Potent Functional Antibody Responses Elicited by HIV-I DNA Priming and Boosting with Heterologous HIV-1 Recombinant MVA in Healthy Tanzanian Adults
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Potent functional antibody responses elicited by HIV-I DNA priming and boosting with heterologous HIV-1 recombinant MVA in healthy tanzanian adults", "type"=>"journal", "authors"=>[{"first_name"=>"Agricola", "last_name"=>"Joachim", "scopus_author_id"=>"35275206700"}, {"first_name"=>"Charlotta", "last_name"=>"Nilsson", "scopus_author_id"=>"7202947186"}, {"first_name"=>"Said", "last_name"=>"Aboud", "scopus_author_id"=>"6603530279"}, {"first_name"=>"Muhammad", "last_name"=>"Bakari", "scopus_author_id"=>"8354719300"}, {"first_name"=>"Eligius F.", "last_name"=>"Lyamuya", "scopus_author_id"=>"7004124770"}, {"first_name"=>"Merlin L.", "last_name"=>"Robb", "scopus_author_id"=>"7103268631"}, {"first_name"=>"Mary A.", "last_name"=>"Marovich", "scopus_author_id"=>"6602570248"}, {"first_name"=>"Patricia", "last_name"=>"Earl", "scopus_author_id"=>"7006720048"}, {"first_name"=>"Bernard", "last_name"=>"Moss", "scopus_author_id"=>"35417033000"}, {"first_name"=>"Christina", "last_name"=>"Ochsenbauer", "scopus_author_id"=>"7801368060"}, {"first_name"=>"Britta", "last_name"=>"Wahren", "scopus_author_id"=>"35418132000"}, {"first_name"=>"Fred", "last_name"=>"Mhalu", "scopus_author_id"=>"7005404336"}, {"first_name"=>"Eric", "last_name"=>"Sandström", "scopus_author_id"=>"7006671009"}, {"first_name"=>"Gunnel", "last_name"=>"Biberfeld", "scopus_author_id"=>"7102324699"}, {"first_name"=>"Guido", "last_name"=>"Ferrari", "scopus_author_id"=>"7201855650"}, {"first_name"=>"Victoria R.", "last_name"=>"Polonis", "scopus_author_id"=>"6603744791"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"604107789", "doi"=>"10.1371/journal.pone.0118486", "isbn"=>"2009040001075", "pmid"=>"25874723", "issn"=>"19326203", "scopus"=>"2-s2.0-84929484560", "sgr"=>"84929484560"}, "id"=>"eeff4ff7-610b-345b-bb5d-4c2cb99e1b99", "abstract"=>"UNLABELLED: Vaccine-induced HIV antibodies were evaluated in serum samples collected from healthy Tanzanian volunteers participating in a phase I/II placebo-controlled double blind trial using multi-clade, multigene HIV-DNA priming and recombinant modified vaccinia Ankara (HIV-MVA) virus boosting (HIVIS03). The HIV-DNA vaccine contained plasmids expressing HIV-1 gp160 subtypes A, B, C, Rev B, Gag A, B and RTmut B, and the recombinant HIV-MVA boost expressed CRF01_AE HIV-1 Env subtype E and Gag-Pol subtype A. While no neutralizing antibodies were detected using pseudoviruses in the TZM-bl cell assay, this prime-boost vaccination induced neutralizing antibodies in 83% of HIVIS03 vaccinees when a peripheral blood mononuclear cell (PBMC) assay using luciferase reporter-infectious molecular clones (LucR-IMC) was employed. The serum neutralizing activity was significantly (but not completely) reduced upon depletion of natural killer (NK) cells from PBMC (p=0.006), indicating a role for antibody-mediated Fcγ-receptor function. High levels of antibody-dependent cellular cytotoxicity (ADCC)-mediating antibodies against CRF01_AE and/or subtype B were subsequently demonstrated in 97% of the sera of vaccinees. The magnitude of ADCC-mediating antibodies against CM235 CRF01_AE IMC-infected cells correlated with neutralizing antibodies against CM235 in the IMC/PBMC assay. In conclusion, HIV-DNA priming, followed by two HIV-MVA boosts elicited potent ADCC responses in a high proportion of Tanzanian vaccinees. Our findings highlight the potential of HIV-DNA prime HIV-MVA boost vaccines for induction of functional antibody responses and suggest this vaccine regimen and ADCC studies as potentially important new avenues in HIV vaccine development.\\n\\nTRIAL REGISTRATION: Controlled-Trials ISRCTN90053831 The Pan African Clinical Trials Registry ATMR2009040001075080 (currently PACTR2009040001075080).", "link"=>"http://www.mendeley.com/research/potent-functional-antibody-responses-elicited-hivi-dna-priming-boosting-heterologous-hiv1-recombinan", "reader_count"=>21, "reader_count_by_academic_status"=>{"Researcher"=>3, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>2, "Student > Master"=>5, "Student > Bachelor"=>1, "Lecturer"=>1, "Professor"=>3}, "reader_count_by_user_role"=>{"Researcher"=>3, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>2, "Student > Master"=>5, "Student > Bachelor"=>1, "Lecturer"=>1, "Professor"=>3}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>3, "Nursing and Health Professions"=>1, "Medicine and Dentistry"=>6, "Agricultural and Biological Sciences"=>4, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Immunology and Microbiology"=>6}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>6}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>6}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"United Kingdom"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84929484560"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84929484560?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84929484560&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84929484560&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84929484560", "dc:identifier"=>"SCOPUS_ID:84929484560", "eid"=>"2-s2.0-84929484560", "dc:title"=>"Potent functional antibody responses elicited by HIV-I DNA priming and boosting with heterologous HIV-1 recombinant MVA in healthy tanzanian adults", "dc:creator"=>"Joachim A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-04-14", "prism:coverDisplayDate"=>"14 April 2015", "prism:doi"=>"10.1371/journal.pone.0118486", "citedby-count"=>"26", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Muhimbili University of Health and Allied Sciences", "affiliation-city"=>"Dar Es Salaam", "affiliation-country"=>"Tanzania"}, {"@_fa"=>"true", "affilname"=>"Karolinska University Hospital", "affiliation-city"=>"Stockholm", "affiliation-country"=>"Sweden"}], "pubmed-id"=>"25874723", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0118486", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0118486", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"46", "xml_views"=>"13", "html_views"=>"280"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"120"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"82"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"3"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2020179"], "description"=>"<p>ADCC-GTL: Flow cytometric antibody-dependent cellular-cytotoxicity Gran Toxi Lux-based assay</p><p>ADCC-mediating antibody response rates in 29 vaccinees primed with HIV-DNA and tested four weeks after the second HIV-MVA vaccination.</p>", "links"=>[], "tags"=>["Potent Functional Antibody Responses Elicited", "HIVIS 03 vaccinees", "PACTR", "pbmc", "crf", "neutralizing antibodies", "assay", "serum neutralizing activity", "antibody", "Env subtype E", "tanzanian", "nk", "imc", "dna", "cm", "mva", "isrctn", "gp 160 subtypes", "adcc", "Clinical Trials Registry ATMR 2009040001075080"], "article_id"=>1379213, "categories"=>["Uncategorised"], "users"=>["Agricola Joachim", "Charlotta Nilsson", "Said Aboud", "Muhammad Bakari", "Eligius F. Lyamuya", "Merlin L. Robb", "Mary A. Marovich", "Patricia Earl", "Bernard Moss", "Christina Ochsenbauer", "Britta Wahren", "Fred Mhalu", "Eric Sandstrom", "Gunnel Biberfeld", "Guido Ferrari", "Victoria R. Polonis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118486.t001", "stats"=>{"downloads"=>0, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ADCC_mediating_antibody_response_rates_in_29_vaccinees_primed_with_HIV_DNA_and_tested_four_weeks_after_the_second_HIV_MVA_vaccination_/1379213", "title"=>"ADCC-mediating antibody response rates in 29 vaccinees primed with HIV-DNA and tested four weeks after the second HIV-MVA vaccination.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-04-14 02:54:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/2020178"], "description"=>"<p>Binding antibody titers to CRF01_AE (CM243) gp120, subtype C (HIV-1<sub>96ZM651</sub>) gp140 or subtype B (IIIb) gp160 envelope protein (A). The response rate was 100% to CRF01_AE gp120 and subtype C gp140, while 26/29 (90%) vaccinees exhibited binding antibodies to subtype B gp160. There were positive correlations between: IMC/PBMC neutralizing antibody titers to CM235 and subtype C gp140 antibody binding titers (B, p = 0.02); ADCC responses to subtype CM243 CRF01_AE determined by the ADCC-GTL assay and CM243 CRF01_AE gp120 binding antibodies (C, p = 0.02).</p>", "links"=>[], "tags"=>["Potent Functional Antibody Responses Elicited", "HIVIS 03 vaccinees", "PACTR", "pbmc", "crf", "neutralizing antibodies", "assay", "serum neutralizing activity", "antibody", "Env subtype E", "tanzanian", "nk", "imc", "dna", "cm", "mva", "isrctn", "gp 160 subtypes", "adcc", "Clinical Trials Registry ATMR 2009040001075080"], "article_id"=>1379212, "categories"=>["Uncategorised"], "users"=>["Agricola Joachim", "Charlotta Nilsson", "Said Aboud", "Muhammad Bakari", "Eligius F. Lyamuya", "Merlin L. Robb", "Mary A. Marovich", "Patricia Earl", "Bernard Moss", "Christina Ochsenbauer", "Britta Wahren", "Fred Mhalu", "Eric Sandstrom", "Gunnel Biberfeld", "Guido Ferrari", "Victoria R. Polonis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118486.g006", "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Functional_and_binding_antibody_responses_in_vaccinees_tested_four_weeks_after_the_second_HIV_MVA_boost_vaccination_/1379212", "title"=>"Functional and binding antibody responses in vaccinees tested four weeks after the second HIV-MVA boost vaccination.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-14 02:54:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/2020175"], "description"=>"<p>The Spearman rank correlation between ADCC responses to SF162 subtypes B as measured by the ADCC-Luciferase assay versus the ADCC-GTL assay is shown.</p>", "links"=>[], "tags"=>["Potent Functional Antibody Responses Elicited", "HIVIS 03 vaccinees", "PACTR", "pbmc", "crf", "neutralizing antibodies", "assay", "serum neutralizing activity", "antibody", "Env subtype E", "tanzanian", "nk", "imc", "dna", "cm", "mva", "isrctn", "gp 160 subtypes", "adcc", "Clinical Trials Registry ATMR 2009040001075080"], "article_id"=>1379209, "categories"=>["Uncategorised"], "users"=>["Agricola Joachim", "Charlotta Nilsson", "Said Aboud", "Muhammad Bakari", "Eligius F. Lyamuya", "Merlin L. Robb", "Mary A. Marovich", "Patricia Earl", "Bernard Moss", "Christina Ochsenbauer", "Britta Wahren", "Fred Mhalu", "Eric Sandstrom", "Gunnel Biberfeld", "Guido Ferrari", "Victoria R. Polonis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118486.g004", "stats"=>{"downloads"=>0, "page_views"=>36, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Positive_correlation_of_ADCC_responses_between_the_ADCC_Luciferase_assay_and_the_ADCC_GTL_assay_/1379209", "title"=>"Positive correlation of ADCC responses between the ADCC-Luciferase assay and the ADCC-GTL assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-14 02:54:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/2020173"], "description"=>"<p>NK cell depletion reduces neutralizing antibody activity against CM235 at 1:20 (A) and 1:60 (B) serum dilutions, as measured using the IMC/PBMC neutralization assay. The black lines connect the neutralizing activities by sera from the same vaccinees before and after NK cell depletion.</p>", "links"=>[], "tags"=>["Potent Functional Antibody Responses Elicited", "HIVIS 03 vaccinees", "PACTR", "pbmc", "crf", "neutralizing antibodies", "assay", "serum neutralizing activity", "antibody", "Env subtype E", "tanzanian", "nk", "imc", "dna", "cm", "mva", "isrctn", "gp 160 subtypes", "adcc", "Clinical Trials Registry ATMR 2009040001075080"], "article_id"=>1379207, "categories"=>["Uncategorised"], "users"=>["Agricola Joachim", "Charlotta Nilsson", "Said Aboud", "Muhammad Bakari", "Eligius F. Lyamuya", "Merlin L. Robb", "Mary A. Marovich", "Patricia Earl", "Bernard Moss", "Christina Ochsenbauer", "Britta Wahren", "Fred Mhalu", "Eric Sandstrom", "Gunnel Biberfeld", "Guido Ferrari", "Victoria R. Polonis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118486.g002", "stats"=>{"downloads"=>0, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Impact_of_NK_cell_depletion_on_neutralizing_antibody_activity_detected_by_the_IMC_PBMC_assay_/1379207", "title"=>"Impact of NK cell depletion on neutralizing antibody activity detected by the IMC/PBMC assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-14 02:54:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/2020174"], "description"=>"<p>The magnitude of the ADCC responses against subtype B (SF162) and CRF01_AE (CM235) targets using gp120-coated targets in the ADCC-GTL assay (A) or using IMC-infected targets in the ADCC-Luciferase assay (B) is shown. In both assays, the CRF01_AE ADCC response was the most potent.</p>", "links"=>[], "tags"=>["Potent Functional Antibody Responses Elicited", "HIVIS 03 vaccinees", "PACTR", "pbmc", "crf", "neutralizing antibodies", "assay", "serum neutralizing activity", "antibody", "Env subtype E", "tanzanian", "nk", "imc", "dna", "cm", "mva", "isrctn", "gp 160 subtypes", "adcc", "Clinical Trials Registry ATMR 2009040001075080"], "article_id"=>1379208, "categories"=>["Uncategorised"], "users"=>["Agricola Joachim", "Charlotta Nilsson", "Said Aboud", "Muhammad Bakari", "Eligius F. Lyamuya", "Merlin L. Robb", "Mary A. Marovich", "Patricia Earl", "Bernard Moss", "Christina Ochsenbauer", "Britta Wahren", "Fred Mhalu", "Eric Sandstrom", "Gunnel Biberfeld", "Guido Ferrari", "Victoria R. Polonis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118486.g003", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Magnitude_of_ADCC_responses_using_IMC_infected_and_gp120_coated_targets_/1379208", "title"=>"Magnitude of ADCC responses using IMC-infected and gp120-coated targets.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-14 02:54:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/2020169"], "description"=>"<p>Neutralizing antibody responses in vaccinees four weeks after the second HIV-MVA boost vaccination as determined using the pseudovirus/TZMbl assay versus the IMC/PBMC assay (A). The neutralizing antibody activity detected by the IMC/PBMC assay was IgG-mediated (B). The solid lines indicate neutralization of the CM235 CRF01_AE IMC by unfractionated sera from two different volunteers, while the dashed lines indicate the percent neutralization by the same sera after depletion of IgG. For graphing, serum samples with no neutralizing activity at 1:20 dilutions were arbitrarily assigned a value of 10.</p>", "links"=>[], "tags"=>["Potent Functional Antibody Responses Elicited", "HIVIS 03 vaccinees", "PACTR", "pbmc", "crf", "neutralizing antibodies", "assay", "serum neutralizing activity", "antibody", "Env subtype E", "tanzanian", "nk", "imc", "dna", "cm", "mva", "isrctn", "gp 160 subtypes", "adcc", "Clinical Trials Registry ATMR 2009040001075080"], "article_id"=>1379206, "categories"=>["Uncategorised"], "users"=>["Agricola Joachim", "Charlotta Nilsson", "Said Aboud", "Muhammad Bakari", "Eligius F. Lyamuya", "Merlin L. Robb", "Mary A. Marovich", "Patricia Earl", "Bernard Moss", "Christina Ochsenbauer", "Britta Wahren", "Fred Mhalu", "Eric Sandstrom", "Gunnel Biberfeld", "Guido Ferrari", "Victoria R. Polonis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118486.g001", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Neutralizing_antibody_responses_/1379206", "title"=>"Neutralizing antibody responses.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-14 02:54:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/2020177"], "description"=>"<p>The neutralizing antibody and ADCC titers showed a positive correlation (A). The CM235 CRF01_AE titers in the ADCC-Luciferase assay were significantly higher than the CM235 CRF01_AE titers measured in the PBMC/IMC neutralizing antibody assay (B, p<0.0001).</p>", "links"=>[], "tags"=>["Potent Functional Antibody Responses Elicited", "HIVIS 03 vaccinees", "PACTR", "pbmc", "crf", "neutralizing antibodies", "assay", "serum neutralizing activity", "antibody", "Env subtype E", "tanzanian", "nk", "imc", "dna", "cm", "mva", "isrctn", "gp 160 subtypes", "adcc", "Clinical Trials Registry ATMR 2009040001075080"], "article_id"=>1379211, "categories"=>["Uncategorised"], "users"=>["Agricola Joachim", "Charlotta Nilsson", "Said Aboud", "Muhammad Bakari", "Eligius F. Lyamuya", "Merlin L. Robb", "Mary A. Marovich", "Patricia Earl", "Bernard Moss", "Christina Ochsenbauer", "Britta Wahren", "Fred Mhalu", "Eric Sandstrom", "Gunnel Biberfeld", "Guido Ferrari", "Victoria R. Polonis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118486.g005", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_neutralizing_antibody_responses_to_CM235_in_the_IMC_PBMC_assay_and_ADCC_responses_to_CM235_in_the_ADCC_Luciferase_assay_/1379211", "title"=>"Comparison of neutralizing antibody responses to CM235 in the IMC/PBMC assay and ADCC responses to CM235 in the ADCC-Luciferase assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-14 02:54:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/2020190", "https://ndownloader.figshare.com/files/2020191"], "description"=>"<div><p>Vaccine-induced HIV antibodies were evaluated in serum samples collected from healthy Tanzanian volunteers participating in a phase I/II placebo-controlled double blind trial using multi-clade, multigene HIV-DNA priming and recombinant modified vaccinia Ankara (HIV-MVA) virus boosting (HIVIS03). The HIV-DNA vaccine contained plasmids expressing HIV-1 gp160 subtypes A, B, C, Rev B, Gag A, B and RTmut B, and the recombinant HIV-MVA boost expressed CRF01_AE HIV-1 Env subtype E and Gag-Pol subtype A. While no neutralizing antibodies were detected using pseudoviruses in the TZM-bl cell assay, this prime-boost vaccination induced neutralizing antibodies in 83% of HIVIS03 vaccinees when a peripheral blood mononuclear cell (PBMC) assay using luciferase reporter-infectious molecular clones (LucR-IMC) was employed. The serum neutralizing activity was significantly (but not completely) reduced upon depletion of natural killer (NK) cells from PBMC (p=0.006), indicating a role for antibody-mediated Fcγ-receptor function. High levels of antibody-dependent cellular cytotoxicity (ADCC)-mediating antibodies against CRF01_AE and/or subtype B were subsequently demonstrated in 97% of the sera of vaccinees. The magnitude of ADCC-mediating antibodies against CM235 CRF01_AE IMC-infected cells correlated with neutralizing antibodies against CM235 in the IMC/PBMC assay. In conclusion, HIV-DNA priming, followed by two HIV-MVA boosts elicited potent ADCC responses in a high proportion of Tanzanian vaccinees. Our findings highlight the potential of HIV-DNA prime HIV-MVA boost vaccines for induction of functional antibody responses and suggest this vaccine regimen and ADCC studies as potentially important new avenues in HIV vaccine development.</p><p>Trial Registration</p><p>Controlled-Trials <a href=\"http://www.isrctn.com/ISRCTN90053831\" target=\"_blank\">ISRCTN90053831</a></p><p>The Pan African Clinical Trials Registry <a href=\"http://www.pactr.org/ATMWeb/appmanager/atm/atmregistry?dar=true&tNo=PACTR2009040001075080\" target=\"_blank\">ATMR2009040001075080 (currently PACTR2009040001075080)</a></p></div>", "links"=>[], "tags"=>["Potent Functional Antibody Responses Elicited", "HIVIS 03 vaccinees", "PACTR", "pbmc", "crf", "neutralizing antibodies", "assay", "serum neutralizing activity", "antibody", "Env subtype E", "tanzanian", "nk", "imc", "dna", "cm", "mva", "isrctn", "gp 160 subtypes", "adcc", "Clinical Trials Registry ATMR 2009040001075080"], "article_id"=>1379224, "categories"=>["Uncategorised"], "users"=>["Agricola Joachim", "Charlotta Nilsson", "Said Aboud", "Muhammad Bakari", "Eligius F. Lyamuya", "Merlin L. Robb", "Mary A. Marovich", "Patricia Earl", "Bernard Moss", "Christina Ochsenbauer", "Britta Wahren", "Fred Mhalu", "Eric Sandstrom", "Gunnel Biberfeld", "Guido Ferrari", "Victoria R. Polonis"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0118486.s001", "https://dx.doi.org/10.1371/journal.pone.0118486.s002"], "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Potent_Functional_Antibody_Responses_Elicited_by_HIV_I_DNA_Priming_and_Boosting_with_Heterologous_HIV_1_Recombinant_MVA_in_Healthy_Tanzanian_Adults_/1379224", "title"=>"Potent Functional Antibody Responses Elicited by HIV-I DNA Priming and Boosting with Heterologous HIV-1 Recombinant MVA in Healthy Tanzanian Adults", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-04-14 02:54:40"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"31", "full-text"=>"34", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"22", "full-text"=>"24", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"22", "full-text"=>"21", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"25", "full-text"=>"19", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"21", "full-text"=>"18", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"9", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"25", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"18", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts