "Kristen Leland Wavrin", "Michael Bowler", "Isaäc J. Nijman", "Victor Guryev", "Edwin Cuppen", "Norbert Hubner", "Ruth Sullivan", "Chad M. Vezina", "James D. Shull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118147.g001", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_BNxACI_F_2_rats_exhibit_a_diverse_spectrum_of_urogenital_anomalies_/1312431", "title"=>"(BNxACI)F<sub>2</sub> rats exhibit a diverse spectrum of urogenital anomalies.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-18 03:50:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1910973"], "description"=>"<p><b>A.</b> Grossly normal renal development. <b>B.</b> Unilateral renal agenesis (URA). <b>C.</b> Unilateral renal hypoplasia (URH). <b>D.</b> Unilateral hydroureteronephrosis (HUN).</p>", "links"=>[], "tags"=>["bn", "urogenital anomalies", "ura", "Renag 1 variant", "ACI rat", "CAKUT", "RNO", "Kit intron 1", "inbred ACI rats", "rat chromosome 14", "nephric duct", "anomaly", "Renag 1", "Candidate Functional Gene Congenital anomalies", "protein coding gene", "ACI rat embryos", "harbor ACI alleles"], "article_id"=>1312433, "categories"=>["Biological Sciences"], "users"=>["Nyssa Becker Samanas", "Tessa W. Commers", "Kirsten L. Dennison", "Quincy Eckert Harenda", "Scott G. Kurz", "Cynthia M. Lachel", "Kristen Leland Wavrin", "Michael Bowler", "Isaäc J. Nijman", "Victor Guryev", "Edwin Cuppen", "Norbert Hubner", "Ruth Sullivan", "Chad M. Vezina", "James D. Shull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118147.g002", "stats"=>{"downloads"=>3, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_renal_anomalies_observed_in_BNxACI_F_2_rats_/1312433", "title"=>"Representative renal anomalies observed in (BNxACI)F<sub>2</sub> rats.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-18 03:50:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1910974"], "description"=>"<p>Because a single ACI allele at <i>Renag1</i> is sufficient to elicit urogenital anomalies in progeny generated by crossing ACI and BN rats, homozygosity for BN alleles at an RNO14 marker in a (BNxACI)F<sub>2</sub> rat affected by a urogenital anomaly excludes that marker from the <i>Renag1</i> locus. A total of 54 affected (BNxACI)F<sub>2</sub> rats harbored recombinations within <i>Renag1</i> as defined previously and were thereby informative with respect to further refining the location of the <i>Renag1</i> causal variant. The markers genotyped and their locations along RNO14 are illustrated relative to genome assembly Rnor_5.0. Genotypes observed in the indicated numbers of affected (BNxACI)F<sub>2</sub> rats are illustrated by the horizontal rows of boxes. Filled boxes represent those markers at which homozygosity for BN alleles was observed, thereby excluding that marker from <i>Renag1</i>. Open boxes represent markers at which homozygosity for ACI alleles or heterozygosity was observed. These data localize <i>Renag1</i> to the 2.0 Mb interval defined by <i>D14Uwm7</i> and <i>D14Uwm12</i>.</p>", "links"=>[], "tags"=>["bn", "urogenital anomalies", "ura", "Renag 1 variant", "ACI rat", "CAKUT", "RNO", "Kit intron 1", "inbred ACI rats", "rat chromosome 14", "nephric duct", "anomaly", "Renag 1", "Candidate Functional Gene Congenital anomalies", "protein coding gene", "ACI rat embryos", "harbor ACI alleles"], "article_id"=>1312434, "categories"=>["Biological Sciences"], "users"=>["Nyssa Becker Samanas", "Tessa W. Commers", "Kirsten L. Dennison", "Quincy Eckert Harenda", "Scott G. Kurz", "Cynthia M. Lachel", "Kristen Leland Wavrin", "Michael Bowler", "Isaäc J. Nijman", "Victor Guryev", "Edwin Cuppen", "Norbert Hubner", "Ruth Sullivan", "Chad M. Vezina", "James D. Shull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118147.g003", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Fine_mapping_of_Renag1_through_genotypic_evaluation_of_BNxACI_F_2_progeny_/1312434", "title"=>"Fine mapping of <i>Renag1</i> through genotypic evaluation of (BNxACI)F<sub>2</sub> progeny.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-18 03:50:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1910975"], "description"=>"<p>(BNxACI)F<sub>2</sub> rats determined to harbor recombinations within the <i>Renag1</i> locus were mated to BN rats and the resulting progeny were evaluated for urogenital anomalies. Genotypes across RNO14 were determined for those progeny that exhibited a urogenital anomaly. Homozygosity for BN alleles at a marker excludes that marker from <i>Renag1</i>. <b>A.</b> The 2.0 Mb <i>Renag1</i> region (Ensembl genome browser, genome assembly Rnor_5.0) is illustrated. <b>B.</b> Six recombinant chromosomes were capable of eliciting urogenital anomalies in progeny generated by backcrossing the (BNxACI)F<sub>2</sub> rats harboring those chromosomes to BN rats. The ACI segments of these chromosomes are illustrated as black bars, the segments of unknown genotype in which the recombinations occurred are illustrated by diagonal hatched bars, and the remaining segments of each chromosome are derived from BN rats. The identity of the rat of origin and the number of progeny exhibiting a urogenital anomaly are indicated to the left of each recombinant chromosome. The markers and genome coordinates at which genotypes were determined are indicated below the recombinant chromosomes. These data map <i>Renag1</i> to the 379 kb interval defined by markers <i>D14Uwm8</i> and <i>D14Uwm9</i>. This interval harbors a single protein coding gene, <i>Kit</i>.</p>", "links"=>[], "tags"=>["bn", "urogenital anomalies", "ura", "Renag 1 variant", "ACI rat", "CAKUT", "RNO", "Kit intron 1", "inbred ACI rats", "rat chromosome 14", "nephric duct", "anomaly", "Renag 1", "Candidate Functional Gene Congenital anomalies", "protein coding gene", "ACI rat embryos", "harbor ACI alleles"], "article_id"=>1312435, "categories"=>["Biological Sciences"], "users"=>["Nyssa Becker Samanas", "Tessa W. Commers", "Kirsten L. Dennison", "Quincy Eckert Harenda", "Scott G. Kurz", "Cynthia M. Lachel", "Kristen Leland Wavrin", "Michael Bowler", "Isaäc J. Nijman", "Victor Guryev", "Edwin Cuppen", "Norbert Hubner", "Ruth Sullivan", "Chad M. Vezina", "James D. Shull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118147.g004", "stats"=>{"downloads"=>0, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Fine_mapping_of_Renag1_through_progeny_testing_/1312435", "title"=>"Fine mapping of <i>Renag1</i> through progeny testing.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-18 03:50:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1910976"], "description"=>"<p>The Con1 and Con2 congenic rat strains were generated as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118147#sec009\" target=\"_blank\">Materials and Methods</a>. Each strain is homozygous for ACI alleles across the <i>Renag1</i> interval. <b>A.</b> The incidence of all grossly discernable urogenital anomalies in ACI (n = 216), Con1 (n = 238) and Con2 (n = 298) rats is illustrated. Numeral 1 indicates statistically significant difference relative to ACI. <b>B.</b> The frequency of unilateral renal agenesis (URA), unilateral renal hypoplasia (URH) and hydroureteronephrosis (HUN) in ACI, Con1 and Con2 rats is illustrated as a percent of total urogenital anomalies. <b>C.</b> The frequency of URA in ACI, Con1 and Con2 rats observed on the left (L) versus right (R) sides is illustrated. The asterisk indicates a statistically significant right side bias.</p>", "links"=>[], "tags"=>["bn", "urogenital anomalies", "ura", "Renag 1 variant", "ACI rat", "CAKUT", "RNO", "Kit intron 1", "inbred ACI rats", "rat chromosome 14", "nephric duct", "anomaly", "Renag 1", "Candidate Functional Gene Congenital anomalies", "protein coding gene", "ACI rat embryos", "harbor ACI alleles"], "article_id"=>1312436, "categories"=>["Biological Sciences"], "users"=>["Nyssa Becker Samanas", "Tessa W. Commers", "Kirsten L. Dennison", "Quincy Eckert Harenda", "Scott G. Kurz", "Cynthia M. Lachel", "Kristen Leland Wavrin", "Michael Bowler", "Isaäc J. Nijman", "Victor Guryev", "Edwin Cuppen", "Norbert Hubner", "Ruth Sullivan", "Chad M. Vezina", "James D. Shull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118147.g005", "stats"=>{"downloads"=>2, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_BN_ACI_Renag1_congenic_rats_exhibit_the_same_spectrum_and_incidence_of_urogenital_anomalies_as_ACI_rats_/1312436", "title"=>"BN.ACI-Renag1 congenic rats exhibit the same spectrum and incidence of urogenital anomalies as ACI rats.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-18 03:50:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1910978"], "description"=>"<p>The ventral aspects of representative ACI, BN, Con1 and Con2 rats are illustrated. ACI rats harbor the <i>Irish</i> allele at the <i>Hooded</i> locus and exhibit abdominal white spotting. BN rats harbor the <i>Self</i> allele at <i>Hooded</i> and are fully pigmented on the abdomen. Con1 and Con2 rats harbor the ACI derived <i>Irish</i> allele at <i>Hooded</i> and exhibit varying amounts of abdominal white spotting and a clear demarcation of pigmentation on the rear feet (noted by arrows).</p>", "links"=>[], "tags"=>["bn", "urogenital anomalies", "ura", "Renag 1 variant", "ACI rat", "CAKUT", "RNO", "Kit intron 1", "inbred ACI rats", "rat chromosome 14", "nephric duct", "anomaly", "Renag 1", "Candidate Functional Gene Congenital anomalies", "protein coding gene", "ACI rat embryos", "harbor ACI alleles"], "article_id"=>1312438, "categories"=>["Biological Sciences"], "users"=>["Nyssa Becker Samanas", "Tessa W. Commers", "Kirsten L. Dennison", "Quincy Eckert Harenda", "Scott G. Kurz", "Cynthia M. Lachel", "Kristen Leland Wavrin", "Michael Bowler", "Isaäc J. Nijman", "Victor Guryev", "Edwin Cuppen", "Norbert Hubner", "Ruth Sullivan", "Chad M. Vezina", "James D. Shull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118147.g006", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Abdominal_white_spotting_is_linked_to_the_Renag1_congenic_interval_/1312438", "title"=>"Abdominal white spotting is linked to the <i>Renag1</i> congenic interval.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-18 03:50:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1910982"], "description"=>"<p>The Hooded coat color phenotype exhibited by COP rats results from the insertion of an endogenous retrovirus (ERV) into intron 1 of <i>Kit</i>. The Irish phenotype characteristic of ACI rats results from the presence of a solitary ERV long terminal repeat (LTR) at the same location within <i>Kit</i> intron 1. <b>A.</b> This panel illustrates the site and antisense orientation of the ERV insertion relative to <i>Kit</i>. Also illustrated are the relative positions of the sequences that correspond to the AB and CD primer pairs used to evaluate the site and type of ERV/LTR insertion. <b>B.</b><u>Left five lanes</u>. The AB primer pair generated a 1006 bp PCR product from BN (<i>Self</i> allele at <i>Hooded</i>) genomic DNA template. The 1590 bp PCR product generated from ACI, Con1 and Con2 genome DNA is consistent with the presence of a solitary LTR within <i>Kit</i> intron 1 (<i>Irish</i> allele at <i>Hooded</i>). No product was generated when the AB primer pair was used to amplify genomic DNA from COP, due to the large size of the inserted ERV (<i>Hooded</i> allele). <u>Right five lanes</u>. The CD primer pair generated a 997 bp PCR product from COP (<i>Hooded</i> allele) genomic DNA. No product was generated from the CD primer pair upon amplification of genomic DNA from BN (<i>Self</i> allele), ACI, Con1 or Con2 (all <i>Irish</i> allele) rats, because primer D is within the inserted ERV element unique to the <i>Hooded</i> allele. <u>Center lane</u>. A 100 bp ladder (New England Biolabs) served as a marker for DNA fragment size. <b>C.</b> The illustrated nucleotide sequences flanking the ERV/LTR insertion site were confirmed by Sanger based capillary sequencing of PCR products generated using primer pair AB for BN, ACI, Con1 and Con2 rats, and primer pairs CD for COP rats. The upward arrow indicates the site of ERV insertion at nucleotide position 34,957,384 (rat genome assembly Rnor_5.0).</p>", "links"=>[], "tags"=>["bn", "urogenital anomalies", "ura", "Renag 1 variant", "ACI rat", "CAKUT", "RNO", "Kit intron 1", "inbred ACI rats", "rat chromosome 14", "nephric duct", "anomaly", "Renag 1", "Candidate Functional Gene Congenital anomalies", "protein coding gene", "ACI rat embryos", "harbor ACI alleles"], "article_id"=>1312442, "categories"=>["Biological Sciences"], "users"=>["Nyssa Becker Samanas", "Tessa W. Commers", "Kirsten L. Dennison", "Quincy Eckert Harenda", "Scott G. Kurz", "Cynthia M. Lachel", "Kristen Leland Wavrin", "Michael Bowler", "Isaäc J. Nijman", "Victor Guryev", "Edwin Cuppen", "Norbert Hubner", "Ruth Sullivan", "Chad M. Vezina", "James D. Shull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118147.g007", "stats"=>{"downloads"=>0, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genetic_characterization_of_the_Hooded_locus_in_ACI_BN_COP_Con1_and_Con2_rats_/1312442", "title"=>"Genetic characterization of the <i>Hooded</i> locus in ACI, BN, COP, Con1 and Con2 rats.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-18 03:50:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1910983"], "description"=>"<p>e11.5 ACI rat embryos were stained by ISH to visualize mRNA expression (purple) of the nephric duct epithelium marker, paired box gene 2 (<i>Pax2</i>). The embryos were stained intact and the caudal portion was dissected after staining and positioned for optimal imaging. <b>A.</b> Representative images of each embryo’s caudal region revealed two different <i>Pax2</i> staining patterns: the normal pattern, where <i>Pax2</i> staining is present and extends to the cloaca in both nephric ducts, and the abnormal pattern, where <i>Pax2</i> staining in one nephric duct inappropriately terminates cranial to the hindlimb (indicated by white arrowheads). Note that in the same embryo, staining in the contralateral nephric duct extends past the caudal aspect of the hindlimb to the cloaca. <b>B.</b> Some whole-mount stained embryos were cut into transverse sections to reveal histological architecture. In most embryos, <i>Pax2</i> staining was identified in the bilaterally symmetrical nephric duct cell condensates. However, some embryos featured an atypical unilateral <i>Pax2</i> staining pattern. There was no histologically discernible cell condensate in the expected nephric duct region on the unstained side of the embryo (dashed circle). Abbreviations used are: ND, nephric duct; MT, mesonephric tubule; and NT, neural tube. Both images are the same magnification. <b>C.</b> Incidence of normal <i>Pax2</i> staining in both nephric ducts and abnormal (i.e., absent) staining in left and right nephric ducts of 75 rat embryos.</p>", "links"=>[], "tags"=>["bn", "urogenital anomalies", "ura", "Renag 1 variant", "ACI rat", "CAKUT", "RNO", "Kit intron 1", "inbred ACI rats", "rat chromosome 14", "nephric duct", "anomaly", "Renag 1", "Candidate Functional Gene Congenital anomalies", "protein coding gene", "ACI rat embryos", "harbor ACI alleles"], "article_id"=>1312443, "categories"=>["Biological Sciences"], "users"=>["Nyssa Becker Samanas", "Tessa W. Commers", "Kirsten L. Dennison", "Quincy Eckert Harenda", "Scott G. Kurz", "Cynthia M. Lachel", "Kristen Leland Wavrin", "Michael Bowler", "Isaäc J. Nijman", "Victor Guryev", "Edwin Cuppen", "Norbert Hubner", "Ruth Sullivan", "Chad M. Vezina", "James D. Shull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118147.g008", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Embryologic_basis_of_urogenital_anomalies_in_ACI_rats_/1312443", "title"=>"Embryologic basis of urogenital anomalies in ACI rats.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-18 03:50:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1910985"], "description"=>"<p>e11.5 ACI rat embryos were stained by ISH to visualize mRNA expression (purple) of the nephric duct epithelium marker <i>Pax2, Kit</i> (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) or <i>Kitlg</i> (kit ligand). <b>A.</b> Lateral view of representative stained embryos. <b>B.</b> Some stained embryos were embedded in methacrylate and cut into transverse sections to reveal histological architecture. Abbreviations used are: C, cloaca; G, gut endoderm; FL, forelimb bud; HL, hindlimb bud; MT, mesonephric tubule; ND, nephric duct; and NT, neural tube. Images within each panel are of the same magnification and are representative of at least seven independent embryos.</p>", "links"=>[], "tags"=>["bn", "urogenital anomalies", "ura", "Renag 1 variant", "ACI rat", "CAKUT", "RNO", "Kit intron 1", "inbred ACI rats", "rat chromosome 14", "nephric duct", "anomaly", "Renag 1", "Candidate Functional Gene Congenital anomalies", "protein coding gene", "ACI rat embryos", "harbor ACI alleles"], "article_id"=>1312445, "categories"=>["Biological Sciences"], "users"=>["Nyssa Becker Samanas", "Tessa W. Commers", "Kirsten L. Dennison", "Quincy Eckert Harenda", "Scott G. Kurz", "Cynthia M. Lachel", "Kristen Leland Wavrin", "Michael Bowler", "Isaäc J. Nijman", "Victor Guryev", "Edwin Cuppen", "Norbert Hubner", "Ruth Sullivan", "Chad M. Vezina", "James D. Shull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118147.g009", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Kit_and_Kitlg_mRNA_are_expressed_in_the_nephric_duct_of_ACI_rat_embryos_/1312445", "title"=>"Kit and Kitlg mRNA are expressed in the nephric duct of ACI rat embryos.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-18 03:50:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1910986"], "description"=>"<div><p>Congenital anomalies of the kidney and urogenital tract (CAKUT) occur in approximately 0.5% of live births and represent the most frequent cause of end-stage renal disease in neonates and children. The genetic basis of CAKUT is not well defined. To understand more fully the genetic basis of one type of CAKUT, unilateral renal agenesis (URA), we are studying inbred ACI rats, which spontaneously exhibit URA and associated urogenital anomalies at an incidence of approximately 10%. URA is inherited as an incompletely dominant trait with incomplete penetrance in crosses between ACI and Brown Norway (BN) rats and a single responsible genetic locus, designated <i>Renag1</i>, was previously mapped to rat chromosome 14 (RNO14). The goals of this study were to fine map <i>Renag1</i>, identify the causal genetic variant responsible for URA, confirm that the <i>Renag1</i> variant is the sole determinant of URA in the ACI rat, and define the embryologic basis of URA in this rat model. Data presented herein localize <i>Renag1</i> to a 379 kilobase (kb) interval that contains a single protein coding gene, <i>Kit</i> (v-kit Hardy-Zukerman 4 feline sarcoma viral oncogene homolog); identify an endogenous retrovirus-derived long terminal repeat located within <i>Kit</i> intron 1 as the probable causal variant; demonstrate aberrant development of the nephric duct in the anticipated number of ACI rat embryos; and demonstrate expression of <i>Kit</i> and Kit ligand (<i>Kitlg</i>) in the nephric duct. Congenic rats that harbor ACI alleles at <i>Renag1</i> on the BN genetic background exhibit the same spectrum of urogenital anomalies as ACI rats, indicating that <i>Renag1</i> is necessary and sufficient to elicit URA and associated urogenital anomalies. These data reveal the first genetic link between <i>Kit</i> and URA and illustrate the value of the ACI rat as a model for defining the mechanisms and cell types in which Kit functions during urogenital development.</p></div>", "links"=>[], "tags"=>["bn", "urogenital anomalies", "ura", "Renag 1 variant", "ACI rat", "CAKUT", "RNO", "Kit intron 1", "inbred ACI rats", "rat chromosome 14", "nephric duct", "anomaly", "Renag 1", "Candidate Functional Gene Congenital anomalies", "protein coding gene", "ACI rat embryos", "harbor ACI alleles"], "article_id"=>1312446, "categories"=>["Biological Sciences"], "users"=>["Nyssa Becker Samanas", "Tessa W. Commers", "Kirsten L. Dennison", "Quincy Eckert Harenda", "Scott G. Kurz", "Cynthia M. Lachel", "Kristen Leland Wavrin", "Michael Bowler", "Isaäc J. Nijman", "Victor Guryev", "Edwin Cuppen", "Norbert Hubner", "Ruth Sullivan", "Chad M. Vezina", "James D. Shull"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118147", "stats"=>{"downloads"=>3, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Genetic_Etiology_of_Renal_Agenesis_Fine_Mapping_of_Renag1_and_Identification_of_Kit_as_the_Candidate_Functional_Gene/1312446", "title"=>"Genetic Etiology of Renal Agenesis: Fine Mapping of <i>Renag1</i> and Identification of <i>Kit</i> as the Candidate Functional Gene", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-02-18 03:50:01"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"37", "full-text"=>"42", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"23", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"30", "full-text"=>"34", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"21", "full-text"=>"23", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"31", "full-text"=>"31", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"38", "full-text"=>"33", "pdf"=>"21", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"24", "full-text"=>"26", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"24", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"23", "full-text"=>"19", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"22", "full-text"=>"13", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"18", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"25", "full-text"=>"22", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"24", "full-text"=>"22", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"30", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2020", "month"=>"9"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts