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{"title"=>"In vivo screening for anti-osteoporotic fraction from extract of herbal formula xianlinggubao in ovariectomized mice", "type"=>"journal", "authors"=>[{"first_name"=>"Xinluan", "last_name"=>"Wang", "scopus_author_id"=>"9845836900"}, {"first_name"=>"Yixin", "last_name"=>"He", "scopus_author_id"=>"55377723100"}, {"first_name"=>"Baosheng", "last_name"=>"Guo", "scopus_author_id"=>"53877402000"}, {"first_name"=>"Man Ching", "last_name"=>"Tsang", "scopus_author_id"=>"56524088100"}, {"first_name"=>"Fengjuan", "last_name"=>"Tu", "scopus_author_id"=>"24400209300"}, {"first_name"=>"Yi", "last_name"=>"Dai", "scopus_author_id"=>"23049609600"}, {"first_name"=>"Zhihong", "last_name"=>"Yao", "scopus_author_id"=>"36095209700"}, {"first_name"=>"Lizhen", "last_name"=>"Zheng", "scopus_author_id"=>"55262234600"}, {"first_name"=>"Xinhui", "last_name"=>"Xie", "scopus_author_id"=>"21935476900"}, {"first_name"=>"Nan", "last_name"=>"Wang", "scopus_author_id"=>"57133898400"}, {"first_name"=>"Xinsheng", "last_name"=>"Yao", "scopus_author_id"=>"7402530417"}, {"first_name"=>"Ge", "last_name"=>"Zhang", "scopus_author_id"=>"54394810100"}, {"first_name"=>"Ling", "last_name"=>"Qin", "scopus_author_id"=>"55722678300"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"25695519", "sgr"=>"84923250528", "doi"=>"10.1371/journal.pone.0118184", "scopus"=>"2-s2.0-84923250528", "pui"=>"602421758", "issn"=>"19326203"}, "id"=>"8093c8ae-3234-310d-b6fa-40aed753d298", "abstract"=>"BACKGROUND AND OBJECTIVES: Traditional Chinese Medicine (TCM) Fufang or formula Xianlinggubao (XLGB) is a prescribed TCM drug in China registered for prevention and treatment of osteoporosis. Fufang in TCM is comprised of a group of herbal compounds contributing in group to the treatment efficacy. The present study aims to identify the bioactive fraction(s) in XLGB extract that account(s) dominantly for its osteogenic effects.\\n\\nMETHODS: The extract of XLGB formula was separated into three fractions using chromatography, i.e., XLGB-A, XLGB-B and XLGB-C. They were administrated to 4-month old ovariectomized (OVX) mice for 6 weeks to determine which bioactive fraction(s) were more effective for preventing OVX-induced bone loss evaluated by microCT, biomechanical testing and biochemical markers. The main peaks of the key fraction were identified using reference compounds isolated from the fraction. In addition, the effects of the composite compounds in XLGB-B on osteoblasts' proliferation and mineralization were evaluated in UMR 106 cells.\\n\\nRESULTS: XLGB-B with a yield of 13.0% from herbal Fufang XLGB was identified as the most potential one among the three fractions for prevention of OVX-induced bone loss confirmed with bone mass, bone microarchitecture, bone strength and bone turnover markers. Nine compounds in HPLC fingerprint were identified in the XLGB-B fraction, including phenylpropanoids from Herba Epimedii, terpenes from Radix Dipsaci and coumarins from Fructus Psoraleae. In addition, the identified compounds effectively promoted proliferation and/or mineralization of osteoblast-like UMR 106 cells in vitro.\\n\\nCONCLUSION: XLGB-B with defined phytochemical structures was screened as the key fraction that demonstrated preventive effects on OVX-induced bone loss in mice. The present study laid down a foundation towards a new generation of herbal Fufang characterized with \"less herbal materials for achieving equal treatment efficacy\" in development strategy of TCM for prevention of OVX-induced osteoporosis.", "link"=>"http://www.mendeley.com/research/vivo-screening-antiosteoporotic-fraction-extract-herbal-formula-xianlinggubao-ovariectomized-mice", "reader_count"=>10, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>2, "Researcher"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>3, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>2, "Researcher"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>3, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>3, "Social Sciences"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Social Sciences"=>{"Social Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>3}}, "group_count"=>0}

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• http://doi.org/10.3389/fphar.2017.00273
• http://doi.org/10.1155/2017/4684962
• http://doi.org/10.1142/S0192415X16500944
• http://doi.org/10.3390/ijms19030912
• http://doi.org/10.1080/15476286.2019.1624470
• http://doi.org/10.1155/2018/1827528
• http://doi.org/10.3389/fphar.2018.01251
• http://doi.org/10.1016/j.biomaterials.2018.07.046
• http://doi.org/10.1016/j.lfs.2019.116820
• http://doi.org/10.1002/jbmr.3227
• http://doi.org/10.3109/00498254.2015.1091521
• http://doi.org/10.1155/2017/7194075
• http://doi.org/10.1142/S2575900018300023
• http://doi.org/10.3389/fphar.2017.00627
• http://doi.org/10.1016/j.nutres.2015.06.006
• http://doi.org/10.1016/j.jot.2017.11.001
• http://doi.org/10.1016/j.jep.2016.07.037
• http://doi.org/10.1111/1756-185X.13050

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Figshare

• {"files"=>["https://ndownloader.figshare.com/files/1911932"], "description"=>"<p>The white arrow pointed to the labeled the distance between two sequential fluorescent dyes in a time interval of 8 days. 20 x, bar = 10um.</p>", "links"=>[], "tags"=>["Herbal Formula Xianlinggubao", "biomechanical testing", "XLGB formula", "Herba Epimedii", "bone turnover markers", "ovx", "phytochemical structures", "bone microarchitecture", "Fufang XLGB", "6 weeks", "development strategy", "formula Xianlinggubao", "TCM drug", "bone mass", "umr", "fraction", "reference compounds", "bone strength", "Vivo Screening", "osteogenic effects.MethodsThe", "Ovariectomized Mice Background", "treatment efficacy", "HPLC fingerprint", "Fructus Psoraleae", "Radix Dipsaci", "prevention"], "article_id"=>1313205, "categories"=>["Uncategorised"], "users"=>["Xinluan Wang", "Yixin He", "Baosheng Guo", "Man-Ching Tsang", "Fengjuan Tu", "Yi Dai", "Zhihong Yao", "Lizhen Zheng", "Xinhui Xie", "Nan Wang", "Xinsheng Yao", "Ge Zhang", "Ling Qin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118184.g001", "stats"=>{"downloads"=>9, "page_views"=>50, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_fluorescent_micrographs_of_the_trabecular_bone_sections_show_the_apposition_of_the_xylenol_red_and_calcein_green_labels_in_the_sham_operated_Sham_ovariectomized_only_vehicle_treated_OVX_and_OVX_mice_treated_with_different_XLGB_fractions_/1313205", "title"=>"Representative fluorescent micrographs of the trabecular bone sections show the apposition of the xylenol (red) and calcein (green) labels in the sham-operated (Sham), ovariectomized only (vehicle-treated) (OVX), and OVX mice treated with different XLGB fractions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-19 03:01:22"}
• {"files"=>["https://ndownloader.figshare.com/files/1911933"], "description"=>"<p> The blue line is the fingerprint of UV detector and the red line is the one of ELSD. Three fractions (XLGB-A, B, C) were indicated in the square, and the XLGB-B part was amplified in the upper right square. Names of the peaks (1–9) were listed in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118184#pone.0118184.t005\" target=\"_blank\">Table 5</a>.</p>", "links"=>[], "tags"=>["Herbal Formula Xianlinggubao", "biomechanical testing", "XLGB formula", "Herba Epimedii", "bone turnover markers", "ovx", "phytochemical structures", "bone microarchitecture", "Fufang XLGB", "6 weeks", "development strategy", "formula Xianlinggubao", "TCM drug", "bone mass", "umr", "fraction", "reference compounds", "bone strength", "Vivo Screening", "osteogenic effects.MethodsThe", "Ovariectomized Mice Background", "treatment efficacy", "HPLC fingerprint", "Fructus Psoraleae", "Radix Dipsaci", "prevention"], "article_id"=>1313206, "categories"=>["Uncategorised"], "users"=>["Xinluan Wang", "Yixin He", "Baosheng Guo", "Man-Ching Tsang", "Fengjuan Tu", "Yi Dai", "Zhihong Yao", "Lizhen Zheng", "Xinhui Xie", "Nan Wang", "Xinsheng Yao", "Ge Zhang", "Ling Qin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118184.g002", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_HPLC_fingerprint_chromatograms_of_XLGB_extract_/1313206", "title"=>"HPLC fingerprint chromatograms of XLGB extract.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-19 03:01:22"}
• {"files"=>["https://ndownloader.figshare.com/files/1911934"], "description"=>"<p>Effect of the nine compounds on the mineralization of UMR 106 cells using Alizarin Red S staining for demonstrating calcium nodules.</p>", "links"=>[], "tags"=>["Herbal Formula Xianlinggubao", "biomechanical testing", "XLGB formula", "Herba Epimedii", "bone turnover markers", "ovx", "phytochemical structures", "bone microarchitecture", "Fufang XLGB", "6 weeks", "development strategy", "formula Xianlinggubao", "TCM drug", "bone mass", "umr", "fraction", "reference compounds", "bone strength", "Vivo Screening", "osteogenic effects.MethodsThe", "Ovariectomized Mice Background", "treatment efficacy", "HPLC fingerprint", "Fructus Psoraleae", "Radix Dipsaci", "prevention"], "article_id"=>1313207, "categories"=>["Uncategorised"], "users"=>["Xinluan Wang", "Yixin He", "Baosheng Guo", "Man-Ching Tsang", "Fengjuan Tu", "Yi Dai", "Zhihong Yao", "Lizhen Zheng", "Xinhui Xie", "Nan Wang", "Xinsheng Yao", "Ge Zhang", "Ling Qin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118184.g003", "stats"=>{"downloads"=>10, "page_views"=>251, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_the_nine_compounds_on_the_mineralization_of_UMR_106_cells_using_Alizarin_Red_S_staining_for_demonstrating_calcium_nodules_/1313207", "title"=>"Effect of the nine compounds on the mineralization of UMR 106 cells using Alizarin Red S staining for demonstrating calcium nodules.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-02-19 03:01:22"}
• {"files"=>["https://ndownloader.figshare.com/files/1930369"], "description"=>"<p>BMD: bone mineral density; BV/TV: bone volume/tissue volume; Tb.Th: trabecular thickness; Sham: sham-operated; OVX: ovariectomized; XLGB-L: OVX mice treated with 118 mg XLGB extract/kg body weight/day; XLGB-M: OVX mice treated with 236 mg XLGB extract/kg body weight/day; XLGB-H: OVX mice treated with 472 mg XLGB extract/kg body weight/day.</p><p>Mean value was significantly different from that of the OVX group</p><p>* P<0.05</p><p>** P<0.01</p><p>#Mice were subjected to the treatment for 6 weeks.</p><p>Effects of three doses of XLGB extracts on bone parameters as measured by microCT at the sixth lumbar vertebrae in OVX mice (mean values ± standard deviations, n = 10).</p>", "links"=>[], "tags"=>["Herbal Formula Xianlinggubao", "biomechanical testing", "XLGB formula", "Herba Epimedii", "bone turnover markers", "ovx", "phytochemical structures", "bone microarchitecture", "Fufang XLGB", "6 weeks", "development strategy", "formula Xianlinggubao", "TCM drug", "bone mass", "umr", "fraction", "reference compounds", "bone strength", "Vivo Screening", "osteogenic effects.MethodsThe", "Ovariectomized Mice Background", "treatment efficacy", "HPLC fingerprint", "Fructus Psoraleae", "Radix Dipsaci", "prevention"], "article_id"=>1322443, "categories"=>["Biological Sciences"], "users"=>["Xinluan Wang", "Yixin He", "Baosheng Guo", "Man-Ching Tsang", "Fengjuan Tu", "Yi Dai", "Zhihong Yao", "Lizhen Zheng", "Xinhui Xie", "Nan Wang", "Xinsheng Yao", "Ge Zhang", "Ling Qin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118184.t002", "stats"=>{"downloads"=>7, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_three_doses_of_XLGB_extracts_on_bone_parameters_as_measured_by_microCT_at_the_sixth_lumbar_vertebrae_in_OVX_mice_mean_values_177_standard_deviations_n_10_/1322443", "title"=>"Effects of three doses of XLGB extracts on bone parameters as measured by microCT at the sixth lumbar vertebrae in OVX mice (mean values ± standard deviations, n = 10).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-02-19 17:02:11"}
• {"files"=>["https://ndownloader.figshare.com/files/1930370"], "description"=>"<p>Sham: sham-operated; OVX: ovariectomized; XLGB-A, OVX mice treated with 140 mg XLGB-A/kg body weight/day; XLGB-B, OVX mice treated with 31 mg XLGB-B/kg body weight/day; XLGB-C, OVX mice treated with 55 mg XLGB-C/kg body weight/day; Combined XLGB: OVX mice treated with 140 mg XLGB-A, 31 mg XLGB-B and 55 mg XLGB-C kg body weight/day.</p><p>Mean value was significantly different from that of the OVX group</p><p>* P<0.05</p><p>** P<0.01</p><p>#: Mice treated for 6 weeks.</p><p>Effects of XLGB fractions on biomechanical properties, bone apposition rate and bone turnover marker in OVX mice (mean values ± standard deviations, n = 10).</p>", "links"=>[], "tags"=>["Herbal Formula Xianlinggubao", "biomechanical testing", "XLGB formula", "Herba Epimedii", "bone turnover markers", "ovx", "phytochemical structures", "bone microarchitecture", "Fufang XLGB", "6 weeks", "development strategy", "formula Xianlinggubao", "TCM drug", "bone mass", "umr", "fraction", "reference compounds", "bone strength", "Vivo Screening", "osteogenic effects.MethodsThe", "Ovariectomized Mice Background", "treatment efficacy", "HPLC fingerprint", "Fructus Psoraleae", "Radix Dipsaci", "prevention"], "article_id"=>1322444, "categories"=>["Biological Sciences"], "users"=>["Xinluan Wang", "Yixin He", "Baosheng Guo", "Man-Ching Tsang", "Fengjuan Tu", "Yi Dai", "Zhihong Yao", "Lizhen Zheng", "Xinhui Xie", "Nan Wang", "Xinsheng Yao", "Ge Zhang", "Ling Qin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118184.t004", "stats"=>{"downloads"=>6, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_XLGB_fractions_on_biomechanical_properties_bone_apposition_rate_and_bone_turnover_marker_in_OVX_mice_mean_values_177_standard_deviations_n_10_/1322444", "title"=>"Effects of XLGB fractions on biomechanical properties, bone apposition rate and bone turnover marker in OVX mice (mean values ± standard deviations, n = 10).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-02-19 17:02:11"}
• {"files"=>["https://ndownloader.figshare.com/files/1930371"], "description"=>"<p>* <b>H.E.</b>: Herba Epimedii (淫羊藿); <b>R.D.</b>: Radix Dipsaci (续断); <b>F.P.</b>: Fructus Psoraleae (补骨脂)</p><p>Rention times, names and sources of the nine compounds identified from XLGB-B fraction.</p>", "links"=>[], "tags"=>["Herbal Formula Xianlinggubao", "biomechanical testing", "XLGB formula", "Herba Epimedii", "bone turnover markers", "ovx", "phytochemical structures", "bone microarchitecture", "Fufang XLGB", "6 weeks", "development strategy", "formula Xianlinggubao", "TCM drug", "bone mass", "umr", "fraction", "reference compounds", "bone strength", "Vivo Screening", "osteogenic effects.MethodsThe", "Ovariectomized Mice Background", "treatment efficacy", "HPLC fingerprint", "Fructus Psoraleae", "Radix Dipsaci", "prevention"], "article_id"=>1322445, "categories"=>["Biological Sciences"], "users"=>["Xinluan Wang", "Yixin He", "Baosheng Guo", "Man-Ching Tsang", "Fengjuan Tu", "Yi Dai", "Zhihong Yao", "Lizhen Zheng", "Xinhui Xie", "Nan Wang", "Xinsheng Yao", "Ge Zhang", "Ling Qin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118184.t005", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Rention_times_names_and_sources_of_the_nine_compounds_identified_from_XLGB_B_fraction_/1322445", "title"=>"Rention times, names and sources of the nine compounds identified from XLGB-B fraction.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-02-19 17:02:11"}
• {"files"=>["https://ndownloader.figshare.com/files/1930372", "https://ndownloader.figshare.com/files/1930373", "https://ndownloader.figshare.com/files/1930374"], "description"=>"<div><p>Background and Objectives</p><p>Traditional Chinese Medicine (TCM) Fufang or formula Xianlinggubao (XLGB) is a prescribed TCM drug in China registered for prevention and treatment of osteoporosis. Fufang in TCM is comprised of a group of herbal compounds contributing in group to the treatment efficacy. The present study aims to identify the bioactive fraction(s) in XLGB extract that account(s) dominantly for its osteogenic effects.</p><p>Methods</p><p>The extract of XLGB formula was separated into three fractions using chromatography, i.e., XLGB-A, XLGB-B and XLGB-C. They were administrated to 4-month old ovariectomized (OVX) mice for 6 weeks to determine which bioactive fraction(s) were more effective for preventing OVX-induced bone loss evaluated by microCT, biomechanical testing and biochemical markers. The main peaks of the key fraction were identified using reference compounds isolated from the fraction. In addition, the effects of the composite compounds in XLGB-B on osteoblasts’ proliferation and mineralization were evaluated in UMR 106 cells.</p><p>Results</p><p>XLGB-B with a yield of 13.0% from herbal Fufang XLGB was identified as the most potential one among the three fractions for prevention of OVX-induced bone loss confirmed with bone mass, bone microarchitecture, bone strength and bone turnover markers. Nine compounds in HPLC fingerprint were identified in the XLGB-B fraction, including phenylpropanoids from <i>Herba Epimedii</i>, terpenes from <i>Radix Dipsaci</i> and coumarins from <i>Fructus Psoraleae</i>. In addition, the identified compounds effectively promoted proliferation and/or mineralization of osteoblast-like UMR 106 cells <i>in vitro</i>.</p><p>Conclusion</p><p>XLGB-B with defined phytochemical structures was screened as the key fraction that demonstrated preventive effects on OVX-induced bone loss in mice. The present study laid down a foundation towards a new generation of herbal Fufang characterized with “less herbal materials for achieving equal treatment efficacy” in development strategy of TCM for prevention of OVX-induced osteoporosis.</p></div>", "links"=>[], "tags"=>["Herbal Formula Xianlinggubao", "biomechanical testing", "XLGB formula", "Herba Epimedii", "bone turnover markers", "ovx", "phytochemical structures", "bone microarchitecture", "Fufang XLGB", "6 weeks", "development strategy", "formula Xianlinggubao", "TCM drug", "bone mass", "umr", "fraction", "reference compounds", "bone strength", "Vivo Screening", "osteogenic effects.MethodsThe", "Ovariectomized Mice Background", "treatment efficacy", "HPLC fingerprint", "Fructus Psoraleae", "Radix Dipsaci", "prevention"], "article_id"=>1322446, "categories"=>["Biological Sciences"], "users"=>["Xinluan Wang", "Yixin He", "Baosheng Guo", "Man-Ching Tsang", "Fengjuan Tu", "Yi Dai", "Zhihong Yao", "Lizhen Zheng", "Xinhui Xie", "Nan Wang", "Xinsheng Yao", "Ge Zhang", "Ling Qin"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0118184.s001", "https://dx.doi.org/10.1371/journal.pone.0118184.s002", "https://dx.doi.org/10.1371/journal.pone.0118184.s003"], "stats"=>{"downloads"=>3, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_Vivo_Screening_for_Anti_Osteoporotic_Fraction_from_Extract_of_Herbal_Formula_Xianlinggubao_in_Ovariectomized_Mice/1322446", "title"=>"<i>In Vivo</i> Screening for Anti-Osteoporotic Fraction from Extract of Herbal Formula Xianlinggubao in Ovariectomized Mice", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-02-19 17:02:11"}

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• {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
• {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
• {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
• {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
• {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
• {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
• {"unique-ip"=>"20", "full-text"=>"12", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}

F1000Prime