Evidence of the Generation of Isosaccharinic Acids and Their Subsequent Degradation by Local Microbial Consortia within Hyper-Alkaline Contaminated Soils, with Relevance to Intermediate Level Radioactive Waste Disposal
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Evidence of the generation of isosaccharinic acids and their subsequent degradation by local microbial consortia within hyper-alkaline contaminated soils, with relevance to intermediate level radioactive waste disposal", "type"=>"journal", "authors"=>[{"first_name"=>"Simon P.", "last_name"=>"Rout", "scopus_author_id"=>"55303454600"}, {"first_name"=>"Christopher J.", "last_name"=>"Charles", "scopus_author_id"=>"7102123182"}, {"first_name"=>"Eva J.", "last_name"=>"Garratt", "scopus_author_id"=>"56840588800"}, {"first_name"=>"Andrew P.", "last_name"=>"Laws", "scopus_author_id"=>"7005825591"}, {"first_name"=>"John", "last_name"=>"Gunn", "scopus_author_id"=>"35620372100"}, {"first_name"=>"Paul N.", "last_name"=>"Humphreys", "scopus_author_id"=>"7005959780"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"602763525", "sgr"=>"84929094821", "issn"=>"19326203", "pmid"=>"25748643", "scopus"=>"2-s2.0-84929094821", "doi"=>"10.1371/journal.pone.0119164", "isbn"=>"1932-6203"}, "id"=>"0b0236e2-d244-3204-bfa0-d09c7357ae7c", "abstract"=>"The contamination of surface environments with hydroxide rich wastes leads to the formation of high pH (>11.0) soil profiles. One such site is a legacy lime works at Harpur Hill, Derbyshire where soil profile indicated in-situ pH values up to pH 12. Soil and porewater profiles around the site indicated clear evidence of the presence of the α and β stereoisomers of isosaccharinic acid (ISA) resulting from the anoxic, alkaline degradation of cellulosic material. ISAs are of particular interest with regards to the disposal of cellulosic materials contained within the intermediate level waste (ILW) inventory of the United Kingdom, where they may influence radionuclide mobility via complexation events occurring within a geological disposal facility (GDF) concept. The mixing of uncontaminated soils with the alkaline leachate of the site resulted in ISA generation, where the rate of generation in-situ is likely to be dependent upon the prevailing temperature of the soil. Microbial consortia present in the uncontaminated soil were capable of surviving conditions imposed by the alkaline leachate and demonstrated the ability to utilise ISAs as a carbon source. Leachate-contaminated soil was sub-cultured in a cellulose degradation product driven microcosm operating at pH 11, the consortia present were capable of the degradation of ISAs and the generation of methane from the resultant H2/CO2 produced from fermentation processes. Following microbial community analysis, fermentation processes appear to be predominated by Clostridia from the genus Alkaliphilus sp, with methanogenesis being attributed to Methanobacterium and Methanomassiliicoccus sp. The study is the first to identify the generation of ISA within an anthropogenic environment and advocates the notion that microbial activity within an ILW-GDF is likely to influence the impact of ISAs upon radionuclide migration.", "link"=>"http://www.mendeley.com/research/evidence-generation-isosaccharinic-acids-subsequent-degradation-local-microbial-consortia-within-hyp", "reader_count"=>18, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>9, "Student > Ph. D. Student"=>3, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>9, "Student > Ph. D. Student"=>3, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Environmental Science"=>6, "Nursing and Health Professions"=>1, "Materials Science"=>2, "Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>3, "Chemistry"=>1, "Earth and Planetary Sciences"=>2}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Unspecified"=>{"Unspecified"=>2}, "Environmental Science"=>{"Environmental Science"=>6}}, "reader_count_by_country"=>{"Germany"=>1}, "group_count"=>3}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84929094821"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84929094821?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84929094821&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84929094821&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84929094821", "dc:identifier"=>"SCOPUS_ID:84929094821", "eid"=>"2-s2.0-84929094821", "dc:title"=>"Evidence of the generation of isosaccharinic acids and their subsequent degradation by local microbial consortia within hyper-alkaline contaminated soils, with relevance to intermediate level radioactive waste disposal", "dc:creator"=>"Rout S.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-03-06", "prism:coverDisplayDate"=>"6 March 2015", "prism:doi"=>"10.1371/journal.pone.0119164", "citedby-count"=>"15", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Huddersfield", "affiliation-city"=>"Huddersfield", "affiliation-country"=>"United Kingdom"}], "pubmed-id"=>"25748643", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0119164", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0119164", "share_count"=>1, "like_count"=>3, "comment_count"=>0, "click_count"=>0, "total_count"=>4}

Counter

  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"30", "xml_views"=>"6", "html_views"=>"308"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"67"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"21"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"5", "html_views"=>"10"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"3", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"20", "xml_views"=>"2", "html_views"=>"4"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"13"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1940476"], "description"=>"<p>Arrhenius plot of aqueous ISA generation from uncontaminated soils in alkaline leachate.</p>", "links"=>[], "tags"=>["uncontaminated soils", "ISA generation", "legacy lime works", "β stereoisomers", "radionuclide mobility", "Local Microbial Consortia", "level waste", "uncontaminated soil", "genus Alkaliphilus sp", "Intermediate Level Radioactive Waste Disposal", "Microbial consortia", "Methanomassiliicoccus sp", "disposal facility", "carbon source", "porewater profiles", "utilise ISAs", "Subsequent Degradation", "ILW", "Harpur Hill", "isosaccharinic acid", "anthropogenic environment", "complexation events", "gdf", "Isosaccharinic Acids", "soil profile", "site", "pH 12. Soil", "cellulosic material", "surface environments", "cellulose degradation product", "radionuclide migration", "community analysis", "cellulosic materials"], "article_id"=>1329024, "categories"=>["Biological Sciences", "Science Policy"], "users"=>["Simon P. Rout", "Christopher J. Charles", "Eva J. Garratt", "Andrew P. Laws", "John Gunn", "Paul N. Humphreys"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119164.g004", "stats"=>{"downloads"=>2, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Arrhenius_plot_of_aqueous_ISA_generation_from_uncontaminated_soils_in_alkaline_leachate_/1329024", "title"=>"Arrhenius plot of aqueous ISA generation from uncontaminated soils in alkaline leachate.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:27:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940474"], "description"=>"<p>Although the presence of ISA is not observed in the natural environment, previous research has shown that micro-organisms from neutral sediments are capable of the utilisation of both α and β ISA within microcosm experiments. No significant difference in ISA degradation rates were observed across microcosms operating under iron reducing, sulphate reducing and methanogenic conditions at pH 7.5 [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119164#pone.0119164.ref010\" target=\"_blank\">10</a>]. When methanogenic sediments were subjected to further increases in pH (up to pH 10), the overall ISA degradation rate was slower. In addition, a difference was seen in the degradation rates of α and β ISA suggesting the potential for the persistence of the β stereoisomer within and surrounding the chemically disturbed zone of an ILW-GDF [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119164#pone.0119164.ref011\" target=\"_blank\">11</a>]. These communities were incapable of surviving at a pH of 11.0, suggesting that longer timescales may be required for micro-organisms to adapt to environmental pH values above pH 10.0, rather than adapting an ability to degrade ISA. In view of this, the hyper alkaline environment present at Harpur Hill provides an alkaliphilic soil dwelling microbial consortia which may be more indicative of the consortia that may develop with the near-field of an ILW-GDF.</p>", "links"=>[], "tags"=>["uncontaminated soils", "ISA generation", "legacy lime works", "β stereoisomers", "radionuclide mobility", "Local Microbial Consortia", "level waste", "uncontaminated soil", "genus Alkaliphilus sp", "Intermediate Level Radioactive Waste Disposal", "Microbial consortia", "Methanomassiliicoccus sp", "disposal facility", "carbon source", "porewater profiles", "utilise ISAs", "Subsequent Degradation", "ILW", "Harpur Hill", "isosaccharinic acid", "anthropogenic environment", "complexation events", "gdf", "Isosaccharinic Acids", "soil profile", "site", "pH 12. Soil", "cellulosic material", "surface environments", "cellulose degradation product", "radionuclide migration", "community analysis", "cellulosic materials"], "article_id"=>1329022, "categories"=>["Biological Sciences", "Science Policy"], "users"=>["Simon P. Rout", "Christopher J. Charles", "Eva J. Garratt", "Andrew P. Laws", "John Gunn", "Paul N. Humphreys"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119164.g002", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_945_L_and_946_R_conformations_of_isosaccharinic_acid_ISA_/1329022", "title"=>"The α- (L) and β- (R) conformations of isosaccharinic acid (ISA).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:27:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940479"], "description"=>"<div><p>The contamination of surface environments with hydroxide rich wastes leads to the formation of high pH (>11.0) soil profiles. One such site is a legacy lime works at Harpur Hill, Derbyshire where soil profile indicated <i>in-situ</i> pH values up to pH 12. Soil and porewater profiles around the site indicated clear evidence of the presence of the α and β stereoisomers of isosaccharinic acid (ISA) resulting from the anoxic, alkaline degradation of cellulosic material. ISAs are of particular interest with regards to the disposal of cellulosic materials contained within the intermediate level waste (ILW) inventory of the United Kingdom, where they may influence radionuclide mobility via complexation events occurring within a geological disposal facility (GDF) concept. The mixing of uncontaminated soils with the alkaline leachate of the site resulted in ISA generation, where the rate of generation <i>in-situ</i> is likely to be dependent upon the prevailing temperature of the soil. Microbial consortia present in the uncontaminated soil were capable of surviving conditions imposed by the alkaline leachate and demonstrated the ability to utilise ISAs as a carbon source. Leachate-contaminated soil was sub-cultured in a cellulose degradation product driven microcosm operating at pH 11, the consortia present were capable of the degradation of ISAs and the generation of methane from the resultant H<sub>2</sub>/CO<sub>2</sub> produced from fermentation processes. Following microbial community analysis, fermentation processes appear to be predominated by Clostridia from the genus <i>Alkaliphilus</i> sp, with methanogenesis being attributed to <i>Methanobacterium</i> and <i>Methanomassiliicoccus</i> sp. The study is the first to identify the generation of ISA within an anthropogenic environment and advocates the notion that microbial activity within an ILW-GDF is likely to influence the impact of ISAs upon radionuclide migration.</p></div>", "links"=>[], "tags"=>["uncontaminated soils", "ISA generation", "legacy lime works", "β stereoisomers", "radionuclide mobility", "Local Microbial Consortia", "level waste", "uncontaminated soil", "genus Alkaliphilus sp", "Intermediate Level Radioactive Waste Disposal", "Microbial consortia", "Methanomassiliicoccus sp", "disposal facility", "carbon source", "porewater profiles", "utilise ISAs", "Subsequent Degradation", "ILW", "Harpur Hill", "isosaccharinic acid", "anthropogenic environment", "complexation events", "gdf", "Isosaccharinic Acids", "soil profile", "site", "pH 12. Soil", "cellulosic material", "surface environments", "cellulose degradation product", "radionuclide migration", "community analysis", "cellulosic materials"], "article_id"=>1329027, "categories"=>["Biological Sciences", "Science Policy"], "users"=>["Simon P. Rout", "Christopher J. Charles", "Eva J. Garratt", "Andrew P. Laws", "John Gunn", "Paul N. Humphreys"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119164", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Evidence_of_the_Generation_of_Isosaccharinic_Acids_and_Their_Subsequent_Degradation_by_Local_Microbial_Consortia_within_Hyper_Alkaline_Contaminated_Soils_with_Relevance_to_Intermediate_Level_Radioactive_Waste_Disposal_/1329027", "title"=>"Evidence of the Generation of Isosaccharinic Acids and Their Subsequent Degradation by Local Microbial Consortia within Hyper-Alkaline Contaminated Soils, with Relevance to Intermediate Level Radioactive Waste Disposal", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-03-06 09:27:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940478"], "description"=>"<p>Eubacterial (A, n = 32) and Archaeal (B, n = 31) 16S rRNA gene clone libraries of pH 11 CDP driven microcosm where clones were assigned to a family based on the closest sequence match obtained through MegaBLAST database search.</p>", "links"=>[], "tags"=>["uncontaminated soils", "ISA generation", "legacy lime works", "β stereoisomers", "radionuclide mobility", "Local Microbial Consortia", "level waste", "uncontaminated soil", "genus Alkaliphilus sp", "Intermediate Level Radioactive Waste Disposal", "Microbial consortia", "Methanomassiliicoccus sp", "disposal facility", "carbon source", "porewater profiles", "utilise ISAs", "Subsequent Degradation", "ILW", "Harpur Hill", "isosaccharinic acid", "anthropogenic environment", "complexation events", "gdf", "Isosaccharinic Acids", "soil profile", "site", "pH 12. Soil", "cellulosic material", "surface environments", "cellulose degradation product", "radionuclide migration", "community analysis", "cellulosic materials"], "article_id"=>1329026, "categories"=>["Biological Sciences", "Science Policy"], "users"=>["Simon P. Rout", "Christopher J. Charles", "Eva J. Garratt", "Andrew P. Laws", "John Gunn", "Paul N. Humphreys"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119164.g006", "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Eubacterial_A_n_32_and_Archaeal_B_n_31_16S_rRNA_gene_clone_libraries_of_pH_11_CDP_driven_microcosm_where_clones_were_assigned_to_a_family_based_on_the_closest_sequence_match_obtained_through_MegaBLAST_database_search_/1329026", "title"=>"Eubacterial (A, n = 32) and Archaeal (B, n = 31) 16S rRNA gene clone libraries of pH 11 CDP driven microcosm where clones were assigned to a family based on the closest sequence match obtained through MegaBLAST database search.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:27:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940473"], "description"=>"<p>One design concept for the long term storage of the United Kingdom’s intermediate level radioactive waste (ILW) inventory is a geological disposal facility (GDF) with a cementitious backfill [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119164#pone.0119164.ref004\" target=\"_blank\">4</a>]. Under the alkaline (10 < pH > 13), anoxic and saturated conditions predicted to develop following the closure of such a facility the cellulosic materials present in the waste [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119164#pone.0119164.ref005\" target=\"_blank\">5</a>] will undergo alkaline hydrolysis [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119164#pone.0119164.ref006\" target=\"_blank\">6</a>]. This process generates a range of organic compounds dominated by the α and β stereoisomers of isosaccharinic acid (ISA) (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119164#pone.0119164.g002\" target=\"_blank\">Fig. 2</a>) [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119164#pone.0119164.ref006\" target=\"_blank\">6</a>,<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119164#pone.0119164.ref007\" target=\"_blank\">7</a>]. ISA is of relevance to radioactive waste disposal since it is able to form complexes with certain radionuclides, enhancing their migration and consequently ISA may influence the performance of an ILW GDF [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119164#pone.0119164.ref008\" target=\"_blank\">8</a>,<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119164#pone.0119164.ref009\" target=\"_blank\">9</a>].</p>", "links"=>[], "tags"=>["uncontaminated soils", "ISA generation", "legacy lime works", "β stereoisomers", "radionuclide mobility", "Local Microbial Consortia", "level waste", "uncontaminated soil", "genus Alkaliphilus sp", "Intermediate Level Radioactive Waste Disposal", "Microbial consortia", "Methanomassiliicoccus sp", "disposal facility", "carbon source", "porewater profiles", "utilise ISAs", "Subsequent Degradation", "ILW", "Harpur Hill", "isosaccharinic acid", "anthropogenic environment", "complexation events", "gdf", "Isosaccharinic Acids", "soil profile", "site", "pH 12. Soil", "cellulosic material", "surface environments", "cellulose degradation product", "radionuclide migration", "community analysis", "cellulosic materials"], "article_id"=>1329021, "categories"=>["Biological Sciences", "Science Policy"], "users"=>["Simon P. Rout", "Christopher J. Charles", "Eva J. Garratt", "Andrew P. Laws", "John Gunn", "Paul N. Humphreys"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119164.g001", "stats"=>{"downloads"=>4, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Site_overview_/1329021", "title"=>"Site overview.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:27:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940477"], "description"=>"<p>Chemistry of CDP driven microcosms operating at pH 11.0 using site contaminated soil as a starting inoculum.</p>", "links"=>[], "tags"=>["uncontaminated soils", "ISA generation", "legacy lime works", "β stereoisomers", "radionuclide mobility", "Local Microbial Consortia", "level waste", "uncontaminated soil", "genus Alkaliphilus sp", "Intermediate Level Radioactive Waste Disposal", "Microbial consortia", "Methanomassiliicoccus sp", "disposal facility", "carbon source", "porewater profiles", "utilise ISAs", "Subsequent Degradation", "ILW", "Harpur Hill", "isosaccharinic acid", "anthropogenic environment", "complexation events", "gdf", "Isosaccharinic Acids", "soil profile", "site", "pH 12. Soil", "cellulosic material", "surface environments", "cellulose degradation product", "radionuclide migration", "community analysis", "cellulosic materials"], "article_id"=>1329025, "categories"=>["Biological Sciences", "Science Policy"], "users"=>["Simon P. Rout", "Christopher J. Charles", "Eva J. Garratt", "Andrew P. Laws", "John Gunn", "Paul N. Humphreys"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119164.g005", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Chemistry_of_CDP_driven_microcosms_operating_at_pH_11_0_using_site_contaminated_soil_as_a_starting_inoculum_/1329025", "title"=>"Chemistry of CDP driven microcosms operating at pH 11.0 using site contaminated soil as a starting inoculum.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:27:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1940475"], "description"=>"<p>Presence of ISA’s abiotic reactions (closed diamond) and biotic reactions (open squares) involving uncontaminated soil and alkaline leachate from the site at 4°C (A), 10°C (B) and 20°C (C, n = 3).</p>", "links"=>[], "tags"=>["uncontaminated soils", "ISA generation", "legacy lime works", "β stereoisomers", "radionuclide mobility", "Local Microbial Consortia", "level waste", "uncontaminated soil", "genus Alkaliphilus sp", "Intermediate Level Radioactive Waste Disposal", "Microbial consortia", "Methanomassiliicoccus sp", "disposal facility", "carbon source", "porewater profiles", "utilise ISAs", "Subsequent Degradation", "ILW", "Harpur Hill", "isosaccharinic acid", "anthropogenic environment", "complexation events", "gdf", "Isosaccharinic Acids", "soil profile", "site", "pH 12. Soil", "cellulosic material", "surface environments", "cellulose degradation product", "radionuclide migration", "community analysis", "cellulosic materials"], "article_id"=>1329023, "categories"=>["Biological Sciences", "Science Policy"], "users"=>["Simon P. Rout", "Christopher J. Charles", "Eva J. Garratt", "Andrew P. Laws", "John Gunn", "Paul N. Humphreys"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119164.g003", "stats"=>{"downloads"=>1, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Presence_of_ISA_8217_s_abiotic_reactions_closed_diamond_and_biotic_reactions_open_squares_involving_uncontaminated_soil_and_alkaline_leachate_from_the_site_at_4_176_C_A_10_176_C_B_and_20_176_C_C_n_3_/1329023", "title"=>"Presence of ISA’s abiotic reactions (closed diamond) and biotic reactions (open squares) involving uncontaminated soil and alkaline leachate from the site at 4°C (A), 10°C (B) and 20°C (C, n = 3).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-06 09:27:57"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"24", "full-text"=>"19", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"20", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"5", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"18", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"20", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts