Immunophenotyping of Waldenströms Macroglobulinemia Cell Lines Reveals Distinct Patterns of Surface Antigen Expression: Potential Biological and Therapeutic Implications
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Immunophenotyping of Waldenströms macroglobulinemia cell lines reveals distinct patterns of surface antigen expression: Potential biological and therapeutic implications", "type"=>"journal", "authors"=>[{"first_name"=>"Aneel", "last_name"=>"Paulus", "scopus_author_id"=>"37038178500"}, {"first_name"=>"Kasyapa S.", "last_name"=>"Chitta", "scopus_author_id"=>"6506769347"}, {"first_name"=>"Paul K.", "last_name"=>"Wallace", "scopus_author_id"=>"17636964500"}, {"first_name"=>"Pooja P.", "last_name"=>"Advani", "scopus_author_id"=>"37060382800"}, {"first_name"=>"Sharoon", "last_name"=>"Akhtar", "scopus_author_id"=>"55929113500"}, {"first_name"=>"Maja", "last_name"=>"Kuranz-Blake", "scopus_author_id"=>"56648131300"}, {"first_name"=>"Sikander", "last_name"=>"Ailawadhi", "scopus_author_id"=>"12779357400"}, {"first_name"=>"Asher A.", "last_name"=>"Chanan-Khan", "scopus_author_id"=>"6701710137"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"604108702", "sgr"=>"84929492246", "pmid"=>"25853860", "scopus"=>"2-s2.0-84929492246", "isbn"=>"1932-6203 (Electronic) 1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0122338", "issn"=>"19326203"}, "id"=>"5404cdad-76a5-3937-b623-06988667b0c2", "abstract"=>"Waldenströms macroglobulinemia (WM) is a subtype of Non-Hodgkin's lymphoma in which the tumor cell population is markedly heterogeneous, consisting of immunoglobulin-M secreting B-lymphocytes, plasmacytoid lymphocytes and plasma cells. Due to rarity of disease and scarcity of reliable preclinical models, many facets of WM molecular and phenotypic architecture remain incompletely understood. Currently, there are 3 human WM cell lines that are routinely used in experimental studies, namely, BCWM.1, MWCL-1 and RPCI-WM1. During establishment of RPCI-WM1, we observed loss of the CD19 and CD20 antigens, which are typically present on WM cells. Intrigued by this observation and in an effort to better define the immunophenotypic makeup of this cell line, we conducted a more comprehensive analysis for the presence or absence of other cell surface antigens that are present on the RPCI-WM1 model, as well as those on the two other WM cell lines, BCWM.1 and MWCL-1. We examined expression of 65 extracellular and 4 intracellular antigens, comprising B-cell, plasma cell, T-cell, NK-cell, myeloid and hematopoietic stem cell surface markers by flow cytometry analysis. RPCI-WM1 cells demonstrated decreased expression of CD19, CD20, and CD23 with enhanced expression of CD28, CD38 and CD184, antigens that were differentially expressed on BCWM.1 and MWCL-1 cells. Due to increased expression of CD184/CXCR4 and CD38, RPCI-WM1 represents a valuable model in which to study the effects anti-CXCR4 or anti-CD38 targeted therapies that are actively being developed for treatment of hematologic cancers. Overall, differences in surface antigen expression across the 3 cell lines may reflect the tumor clone population predominant in the index patients, from whom the cell lines were developed. Our analysis defines the utility of the most commonly employed WM cell lines as based on their immunophenotype profiles, highlighting unique differences that can be further studied for therapeutic exploit.", "link"=>"http://www.mendeley.com/research/immunophenotyping-waldenstr%C3%B6ms-macroglobulinemia-cell-lines-reveals-distinct-patterns-surface-antige", "reader_count"=>3, "reader_count_by_academic_status"=>{"Researcher"=>1, "Student > Postgraduate"=>1, "Student > Master"=>1}, "reader_count_by_user_role"=>{"Researcher"=>1, "Student > Postgraduate"=>1, "Student > Master"=>1}, "reader_count_by_subject_area"=>{"Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84929492246"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84929492246?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84929492246&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84929492246&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84929492246", "dc:identifier"=>"SCOPUS_ID:84929492246", "eid"=>"2-s2.0-84929492246", "dc:title"=>"Immunophenotyping of Waldenströms macroglobulinemia cell lines reveals distinct patterns of surface antigen expression: Potential biological and therapeutic implications", "dc:creator"=>"Paulus A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-04-08", "prism:coverDisplayDate"=>"8 April 2015", "prism:doi"=>"10.1371/journal.pone.0122338", "citedby-count"=>"6", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Mayo Clinic in Jacksonville, Florida", "affiliation-city"=>"Jacksonville", "affiliation-country"=>"United States"}], "pubmed-id"=>"25853860", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0122338", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0122338", "share_count"=>5, "like_count"=>0, "comment_count"=>4, "click_count"=>0, "total_count"=>9}

Counter

  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"83", "xml_views"=>"16", "html_views"=>"849"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"68"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"31"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"59"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"68"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"100"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"4", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"8"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2009874"], "description"=>"<p>For antigen detection, fluorescein (FITC), phycoerythrin (PE), phycoerythrin—cyanine 5 (PC5) or allophycocyanin (APC) conjugates of various antigen-specific antibodies were used. Flow cytometry shows the RPCI-WM1 cell line to be CD10, 11c, 19, 20, 23, 27, 40, 52 and 79b negative and CD22, 28, 38, 45, 45RO, 38, 138, κ light chain and intracellular-κ positive.</p>", "links"=>[], "tags"=>["BCWM .1", "MWCL", "4 intracellular antigens", "WM cell lines", "surface antigen expression", "tumor clone population", "cell surface markers", "cell surface antigens", "flow cytometry analysis", "CD 20 antigens", "tumor cell population", "3 cell lines"], "article_id"=>1371277, "categories"=>["Uncategorised"], "users"=>["Aneel Paulus", "Kasyapa S. Chitta", "Paul K. Wallace", "Pooja P. Advani", "Sharoon Akhtar", "Maja Kuranz-Blake", "Sikander Ailawadhi", "Asher A. Chanan-Khan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122338.g001", "stats"=>{"downloads"=>0, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_WM_specific_antigens_present_on_RPCI_WM1_/1371277", "title"=>"Expression of WM-specific antigens present on RPCI-WM1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-08 02:46:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/2009894"], "description"=>"<p>Comprehensive immunophenotype comparison of RPCI-WM1, MWCL-1 and BCWM.1.</p>", "links"=>[], "tags"=>["BCWM .1", "MWCL", "4 intracellular antigens", "WM cell lines", "surface antigen expression", "tumor clone population", "cell surface markers", "cell surface antigens", "flow cytometry analysis", "CD 20 antigens", "tumor cell population", "3 cell lines"], "article_id"=>1371294, "categories"=>["Uncategorised"], "users"=>["Aneel Paulus", "Kasyapa S. Chitta", "Paul K. Wallace", "Pooja P. Advani", "Sharoon Akhtar", "Maja Kuranz-Blake", "Sikander Ailawadhi", "Asher A. Chanan-Khan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122338.t004", "stats"=>{"downloads"=>4, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comprehensive_immunophenotype_comparison_of_RPCI_WM1_MWCL_1_and_BCWM_1_/1371294", "title"=>"Comprehensive immunophenotype comparison of RPCI-WM1, MWCL-1 and BCWM.1.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-04-08 02:46:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/2009888"], "description"=>"<p>Most widely expressed antigens on RPCI-WM1 cells.</p>", "links"=>[], "tags"=>["BCWM .1", "MWCL", "4 intracellular antigens", "WM cell lines", "surface antigen expression", "tumor clone population", "cell surface markers", "cell surface antigens", "flow cytometry analysis", "CD 20 antigens", "tumor cell population", "3 cell lines"], "article_id"=>1371291, "categories"=>["Uncategorised"], "users"=>["Aneel Paulus", "Kasyapa S. Chitta", "Paul K. Wallace", "Pooja P. Advani", "Sharoon Akhtar", "Maja Kuranz-Blake", "Sikander Ailawadhi", "Asher A. Chanan-Khan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122338.t002", "stats"=>{"downloads"=>2, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Most_widely_expressed_antigens_on_RPCI_WM1_cells_/1371291", "title"=>"Most widely expressed antigens on RPCI-WM1 cells.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-04-08 02:46:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/2009887"], "description"=>"<p>Expression of cell surface antigens on RPCI-WM1 model.</p>", "links"=>[], "tags"=>["BCWM .1", "MWCL", "4 intracellular antigens", "WM cell lines", "surface antigen expression", "tumor clone population", "cell surface markers", "cell surface antigens", "flow cytometry analysis", "CD 20 antigens", "tumor cell population", "3 cell lines"], "article_id"=>1371290, "categories"=>["Uncategorised"], "users"=>["Aneel Paulus", "Kasyapa S. Chitta", "Paul K. Wallace", "Pooja P. Advani", "Sharoon Akhtar", "Maja Kuranz-Blake", "Sikander Ailawadhi", "Asher A. Chanan-Khan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122338.t001", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_cell_surface_antigens_on_RPCI_WM1_model_/1371290", "title"=>"Expression of cell surface antigens on RPCI-WM1 model.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-04-08 02:46:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/2009898", "https://ndownloader.figshare.com/files/2009899", "https://ndownloader.figshare.com/files/2009900", "https://ndownloader.figshare.com/files/2009901"], "description"=>"<div><p>Waldenströms macroglobulinemia (WM) is a subtype of Non-Hodgkin’s lymphoma in which the tumor cell population is markedly heterogeneous, consisting of immunoglobulin-M secreting B-lymphocytes, plasmacytoid lymphocytes and plasma cells. Due to rarity of disease and scarcity of reliable preclinical models, many facets of WM molecular and phenotypic architecture remain incompletely understood. Currently, there are 3 human WM cell lines that are routinely used in experimental studies, namely, BCWM.1, MWCL-1 and RPCI-WM1. During establishment of RPCI-WM1, we observed loss of the CD19 and CD20 antigens, which are typically present on WM cells. Intrigued by this observation and in an effort to better define the immunophenotypic makeup of this cell line, we conducted a more comprehensive analysis for the presence or absence of other cell surface antigens that are present on the RPCI-WM1 model, as well as those on the two other WM cell lines, BCWM.1 and MWCL-1. We examined expression of 65 extracellular and 4 intracellular antigens, comprising B-cell, plasma cell, T-cell, NK-cell, myeloid and hematopoietic stem cell surface markers by flow cytometry analysis. RPCI-WM1 cells demonstrated decreased expression of CD19, CD20, and CD23 with enhanced expression of CD28, CD38 and CD184, antigens that were differentially expressed on BCWM.1 and MWCL-1 cells. Due to increased expression of CD184/CXCR4 and CD38, RPCI-WM1 represents a valuable model in which to study the effects anti-CXCR4 or anti-CD38 targeted therapies that are actively being developed for treatment of hematologic cancers. Overall, differences in surface antigen expression across the 3 cell lines may reflect the tumor clone population predominant in the index patients, from whom the cell lines were developed. Our analysis defines the utility of the most commonly employed WM cell lines as based on their immunophenotype profiles, highlighting unique differences that can be further studied for therapeutic exploit.</p></div>", "links"=>[], "tags"=>["BCWM .1", "MWCL", "4 intracellular antigens", "WM cell lines", "surface antigen expression", "tumor clone population", "cell surface markers", "cell surface antigens", "flow cytometry analysis", "CD 20 antigens", "tumor cell population", "3 cell lines"], "article_id"=>1371298, "categories"=>["Uncategorised"], "users"=>["Aneel Paulus", "Kasyapa S. Chitta", "Paul K. Wallace", "Pooja P. Advani", "Sharoon Akhtar", "Maja Kuranz-Blake", "Sikander Ailawadhi", "Asher A. Chanan-Khan"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0122338.s001", "https://dx.doi.org/10.1371/journal.pone.0122338.s002", "https://dx.doi.org/10.1371/journal.pone.0122338.s003", "https://dx.doi.org/10.1371/journal.pone.0122338.s004"], "stats"=>{"downloads"=>2, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunophenotyping_of_Waldenstr_246_ms_Macroglobulinemia_Cell_Lines_Reveals_Distinct_Patterns_of_Surface_Antigen_Expression_Potential_Biological_and_Therapeutic_Implications_/1371298", "title"=>"Immunophenotyping of Waldenströms Macroglobulinemia Cell Lines Reveals Distinct Patterns of Surface Antigen Expression: Potential Biological and Therapeutic Implications", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-04-08 02:46:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/2009890"], "description"=>"<p>Pattern of surface antigen expression (<u>></u>20%) across all 3 WM models.</p>", "links"=>[], "tags"=>["BCWM .1", "MWCL", "4 intracellular antigens", "WM cell lines", "surface antigen expression", "tumor clone population", "cell surface markers", "cell surface antigens", "flow cytometry analysis", "CD 20 antigens", "tumor cell population", "3 cell lines"], "article_id"=>1371292, "categories"=>["Uncategorised"], "users"=>["Aneel Paulus", "Kasyapa S. Chitta", "Paul K. Wallace", "Pooja P. Advani", "Sharoon Akhtar", "Maja Kuranz-Blake", "Sikander Ailawadhi", "Asher A. Chanan-Khan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122338.t003", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pattern_of_surface_antigen_expression_gt_20_across_all_3_WM_models_/1371292", "title"=>"Pattern of surface antigen expression (>20%) across all 3 WM models.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-04-08 02:46:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/2009886"], "description"=>"<p>A total of 8 surface antigens that are typically expressed on the surface of stem cells were examined. Notably, more than 90% of RPCI-WM1 cells gated were CD110+low and CXCR4/CD184+low. A comparison of these and the remaining stem-cell antigens in BCWM.1 and MWCL-1 cell lines is presented in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0122338#pone.0122338.t004\" target=\"_blank\">Table 4</a>.</p>", "links"=>[], "tags"=>["BCWM .1", "MWCL", "4 intracellular antigens", "WM cell lines", "surface antigen expression", "tumor clone population", "cell surface markers", "cell surface antigens", "flow cytometry analysis", "CD 20 antigens", "tumor cell population", "3 cell lines"], "article_id"=>1371289, "categories"=>["Uncategorised"], "users"=>["Aneel Paulus", "Kasyapa S. Chitta", "Paul K. Wallace", "Pooja P. Advani", "Sharoon Akhtar", "Maja Kuranz-Blake", "Sikander Ailawadhi", "Asher A. Chanan-Khan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122338.g004", "stats"=>{"downloads"=>0, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Presence_of_stem_cell_markers_on_RPCI_WM1_/1371289", "title"=>"Presence of stem-cell markers on RPCI-WM1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-08 02:46:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/2009885"], "description"=>"<p>A total of 65 extracellular and 4 intracellular antigens, comprising B-cell, plasma cell, T-cell, NK-cell, myeloid and hematopoietic stem cell surface markers were analyzed by flow cytometry analysis. Quantification of % gated antigen expression and density of antigen expression is detailed in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0122338#pone.0122338.t004\" target=\"_blank\">Table 4</a>. Blue line indicates RPCI-WM1 antigen expression, red line indicates MWCL-1 antigen expression and green line indicates antigen expression in BCWM.1 cell line.</p>", "links"=>[], "tags"=>["BCWM .1", "MWCL", "4 intracellular antigens", "WM cell lines", "surface antigen expression", "tumor clone population", "cell surface markers", "cell surface antigens", "flow cytometry analysis", "CD 20 antigens", "tumor cell population", "3 cell lines"], "article_id"=>1371288, "categories"=>["Uncategorised"], "users"=>["Aneel Paulus", "Kasyapa S. Chitta", "Paul K. Wallace", "Pooja P. Advani", "Sharoon Akhtar", "Maja Kuranz-Blake", "Sikander Ailawadhi", "Asher A. Chanan-Khan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122338.g003", "stats"=>{"downloads"=>2, "page_views"=>38, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparative_immunophenotyping_analysis_of_RPCI_WM1_BCWM_1_and_MWCL_1_WM_cell_lines_/1371288", "title"=>"Comparative immunophenotyping analysis of RPCI-WM1, BCWM.1 and MWCL-1 WM cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-08 02:46:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/2009880"], "description"=>"<p>Fluorescein (FITC), phycoerythrin (PE), phycoerythrin—cyanine 5 (PC5) or allophycocyanin (APC) conjugates of various antibodies were used as presented above. All cell lines were negative for CD10 and 11c. Blue line indicates RPCI-WM1 antigen expression, red line indicates MWCL-1 antigen expression and green line indicates antigen expression in BCWM.1 cell line. Only BCWM.1 an MWCL-1 were CD19+, CD20+ and FMC7+. Expression of CD138 and κ-light-chain was seen only on MWCL-1 and RPCI-WM1 cells. CD28 expression was markedly more observable on RPCI-WM1 tumor cells as compared to MWCL-1 or BCWM.1.</p>", "links"=>[], "tags"=>["BCWM .1", "MWCL", "4 intracellular antigens", "WM cell lines", "surface antigen expression", "tumor clone population", "cell surface markers", "cell surface antigens", "flow cytometry analysis", "CD 20 antigens", "tumor cell population", "3 cell lines"], "article_id"=>1371283, "categories"=>["Uncategorised"], "users"=>["Aneel Paulus", "Kasyapa S. Chitta", "Paul K. Wallace", "Pooja P. Advani", "Sharoon Akhtar", "Maja Kuranz-Blake", "Sikander Ailawadhi", "Asher A. Chanan-Khan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122338.g002", "stats"=>{"downloads"=>2, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_WM_specific_antigen_expression_compared_across_RPCI_WM1_BCWM_1_and_MWCL_1_cell_lines_/1371283", "title"=>"WM-specific antigen expression compared across RPCI-WM1, BCWM.1 and MWCL-1 cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-08 02:46:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/2009895"], "description"=>"<p>Abbreviations: +, present in >20% gated cells;-, present in <20% of gated cells; LPL/WM, lymphoplasmacytic lymphoma/Waldenström macroglobulinemia; SMZL, splenic marginal zone lymphoma; CLL, chronic lymphocytic leukemia; MCL, mantle cell lymphoma; FL, follicular lymphoma; HCL, hairy cell leukemia; MALT, mucosa-associated lymphoid tissue; MM, multiple myeloma.</p><p>Immunophenotype comparison of common WM models with various B/plasma cell cancers.</p>", "links"=>[], "tags"=>["BCWM .1", "MWCL", "4 intracellular antigens", "WM cell lines", "surface antigen expression", "tumor clone population", "cell surface markers", "cell surface antigens", "flow cytometry analysis", "CD 20 antigens", "tumor cell population", "3 cell lines"], "article_id"=>1371295, "categories"=>["Uncategorised"], "users"=>["Aneel Paulus", "Kasyapa S. Chitta", "Paul K. Wallace", "Pooja P. Advani", "Sharoon Akhtar", "Maja Kuranz-Blake", "Sikander Ailawadhi", "Asher A. Chanan-Khan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0122338.t005", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunophenotype_comparison_of_common_WM_models_with_various_B_plasma_cell_cancers_/1371295", "title"=>"Immunophenotype comparison of common WM models with various B/plasma cell cancers.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-04-08 02:46:31"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"38", "full-text"=>"40", "pdf"=>"26", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"29", "full-text"=>"24", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"26", "full-text"=>"26", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"22", "full-text"=>"17", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"23", "full-text"=>"23", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"28", "full-text"=>"35", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"23", "full-text"=>"20", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"20", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"28", "full-text"=>"30", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"21", "full-text"=>"30", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"26", "full-text"=>"28", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"20", "full-text"=>"25", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"24", "full-text"=>"22", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"33", "full-text"=>"32", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"25", "full-text"=>"20", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"25", "full-text"=>"28", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"21", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"29", "full-text"=>"34", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"25", "full-text"=>"28", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"30", "full-text"=>"32", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"41", "full-text"=>"40", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"40", "full-text"=>"39", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"34", "full-text"=>"32", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"23", "full-text"=>"25", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"22", "full-text"=>"26", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"33", "full-text"=>"32", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"37", "full-text"=>"38", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"4", "cited-by"=>"1", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"36", "full-text"=>"36", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"30", "full-text"=>"30", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"46", "full-text"=>"43", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"43", "full-text"=>"38", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"1", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"44", "full-text"=>"45", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"36", "full-text"=>"38", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"31", "full-text"=>"30", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"29", "full-text"=>"34", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"34", "full-text"=>"39", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"36", "full-text"=>"37", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"37", "full-text"=>"39", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"34", "full-text"=>"32", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"25", "full-text"=>"24", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"23", "full-text"=>"25", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"38", "full-text"=>"40", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"31", "full-text"=>"28", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"1", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts