Mouse SIRT3 Attenuates Hypertrophy-Related Lipid Accumulation in the Heart through the Deacetylation of LCAD
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Mouse SIRT3 attenuates hypertrophy-related lipid accumulation in the heart through the deacetylation of LCAD", "type"=>"journal", "authors"=>[{"first_name"=>"Tongshuai", "last_name"=>"Chen", "scopus_author_id"=>"56528580300"}, {"first_name"=>"Junni", "last_name"=>"Liu", "scopus_author_id"=>"35362213500"}, {"first_name"=>"Na", "last_name"=>"Li", "scopus_author_id"=>"56595397700"}, {"first_name"=>"Shujian", "last_name"=>"Wang", "scopus_author_id"=>"56608976200"}, {"first_name"=>"Hui", "last_name"=>"Liu", "scopus_author_id"=>"56108221800"}, {"first_name"=>"Jingyuan", "last_name"=>"Li", "scopus_author_id"=>"56609367900"}, {"first_name"=>"Yun", "last_name"=>"Zhang", "scopus_author_id"=>"56016341800"}, {"first_name"=>"Peili", "last_name"=>"Bu", "scopus_author_id"=>"35083144600"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84929121391", "sgr"=>"84929121391", "pui"=>"602763448", "doi"=>"10.1371/journal.pone.0118909"}, "id"=>"ddf164f2-8baf-3678-979f-8ef3d5a4f512", "abstract"=>"© 2015 Chen et al.Cardiac hypertrophy is an adaptive response to pressure, volume stress, and loss of contractile mass from prior infarction. Metabolic changes in cardiac hypertrophy include suppression of fatty acid oxidation and enhancement of glucose utilization, which could result in lipid accumulation in the heart. SIRT3, a mitochondrial NAD+-dependent deacetylase, has been demonstrated to play a crucial role in controlling the acetylation status of many enzymes participating in energy metabolism. However, the role of SIRT3 in the pathogenesis of hypertrophy-related lipid accumulation remains unclear. In this study, hypertrophy-related lipid accumulation was investigated using a mouse cardiac hypertrophy model induced by transverse aortic constriction (TAC). We showed that mice developed heart failure six weeks after TAC. Furthermore, abnormal lipid accumulation and decreased palmitate oxidation rates were observed in the hypertrophic hearts, and these changes were particularly significant in SIRT3-KO mice. We also demonstrated that the short form of SIRT3 was downregulated in wild-type (WT) hypertrophic hearts and that this change was accompanied by a higher acetylation level of long-chain acyl CoA dehydrogenase (LCAD), which is a key enzyme participating in fatty acid oxidation. In addition, SIRT3 may play an essential role in attenuating lipid accumulation in the heart through the deacetylation of LCAD.", "link"=>"http://www.mendeley.com/research/mouse-sirt3-attenuates-hypertrophyrelated-lipid-accumulation-heart-through-deacetylation-lcad", "reader_count"=>19, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>2, "Student > Master"=>3, "Other"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>2, "Student > Master"=>3, "Other"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>3, "Medicine and Dentistry"=>3, "Agricultural and Biological Sciences"=>7, "Physics and Astronomy"=>1, "Chemistry"=>1, "Social Sciences"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Chemistry"=>{"Chemistry"=>1}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Belgium"=>1}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84929121391"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84929121391?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84929121391&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84929121391&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84929121391", "dc:identifier"=>"SCOPUS_ID:84929121391", "eid"=>"2-s2.0-84929121391", "dc:title"=>"Mouse SIRT3 attenuates hypertrophy-related lipid accumulation in the heart through the deacetylation of LCAD", "dc:creator"=>"Chen T.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-03-06", "prism:coverDisplayDate"=>"6 March 2015", "prism:doi"=>"10.1371/journal.pone.0118909", "citedby-count"=>"46", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Qilu Hospital of Shandong University", "affiliation-city"=>"Jinan", "affiliation-country"=>"China"}], "pubmed-id"=>"25748450", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0118909", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0118909", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"79", "xml_views"=>"16", "html_views"=>"463"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"19", "xml_views"=>"2", "html_views"=>"74"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"15"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1940548"], "description"=>"<p>(A) Transmission electron micrographs of cardiac sections from SIRT3-KO mice and WT controls six weeks after sham or TAC. (×20,000). (B) Heart extracts from SIRT3-KO mice and WT controls were analyzed for triglyceride and cholesterol esters. (C) Palmitate oxidation rates in perfused hearts from WT and SIRT3-KO mice after sham or TAC. The data are presented as the means ± SEM of three independent experiments. *<i>P</i><0.05,**<i>P</i><0.01.</p>", "links"=>[], "tags"=>["nad", "acid oxidation", "wt", "attenuating lipid accumulation", "Sirt 3", "palmitate oxidation rates", "tac", "LCAD Cardiac hypertrophy", "lipid accumulation", "role", "hypertrophic hearts"], "article_id"=>1329082, "categories"=>["Biological Sciences"], "users"=>["Tongshuai Chen", "Junni Liu", "Na Li", "Shujian Wang", "Hui Liu", "Jingyuan Li", "Yun Zhang", "Peili Bu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118909.g003", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SIRT3_KO_mice_displayed_excessive_lipid_accumulation_and_decreased_palmitate_oxidation_rates_in_the_heart_/1329082", "title"=>"SIRT3-KO mice displayed excessive lipid accumulation and decreased palmitate oxidation rates in the heart.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:34:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940550"], "description"=>"<p>(A) The short form of SIRT3 (28 kDa) was localized exclusively in the mitochondria. Nuclear and mitochondrial protein extracts were immunoblotted with antibodies against the indicated proteins such as histone 3 and Hsp70. (B) The global mitochondrial acetylation level was assessed in both WT and SIRT3-KO mice subjected to sham or TAC. (C) Mitochondria extracts from SIRT3-KO mice and WT controls (sham or TAC) were immunoprecipitated with Ac-Lysine antiserum and analyzed with anti-LCAD. (D) LCAD immune complexes from hearts of SIRT3-KO mice and WT controls were immunoblotted with Ac-Lysine antibodies, and probing with LCAD antibodies revealed the total LCAD levels. (E) Expression vectors encoding FLAG-tagged murine LCAD were cotransfected with an expression vector for either Ad.SIRT3 or vehicle into cardiomyocytes. The acetylation levels for murine LCAD were measured after immunoprecipitation (anti-FLAG) by western blotting with Ac-Lysine antiserum. (F) Quantification of the acetylation level of mitochondrial proteins in WT and SIRT3-KO mice. The data are presented as the means ± SEM of three independent experiments. *<i>P</i><0.05, **<i>P</i><0.01.</p>", "links"=>[], "tags"=>["nad", "acid oxidation", "wt", "attenuating lipid accumulation", "Sirt 3", "palmitate oxidation rates", "tac", "LCAD Cardiac hypertrophy", "lipid accumulation", "role", "hypertrophic hearts"], "article_id"=>1329084, "categories"=>["Biological Sciences"], "users"=>["Tongshuai Chen", "Junni Liu", "Na Li", "Shujian Wang", "Hui Liu", "Jingyuan Li", "Yun Zhang", "Peili Bu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118909.g004", "stats"=>{"downloads"=>2, "page_views"=>78, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SIRT3_controlled_the_acetylation_status_of_LCAD_in_vivo_and_in_vitro_/1329084", "title"=>"SIRT3 controlled the acetylation status of LCAD <i>in vivo</i> and <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:34:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940546"], "description"=>"<p>(A) SIRT3 protein was absent in the hearts from SIRT3-KO mice. The protein extracts were probed with antibodies to SIRT3 (28 kDa) and β-actin. (B) SIRT3 PCR genotyping. KO, knockout; WT, wild type. (C) The ratio of the heart weight to the body weight was recorded to assess the level of hypertrophy after TAC in SIRT3-KO mice and WT controls. (D) Hematoxylin/eosin-stained cardiac sections from SIRT3-KO mice and WT controls (sham or TAC). The arrows denote areas of cardiomyocyte loss or dropout. Sections of the hearts were stained with Masson’s trichrome to detect fibrosis (blue). (E) Bar graph showing the quantification of fibrosis in the SIRT3-KO mice hearts and WT controls (sham or TAC). (F and G) The LV wall thickness and fractional shortening (FS) were measured with echocardiology as described in the methods section. The data are presented as the means ± SEM of three independent experiments. *<i>P</i><0.05, **<i>P</i><0.01.</p>", "links"=>[], "tags"=>["nad", "acid oxidation", "wt", "attenuating lipid accumulation", "Sirt 3", "palmitate oxidation rates", "tac", "LCAD Cardiac hypertrophy", "lipid accumulation", "role", "hypertrophic hearts"], "article_id"=>1329080, "categories"=>["Biological Sciences"], "users"=>["Tongshuai Chen", "Junni Liu", "Na Li", "Shujian Wang", "Hui Liu", "Jingyuan Li", "Yun Zhang", "Peili Bu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118909.g002", "stats"=>{"downloads"=>6, "page_views"=>96, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SIRT3_KO_mice_had_a_propensity_to_develop_heart_failure_in_response_to_TAC_/1329080", "title"=>"SIRT3-KO mice had a propensity to develop heart failure in response to TAC.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:34:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940544"], "description"=>"<p>(A and B) The velocity of the binding aorta was measured by echocardiology. The velocity was significantly elevated one week after TAC. (C) The degree of hypertrophy was assessed by the ratio of the heart weight to the body weight. Mice subjected to TAC produced nearly 60% cardiac hypertrophy at six weeks. (D) The subcellular fractionation from the hearts of WT mice after sham or TAC was analyzed by western blot analysis with antibodies specific for both forms of SIRT3, and β-actin was used as a reference. (E and F) Quantification of both forms of SIRT3 was determined by densitometry analysis, and β-actin was used as a reference. The densitometry value of each band was determined with the ImageJ software. The data are presented as the means ± SEM of three independent experiments. *<i>P</i><0.05, **<i>P</i><0.01.</p>", "links"=>[], "tags"=>["nad", "acid oxidation", "wt", "attenuating lipid accumulation", "Sirt 3", "palmitate oxidation rates", "tac", "LCAD Cardiac hypertrophy", "lipid accumulation", "role", "hypertrophic hearts"], "article_id"=>1329078, "categories"=>["Biological Sciences"], "users"=>["Tongshuai Chen", "Junni Liu", "Na Li", "Shujian Wang", "Hui Liu", "Jingyuan Li", "Yun Zhang", "Peili Bu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118909.g001", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_short_form_of_SIRT3_was_downregulated_in_the_murine_hypertrophic_heart_/1329078", "title"=>"The short form of SIRT3 was downregulated in the murine hypertrophic heart.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:34:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940557", "https://ndownloader.figshare.com/files/1940559", "https://ndownloader.figshare.com/files/1940560", "https://ndownloader.figshare.com/files/1940561", "https://ndownloader.figshare.com/files/1940562"], "description"=>"<div><p>Cardiac hypertrophy is an adaptive response to pressure, volume stress, and loss of contractile mass from prior infarction. Metabolic changes in cardiac hypertrophy include suppression of fatty acid oxidation and enhancement of glucose utilization, which could result in lipid accumulation in the heart. SIRT3, a mitochondrial NAD+-dependent deacetylase, has been demonstrated to play a crucial role in controlling the acetylation status of many enzymes participating in energy metabolism. However, the role of SIRT3 in the pathogenesis of hypertrophy-related lipid accumulation remains unclear. In this study, hypertrophy-related lipid accumulation was investigated using a mouse cardiac hypertrophy model induced by transverse aortic constriction (TAC). We showed that mice developed heart failure six weeks after TAC. Furthermore, abnormal lipid accumulation and decreased palmitate oxidation rates were observed in the hypertrophic hearts, and these changes were particularly significant in SIRT3-KO mice. We also demonstrated that the short form of SIRT3 was downregulated in wild-type (WT) hypertrophic hearts and that this change was accompanied by a higher acetylation level of long-chain acyl CoA dehydrogenase (LCAD), which is a key enzyme participating in fatty acid oxidation. In addition, SIRT3 may play an essential role in attenuating lipid accumulation in the heart through the deacetylation of LCAD.</p></div>", "links"=>[], "tags"=>["nad", "acid oxidation", "wt", "attenuating lipid accumulation", "Sirt 3", "palmitate oxidation rates", "tac", "LCAD Cardiac hypertrophy", "lipid accumulation", "role", "hypertrophic hearts"], "article_id"=>1329092, "categories"=>["Biological Sciences"], "users"=>["Tongshuai Chen", "Junni Liu", "Na Li", "Shujian Wang", "Hui Liu", "Jingyuan Li", "Yun Zhang", "Peili Bu"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0118909.s001", "https://dx.doi.org/10.1371/journal.pone.0118909.s002", "https://dx.doi.org/10.1371/journal.pone.0118909.s003", "https://dx.doi.org/10.1371/journal.pone.0118909.s004", "https://dx.doi.org/10.1371/journal.pone.0118909.s005"], "stats"=>{"downloads"=>7, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mouse_SIRT3_Attenuates_Hypertrophy_Related_Lipid_Accumulation_in_the_Heart_through_the_Deacetylation_of_LCAD_/1329092", "title"=>"Mouse SIRT3 Attenuates Hypertrophy-Related Lipid Accumulation in the Heart through the Deacetylation of LCAD", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-03-06 09:34:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940555"], "description"=>"<p>(A and B) Cardiomyocytes were infected with Ad.SIRT3 or vehicle and then treated with PE or blank control. The cells were observed using an optical microscope and stained or not stained with oil red. (C) The cholesterol level in cardiomyocytes was measured in response to PE. (D) The triglyceride level in cardiomyocytes was measured in response to PE. The data are presented as the means ± SEM of three independent experiments. *<i>P</i><0.05, **<i>P</i><0.01.</p>", "links"=>[], "tags"=>["nad", "acid oxidation", "wt", "attenuating lipid accumulation", "Sirt 3", "palmitate oxidation rates", "tac", "LCAD Cardiac hypertrophy", "lipid accumulation", "role", "hypertrophic hearts"], "article_id"=>1329089, "categories"=>["Biological Sciences"], "users"=>["Tongshuai Chen", "Junni Liu", "Na Li", "Shujian Wang", "Hui Liu", "Jingyuan Li", "Yun Zhang", "Peili Bu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118909.g006", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Overexpression_of_SIRT3_attenuated_lipid_accumulation_in_vitro_/1329089", "title"=>"Overexpression of SIRT3 attenuated lipid accumulation <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:34:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940553"], "description"=>"<p>(A) Expression level of SIRT3 in cardiomyocytes after infection with Ad.SIRT3 or vehicle at an MOI of 10 for 16 h. (B) Quantification of SIRT3 at different time points after infection with Ad.SIRT3. (C) Cardiomyocytes were infected with Ad.SIRT3 or vehicle and then treated with PE (20 μM) or blank control (saline). The cells were stained for α-SMA (red), and the nucleus positions were determined by DAPI staining (blue). (D) The incorporation of [<sup>3</sup>H]-leucine into total cellular proteins was determined and normalized to the DNA content of the cells. (E) mRNA levels of the indicated fetal genes (<i>anf</i> and <i>myh-7</i>) in cardiomyocytes infected with Ad.SIRT3 and then treated or not treated with PE. The data are presented as the means ± SEM of three independent experiments. *<i>P</i><0.05, **<i>P</i><0.01.</p>", "links"=>[], "tags"=>["nad", "acid oxidation", "wt", "attenuating lipid accumulation", "Sirt 3", "palmitate oxidation rates", "tac", "LCAD Cardiac hypertrophy", "lipid accumulation", "role", "hypertrophic hearts"], "article_id"=>1329087, "categories"=>["Biological Sciences"], "users"=>["Tongshuai Chen", "Junni Liu", "Na Li", "Shujian Wang", "Hui Liu", "Jingyuan Li", "Yun Zhang", "Peili Bu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0118909.g005", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Overexpression_of_SIRT3_blocked_the_cardiac_hypertrophic_response_in_vitro_/1329087", "title"=>"Overexpression of SIRT3 blocked the cardiac hypertrophic response <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:34:37"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"39", "full-text"=>"38", "pdf"=>"24", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"33", "full-text"=>"37", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"38", "full-text"=>"42", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"36", "full-text"=>"35", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"27", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"23", "full-text"=>"23", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"28", "full-text"=>"24", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"49", "full-text"=>"46", "pdf"=>"24", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"33", "full-text"=>"32", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"23", "full-text"=>"22", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"32", "full-text"=>"36", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"5", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"26", "full-text"=>"27", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"22", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"21", "full-text"=>"31", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"23", "full-text"=>"25", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"25", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts