A Novel Host-Proteome Signature for Distinguishing between Acute Bacterial and Viral Infections
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"A novel host-proteome signature for distinguishing between acute bacterial and viral infections", "type"=>"journal", "authors"=>[{"first_name"=>"Kfir", "last_name"=>"Oved", "scopus_author_id"=>"8613197800"}, {"first_name"=>"Asi", "last_name"=>"Cohen", "scopus_author_id"=>"56567131200"}, {"first_name"=>"Olga", "last_name"=>"Boico", "scopus_author_id"=>"57191409606"}, {"first_name"=>"Roy", "last_name"=>"Navon", "scopus_author_id"=>"24473222000"}, {"first_name"=>"Tom", "last_name"=>"Friedman", "scopus_author_id"=>"56567618000"}, {"first_name"=>"Liat", "last_name"=>"Etshtein", "scopus_author_id"=>"56568230400"}, {"first_name"=>"Or", "last_name"=>"Kriger", "scopus_author_id"=>"57188715287"}, {"first_name"=>"Ellen", "last_name"=>"Bamberger", "scopus_author_id"=>"8503048400"}, {"first_name"=>"Yura", "last_name"=>"Fonar", "scopus_author_id"=>"56568206500"}, {"first_name"=>"Renata", "last_name"=>"Yacobov", "scopus_author_id"=>"16178565100"}, {"first_name"=>"Ron", "last_name"=>"Wolchinsky", "scopus_author_id"=>"26639864000"}, {"first_name"=>"Galit", "last_name"=>"Denkberg", "scopus_author_id"=>"6507230497"}, {"first_name"=>"Yaniv", "last_name"=>"Dotan", "scopus_author_id"=>"56838056800"}, {"first_name"=>"Amit", "last_name"=>"Hochberg", "scopus_author_id"=>"56094692000"}, {"first_name"=>"Yoram", "last_name"=>"Reiter", "scopus_author_id"=>"7003279893"}, {"first_name"=>"Moti", "last_name"=>"Grupper", "scopus_author_id"=>"22937557100"}, {"first_name"=>"Isaac", "last_name"=>"Srugo", "scopus_author_id"=>"7003330038"}, {"first_name"=>"Paul", "last_name"=>"Feigin", "scopus_author_id"=>"6701896813"}, {"first_name"=>"Malka", "last_name"=>"Gorfine", "scopus_author_id"=>"6603896361"}, {"first_name"=>"Irina", "last_name"=>"Chistyakov", "scopus_author_id"=>"8951706500"}, {"first_name"=>"Ron", "last_name"=>"Dagan", "scopus_author_id"=>"57189091284"}, {"first_name"=>"Adi", "last_name"=>"Klein", "scopus_author_id"=>"56567254800"}, {"first_name"=>"Israel", "last_name"=>"Potasman", "scopus_author_id"=>"7006423283"}, {"first_name"=>"Eran", "last_name"=>"Eden", "scopus_author_id"=>"15834314500"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"603271558", "sgr"=>"84956638767", "pmid"=>"25785720", "scopus"=>"2-s2.0-84956638767", "isbn"=>"1932-6203", "doi"=>"10.1371/journal.pone.0120012", "issn"=>"19326203"}, "id"=>"87b94e94-fc92-3061-b2b8-33826867fc43", "abstract"=>"Bacterial and viral infections are often clinically indistinguishable, leading to inappropriate patient management and antibiotic misuse. Bacterial-induced host proteins such as procalcitonin, C-reactive protein (CRP), and Interleukin-6, are routinely used to support diagnosis of infection. However, their performance is negatively affected by inter-patient variability, including time from symptom onset, clinical syndrome, and pathogens. Our aim was to identify novel viral-induced host proteins that can complement bacterial-induced proteins to increase diagnostic accuracy. Initially, we conducted a bioinformatic screen to identify putative circulating host immune response proteins. The resulting 600 candidates were then quantitatively screened for diagnostic potential using blood samples from 1002 prospectively recruited patients with suspected acute infectious disease and controls with no apparent infection. For each patient, three independent physicians assigned a diagnosis based on comprehensive clinical and laboratory investigation including PCR for 21 pathogens yielding 319 bacterial, 334 viral, 112 control and 98 indeterminate diagnoses; 139 patients were excluded based on predetermined criteria. The best performing host-protein was TNF-related apoptosis-inducing ligand (TRAIL) (area under the curve [AUC] of 0.89; 95% confidence interval [CI], 0.86 to 0.91), which was consistently up-regulated in viral infected patients. We further developed a multi-protein signature using logistic-regression on half of the patients and validated it on the remaining half. The signature with the highest precision included both viral- and bacterial-induced proteins: TRAIL, Interferon gamma-induced protein-10, and CRP (AUC of 0.94; 95% CI, 0.92 to 0.96). The signature was superior to any of the individual proteins (P<0.001), as well as routinely used clinical parameters and their combinations (P<0.001). It remained robust across different physiological systems, times from symptom onset, and pathogens (AUCs 0.87-1.0). The accurate differential diagnosis provided by this novel combination of viral- and bacterial-induced proteins has the potential to improve management of patients with acute infections and reduce antibiotic misuse.", "link"=>"http://www.mendeley.com/research/novel-hostproteome-signature-distinguishing-between-acute-bacterial-viral-infections", "reader_count"=>117, "reader_count_by_academic_status"=>{"Unspecified"=>8, "Professor > Associate Professor"=>6, "Librarian"=>1, "Researcher"=>33, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>19, "Student > Postgraduate"=>8, "Other"=>9, "Student > Master"=>16, "Student > Bachelor"=>8, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>5}, "reader_count_by_user_role"=>{"Unspecified"=>8, "Professor > Associate Professor"=>6, "Librarian"=>1, "Researcher"=>33, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>19, "Student > Postgraduate"=>8, "Other"=>9, "Student > Master"=>16, "Student > Bachelor"=>8, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>11, "Agricultural and Biological Sciences"=>33, "Arts and Humanities"=>1, "Chemical Engineering"=>1, "Computer Science"=>5, "Economics, Econometrics and Finance"=>1, "Engineering"=>7, "Biochemistry, Genetics and Molecular Biology"=>10, "Mathematics"=>2, "Medicine and Dentistry"=>37, "Pharmacology, Toxicology and Pharmaceutical Science"=>4, "Physics and Astronomy"=>1, "Social Sciences"=>1, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>37}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Mathematics"=>{"Mathematics"=>2}, "Unspecified"=>{"Unspecified"=>11}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>4}, "Chemical Engineering"=>{"Chemical Engineering"=>1}, "Arts and Humanities"=>{"Arts and Humanities"=>1}, "Engineering"=>{"Engineering"=>7}, "Economics, Econometrics and Finance"=>{"Economics, Econometrics and Finance"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>33}, "Computer Science"=>{"Computer Science"=>5}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>10}}, "reader_count_by_country"=>{"United States"=>2, "Taiwan"=>1, "Brazil"=>2, "United Kingdom"=>2, "South Africa"=>1, "Switzerland"=>1}, "group_count"=>11}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84956638767"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84956638767?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84956638767&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84956638767&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84956638767", "dc:identifier"=>"SCOPUS_ID:84956638767", "eid"=>"2-s2.0-84956638767", "dc:title"=>"A novel host-proteome signature for distinguishing between acute bacterial and viral infections", "dc:creator"=>"Oved K.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-03-18", "prism:coverDisplayDate"=>"18 March 2015", "prism:doi"=>"10.1371/journal.pone.0120012", "citedby-count"=>"47", "pubmed-id"=>"25785720", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0120012", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Article Coverage

Article Coverage Curated

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0120012", "share_count"=>21, "like_count"=>100, "comment_count"=>13, "click_count"=>0, "total_count"=>134}

Journal Comments | Further Information

Twitter

Counter

  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"831", "xml_views"=>"3", "html_views"=>"5153"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"220", "xml_views"=>"2", "html_views"=>"1728"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"92", "xml_views"=>"0", "html_views"=>"571"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"76", "xml_views"=>"0", "html_views"=>"651"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"59", "xml_views"=>"1", "html_views"=>"454"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"52", "xml_views"=>"0", "html_views"=>"483"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"66", "xml_views"=>"1", "html_views"=>"357"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"41", "xml_views"=>"0", "html_views"=>"277"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"54", "xml_views"=>"1", "html_views"=>"294"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"70", "xml_views"=>"0", "html_views"=>"340"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"66", "xml_views"=>"0", "html_views"=>"452"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"366"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"47", "xml_views"=>"0", "html_views"=>"222"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"94", "xml_views"=>"0", "html_views"=>"307"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"78", "xml_views"=>"0", "html_views"=>"280"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"59", "xml_views"=>"0", "html_views"=>"239"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"50", "xml_views"=>"0", "html_views"=>"288"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"34", "xml_views"=>"0", "html_views"=>"153"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"58", "xml_views"=>"0", "html_views"=>"243"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"138", "xml_views"=>"0", "html_views"=>"505"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"42", "xml_views"=>"0", "html_views"=>"400"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"90", "xml_views"=>"2", "html_views"=>"498"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"60", "xml_views"=>"2", "html_views"=>"365"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"86", "xml_views"=>"4", "html_views"=>"349"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"79", "xml_views"=>"2", "html_views"=>"453"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"66", "xml_views"=>"1", "html_views"=>"402"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"105", "xml_views"=>"1", "html_views"=>"291"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"78", "xml_views"=>"2", "html_views"=>"303"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"55", "xml_views"=>"2", "html_views"=>"236"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"48", "xml_views"=>"1", "html_views"=>"214"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"67", "xml_views"=>"2", "html_views"=>"396"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"94", "xml_views"=>"2", "html_views"=>"309"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"51", "xml_views"=>"1", "html_views"=>"450"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"54", "xml_views"=>"1", "html_views"=>"466"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"81", "xml_views"=>"1", "html_views"=>"495"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"79", "xml_views"=>"0", "html_views"=>"259"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"55", "xml_views"=>"2", "html_views"=>"257"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"74", "xml_views"=>"2", "html_views"=>"256"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"79", "xml_views"=>"2", "html_views"=>"258"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"47", "xml_views"=>"3", "html_views"=>"189"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"56", "xml_views"=>"5", "html_views"=>"215"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"65", "xml_views"=>"3", "html_views"=>"223"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"63", "xml_views"=>"0", "html_views"=>"230"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"81", "xml_views"=>"5", "html_views"=>"174"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"107", "xml_views"=>"4", "html_views"=>"158"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"67", "xml_views"=>"1", "html_views"=>"125"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"71", "xml_views"=>"1", "html_views"=>"179"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"52", "xml_views"=>"0", "html_views"=>"150"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"62", "xml_views"=>"0", "html_views"=>"159"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"58", "xml_views"=>"1", "html_views"=>"158"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"67", "xml_views"=>"1", "html_views"=>"147"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"43", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"43", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"34"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1958402"], "description"=>"<p>(A) An overview of the study workflow. n<sub>Bacterial</sub>, n<sub>Viral</sub> and n<sub>Control</sub> represent the number of bacterial (including mixed bacterial plus viral co-infections), viral and control (with no apparent infectious disease) cases, respectively. CBC—complete blood count. (B) An overview of protein screening, model construction and validation process.</p>", "links"=>[], "tags"=>["crp", "pcr", "ci", "antibiotic misuse", "infection", "auc", "symptom onset", "protein"], "article_id"=>1341152, "categories"=>["Biological Sciences"], "users"=>["Kfir Oved", "Asi Cohen", "Olga Boico", "Roy Navon", "Tom Friedman", "Liat Etshtein", "Or Kriger", "Ellen Bamberger", "Yura Fonar", "Renata Yacobov", "Ron Wolchinsky", "Galit Denkberg", "Yaniv Dotan", "Amit Hochberg", "Yoram Reiter", "Moti Grupper", "Isaac Srugo", "Paul Feigin", "Malka Gorfine", "Irina Chistyakov", "Ron Dagan", "Adi Klein", "Israel Potasman", "Eran Eden"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0120012.g001", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Study_workflow_/1341152", "title"=>"Study workflow.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-18 04:22:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/1958411", "https://ndownloader.figshare.com/files/1958412", "https://ndownloader.figshare.com/files/1958413", "https://ndownloader.figshare.com/files/1958414", "https://ndownloader.figshare.com/files/1958415", "https://ndownloader.figshare.com/files/1958416"], "description"=>"<div><p>Bacterial and viral infections are often clinically indistinguishable, leading to inappropriate patient management and antibiotic misuse. Bacterial-induced host proteins such as procalcitonin, C-reactive protein (CRP), and Interleukin-6, are routinely used to support diagnosis of infection. However, their performance is negatively affected by inter-patient variability, including time from symptom onset, clinical syndrome, and pathogens. Our aim was to identify novel viral-induced host proteins that can complement bacterial-induced proteins to increase diagnostic accuracy. Initially, we conducted a bioinformatic screen to identify putative circulating host immune response proteins. The resulting 600 candidates were then quantitatively screened for diagnostic potential using blood samples from 1002 prospectively recruited patients with suspected acute infectious disease and controls with no apparent infection. For each patient, three independent physicians assigned a diagnosis based on comprehensive clinical and laboratory investigation including PCR for 21 pathogens yielding 319 bacterial, 334 viral, 112 control and 98 indeterminate diagnoses; 139 patients were excluded based on predetermined criteria. The best performing host-protein was TNF-related apoptosis-inducing ligand (TRAIL) (area under the curve [AUC] of 0.89; 95% confidence interval [CI], 0.86 to 0.91), which was consistently up-regulated in viral infected patients. We further developed a multi-protein signature using logistic-regression on half of the patients and validated it on the remaining half. The signature with the highest precision included both viral- and bacterial-induced proteins: TRAIL, Interferon gamma-induced protein-10, and CRP (AUC of 0.94; 95% CI, 0.92 to 0.96). The signature was superior to any of the individual proteins (P<0.001), as well as routinely used clinical parameters and their combinations (P<0.001). It remained robust across different physiological systems, times from symptom onset, and pathogens (AUCs 0.87-1.0). The accurate differential diagnosis provided by this novel combination of viral- and bacterial-induced proteins has the potential to improve management of patients with acute infections and reduce antibiotic misuse.</p></div>", "links"=>[], "tags"=>["crp", "pcr", "ci", "antibiotic misuse", "infection", "auc", "symptom onset", "protein"], "article_id"=>1341161, "categories"=>["Biological Sciences"], "users"=>["Kfir Oved", "Asi Cohen", "Olga Boico", "Roy Navon", "Tom Friedman", "Liat Etshtein", "Or Kriger", "Ellen Bamberger", "Yura Fonar", "Renata Yacobov", "Ron Wolchinsky", "Galit Denkberg", "Yaniv Dotan", "Amit Hochberg", "Yoram Reiter", "Moti Grupper", "Isaac Srugo", "Paul Feigin", "Malka Gorfine", "Irina Chistyakov", "Ron Dagan", "Adi Klein", "Israel Potasman", "Eran Eden"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0120012.s001", "https://dx.doi.org/10.1371/journal.pone.0120012.s002", "https://dx.doi.org/10.1371/journal.pone.0120012.s003", "https://dx.doi.org/10.1371/journal.pone.0120012.s004", "https://dx.doi.org/10.1371/journal.pone.0120012.s005", "https://dx.doi.org/10.1371/journal.pone.0120012.s006"], "stats"=>{"downloads"=>20, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_Novel_Host_Proteome_Signature_for_Distinguishing_between_Acute_Bacterial_and_Viral_Infections_/1341161", "title"=>"A Novel Host-Proteome Signature for Distinguishing between Acute Bacterial and Viral Infections", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-03-18 04:22:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/1958409"], "description"=>"<p>Values are presented as total numbers, followed by the corresponding percentages in brackets. Only microorganisms that were detected in more than five patients are presented. CNS- central nervous system, GI—gastroenteritis, LRTI—lower respiratory tract infection, UTRI—upper respiratory tract infection, UTI—urinary tract infection, N/A—healthy controls or patients in which data was not obtained. Influenza A subgroup included H1N1 strains. The atypical bacteria subgroup included <i>Chlamydophila pneumoniae</i>, <i>Mycoplasma pneumonia</i> and <i>Legionella pneumophila</i>. The Enteric viruses subgroup included Rota virus, Astrovirus, Enteric Adenovirus and Norovirus G I/II. In the clinical syndrome analysis the LRTI group included pneumonia, bronchiolitis, acute bronchitis, and laryngitis; the URTI group included pharyngitis, acute otitis media, acute sinusitis and acute tonsillitis.</p><p>Baseline characteristics of the study cohort patients.</p>", "links"=>[], "tags"=>["crp", "pcr", "ci", "antibiotic misuse", "infection", "auc", "symptom onset", "protein"], "article_id"=>1341159, "categories"=>["Biological Sciences"], "users"=>["Kfir Oved", "Asi Cohen", "Olga Boico", "Roy Navon", "Tom Friedman", "Liat Etshtein", "Or Kriger", "Ellen Bamberger", "Yura Fonar", "Renata Yacobov", "Ron Wolchinsky", "Galit Denkberg", "Yaniv Dotan", "Amit Hochberg", "Yoram Reiter", "Moti Grupper", "Isaac Srugo", "Paul Feigin", "Malka Gorfine", "Irina Chistyakov", "Ron Dagan", "Adi Klein", "Israel Potasman", "Eran Eden"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0120012.t001", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Baseline_characteristics_of_the_study_cohort_patients_/1341159", "title"=>"Baseline characteristics of the study cohort patients.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-03-18 04:22:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/1958410"], "description"=>"<p>Left: Performance estimates and their 95% CIs were obtained using a leave-10%-out cross-validation on all patients in the study cohort (n<sub>Bacterial</sub> = 319, n<sub>Viral</sub> = 334), Unanimous sub-cohort (n<sub>Bacterial</sub> = 256, n<sub>Viral</sub> = 271), and Microbiologically confirmed sub-cohort (n<sub>Bacterial</sub> = 68, n<sub>Viral</sub> = 173). Right: The analysis was repeated after filtering out patients with an equivocal immune response (study cohort [n<sub>Bacterial</sub> = 290, n<sub>Viral</sub> = 277, n<sub>equivocal</sub> = 86], Unanimous [n<sub>Bacterial</sub> = 233, n<sub>Viral</sub> = 232, n<sub>equivocal</sub> = 62] and Microbiologically confirmed [n<sub>Bacterial</sub> = 64, n<sub>Viral</sub> = 160, n<sub>equivocal</sub> = 17]), which resembles the way clinicians are likely to use the signature. Additional measures of accuracy, including positive predictive value and negative predictive value, and their dependency on bacterial prevalence are described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120012#pone.0120012.s005\" target=\"_blank\">S5 Data</a>.</p><p>Signature measures of accuracy for diagnosing bacterial vs viral infections.</p>", "links"=>[], "tags"=>["crp", "pcr", "ci", "antibiotic misuse", "infection", "auc", "symptom onset", "protein"], "article_id"=>1341160, "categories"=>["Biological Sciences"], "users"=>["Kfir Oved", "Asi Cohen", "Olga Boico", "Roy Navon", "Tom Friedman", "Liat Etshtein", "Or Kriger", "Ellen Bamberger", "Yura Fonar", "Renata Yacobov", "Ron Wolchinsky", "Galit Denkberg", "Yaniv Dotan", "Amit Hochberg", "Yoram Reiter", "Moti Grupper", "Isaac Srugo", "Paul Feigin", "Malka Gorfine", "Irina Chistyakov", "Ron Dagan", "Adi Klein", "Israel Potasman", "Eran Eden"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0120012.t002", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Signature_measures_of_accuracy_for_diagnosing_bacterial_vs_viral_infections_/1341160", "title"=>"Signature measures of accuracy for diagnosing bacterial vs viral infections.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-03-18 04:22:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/1958408"], "description"=>"<p>PAMPs—pathogen-associated molecular patterns; PGN—peptidoglycan; LPS—lipopolysaccharide.</p>", "links"=>[], "tags"=>["crp", "pcr", "ci", "antibiotic misuse", "infection", "auc", "symptom onset", "protein"], "article_id"=>1341158, "categories"=>["Biological Sciences"], "users"=>["Kfir Oved", "Asi Cohen", "Olga Boico", "Roy Navon", "Tom Friedman", "Liat Etshtein", "Or Kriger", "Ellen Bamberger", "Yura Fonar", "Renata Yacobov", "Ron Wolchinsky", "Galit Denkberg", "Yaniv Dotan", "Amit Hochberg", "Yoram Reiter", "Moti Grupper", "Isaac Srugo", "Paul Feigin", "Malka Gorfine", "Irina Chistyakov", "Ron Dagan", "Adi Klein", "Israel Potasman", "Eran Eden"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0120012.g004", "stats"=>{"downloads"=>8, "page_views"=>269, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TRAIL_IP_10_and_CRP_participate_in_different_signaling_pathways_and_exhibit_complementary_dynamics_in_response_to_bacterial_B_and_viral_V_infections_/1341158", "title"=>"TRAIL, IP-10 and CRP participate in different signaling pathways and exhibit complementary dynamics in response to bacterial (B) and viral (V) infections.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-18 04:22:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/1958407"], "description"=>"<p>(A) Signature AUCs in subgroups of the study cohort (bacterial and viral) are depicted. Square size is proportional to number of patients and error bars represent 95% CI. In the Pathogens analysis, each virus was compared to bacteria affecting the same physiological system, indicated in brackets. R-respiratory, C-central nervous system, G-gastrointestinal, U-urinary, K-skin, S-systemic (i.e. non-localized). Only pathogens detected in more than 5 patients are presented. PED—pediatric emergency departments, ED—emergency departments. For subgroup definitions see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120012#pone.0120012.t001\" target=\"_blank\">Table 1</a> legend. (B) Performance of clinical and lab parameters as well as the best performing pair (ANC and Lym %), triplet (ANC, Lym % and Pulse), and quadruplets (ANC, Lym %, Pulse, Mono %) of parameters, the values of which were combined using a logistic regression. Comparison was done on the entire study cohort (n = 653), apart from pulse (recorded in 292 bacterial and 326 viral patients), and respiratory rate (recorded in 292 bacterial and 326 viral patients). The signature performed significantly better (<i>P</i><10<sup>–15</sup>) than the optimal quadruplet. (C) The signature performed significantly better (<i>P</i><10<sup>–8</sup>) than biomarkers with a well-established role in the host response to infections. For each of the select biomarkers, analysis was performed in a subgroup of the study cohort (43≤n≤154 for each analysis, a convenience sample, n depended on the strength of the signal). Error bars represent 95% CI.</p>", "links"=>[], "tags"=>["crp", "pcr", "ci", "antibiotic misuse", "infection", "auc", "symptom onset", "protein"], "article_id"=>1341157, "categories"=>["Biological Sciences"], "users"=>["Kfir Oved", "Asi Cohen", "Olga Boico", "Roy Navon", "Tom Friedman", "Liat Etshtein", "Or Kriger", "Ellen Bamberger", "Yura Fonar", "Renata Yacobov", "Ron Wolchinsky", "Galit Denkberg", "Yaniv Dotan", "Amit Hochberg", "Yoram Reiter", "Moti Grupper", "Isaac Srugo", "Paul Feigin", "Malka Gorfine", "Irina Chistyakov", "Ron Dagan", "Adi Klein", "Israel Potasman", "Eran Eden"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0120012.g003", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Signature_performance_is_robust_across_different_patient_subgroups_and_outperforms_lab_parameters_and_protein_biomarkers_/1341157", "title"=>"Signature performance is robust across different patient subgroups and outperforms lab parameters and protein biomarkers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-18 04:22:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/1958406"], "description"=>"<p>Box plots for TRAIL (A), IP-10 (B), and CRP (C), measured over the entire study cohort (n = 765) are presented. Red line and circle correspond to group median and average respectively; t-test p-values between bacterial and viral groups and between infectious (bacterial and viral) vs non-infectious (including healthy subjects) are depicted.</p>", "links"=>[], "tags"=>["crp", "pcr", "ci", "antibiotic misuse", "infection", "auc", "symptom onset", "protein"], "article_id"=>1341156, "categories"=>["Biological Sciences"], "users"=>["Kfir Oved", "Asi Cohen", "Olga Boico", "Roy Navon", "Tom Friedman", "Liat Etshtein", "Or Kriger", "Ellen Bamberger", "Yura Fonar", "Renata Yacobov", "Ron Wolchinsky", "Galit Denkberg", "Yaniv Dotan", "Amit Hochberg", "Yoram Reiter", "Moti Grupper", "Isaac Srugo", "Paul Feigin", "Malka Gorfine", "Irina Chistyakov", "Ron Dagan", "Adi Klein", "Israel Potasman", "Eran Eden"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0120012.g002", "stats"=>{"downloads"=>6, "page_views"=>114, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_proteins_TRAIL_IP_10_and_CRP_are_differentially_expressed_in_bacterial_viral_and_non_infectious_patients_/1341156", "title"=>"The proteins TRAIL, IP-10 and CRP are differentially expressed in bacterial, viral and non-infectious patients.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-18 04:22:06"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"80", "full-text"=>"96", "pdf"=>"35", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"40", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"51", "full-text"=>"59", "pdf"=>"44", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"33", "full-text"=>"38", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"35", "full-text"=>"40", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"46", "full-text"=>"52", "pdf"=>"41", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"23", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"38", "full-text"=>"41", "pdf"=>"25", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"35", "full-text"=>"30", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"37", "full-text"=>"32", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"6", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"35", "full-text"=>"32", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"18", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"31", "full-text"=>"31", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"28", "full-text"=>"25", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"52", "full-text"=>"36", "pdf"=>"21", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"27", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"38", "full-text"=>"59", "pdf"=>"38", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"23", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"57", "full-text"=>"58", "pdf"=>"23", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"4", "cited-by"=>"1", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"44", "full-text"=>"31", "pdf"=>"23", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"24", "full-text"=>"195", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"28", "full-text"=>"27", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"42", "full-text"=>"40", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"27", "supp-data"=>"5", "cited-by"=>"1", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"36", "full-text"=>"35", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"23", "full-text"=>"27", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"26", "full-text"=>"29", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"43", "full-text"=>"49", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"9", "cited-by"=>"1", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"43", "full-text"=>"45", "pdf"=>"21", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"25", "full-text"=>"24", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"26", "full-text"=>"29", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"34", "full-text"=>"40", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"36", "full-text"=>"39", "pdf"=>"24", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"33", "full-text"=>"29", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"12", "cited-by"=>"1", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"19", "full-text"=>"24", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"21", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"32", "full-text"=>"35", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"21", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"4", "cited-by"=>"1", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"24", "full-text"=>"27", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"33", "full-text"=>"33", "pdf"=>"11", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"21", "full-text"=>"25", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"22", "full-text"=>"26", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"33", "full-text"=>"34", "pdf"=>"11", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"30", "full-text"=>"25", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"22", "full-text"=>"18", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"36", "full-text"=>"29", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"18", "supp-data"=>"17", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"21", "full-text"=>"27", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"25", "full-text"=>"27", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"22", "full-text"=>"27", "pdf"=>"12", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"36", "full-text"=>"35", "pdf"=>"12", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts