Infection Dynamic of Symbiotic Bacteria in the Pea Aphid Acyrthosiphon pisum Gut and Host Immune Response at the Early Steps in the Infection Process
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{"title"=>"Infection dynamic of symbiotic bacteria in the pea aphid Acyrthosiphon pisum gut and host immune response at the early steps in the infection process", "type"=>"journal", "authors"=>[{"first_name"=>"François", "last_name"=>"Renoz", "scopus_author_id"=>"56582554500"}, {"first_name"=>"Christine", "last_name"=>"Noël", "scopus_author_id"=>"56411711800"}, {"first_name"=>"Abdelmounaim", "last_name"=>"Errachid", "scopus_author_id"=>"26427619300"}, {"first_name"=>"Vincent", "last_name"=>"Foray", "scopus_author_id"=>"41561320700"}, {"first_name"=>"Thierry", "last_name"=>"Hance", "scopus_author_id"=>"7003476073"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"25811863", "doi"=>"10.1371/journal.pone.0122099", "sgr"=>"84926318672", "isbn"=>"1932-6203 (Electronic) 1932-6203 (Linking)", "scopus"=>"2-s2.0-84926318672", "issn"=>"19326203", "pui"=>"603466584"}, "id"=>"b6159b27-a43a-33ee-b2df-e0c3c6a96575", "abstract"=>"In addition to its obligatory symbiont Buchnera aphidicola, the pea aphid Acyrthosiphon pisum can harbor several facultative bacterial symbionts which can be mutualistic in the context of various ecological interactions. Belonging to a genus where many members have been described as pathogen in invertebrates, Serratia symbiotica is one of the most common facultative partners found in aphids. The recent discovery of strains able to grow outside their host allowed us to simulate environmental acquisition of symbiotic bacteria by aphids. Here, we performed an experiment to characterize the A. pisum response to the ingestion of the free-living S. symbiotica CWBI-2.3T in comparison to the ingestion of the pathogenic Serratia marcescens Db11 at the early steps in the infection process. We found that, while S. marcescens Db11 killed the aphids within a few days, S. symbiotica CWBI-2.3T did not affect host survival and colonized the whole digestive tract within a few days. Gene expression analysis of immune genes suggests that S. symbiotica CWBI-2.3T did not trigger an immune reaction, while S. marcescens Db11 did, and supports the hypothesis of a fine-tuning of the host immune response set-up for fighting pathogens while maintaining mutualistic partners. Our results also suggest that the lysosomal system and the JNK pathway are possibly involved in the regulation of invasive bacteria in aphids and that the activation of the JNK pathway is IMD-independent in the pea aphid.", "link"=>"http://www.mendeley.com/research/infection-dynamic-symbiotic-bacteria-pea-aphid-acyrthosiphon-pisum-gut-host-immune-response-early-st-4", "reader_count"=>43, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>6, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>19, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>2, "Student > Bachelor"=>5, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>6, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>19, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>2, "Student > Bachelor"=>5, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Environmental Science"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>33, "Medicine and Dentistry"=>1, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>33}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>2}, "Environmental Science"=>{"Environmental Science"=>2}}, "reader_count_by_country"=>{"United States"=>2, "Spain"=>1}, "group_count"=>2}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1990962"], "description"=>"<p>Red Cy3 signals are <i>S</i>. <i>symbiotica</i> CWBI-2.3<sup>T</sup>, Green Cy5 signals are <i>B</i>. <i>aphidicola</i> and blue SYTOX Green signals are host tissues. (A) Red fluorescence in the midgut indicates ingestion of <i>S</i>. <i>symbiotica</i> CWBI-2.3<sup>T</sup> after feeding of contaminated diet. (B and C) 5 days and 10 days post-challenge, red fluorescence is visible in the midgut as well as in the entire intestine.</p>", "links"=>[], "tags"=>["marcescens Db 11", "Host Immune Response", "Pea Aphid Acyrthosiphon pisum Gut", "symbiont Buchnera aphidicola", "symbiotica", "gene expression analysis", "Serratia marcescens Db 11", "pea aphid Acyrthosiphon pisum", "host", "JNK pathway", "CWBI"], "article_id"=>1357712, "categories"=>["Biological Sciences"], "users"=>["François Renoz", "Christine Noël", "Abdelmounaim Errachid", "Vincent Foray", "Thierry Hance"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122099.g002", "stats"=>{"downloads"=>1, "page_views"=>31, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Whole_mount_fluorescence_in_situ_hybridization_of_S_symbiotica_infected_A_pisum_0_day_post_ingestion_A_5_days_post_ingestion_B_and_10_days_post_ingestion_C_/1357712", "title"=>"Whole mount fluorescence <i>in situ</i> hybridization of <i>S</i>. <i>symbiotica</i>-infected <i>A</i>. <i>pisum</i> 0 day post-ingestion (A), 5 days post-ingestion (B) and 10 days post-ingestion (C).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-26 04:47:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/1990960"], "description"=>"<p>Graph show the survival of <i>A</i>. <i>pisum</i> aphids infected with <i>S</i>. <i>marcescens</i> Db11 (red), <i>S</i>. <i>symbiotica</i> CWBI-2.3<sup>T</sup> (green) and uninfected aphids (blue). Error bars represent ± 95% CI.</p>", "links"=>[], "tags"=>["marcescens Db 11", "Host Immune Response", "Pea Aphid Acyrthosiphon pisum Gut", "symbiont Buchnera aphidicola", "symbiotica", "gene expression analysis", "Serratia marcescens Db 11", "pea aphid Acyrthosiphon pisum", "host", "JNK pathway", "CWBI"], "article_id"=>1357710, "categories"=>["Biological Sciences"], "users"=>["François Renoz", "Christine Noël", "Abdelmounaim Errachid", "Vincent Foray", "Thierry Hance"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122099.g001", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Survival_of_A_pisum_aphids_after_bacterial_ingestion_/1357710", "title"=>"Survival of <i>A</i>. <i>pisum</i> aphids after bacterial ingestion.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-26 04:47:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/1990965"], "description"=>"<p>List of genes and primers used in the study for quantitative PCR analyzed by quantitative real-time RT-PCR analysis.</p>", "links"=>[], "tags"=>["marcescens Db 11", "Host Immune Response", "Pea Aphid Acyrthosiphon pisum Gut", "symbiont Buchnera aphidicola", "symbiotica", "gene expression analysis", "Serratia marcescens Db 11", "pea aphid Acyrthosiphon pisum", "host", "JNK pathway", "CWBI"], "article_id"=>1357715, "categories"=>["Biological Sciences"], "users"=>["François Renoz", "Christine Noël", "Abdelmounaim Errachid", "Vincent Foray", "Thierry Hance"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122099.t001", "stats"=>{"downloads"=>3, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_List_of_genes_and_primers_used_in_the_study_for_quantitative_PCR_analyzed_by_quantitative_real_time_RT_PCR_analysis_/1357715", "title"=>"List of genes and primers used in the study for quantitative PCR analyzed by quantitative real-time RT-PCR analysis.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-03-26 04:47:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/1990963"], "description"=>"<p><i>S</i>. <i>symbiotica</i> CWBI-2.3<sup>T</sup> densities are expressed in terms of <i>DnaK</i> gene copies per <i>ef1-α</i> gene copy. Points and bars indicate means and standard errors. Points sharing the same letters are not significantly different (p > 0.05) using post-hoc Tukey’s tests.</p>", "links"=>[], "tags"=>["marcescens Db 11", "Host Immune Response", "Pea Aphid Acyrthosiphon pisum Gut", "symbiont Buchnera aphidicola", "symbiotica", "gene expression analysis", "Serratia marcescens Db 11", "pea aphid Acyrthosiphon pisum", "host", "JNK pathway", "CWBI"], "article_id"=>1357713, "categories"=>["Biological Sciences"], "users"=>["François Renoz", "Christine Noël", "Abdelmounaim Errachid", "Vincent Foray", "Thierry Hance"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122099.g003", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Infection_dynamic_of_S_symbiotica_CWBI_2_3_T_during_a_period_of_ten_days_post_challenge_/1357713", "title"=>"Infection dynamic of <i>S</i>. <i>symbiotica</i> CWBI-2.3<sup>T</sup> during a period of ten days post-challenge.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-26 04:47:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/1990964"], "description"=>"<p>The mRNA levels of selected genes in infection with <i>S</i>. <i>symbiotica</i> CWBI-2.1<sup>T</sup> (green), <i>S</i>. <i>marcescens</i> Db11 (red) were determined and are shown relative to their expression levels in untreated insects (blue). The expression level was normalized by comparative <i>C</i><sub><i>T</i></sub> (ΔΔ<i>C</i><sub><i>T</i></sub>) method using <i>ef1-α</i> as reference gene. Columns and bars indicate means and standard errors.</p>", "links"=>[], "tags"=>["marcescens Db 11", "Host Immune Response", "Pea Aphid Acyrthosiphon pisum Gut", "symbiont Buchnera aphidicola", "symbiotica", "gene expression analysis", "Serratia marcescens Db 11", "pea aphid Acyrthosiphon pisum", "host", "JNK pathway", "CWBI"], "article_id"=>1357714, "categories"=>["Biological Sciences"], "users"=>["François Renoz", "Christine Noël", "Abdelmounaim Errachid", "Vincent Foray", "Thierry Hance"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122099.g004", "stats"=>{"downloads"=>2, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Quantitative_real_time_RT_PCR_analysis_of_transcriptional_levels_of_six_selected_A_pisum_genes_in_response_to_bacterial_infection_/1357714", "title"=>"Quantitative real-time RT-PCR analysis of transcriptional levels of six selected <i>A</i>. <i>pisum</i> genes in response to bacterial infection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-03-26 04:47:44"}

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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