532 nm Low-Power Laser Irradiation Facilitates the Migration of GABAergic Neural Stem/Progenitor Cells in Mouse Neocortex
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{"title"=>"532 nm Low-Power Laser Irradiation Facilitates the Migration of GABAergic Neural Stem/Progenitor Cells in Mouse Neocortex", "type"=>"journal", "authors"=>[{"first_name"=>"Yumi", "last_name"=>"Fukuzaki"}, {"first_name"=>"Hyeryun", "last_name"=>"Shin"}, {"first_name"=>"Hideki D.", "last_name"=>"Kawai"}, {"first_name"=>"Banri", "last_name"=>"Yamanoha"}, {"first_name"=>"Shinichi", "last_name"=>"Kogure"}], "year"=>2015, "source"=>"PLOS ONE", "identifiers"=>{"doi"=>"10.1371/journal.pone.0123833", "issn"=>"1932-6203"}, "id"=>"05d6e447-8718-3cbe-90fe-67581ecc98fd", "link"=>"http://www.mendeley.com/research/532-nm-lowpower-laser-irradiation-facilitates-migration-gabaergic-neural-stemprogenitor-cells-mouse", "reader_count"=>1, "reader_count_by_academic_status"=>{"Other"=>1}, "reader_count_by_user_role"=>{"Other"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1}, "reader_count_by_subdiscipline"=>{"Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2042531"], "description"=>"<p>A: Western blot analysis of auditory cortex lysates at post-LLI and 2 days after LLI. GAPDH is a loading control. B: Quantification of blots using Image J. Band intensity was normalized to GAPDH (mean ± SD, n = 4, t-test *p < 0.05).</p>", "links"=>[], "tags"=>["adult murine neocortex", "transcranial 532 nm LLI", "migration", "day 14 ganglionic eminence", "proliferation", "532 nm LLI", "cw", "GABAergic NSPCs", "gad", "Akt expression", "Mouse Neocortex Background", "techniques.ResultsIn vivo experiments"], "article_id"=>1396609, "categories"=>["Biological Sciences"], "users"=>["Yumi Fukuzaki", "Hyeryun Shin", "Hideki D. Kawai", "Banri Yamanoha", "Shinichi Kogure"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0123833.g002", "stats"=>{"downloads"=>3, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcranial_LLI_effects_on_pAkt_and_Akt_expression_/1396609", "title"=>"Transcranial LLI effects on pAkt and Akt expression.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-28 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2042533"], "description"=>"<p>A: Location of brain parts dissected for making neurosphere of NSPCs. “a” shows cortical wall of E10 forebrain, which generates excitatory neurons, whereas “b” shows E14 MGE, which generates GABAergic neurons. B: CCK-8 assay to show biochemically cell proliferation for different duration of LLI (mean ± SD, n = 4 for each duration, t-test, *p < 0.05). The non-irradiated group was standardized as 100%. C: Cell counting of DAPI staining to clarify the increase in cell number following the dissociation from neurospheres. LLI significantly promoted proliferation (mean ± SD, n = 5, t-test, *p < 0.05).</p>", "links"=>[], "tags"=>["adult murine neocortex", "transcranial 532 nm LLI", "migration", "day 14 ganglionic eminence", "proliferation", "532 nm LLI", "cw", "GABAergic NSPCs", "gad", "Akt expression", "Mouse Neocortex Background", "techniques.ResultsIn vivo experiments"], "article_id"=>1396611, "categories"=>["Biological Sciences"], "users"=>["Yumi Fukuzaki", "Hyeryun Shin", "Hideki D. Kawai", "Banri Yamanoha", "Shinichi Kogure"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0123833.g003", "stats"=>{"downloads"=>2, "page_views"=>43, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_LLI_effects_on_proliferation_of_NSPC_cells_from_E10_forebrain_and_E14_MGE_/1396611", "title"=>"LLI effects on proliferation of NSPC cells from E10 forebrain and E14 MGE.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-28 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2042534"], "description"=>"<p>A: Location of brain parts dissected for making neurospheres of NSPCs. Label “c” shows the cortex of E16, which includes NSPCs of excitatory and GABAergic neurons. B: Method of trans-well migration test. Bottom membrane of inserted trans-well has pores of 8 μm diameter. Neurospheres derived from “c” were placed on insert trans-well, and 532 nm laser was irradiated from above the media. Cells were fixed by 4% PFA at 48 h after LLI. C: A cell that moved through the pores stained by GAD67 (red) and Hochest (blue). Holes in the Hochest image are the pores of trans-well. Scale bar: 10 μm. Double positive cells were found only in the LLI experimental group (mean ± SD, n = 4, t-test *p < 0.05).</p>", "links"=>[], "tags"=>["adult murine neocortex", "transcranial 532 nm LLI", "migration", "day 14 ganglionic eminence", "proliferation", "532 nm LLI", "cw", "GABAergic NSPCs", "gad", "Akt expression", "Mouse Neocortex Background", "techniques.ResultsIn vivo experiments"], "article_id"=>1396612, "categories"=>["Biological Sciences"], "users"=>["Yumi Fukuzaki", "Hyeryun Shin", "Hideki D. Kawai", "Banri Yamanoha", "Shinichi Kogure"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0123833.g004", "stats"=>{"downloads"=>4, "page_views"=>173, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Trans_well_migration_experiments_/1396612", "title"=>"Trans-well migration experiments.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-28 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2042535"], "description"=>"<p>A: Western blot analysis of neurosphere lysates from post-LLI (4 hours) of E10 forebrain. B: Quantification of blots. Each blot intensity was normalized to GAPDH of loading control (mean ± SD, n = 3, t-test *p < 0.05).</p>", "links"=>[], "tags"=>["adult murine neocortex", "transcranial 532 nm LLI", "migration", "day 14 ganglionic eminence", "proliferation", "532 nm LLI", "cw", "GABAergic NSPCs", "gad", "Akt expression", "Mouse Neocortex Background", "techniques.ResultsIn vivo experiments"], "article_id"=>1396613, "categories"=>["Biological Sciences"], "users"=>["Yumi Fukuzaki", "Hyeryun Shin", "Hideki D. Kawai", "Banri Yamanoha", "Shinichi Kogure"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0123833.g005", "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_LLI_effects_on_pAkt_and_Akt_expression_of_cultured_cells_/1396613", "title"=>"LLI effects on pAkt and Akt expression of cultured cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-28 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2042538", "https://ndownloader.figshare.com/files/2042539", "https://ndownloader.figshare.com/files/2042540", "https://ndownloader.figshare.com/files/2042541", "https://ndownloader.figshare.com/files/2042542", "https://ndownloader.figshare.com/files/2042543", "https://ndownloader.figshare.com/files/2042544", "https://ndownloader.figshare.com/files/2042545"], "description"=>"<div><p>Background and Objective</p><p>Accumulating evidence has shown that low-power laser irradiation (LLI) affects cell proliferation and survival, but little is known about LLI effects on neural stem/progenitor cells (NSPCs). Here we investigate whether transcranial 532 nm LLI affects NSPCs in adult murine neocortex and in neurospheres from embryonic mice.</p><p>Study Design/Materials and Methods</p><p>We applied 532 nm LLI (Nd:YVO<sub>4</sub>, CW, 60 mW) on neocortical surface via cranium in adult mice and on cultured cells from embryonic mouse brains in vitro to investigate the proliferation and migration of NSPCs and Akt expression using immunohistochemical assays and Western blotting techniques.</p><p>Results</p><p>In vivo experiments demonstrated that 532 nm LLI significantly facilitated the migration of GABAergic NSPCs that were induced to proliferate in layer 1 by mild ischemia. In vitro experiments using GABAergic NSPCs derived from embryonic day 14 ganglionic eminence demonstrated that 532 nm LLI for 60 min promoted the migration of GAD67-immunopositive NSPCs with a significant increase of Akt expression. Meanwhile, the LLI induced proliferation, but not migration, of NSPCs that give rise to excitatory neurons.</p><p>Conclusion</p><p>It is concluded that 532 nm LLI promoted the migration of GABAergic NSPCs into deeper layers of the neocortex in vivo by elevating Akt expression.</p></div>", "links"=>[], "tags"=>["adult murine neocortex", "transcranial 532 nm LLI", "migration", "day 14 ganglionic eminence", "proliferation", "532 nm LLI", "cw", "GABAergic NSPCs", "gad", "Akt expression", "Mouse Neocortex Background", "techniques.ResultsIn vivo experiments"], "article_id"=>1396616, "categories"=>["Biological Sciences"], "users"=>["Yumi Fukuzaki", "Hyeryun Shin", "Hideki D. Kawai", "Banri Yamanoha", "Shinichi Kogure"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0123833.s001", "https://dx.doi.org/10.1371/journal.pone.0123833.s002", "https://dx.doi.org/10.1371/journal.pone.0123833.s003", "https://dx.doi.org/10.1371/journal.pone.0123833.s004", "https://dx.doi.org/10.1371/journal.pone.0123833.s005", "https://dx.doi.org/10.1371/journal.pone.0123833.s006", "https://dx.doi.org/10.1371/journal.pone.0123833.s007", "https://dx.doi.org/10.1371/journal.pone.0123833.s008"], "stats"=>{"downloads"=>13, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_532_nm_Low_Power_Laser_Irradiation_Facilitates_the_Migration_of_GABAergic_Neural_Stem_Progenitor_Cells_in_Mouse_Neocortex_/1396616", "title"=>"532 nm Low-Power Laser Irradiation Facilitates the Migration of GABAergic Neural Stem/Progenitor Cells in Mouse Neocortex", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-04-28 02:42:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/2042529"], "description"=>"<p>A: Scheme of LLI treatment to adult mouse brain. Auditory cortical area was irradiated on the left side of the cortex through cranium. The opposite hemisphere was used as a control. B: TTC staining for detecting cell death. LLI after the mild occlusion of CCA (CCAO) did not induce cell death (left) compared with sham control (right). C: Protocol for in vivo experiments from CCAO to immunostaining. Brains were fixed at 4 h and 5 days after LLI. D: Photographs of each immunostaining at post-LLI (4 hours) and 5 days after LLI. EdU, GAD67 and Ki67 positive cells (white arrow) were found in layer 1 of post-LLI sections. EdU and GAD67 positive cells but Ki67 negative cells (yellow arrow) were found in deep layer at 5 days after LLI. GAD67 positive, EdU and Ki67 negative cell (arrowhead) is a mature inhibitory neuron. Scale bars: 10 μm. E: The laminar pattern of the cell density (mean ± SEM, n = 4) of EdU and GAD67 positive, Ki67-negative cells from layer 1 to 6 was significantly different between LLI and control groups (KS test, p<0.01). F: Cumulative fraction of total cells for non-parametric KS 2-sample test. Each accumulative curve shows higher distribution in layer 1–4 in control group, while does in layer 5–6 in LLI group. G: Total cell density of EdU and GAD67 positive and Ki67 negative (EdU+/GAD67+/Ki67-) cells did not differ between control and LLI groups.</p>", "links"=>[], "tags"=>["adult murine neocortex", "transcranial 532 nm LLI", "migration", "day 14 ganglionic eminence", "proliferation", "532 nm LLI", "cw", "GABAergic NSPCs", "gad", "Akt expression", "Mouse Neocortex Background", "techniques.ResultsIn vivo experiments"], "article_id"=>1396607, "categories"=>["Biological Sciences"], "users"=>["Yumi Fukuzaki", "Hyeryun Shin", "Hideki D. Kawai", "Banri Yamanoha", "Shinichi Kogure"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0123833.g001", "stats"=>{"downloads"=>2, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_transcranial_LLI_on_cell_migration_of_NSPCs_/1396607", "title"=>"Effects of transcranial LLI on cell migration of NSPCs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-04-28 02:42:41"}

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Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[]}
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