MiR-486 Regulates Lactation and Targets the PTEN Gene in Cow Mammary Glands
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{"title"=>"MiR-486 regulates lactation and targets the PTEN gene in cow mammary glands", "type"=>"journal", "authors"=>[{"first_name"=>"Dan", "last_name"=>"Li", "scopus_author_id"=>"56488913400"}, {"first_name"=>"Xuejiao", "last_name"=>"Xie", "scopus_author_id"=>"56647253600"}, {"first_name"=>"Jie", "last_name"=>"Wang", "scopus_author_id"=>"56005695100"}, {"first_name"=>"Yanjie", "last_name"=>"Bian", "scopus_author_id"=>"56115356000"}, {"first_name"=>"Qingzhang", "last_name"=>"Li", "scopus_author_id"=>"55587293800"}, {"first_name"=>"Xuejun", "last_name"=>"Gao", "scopus_author_id"=>"57145265100"}, {"first_name"=>"Chunmei", "last_name"=>"Wang", "scopus_author_id"=>"36148842800"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84929497611", "pui"=>"604166254", "doi"=>"10.1371/journal.pone.0118284", "sgr"=>"84929497611", "pmid"=>"25738494"}, "id"=>"04bfc194-9d01-375e-a5ca-0367cab5402b", "abstract"=>"Mammary gland development is controlled by several genes. Although miRNAs have been implicated in mammary gland function, the mechanism by which miR-486 regulates mammary gland development and lactation remains unclear. We investigated miR-486 expression in cow mammary gland using qRT-PCR and ISH and show that miR-486 expression was higher during the high-quality lactation period. We found that miR-486 targets phosphoinositide signaling in the cow mammary gland by directly downregulating PTEN gene expression and by altering the expression of downstream genes that are important for the function of the mammary gland, such as AKT, mTOR. We analyzed the effect of β-casein, lactose and triglyceride secretion in bovine mammary gland epithelial cells (BMECs) transfected by an inhibitor and by mimics of miR-486. Our results identify miR-486 as a downstream regulator of PTEN that is required for the development of the cow mammary gland.", "link"=>"http://www.mendeley.com/research/mir486-regulates-lactation-targets-pten-gene-cow-mammary-glands", "reader_count"=>4, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Agricultural and Biological Sciences"=>3}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1935935"], "description"=>"<p>A: ISH showing the localization of miR-486 in the mammary gland. H: lactation stage with high milk quality; L: lactation stage with low milk quality; P: pregnancy stage. (a) Mammary gland tissue incubated with a DIG-labeled locked nucleic acid probe against miR-486 (probe positive). And the white circle with solid white arrow indicates miR-486 positive probe in mammary gland tissue. (b) Mammary gland tissue incubated with a negative control probe (probe negative). And the white circle with feint arrow indicates miR-486 negative probe in mammary gland tissue. (c) Adipose tissue incubated with a DIG-labeled locked nucleic acid probe against miR-486 (fat positive). And the black circle with solid black arrow indicates miR-486 positive probe in adipose tissue. (d) Adipose tissue incubated with a negative control probe. And the black circle with feint black arrow indicates miR-486 negative probe in adipose tissue. B: The results of small RNA sequencing for miR-486 (fat negative). C: Analysis of the relative expression of miR-486 in bovine glandular tissue by qRT-PCR. n = three cows in every group. qRT-PCR reactions were performed in triplicate to detect miR-486. **<i>P</i><0.01.</p>", "links"=>[], "tags"=>["akt", "lactation period", "ish", "mir", "triglyceride secretion", "gland function", "bmec", "PTEN Gene", "Gland development", "downregulating PTEN gene expression", "Cow Mammary Glands Mammary gland development", "gland epithelial cells"], "article_id"=>1326124, "categories"=>["Biological Sciences"], "users"=>["Dan Li", "Xuejiao Xie", "Jie Wang", "Yanjie Bian", "Qingzhang Li", "Xuejun Gao", "Chunmei Wang"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0118284.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_expression_of_miR_486_in_different_milk_qualities_bovine_mammary_glands_/1326124", "title"=>"The expression of miR-486 in different milk qualities bovine mammary glands.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-04 04:10:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1935936"], "description"=>"<p>MiR-486 targets PTEN directly as measured by luciferase reporter assays, pGL3-wt: pGL3/PTEN and pGL3-mu: pGL3/PTEN/mut; pGL3-control: the empty vector, Luciferase activities of pGL3-wt are markedly decreased in cells transfected with miR-486 compared to those of reporter plasmids with mut or empty vectors. The data are the averages of at least three independent runs, and the values are the means±SD. *<i>P</i><0.05 compared to the negative control.</p>", "links"=>[], "tags"=>["akt", "lactation period", "ish", "mir", "triglyceride secretion", "gland function", "bmec", "PTEN Gene", "Gland development", "downregulating PTEN gene expression", "Cow Mammary Glands Mammary gland development", "gland epithelial cells"], "article_id"=>1326125, "categories"=>["Biological Sciences"], "users"=>["Dan Li", "Xuejiao Xie", "Jie Wang", "Yanjie Bian", "Qingzhang Li", "Xuejun Gao", "Chunmei Wang"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0118284.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_PTEN_3_8217_UTR_is_a_target_of_miR_486_/1326125", "title"=>"The PTEN 3’UTR is a target of miR-486.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-04 04:10:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1935937"], "description"=>"<p>A: qRT-PCR analysis of miR-486 expression in BMECs after transfection with miR-486 mimics or negative control (miR-NC). B: qRT-PCR analysis of miR-486 expression in BMECs after treatment with miR-486 inhibitors or the negative control (Anti-NC). C: qRT-PCR analysis of PTEN expression in BMECs after treatment with miR-486 mimics or negative control (miR-NC). D: qRT-PCR analysis of PTEN expression in BMECs after treatment with miR-486 inhibitors or negative control (Anti-NC). E: Western blotting analysis of PTEN protein expression in BMECs after treatment with miR-486 mimics or negative control (miR-NC). F: Western blotting analysis of PTEN protein expression in BMECs after treatment with miR-486 inhibitors or negative control (Anti-NC). All of the above experiments were performed in triplicate. G: Confocal microscopy analysis of PTEN expression in BMECs after treatment with miR-486 mimics (30 μM), negative control (miR-NC) (30 μM), miR-486 inhibitors (30 μM), or negative control (Anti-NC) (20 μM). (a): PTEN, (b): nuclear staining with propidium iodide, (c): merged images of (a) and (b). MiR-486 targets PTEN and represses the expression of PTEN mRNA and protein. The indicated values are the means±SD, ***<i>P</i><0.0001.</p>", "links"=>[], "tags"=>["akt", "lactation period", "ish", "mir", "triglyceride secretion", "gland function", "bmec", "PTEN Gene", "Gland development", "downregulating PTEN gene expression", "Cow Mammary Glands Mammary gland development", "gland epithelial cells"], "article_id"=>1326126, "categories"=>["Biological Sciences"], "users"=>["Dan Li", "Xuejiao Xie", "Jie Wang", "Yanjie Bian", "Qingzhang Li", "Xuejun Gao", "Chunmei Wang"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0118284.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transient_transfection_of_BMECs_with_miR_486_mimics_inhibitor_led_to_changes_in_miR_486_and_PTEN_/1326126", "title"=>"Transient transfection of BMECs with miR-486 mimics/inhibitor, led to changes in miR-486 and PTEN.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-04 04:10:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1935938"], "description"=>"<p>A: mRNA level of AKT and mTOR after treatment with miR-486 mimics and negative control (miR-NC) in BMECs; B: mRNA level of AKT and mTOR during treatment with miR-486 inhibitors and negative control (Anti-NC) in BMECs; C: Protein level of AKT, p-AKT, mTOR and p-mTOR after transfected with miR-486 mimics and negative control (miR-NC) in BMECs; D: mRNA level of AKT and mTOR during treatment with miR-486 inhibitors and negative control (Anti-NC) in BMECs. Values are means±SD, **<i>P</i><0.01, ***<i>P</i><0.0001.</p>", "links"=>[], "tags"=>["akt", "lactation period", "ish", "mir", "triglyceride secretion", "gland function", "bmec", "PTEN Gene", "Gland development", "downregulating PTEN gene expression", "Cow Mammary Glands Mammary gland development", "gland epithelial cells"], "article_id"=>1326127, "categories"=>["Biological Sciences"], "users"=>["Dan Li", "Xuejiao Xie", "Jie Wang", "Yanjie Bian", "Qingzhang Li", "Xuejun Gao", "Chunmei Wang"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0118284.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_AKT_and_mTOR_after_the_over_expression_and_inhibition_of_miR_486_in_BMECs_/1326127", "title"=>"Expression of AKT and mTOR after the over-expression and inhibition of miR-486 in BMECs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-04 04:10:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1935939"], "description"=>"<p>A: The CCK8 assay was performed at 48h after transfection of miR-486 mimics and miR-486 inhibitors; B: The percentage of EdU positive cells; C: EdU incorporation assays following transfected with miR-486 mimics, miR-486 inhibitors and their respective controls (miR-NC and Anti-NC) in BMECs for 48h. (a): all nuclear staining with Hoechst (100×), (b): nuclear staining with Edu (100×), (c): merged images of (a) and (b) (100×). Data were averages of at least three independent runs; Values are means±SD, *<i>P</i><0.05.</p>", "links"=>[], "tags"=>["akt", "lactation period", "ish", "mir", "triglyceride secretion", "gland function", "bmec", "PTEN Gene", "Gland development", "downregulating PTEN gene expression", "Cow Mammary Glands Mammary gland development", "gland epithelial cells"], "article_id"=>1326128, "categories"=>["Biological Sciences"], "users"=>["Dan Li", "Xuejiao Xie", "Jie Wang", "Yanjie Bian", "Qingzhang Li", "Xuejun Gao", "Chunmei Wang"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0118284.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effects_of_the_miR_486_mimics_and_inhibitor_on_cell_proliferation_in_BMECs_/1326128", "title"=>"The effects of the miR-486 mimics and inhibitor on cell proliferation in BMECs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-04 04:10:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1935940"], "description"=>"<p>A: With the treatment of miR-486 mimics and negative control (miR-NC) in BMECs and stained with propidium iodide for flow cytometry; B: With the treatment of miR-486 inhibitors and negative control (Anti-NC) in BMECs and stained with propidium iodide for flow cytometry. Values are means±SD, *<i>P</i><0.05.</p>", "links"=>[], "tags"=>["akt", "lactation period", "ish", "mir", "triglyceride secretion", "gland function", "bmec", "PTEN Gene", "Gland development", "downregulating PTEN gene expression", "Cow Mammary Glands Mammary gland development", "gland epithelial cells"], "article_id"=>1326129, "categories"=>["Biological Sciences"], "users"=>["Dan Li", "Xuejiao Xie", "Jie Wang", "Yanjie Bian", "Qingzhang Li", "Xuejun Gao", "Chunmei Wang"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0118284.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Changes_in_cell_cycle_distribution_in_BMECs_due_to_miR_486_mimics_and_a_miR_486_inhibitor_/1326129", "title"=>"Changes in cell cycle distribution in BMECs due to miR-486 mimics and a miR-486 inhibitor.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-04 04:10:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1935941"], "description"=>"<p>A: Secretion of triglyceride following overexpressive miR-486, compared with negative control (miR-NC); B: Secretion of triglyceride following inhibiting miR-486, compared with negative control (Anti-NC); C: Secretion of β-casein following overexpressive miR-486, compared with negative control (miR-NC); D: Secretion of β-casein following inhibiting miR-486, compared with negative control (Anti-NC); E: Secretion of lactose following overexpressive miR-486, compared with negative control (miR-NC); F: Secretion of lactose following inhibiting miR-486, compared with negative control (Anti-NC). Data were averages of at least three independent runs; Values are means±SD, <i>**P</i><0.01, <i>***P</i><0.0001.</p>", "links"=>[], "tags"=>["akt", "lactation period", "ish", "mir", "triglyceride secretion", "gland function", "bmec", "PTEN Gene", "Gland development", "downregulating PTEN gene expression", "Cow Mammary Glands Mammary gland development", "gland epithelial cells"], "article_id"=>1326130, "categories"=>["Biological Sciences"], "users"=>["Dan Li", "Xuejiao Xie", "Jie Wang", "Yanjie Bian", "Qingzhang Li", "Xuejun Gao", "Chunmei Wang"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0118284.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_secretion_in_mammary_supernatants_/1326130", "title"=>"Analysis of secretion in mammary supernatants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-04 04:10:19"}

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{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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