Comparative Distribution and In Vitro Activities of the Urotensin II-Related Peptides URP1 and URP2 in Zebrafish: Evidence for Their Colocalization in Spinal Cerebrospinal Fluid-Contacting Neurons
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Comparative distribution and in vitro activities of the urotensin II-related peptides URP1 and URP2 in zebrafish: Evidence for their colocalization in spinal cerebrospinal fluid-contacting neurons", "type"=>"journal", "authors"=>[{"first_name"=>"Feng B.", "last_name"=>"Quan", "scopus_author_id"=>"39762588000"}, {"first_name"=>"Christophe", "last_name"=>"Dubessy", "scopus_author_id"=>"6507064990"}, {"first_name"=>"Sonya", "last_name"=>"Galant", "scopus_author_id"=>"55808449100"}, {"first_name"=>"Natalia B.", "last_name"=>"Kenigfest", "scopus_author_id"=>"6701338403"}, {"first_name"=>"Lydia", "last_name"=>"Djenoune", "scopus_author_id"=>"56149489600"}, {"first_name"=>"Jérôme", "last_name"=>"Leprince", "scopus_author_id"=>"6603815221"}, {"first_name"=>"Claire", "last_name"=>"Wyart", "scopus_author_id"=>"6507983723"}, {"first_name"=>"Isabelle", "last_name"=>"Lihrmann", "scopus_author_id"=>"7003495688"}, {"first_name"=>"Hervé", "last_name"=>"Tostivint", "scopus_author_id"=>"35447989400"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84924940474", "sgr"=>"84924940474", "pui"=>"603051554", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"25781313", "doi"=>"10.1371/journal.pone.0119290"}, "id"=>"8de8408d-e3d8-3d3d-9ab0-7cfb7fc58100", "abstract"=>"Urotensin II (UII) is an evolutionarily conserved neuropeptide initially isolated from teleost fish on the basis of its smooth muscle-contracting activity. Subsequent studies have demonstrated the occurrence of several UII-related peptides (URPs), such that the UII family is now known to include four paralogue genes called UII, URP, URP1 and URP2. These genes probably arose through the two rounds of whole genome duplication that occurred during early vertebrate evolution. URP has been identified both in tetrapods and teleosts. In contrast, URP1 and URP2 have only been observed in ray-finned and cartilaginous fishes, suggesting that both genes were lost in the tetrapod lineage. In the present study, the distribution of urp1 mRNA compared to urp2 mRNA is reported in the central nervous system of zebrafish. In the spinal cord, urp1 and urp2 mRNAs were mainly colocalized in the same cells. These cells were also shown to be GABAergic and express the gene encoding the polycystic kidney disease 2-like 1 (pkd2l1) channel, indicating that they likely correspond to cerebrospinal fluid-contacting neurons. In the hindbrain, urp1-expressing cells were found in the intermediate reticular formation and the glossopharyngeal-vagal motor nerve nuclei. We also showed that synthetic URP1 and URP2 were able to induce intracellular calcium mobilization in human UII receptor (hUT)-transfected CHO cells with similar potencies (pEC50=7.99 and 7.52, respectively) albeit at slightly lower potencies than human UII and mammalian URP (pEC50=9.44 and 8.61, respectively). The functional redundancy of URP1 and URP2 as well as the colocalization of their mRNAs in the spinal cord suggest the robustness of this peptidic system and its physiological importance in zebrafish.", "link"=>"http://www.mendeley.com/research/comparative-distribution-vitro-activities-urotensin-iirelated-peptides-urp1-urp2-zebrafish-evidence", "reader_count"=>16, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>4, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Student > Bachelor"=>1, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>4}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>4, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Student > Bachelor"=>1, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>4}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>4, "Nursing and Health Professions"=>1, "Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>4, "Neuroscience"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>3}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84924940474"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84924940474?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84924940474&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84924940474&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84924940474", "dc:identifier"=>"SCOPUS_ID:84924940474", "eid"=>"2-s2.0-84924940474", "dc:title"=>"Comparative distribution and in vitro activities of the urotensin II-related peptides URP1 and URP2 in zebrafish: Evidence for their colocalization in spinal cerebrospinal fluid-contacting neurons", "dc:creator"=>"Quan F.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-03-17", "prism:coverDisplayDate"=>"17 March 2015", "prism:doi"=>"10.1371/journal.pone.0119290", "citedby-count"=>"10", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Museum National d'Histoire Naturelle", "affiliation-city"=>"Paris", "affiliation-country"=>"France"}], "pubmed-id"=>"25781313", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0119290", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0119290", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"158"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"3", "html_views"=>"56"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"42"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"77"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"95"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1952930"], "description"=>"<p>Representative dose-response curves of <i>h</i>UII (●), <i>m</i>URP (■), URP1 (▲) and URP2 (▼) on the intracellular calcium mobilization <b>(A)</b>. The values are expressed as percentages of the baseline and each point is the mean (± S.E.M.) of 3 replicates. Experimental data were fitted using a four-parameter logistic equation. The potencies of 7–13 independent experiments for each peptides were plotted as—Log(EC<sub>50</sub>) with box and whiskers <b>(B)</b>. Values were considered as statistically different as assessed by analysis of variance followed by Tukey’s post-test, n.s., not significant, *<i>p</i> < 0.05, **<i>p</i> < 0.01, ***<i>p</i> < 0.001, ****<i>p</i> < 0.0001.</p>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338912, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119290.g011", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_URP1_and_URP2_are_equipotent_to_induce_intracellular_calcium_mobilization_in_a_h_UT_transfected_CHO_cell_line_/1338912", "title"=>"URP1 and URP2 are equipotent to induce intracellular calcium mobilization in a <i>h</i>UT-transfected CHO cell line.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952903"], "description"=>"<p>Expression of <i>urp1</i> revealed by ISH (BM purple, violet) on nacre embryos at 22 <b>(A)</b>, 28 <b>(B)</b> and 48 hpf <b>(C)</b>. At 22 hpf and 28 hpf, <i>urp1</i><sup>+</sup> cells occur only in the spinal cord at the level of the lateral floor plate <b>(A, B)</b>, while from 48 hpf, they are mainly visible in the hindbrain <b>(C)</b>. <b>A1</b>, <b>B1</b>, <b>B5</b> and <b>C1</b>, dorsal views; <b>A2</b>, <b>B2, B3</b> and <b>C2</b>, lateral views with dorsal up; <b>B4</b>, coronal section with dorsal up; all embryos oriented anterior left; <b>B3</b> and <b>B5</b> are details at higher magnifications of B2 and B1, respectively. Ch, chord; Cc, central canal; Nt, neural tube; Scale bar: 100 μm.</p>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338888, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119290.g001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_urp1_mRNA_is_restricted_to_the_ventral_spinal_cord_and_hindbrain_at_early_stages_of_development_in_zebrafish_/1338888", "title"=>"<i>urp1</i> mRNA is restricted to the ventral spinal cord and hindbrain at early stages of development in zebrafish.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952904"], "description"=>"<p>Tissue distribution of <i>urp1</i> and <i>urp2</i> mRNAs assessed by RT-PCR. Parallel amplification of zebrafish β-actin mRNA served as internal control. NTC, non-template control.</p>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338889, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119290.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_urp1_and_urp2_mRNAs_are_exclusively_detected_in_the_brain_and_spinal_cord_in_adult_zebrafish_/1338889", "title"=>"<i>urp1</i> and <i>urp2</i> mRNAs are exclusively detected in the brain and spinal cord in adult zebrafish.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952906"], "description"=>"<p>Expression of <i>urp1</i> revealed by fluorescent ISH (FITC, green) on coronal sections of adult brain <b>(A)</b>. <i>urp1</i> mRNA is visible in neurons located in the intermediate reticular formation <b>(A1</b>) and the region of the glossopharyngeal-vagal motor nerve nuclei (<b>A2–A3</b>). More caudally, at the level of the junction between hindbrain and spinal cord, <i>urp1</i> mRNA occurs at the ventral edge of the central canal <b>(A4)</b>. Schematic sagittal view of an adult zebrafish brain depicting the distribution of <i>urp1</i> mRNA (red dots). Levels of sections shown in A are indicated. The anatomical structures are designated according to [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119290#pone.0119290.ref038\" target=\"_blank\">38</a>] <b>(B)</b>. CC, cerebellar crest; C, central canal; CCe, corpus cerebelli; DON, dorsal octavolateralis nucleus; EW, Edinger-Westphal nucleus; FLo, facial lobe; Ha, habenula; H, hypothalamus; IMRF, intermediate reticular formation; MO, medulla oblongata; NC, commissural nucleus of Cajal; nIX-X, glossopharyngeal-vagal motor nerve nuclei; OB, olfactive bulbs; OC, optic chiasma; P, pallium; PN, preopic nucleus; RV, rhombencephalic ventricle; SCsm, spinal cord somatomotor neurons; SP, subpallium; T, thalamus; TO, tectum opticum; TL, torus longitudinalis; TP, posterior tuberculum; TS, torus semicircularis; VLo, vagal lobe. Scale bars: 100 μm.</p>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338891, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119290.g003", "stats"=>{"downloads"=>4, "page_views"=>119, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_urp1_mRNA_is_found_in_the_caudal_part_of_the_hindbrain_in_adult_zebrafish_/1338891", "title"=>"<i>urp1</i> mRNA is found in the caudal part of the hindbrain in adult zebrafish.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952912"], "description"=>"<p>Expression of <i>urp1</i> revealed by ISH (BM purple, violet) on free-floating sections of adult spinal cord. <i>urp1</i><sup>+</sup> cells form a quasi-continuous line at the ventral edge of the central canal <b>(A)</b>. <i>urp1</i><sup>+</sup> cells are in close contact to the lumen of the central canal (arrowhead) <b>(B)</b>. <b>A1</b> and <b>A2</b>, lateral sections with dorsal up; <b>B</b>, coronal section with dorsal up. <i>urp1</i><sup>+</sup> cells boxed in <b>A1</b> are shown in <b>A2</b> at higher magnification. M, melanocytes. Scale bars: 50 μm.</p>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338897, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119290.g004", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_urp1_mRNA_occurs_in_cells_located_along_the_ventral_edge_of_the_central_canal_of_spinal_cord_in_adult_zebrafish_/1338897", "title"=>"<i>urp1</i> mRNA occurs in cells located along the ventral edge of the central canal of spinal cord in adult zebrafish.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952913"], "description"=>"<p>Simultaneous expression of <i>urp1</i> and <i>urp2</i> revealed by double fluorescent ISH (TAMRA, red for <i>urp1</i>, FITC, green for <i>urp2</i> and DAPI in blue) on 24 hpf-embryo <b>(A)</b> and adult spinal cord sections <b>(B, C)</b>. In embryo, all the stained cells contain both <i>urp1</i> and <i>urp2</i> mRNAs <b>(A)</b>. In adult, although most of the stained cells are doubly-positive for <i>urp1</i> and <i>urp2</i> (arrow), some of the <i>urp2</i><sup>+</sup> cells are devoid of any <i>urp1</i> mRNA (arrowhead). The white dash line indicates the central canal. <b>A</b>, dorsal view; <b>B</b>, sagittal section with dorsal up; <b>C</b>, coronal section with dorsal up. Scale bars: 15 μm.</p>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338898, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119290.g005", "stats"=>{"downloads"=>4, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_urp1_and_urp2_mRNAs_are_mainly_coexpressed_in_the_same_spinal_cord_cells_in_zebrafish_/1338898", "title"=>"<i>urp1</i> and <i>urp2</i> mRNAs are mainly coexpressed in the same spinal cord cells in zebrafish.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952914"], "description"=>"<p><i>urp1</i><b>(A)</b> and <i>urp2</i><b>(B)</b> expression revealed by fluorescent ISH (FITC, green) on 24 hpf-embryo, together with a fluorescent immunostaining for GAD<sub>65/67</sub> (Alexa Fluor 546, red). Both <i>urp1</i><sup>+</sup> and <i>urp2</i><sup>+</sup> cells are GAD<sup>+</sup> (arrows). Note that only ventral KA (KA”) cells are doubly stained. In contrast, dorsal KA (KA’) cells are GAD<sup>+</sup> but do not express <i>urp1</i> (arrowhead). The white dash line indicates the central canal. <b>A</b> and <b>B</b>, coronal sections with dorsal up. Scale bars: 15 μm.</p>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338899, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119290.g006", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Both_urp1_and_urp2_cells_are_GABAergic_neurons_in_the_zebrafish_embryo_/1338899", "title"=>"Both <i>urp1</i><sup>+</sup> and <i>urp2</i><sup>+</sup> cells are GABAergic neurons in the zebrafish embryo.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952915"], "description"=>"<p>Simultaneous expression of <i>urp1</i> and <i>urp2</i> revealed by double fluorescent ISH (TAMRA, red for <i>urp1</i> and Cy5, white for <i>urp2</i>) in adult spinal cord, coupled to a fluorescent immunostaining for GAD<sub>65/67</sub> (Alexa Fluor 488, green) <b>(A</b>, <b>B)</b>. Both <i>urp1</i><sup>+</sup> and <i>urp2</i><sup>+</sup> cells are GAD<sup>+</sup><b>(A, B)</b>. Arrows designate triple-stained cells <b>(A, B)</b>. Note the occurrence of some doubly-positive cells (<i>urp2</i>/GAD) that do not contain any <i>urp1</i> (arrowhead) <b>(A)</b>. Asterisks designate GABAergic interneurons located at the dorsal part of the spinal cord. The white dash line indicates the central canal. <b>A</b>, sagittal section with dorsal up; <b>B</b>, coronal section with dorsal up. Scale bars: 15 μm.</p>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338900, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119290.g007", "stats"=>{"downloads"=>4, "page_views"=>80, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Both_urp1_and_urp2_cells_in_the_spinal_cord_are_GABAergic_neurons_in_adult_zebrafish_/1338900", "title"=>"Both <i>urp1</i><sup>+</sup> and <i>urp2</i><sup>+</sup> cells in the spinal cord are GABAergic neurons in adult zebrafish.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952919"], "description"=>"<p>Simultaneous expression of <i>urp1</i> and <i>pkd2l1</i> revealed by double fluorescent ISH (TAMRA, red for <i>urp1</i> and FITC, green for <i>pkd2l1</i>) on 24 hpf-embryo <b>(A)</b> and adult spinal cord sections <b>(B)</b>. <i>pkd2l1</i> mRNA is distributed in two rows of cells along the rostro-caudal axis of the spinal cord both in embryo and adult <b>(A2, B2)</b>. All the <i>urp1</i><sup>+</sup> cells are <i>pkd2l1</i><sup>+</sup><b>(A1,3</b>, <b>B1,3)</b> but only the ventral <i>pkd2l1</i><sup>+</sup> cells are <i>urp1</i><sup>+</sup>. The white dash line indicates the central canal. <b>A</b>, lateral views; <b>B</b>, sagittal sections with dorsal up. Scale bars: 20μm.</p>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338902, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119290.g008", "stats"=>{"downloads"=>4, "page_views"=>35, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_urp1_cells_express_pkd2l1_a_specific_marker_of_spinal_cerebrospinal_fluid_contacting_neurons_in_zebrafish_/1338902", "title"=>"<i>urp1</i><sup>+</sup> cells express <i>pkd2l1</i>, a specific marker of spinal cerebrospinal fluid- contacting neurons in zebrafish.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952923"], "description"=>"<p>Simultaneous expression of <i>urp1</i> and <i>isl1</i><b>(A)</b> or <i>ss1</i><b>(B)</b> revealed by double fluorescent ISH (TAMRA, red for <i>urp1</i> and FITC, green for <i>isl1</i> or <i>ss1</i>) on 48 hpf-embryo. <i>urp1</i><sup>+</sup> cells are located at the level of the medial motor nucleus of the vagus. Most of them appear to be both <i>isl1</i><sup>+</sup> and <i>ss1</i><sup>+</sup>. All pictures are dorsal views with anterior left. The boxed region in <b>A1</b> is shown at higher magnification in <b>A2–A4</b> and the same region is shown in <b>B</b>. nV, trigeminal nerve motor nuclei; nVII, facial nerve motor nuclei; nX, vagal nerve motor nuclei. Scale bars: 20 μm.</p>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338905, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119290.g009", "stats"=>{"downloads"=>1, "page_views"=>80, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_urp1_cells_express_isl1_and_ss1_two_markers_of_the_vagus_motor_nucleus_in_zebrafish_embryo_/1338905", "title"=>"<i>urp1</i><sup>+</sup> cells express <i>isl1</i> and <i>ss1</i>, two markers of the vagus motor nucleus in zebrafish embryo.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952966", "https://ndownloader.figshare.com/files/1952967", "https://ndownloader.figshare.com/files/1952968"], "description"=>"<div><p>Urotensin II (UII) is an evolutionarily conserved neuropeptide initially isolated from teleost fish on the basis of its smooth muscle-contracting activity. Subsequent studies have demonstrated the occurrence of several UII-related peptides (URPs), such that the UII family is now known to include four paralogue genes called <i>UII</i>, <i>URP</i>, <i>URP1</i> and <i>URP2</i>. These genes probably arose through the two rounds of whole genome duplication that occurred during early vertebrate evolution. <i>URP</i> has been identified both in tetrapods and teleosts. In contrast, <i>URP1</i> and <i>URP2</i> have only been observed in ray-finned and cartilaginous fishes, suggesting that both genes were lost in the tetrapod lineage. In the present study, the distribution of <i>urp1</i> mRNA compared to <i>urp2</i> mRNA is reported in the central nervous system of zebrafish. In the spinal cord, <i>urp1</i> and <i>urp2</i> mRNAs were mainly colocalized in the same cells. These cells were also shown to be GABAergic and express the gene encoding the polycystic kidney disease 2-like 1 (pkd2l1) channel, indicating that they likely correspond to cerebrospinal fluid-contacting neurons. In the hindbrain, <i>urp1</i>-expressing cells were found in the intermediate reticular formation and the glossopharyngeal-vagal motor nerve nuclei. We also showed that synthetic URP1 and URP2 were able to induce intracellular calcium mobilization in human UII receptor (<i>h</i>UT)-transfected CHO cells with similar potencies (pEC50=7.99 and 7.52, respectively) albeit at slightly lower potencies than human UII and mammalian URP (pEC50=9.44 and 8.61, respectively). The functional redundancy of URP1 and URP2 as well as the colocalization of their mRNAs in the spinal cord suggest the robustness of this peptidic system and its physiological importance in zebrafish.</p></div>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338940, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0119290.s001", "https://dx.doi.org/10.1371/journal.pone.0119290.s002", "https://dx.doi.org/10.1371/journal.pone.0119290.s003"], "stats"=>{"downloads"=>8, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Comparative_Distribution_and_In_Vitro_Activities_of_the_Urotensin_II_Related_Peptides_URP1_and_URP2_in_Zebrafish_Evidence_for_Their_Colocalization_in_Spinal_Cerebrospinal_Fluid_Contacting_Neurons/1338940", "title"=>"Comparative Distribution and <i>In Vitro</i> Activities of the Urotensin II-Related Peptides URP1 and URP2 in Zebrafish: Evidence for Their Colocalization in Spinal Cerebrospinal Fluid-Contacting Neurons", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-03-17 04:19:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1952927"], "description"=>"<p><i>urp1</i> expression revealed by fluorescent ISH (TAMRA, red) on coronal sections of adult brain, together with a fluorescent immunostaining for ChAT (Alexa 488, green). <i>urp1</i><sup>+</sup> cells express ChAT. Scale bars: 100 μm.</p>", "links"=>[], "tags"=>["uii", "urp 1 mRNA", "cho", "urp 2 mRNAs", "pkd 2l channel", "urp 2 mRNA", "intracellular calcium mobilization", "ii", "URP 2", "URP 1"], "article_id"=>1338909, "categories"=>["Biological Sciences"], "users"=>["Feng B. Quan", "Christophe Dubessy", "Sonya Galant", "Natalia B. Kenigfest", "Lydia Djenoune", "Jérôme Leprince", "Claire Wyart", "Isabelle Lihrmann", "Hervé Tostivint"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0119290.g010", "stats"=>{"downloads"=>5, "page_views"=>277, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_urp1_cells_in_the_hindbrain_are_cholinergic_neurons_expressing_ChAT_in_adult_zebrafish_/1338909", "title"=>"<i>urp1</i><sup>+</sup> cells in the hindbrain are cholinergic neurons expressing ChAT in adult zebrafish.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-17 04:19:38"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"30", "full-text"=>"34", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"21", "full-text"=>"24", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"22", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"24", "full-text"=>"24", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"28", "full-text"=>"24", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"30", "full-text"=>"23", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"20", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts