Positive Selection of Natural Poly-Reactive B Cells in the Periphery Occurs Independent of Heavy Chain Allelic Inclusion
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Positive selection of natural poly-reactive B Cells in the periphery occurs independent of heavy chain allelic inclusion", "type"=>"journal", "authors"=>[{"first_name"=>"Ying", "last_name"=>"Xing", "scopus_author_id"=>"35314140800"}, {"first_name"=>"Qiuhe", "last_name"=>"Ji", "scopus_author_id"=>"15822229300"}, {"first_name"=>"Ying", "last_name"=>"Lin", "scopus_author_id"=>"55714568100"}, {"first_name"=>"Meng", "last_name"=>"Fu", "scopus_author_id"=>"35761849800"}, {"first_name"=>"Jixin", "last_name"=>"Gao", "scopus_author_id"=>"55616974700"}, {"first_name"=>"Ping", "last_name"=>"Zhang", "scopus_author_id"=>"55721514600"}, {"first_name"=>"Xingbin", "last_name"=>"Hu", "scopus_author_id"=>"13409928000"}, {"first_name"=>"Lei", "last_name"=>"Feng", "scopus_author_id"=>"57199898305"}, {"first_name"=>"Yufeng", "last_name"=>"Liu", "scopus_author_id"=>"23470300600"}, {"first_name"=>"Hua", "last_name"=>"Han", "scopus_author_id"=>"7401969486"}, {"first_name"=>"Wei", "last_name"=>"Li", "scopus_author_id"=>"56499621300"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84930685794", "doi"=>"10.1371/journal.pone.0125747", "pui"=>"604612939", "sgr"=>"84930685794"}, "id"=>"d1808c7c-9af9-35ab-be90-2741c0bce058", "abstract"=>"© 2015 Xing et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Natural autoreactive B cells are important mediators of autoimmune diseases. Receptor editing is known to play an important role in both central and peripheral B cell tolerance. However, the role of allelic inclusion in the development of natural autoreactive B cells is not clear. Previously, we generated μ chain (TgV < inf > H < /inf > 3B4I) and μ/κ chains (TgV < inf > H/L < /inf > 3B4) transgenic mice using transgene derived from the 3B4 hybridoma, which produce poly-reactive natural autoantibodies. In this study, we demonstrate that a considerable population of B cells edited their B cells receptors (BCRs) via light chain or heavy chain allelic inclusion during their development in TgV < inf > H < /inf > 3B4I mice. Additionally, allelic inclusion occurred more frequently in the periphery and promoted the differentiation of B cells into marginal zone or B-1a cells in TgV < inf > H < /inf > 3B4I mice. B cells from TgV < inf > H/L < /inf > 3B4 mice expressing the intact transgenic 3B4 BCR without receptor editing secreted poly-reactive 3B4 antibody. Interestingly, however, B cell that underwent allelic inclusion in TgV < inf > H < /inf > 3B4I mice also produced poly-reactive autoantibodies in vivo and in vitro. Our findings suggest that receptor editing plays a minor role in the positive selection of B cells expressing natural poly-reactive BCRs, which can be positively selected through heavy chain allelic inclusion to retain their poly-reactivity in the periphery.", "link"=>"http://www.mendeley.com/research/positive-selection-natural-polyreactive-b-cells-periphery-occurs-independent-heavy-chain-allelic-inc", "reader_count"=>3, "reader_count_by_academic_status"=>{"Researcher"=>2, "Student > Master"=>1}, "reader_count_by_user_role"=>{"Researcher"=>2, "Student > Master"=>1}, "reader_count_by_subject_area"=>{"Agricultural and Biological Sciences"=>2, "Medicine and Dentistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84930685794"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84930685794?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84930685794&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84930685794&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84930685794", "dc:identifier"=>"SCOPUS_ID:84930685794", "eid"=>"2-s2.0-84930685794", "dc:title"=>"Positive selection of natural poly-reactive B Cells in the periphery occurs independent of heavy chain allelic inclusion", "dc:creator"=>"Xing Y.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"5", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-05-19", "prism:coverDisplayDate"=>"19 May 2015", "prism:doi"=>"10.1371/journal.pone.0125747", "citedby-count"=>"0", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"The Fourth Military Medical University", "affiliation-city"=>"Xi'an", "affiliation-country"=>"China"}, {"@_fa"=>"true", "affilname"=>"Xijing Hospital", "affiliation-city"=>"Xi'an", "affiliation-country"=>"China"}], "pubmed-id"=>"25993514", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0125747", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0125747", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"29", "xml_views"=>"8", "html_views"=>"227"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"80"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"3", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"0"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2074707"], "description"=>"<p>(A) Allelic inclusion of IgH chain on peripheral B cells. Cells from spleen, PEC and peripheral blood of the indicated mice were labeled with anti-IgM<sup>a</sup>, anti-IgM<sup>b</sup>, and anti-CD19, and were analyzed by FACS. CD19<sup>+</sup> cells were gated on lymphocytes. The numbers indicate the percentage of cells in total CD19<sup>+</sup> cells within the indicated gates. (B) L chain expression in B cells from TgV<sub>H</sub>3B4I. Cells were stained with anti-κ, anti-λ and anti-CD19 antibodies and were analyzed, as in A. (C) λ-expressing cells are mainly IgM<sup>a+</sup> in TgV<sub>H</sub>3B4I mice. Histograms show λ chain expression in IgM<sup>b+</sup> (solid fill) and IgM<sup>a+</sup> (no fill, including IgM<sup>a+</sup>IgM<sup>b+</sup> and IgM<sup>a+</sup>IgM<sup>b-</sup>) cells from splenocytes and PEC of TgV<sub>H</sub>3B4I mice. Data are representative of more than eight independent experiments with at least three mice in each group.</p>", "links"=>[], "tags"=>["allelic inclusion", "3 B 4 hybridoma", "b cells", "autoreactive B cells", "3 B 4 BCR", "Periphery Occurs Independent", "receptor editing", "chain allelic inclusion", "B cell tolerance", "Heavy Chain Allelic Inclusion Natural autoreactive B cells", "B cells receptors", "TgVH 3B mice"], "article_id"=>1420081, "categories"=>["Biological Sciences"], "users"=>["Ying Xing", "Qiuhe Ji", "Ying Lin", "Meng Fu", "Jixin Gao", "Ping Zhang", "Xingbin Hu", "Lei Feng", "Yufeng Liu", "Hua Han", "Wei Li"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0125747.g001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_H_and_L_chain_expression_on_peripheral_B_cells_from_TgV_H_3B4I_/1420081", "title"=>"H and L chain expression on peripheral B cells from TgV<sub>H</sub>3B4I.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-05-19 03:15:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/2074708"], "description"=>"<p>(A) Phenotypic analysis of IgM<sup>a+</sup>IgM<sup>b-</sup> (solid fill) and IgM<sup>a+</sup>IgM<sup>b+</sup> (no fill) B cells for the indicated cell surface markers by flow cytometry from splenocytes of TgV<sub>H</sub>3B4I mice. (B) IgM<sup>a+</sup>IgM<sup>b+</sup> B cells localized in the MZ of spleen of TgV<sub>H</sub>3B4I. Analysis of frozen spleen sections from TgV<sub>H</sub>3B4I mice, stained with anti-IgM<sup>a</sup> (red) and anti-IgM<sup>b</sup> (green), using confocal microscopy. (C) CD5 was expressed predominantly on IgM<sup>a-</sup>IgM<sup>b+</sup> and allelic included IgM<sup>a+</sup>IgM<sup>b+</sup> B cells. Representative histograms show the surface expression of CD5 on IgM<sup>a-</sup>IgM<sup>b+</sup> (solid fill), IgM<sup>a+</sup>IgM<sup>b-</sup> (solid line) and IgM<sup>a+</sup>IgM<sup>b+</sup> (dotted line) B cells of PEC from TgV<sub>H</sub>3B4I mice. Data are representative of more than five independent experiments with three mice per group.</p>", "links"=>[], "tags"=>["allelic inclusion", "3 B 4 hybridoma", "b cells", "autoreactive B cells", "3 B 4 BCR", "Periphery Occurs Independent", "receptor editing", "chain allelic inclusion", "B cell tolerance", "Heavy Chain Allelic Inclusion Natural autoreactive B cells", "B cells receptors", "TgVH 3B mice"], "article_id"=>1420082, "categories"=>["Biological Sciences"], "users"=>["Ying Xing", "Qiuhe Ji", "Ying Lin", "Meng Fu", "Jixin Gao", "Ping Zhang", "Xingbin Hu", "Lei Feng", "Yufeng Liu", "Hua Han", "Wei Li"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0125747.g002", "stats"=>{"downloads"=>1, "page_views"=>31, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Accumulation_of_B_cells_with_allelic_inclusion_in_the_MZ_of_spleen_and_their_differentiation_into_B_1a_subset_in_PEC_of_TgV_H_3B4I_mice_/1420082", "title"=>"Accumulation of B cells with allelic inclusion in the MZ of spleen and their differentiation into B-1a subset in PEC of TgV<sub>H</sub>3B4I mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-05-19 03:15:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/2074709"], "description"=>"<p>BM cells from the indicated mouse strains were stained with anti-B220, anti-CD43, and anti-IgM, and were analyzed by FACS. (A) The gates in panels represent Hardy fractions A-D (proB to preBII), and can be divided into fraction A-C (proB to preBI, upper rectangle) and fraction D (preBII, lower rectangle) by the expression of CD43. The numbers indicate the percentage of cells within the gates. (B) Hardy fractions D (preBII, lower left panels), F (circulating mature B cells, upper middle panels) and E (immature B cells, lower right panels) of the indicated mice. (C) Decreased absolute numbers of total BM B cells in transgenic mice. Numbers of B220<sup>+</sup> cells were calculated and shown. (D) Numbers of preBII and immature B cells in B were calculated, and the ratios of immature cells to preBII cells were calculated as shown. Error bars represent means ± SD, *<i>P</i><0.05, **<i>P</i><0.01, n = 5. (E) FACS analysis of BM B cells from the indicated transgenic mice. CD23<sup>-</sup> (newly formed) and CD23<sup>+</sup> (mature) B cells in the B220<sup>+</sup> gate are shown in the boxes. Data are representative of more than five independent experiments.</p>", "links"=>[], "tags"=>["allelic inclusion", "3 B 4 hybridoma", "b cells", "autoreactive B cells", "3 B 4 BCR", "Periphery Occurs Independent", "receptor editing", "chain allelic inclusion", "B cell tolerance", "Heavy Chain Allelic Inclusion Natural autoreactive B cells", "B cells receptors", "TgVH 3B mice"], "article_id"=>1420083, "categories"=>["Biological Sciences"], "users"=>["Ying Xing", "Qiuhe Ji", "Ying Lin", "Meng Fu", "Jixin Gao", "Ping Zhang", "Xingbin Hu", "Lei Feng", "Yufeng Liu", "Hua Han", "Wei Li"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0125747.g003", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Assessment_of_B_cell_development_in_the_BM_of_transgenic_mice_/1420083", "title"=>"Assessment of B cell development in the BM of transgenic mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-05-19 03:15:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/2074714"], "description"=>"<p>(A) Expression of H chains in the peripheral B cells. Cells from spleen, PEC and peripheral blood of TgV<sub>H/L</sub>3B4 mice were stained and analyzed as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0125747#pone.0125747.g001\" target=\"_blank\">Fig 1A</a>. (B-C) Bar graph shows the absolute numbers of total, IgM<sup>a+</sup>IgM<sup>b-</sup> and IgM<sup>a+</sup>IgM<sup>b+</sup> B cells as gated in A in the spleen (B) and PEC (C) of the indicated mice. Error bars show mean ±SD, *<i>P</i><0.05. (D) L chain expression in B cells from TgV<sub>H/L</sub>3B4 mice. Cells were stained with anti-κ, anti-λ and anti-CD19, and were analyzed by FACS. (E) RT-PCR analysis of Rag2 mRNA expression in B cells of spleen and BM from transgenic mice and littermates. Data are representatives of five independent experiments.</p>", "links"=>[], "tags"=>["allelic inclusion", "3 B 4 hybridoma", "b cells", "autoreactive B cells", "3 B 4 BCR", "Periphery Occurs Independent", "receptor editing", "chain allelic inclusion", "B cell tolerance", "Heavy Chain Allelic Inclusion Natural autoreactive B cells", "B cells receptors", "TgVH 3B mice"], "article_id"=>1420085, "categories"=>["Biological Sciences"], "users"=>["Ying Xing", "Qiuhe Ji", "Ying Lin", "Meng Fu", "Jixin Gao", "Ping Zhang", "Xingbin Hu", "Lei Feng", "Yufeng Liu", "Hua Han", "Wei Li"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0125747.g004", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_High_allelic_exclusion_of_H_and_L_chains_in_peripheral_B_cells_of_TgV_H_L_3B4_mice_/1420085", "title"=>"High allelic exclusion of H and L chains in peripheral B cells of TgV<sub>H/L</sub>3B4 mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-05-19 03:15:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/2074728"], "description"=>"<p>(A) The phenotype of B cells in the spleen of TgV<sub>H</sub>3B4I and TgV<sub>H/L</sub>3B4 mice. CD19<sup>+</sup> or IgM<sup>a+</sup> splenic B cells from indicated mice were analyzed for the expression of CD21 and CD23 by FACS. Numbers next to each gate indicate the percentage of cells in that gate in total CD19<sup>+</sup> or IgM<sup>a+</sup> cells. At least 7 mice from each genotype were analyzed. (B) Spleen sections from the indicated mice were stained with anti-MOMA1-FITC and anti-B220-Biotin (left panels) or anti-IgM<sup>a</sup>-biotin (right panel) followed by streptavidin-Cy3, and acquired using fluorescence microscopy. Follicular (Fo) areas around PALS are shown and MZ is indicated with arrow. The original magnitude was ×200 or ×400 as indicated. (C) CD19<sup>+</sup> or IgM<sup>a+</sup> B cells in PEC of indicated mice were evaluated for the expression of CD5 by FACS. At least 5 mice from each genotype were analyzed. Numbers next to each gate indicate the percentage of cells in that gate in total CD19<sup>+</sup> or IgM<sup>a+</sup> cells.</p>", "links"=>[], "tags"=>["allelic inclusion", "3 B 4 hybridoma", "b cells", "autoreactive B cells", "3 B 4 BCR", "Periphery Occurs Independent", "receptor editing", "chain allelic inclusion", "B cell tolerance", "Heavy Chain Allelic Inclusion Natural autoreactive B cells", "B cells receptors", "TgVH 3B mice"], "article_id"=>1420087, "categories"=>["Biological Sciences"], "users"=>["Ying Xing", "Qiuhe Ji", "Ying Lin", "Meng Fu", "Jixin Gao", "Ping Zhang", "Xingbin Hu", "Lei Feng", "Yufeng Liu", "Hua Han", "Wei Li"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0125747.g005", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_B_cells_development_in_spleen_and_PEC_of_transgenic_mice_/1420087", "title"=>"Analysis of B cells development in spleen and PEC of transgenic mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-05-19 03:15:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/2074731"], "description"=>"<p>(A) ELISA. Sera from at least 5 mice from each genotype were collected, and analyzed with ELISA using standard protocols. (B) In vitro secretion of autoantibodies by B cells. Purified B cells from spleen and PEC of indicated mice were treated with LPS, anti-IgM and anti-CD40+IL-4 for 3 days. Supernatants were collected and the concentration of secreted antibodies was assessed by ELISA. (C) B cells were sorted from spleens of TgV<sub>H</sub>3B4I mice and were treated with LPS, and antibody production was tested as in B. Error bars show means ± SD. *<i>P</i><0.05, **<i>P</i><0.01, n = 5. (D) Calcium mobilization analysis. Sorted splenic B cells from TgV<sub>H</sub>3B4 mice and littermate controls were stained with Fluo3. Cells were stimulation with actin (20 μg/ml) and analyzed by FACS.</p>", "links"=>[], "tags"=>["allelic inclusion", "3 B 4 hybridoma", "b cells", "autoreactive B cells", "3 B 4 BCR", "Periphery Occurs Independent", "receptor editing", "chain allelic inclusion", "B cell tolerance", "Heavy Chain Allelic Inclusion Natural autoreactive B cells", "B cells receptors", "TgVH 3B mice"], "article_id"=>1420090, "categories"=>["Biological Sciences"], "users"=>["Ying Xing", "Qiuhe Ji", "Ying Lin", "Meng Fu", "Jixin Gao", "Ping Zhang", "Xingbin Hu", "Lei Feng", "Yufeng Liu", "Hua Han", "Wei Li"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0125747.g006", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Antibody_production_by_allelic_included_B_cells_in_TgV_H_3B4I_/1420090", "title"=>"Antibody production by allelic included B cells in TgV<sub>H</sub>3B4I.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-05-19 03:15:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/2074732", "https://ndownloader.figshare.com/files/2074733", "https://ndownloader.figshare.com/files/2074734", "https://ndownloader.figshare.com/files/2074735"], "description"=>"<div><p>Natural autoreactive B cells are important mediators of autoimmune diseases. Receptor editing is known to play an important role in both central and peripheral B cell tolerance. However, the role of allelic inclusion in the development of natural autoreactive B cells is not clear. Previously, we generated μ chain (TgV<sub>H</sub>3B4I) and μ/κ chains (TgV<sub>H/L</sub>3B4) transgenic mice using transgene derived from the 3B4 hybridoma, which produce poly-reactive natural autoantibodies. In this study, we demonstrate that a considerable population of B cells edited their B cells receptors (BCRs) via light chain or heavy chain allelic inclusion during their development in TgV<sub>H</sub>3B4I mice. Additionally, allelic inclusion occurred more frequently in the periphery and promoted the differentiation of B cells into marginal zone or B-1a cells in TgV<sub>H</sub>3B4I mice. B cells from TgV<sub>H/L</sub>3B4 mice expressing the intact transgenic 3B4 BCR without receptor editing secreted poly-reactive 3B4 antibody. Interestingly, however, B cell that underwent allelic inclusion in TgV<sub>H</sub>3B4I mice also produced poly-reactive autoantibodies in vivo and in vitro. Our findings suggest that receptor editing plays a minor role in the positive selection of B cells expressing natural poly-reactive BCRs, which can be positively selected through heavy chain allelic inclusion to retain their poly-reactivity in the periphery.</p></div>", "links"=>[], "tags"=>["allelic inclusion", "3 B 4 hybridoma", "b cells", "autoreactive B cells", "3 B 4 BCR", "Periphery Occurs Independent", "receptor editing", "chain allelic inclusion", "B cell tolerance", "Heavy Chain Allelic Inclusion Natural autoreactive B cells", "B cells receptors", "TgVH 3B mice"], "article_id"=>1420091, "categories"=>["Biological Sciences"], "users"=>["Ying Xing", "Qiuhe Ji", "Ying Lin", "Meng Fu", "Jixin Gao", "Ping Zhang", "Xingbin Hu", "Lei Feng", "Yufeng Liu", "Hua Han", "Wei Li"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0125747.s001", "https://dx.doi.org/10.1371/journal.pone.0125747.s002", "https://dx.doi.org/10.1371/journal.pone.0125747.s003", "https://dx.doi.org/10.1371/journal.pone.0125747.s004"], "stats"=>{"downloads"=>1, "page_views"=>27, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Positive_Selection_of_Natural_Poly_Reactive_B_Cells_in_the_Periphery_Occurs_Independent_of_Heavy_Chain_Allelic_Inclusion_/1420091", "title"=>"Positive Selection of Natural Poly-Reactive B Cells in the Periphery Occurs Independent of Heavy Chain Allelic Inclusion", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-05-19 03:15:12"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"2", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"22", "full-text"=>"19", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"0", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner

Net::HTTPInternalServerError

Source
Counter
Time
2019-04-06 02:41:42 UTC
Target URL
http://counter-101.soma.plos.org/api/v1.0/stats/doi/10.1371%2Fjournal.pone.0125747
Trace

/app/models/concerns/networkable.rb:21:in `get_result'
/app/models/source.rb:165:in `get_data'
/app/models/retrieval_status.rb:47:in `perform_get_data'
/app/jobs/source_job.rb:52:in `block (2 levels) in perform'
/app/jobs/source_job.rb:51:in `block in perform'
/app/jobs/source_job.rb:35:in `each'
/app/jobs/source_job.rb:35:in `perform'