Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture
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{"title"=>"Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture", "type"=>"journal", "authors"=>[{"first_name"=>"Aurélie", "last_name"=>"Crabbé", "scopus_author_id"=>"25926314000"}, {"first_name"=>"Yulong", "last_name"=>"Liu", "scopus_author_id"=>"57196311468"}, {"first_name"=>"Shameema F.", "last_name"=>"Sarker", "scopus_author_id"=>"7102953265"}, {"first_name"=>"Nicholas R.", "last_name"=>"Bonenfant", "scopus_author_id"=>"55573558800"}, {"first_name"=>"Jennifer", "last_name"=>"Barrila", "scopus_author_id"=>"6504091761"}, {"first_name"=>"Zachary D.", "last_name"=>"Borg", "scopus_author_id"=>"36454654400"}, {"first_name"=>"James J.", "last_name"=>"Lee", "scopus_author_id"=>"55955819300"}, {"first_name"=>"Daniel J.", "last_name"=>"Weiss", "scopus_author_id"=>"57192810603"}, {"first_name"=>"Cheryl A.", "last_name"=>"Nickerson", "scopus_author_id"=>"7006549730"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84930683942", "pui"=>"604612072", "doi"=>"10.1371/journal.pone.0126846", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "sgr"=>"84930683942", "pmid"=>"25962111"}, "id"=>"ee94aec4-712f-3d52-a694-2f3fc9cfeffd", "abstract"=>"Strategies are needed to improve repopulation of decellularized lung scaffolds with stromal and functional epithelial cells. We demonstrate that decellularized mouse lungs recellularized in a dynamic low fluid shear suspension bioreactor, termed the rotating wall vessel (RWV), contained more cells with decreased apoptosis, increased proliferation and enhanced levels of total RNA compared to static recellularization conditions. These results were observed with two relevant mouse cell types: bone marrow-derived mesenchymal stromal (stem) cells (MSCs) and alveolar type II cells (C10). In addition, MSCs cultured in decellularized lungs under static but not bioreactor conditions formed multilayered aggregates. Gene expression and immunohistochemical analyses suggested differentiation of MSCs into collagen I-producing fibroblast-like cells in the bioreactor, indicating enhanced potential for remodeling of the decellularized scaffold matrix. In conclusion, dynamic suspension culture is promising for enhancing repopulation of decellularized lungs, and could contribute to remodeling the extracellular matrix of the scaffolds with subsequent effects on differentiation and functionality of inoculated cells.", "link"=>"http://www.mendeley.com/research/recellularization-decellularized-lung-scaffolds-enhanced-dynamic-suspension-culture", "reader_count"=>45, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>10, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>1, "Student > Master"=>10, "Student > Bachelor"=>7}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>10, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>1, "Student > Master"=>10, "Student > Bachelor"=>7}, "reader_count_by_subject_area"=>{"Engineering"=>12, "Unspecified"=>6, "Biochemistry, Genetics and Molecular Biology"=>6, "Materials Science"=>1, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>2, "Chemistry"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>12}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>6}}, "reader_count_by_country"=>{"United Kingdom"=>1}, "group_count"=>2}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2061616"], "description"=>"<p>* P < 0.08</p><p>** P < 0.05</p><p>*** P < 0.01</p><p>Bold = upregulated compared to MSC monolayer; Italic = downregulated compared to MSC monolayer; White empty cell: P > 0.08 and/or fold-change < 1.5; While cell with ∞: Present in ML, absent in ≥ 50% of samples; White cell with Ф: Not expressed in ML and sample, but expressed in lung.</p><p>Relative expression of target genes in MSCs grown on decellularized lung scaffolds in static and bioreactor conditions as compared to monolayers.</p>", "links"=>[], "tags"=>["decellularized lungs", "decellularized mouse lungs recellularized", "Decellularized Lung Scaffolds", "fluid shear suspension bioreactor", "rwv", "alveolar type II cells", "msc", "Dynamic Suspension Culture Strategies", "mouse cell types", "rna", "decellularized scaffold matrix"], "article_id"=>1410048, "categories"=>["Biological Sciences"], "users"=>["Aurélie Crabbé", "Yulong Liu", "Shameema F. Sarker", "Nicholas R. Bonenfant", "Jennifer Barrila", "Zachary D. Borg", "James J. Lee", "Daniel J. Weiss", "Cheryl A. Nickerson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0126846.t001", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_expression_of_target_genes_in_MSCs_grown_on_decellularized_lung_scaffolds_in_static_and_bioreactor_conditions_as_compared_to_monolayers_/1410048", "title"=>"Relative expression of target genes in MSCs grown on decellularized lung scaffolds in static and bioreactor conditions as compared to monolayers.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-05-11 03:10:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2061611"], "description"=>"<p>An inset in Fig 4Ab with higher magnification is shown to demonstrate that a majority of the cells stained positive for PCNA. Cell nuclei are labeled in blue; marker of interest is labeled in green. Magnifications are 400x. Overlap of cell nucleus and marker of interest can generate green or white color. For each condition, images are representative of the entire lung.</p>", "links"=>[], "tags"=>["decellularized lungs", "decellularized mouse lungs recellularized", "Decellularized Lung Scaffolds", "fluid shear suspension bioreactor", "rwv", "alveolar type II cells", "msc", "Dynamic Suspension Culture Strategies", "mouse cell types", "rna", "decellularized scaffold matrix"], "article_id"=>1410043, "categories"=>["Biological Sciences"], "users"=>["Aurélie Crabbé", "Yulong Liu", "Shameema F. Sarker", "Nicholas R. Bonenfant", "Jennifer Barrila", "Zachary D. Borg", "James J. Lee", "Daniel J. Weiss", "Cheryl A. Nickerson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0126846.g004", "stats"=>{"downloads"=>2, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Annexin_V_and_PCNA_staining_of_decellularized_lung_scaffolds_recellularized_with_A_MSCs_in_static_versus_bioreactor_conditions_for_14_panels_A_B_for_annexin_V_and_a_b_for_PCNA_and_28_days_panels_C_D_for_annexin_V_and_c_d_for_PCNA_single_cells_B_MSC_cell_/1410043", "title"=>"Annexin V and PCNA staining of decellularized lung scaffolds recellularized with (A) MSCs in static versus bioreactor conditions for 14 (panels A, B for annexin V, and a, b for PCNA) and 28 days (panels C, D for annexin V, and c, d for PCNA) (single cells), (B) MSC cell clusters in static conditions at 14 days (panel A for annexin V, and a for PCNA), (C) C10 cells in static (panel A for annexin V, a for PCNA) versus bioreactor (panel B for annexin V, b for PCNA) conditions for 11 days.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-05-11 03:10:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2061599"], "description"=>"<p>MSCs or C10 cells were introduced in the decellularized lung scaffolds through the cannulated trachea. Next, lungs were statically incubated for 4 days, regardless of the subsequent test condition. Culture medium for MSCs was IMDM and for C10 cells GTSF-2. Different time points were tested to assess recellularization with MSCs (3, 10, 24 days) or C10 cells (7, 10 days) in static or bioreactor conditions.</p>", "links"=>[], "tags"=>["decellularized lungs", "decellularized mouse lungs recellularized", "Decellularized Lung Scaffolds", "fluid shear suspension bioreactor", "rwv", "alveolar type II cells", "msc", "Dynamic Suspension Culture Strategies", "mouse cell types", "rna", "decellularized scaffold matrix"], "article_id"=>1410033, "categories"=>["Biological Sciences"], "users"=>["Aurélie Crabbé", "Yulong Liu", "Shameema F. Sarker", "Nicholas R. Bonenfant", "Jennifer Barrila", "Zachary D. Borg", "James J. Lee", "Daniel J. Weiss", "Cheryl A. Nickerson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0126846.g001", "stats"=>{"downloads"=>1, "page_views"=>92, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Overview_of_experimental_set_up_used_to_recellularize_decellularized_lung_scaffolds_with_MSCs_or_C10_cells_in_static_and_bioreactor_conditions_/1410033", "title"=>"Overview of experimental set-up used to recellularize decellularized lung scaffolds with MSCs or C10 cells in static and bioreactor conditions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-05-11 03:10:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2061609"], "description"=>"<p>For each condition, mean RNA levels +/- standard deviation for the left lung is presented. * p < 0.05, ** p < 0.01.</p>", "links"=>[], "tags"=>["decellularized lungs", "decellularized mouse lungs recellularized", "Decellularized Lung Scaffolds", "fluid shear suspension bioreactor", "rwv", "alveolar type II cells", "msc", "Dynamic Suspension Culture Strategies", "mouse cell types", "rna", "decellularized scaffold matrix"], "article_id"=>1410041, "categories"=>["Biological Sciences"], "users"=>["Aurélie Crabbé", "Yulong Liu", "Shameema F. Sarker", "Nicholas R. Bonenfant", "Jennifer Barrila", "Zachary D. Borg", "James J. Lee", "Daniel J. Weiss", "Cheryl A. Nickerson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0126846.g003", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Total_RNA_levels_of_decellularized_lungs_recellularized_with_MSCs_A_or_C10_cells_B_in_static_versus_bioreactor_conditions_/1410041", "title"=>"Total RNA levels of decellularized lungs recellularized with MSCs (A) or C10 cells (B) in static versus bioreactor conditions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-05-11 03:10:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2061608"], "description"=>"<p>For each condition, a low (100x) and high magnification (630x) are shown (e.g., A is low magnification, B is high magnification). Insets are included to show phenotypes at the single cell level. Black arrows point to MSC cell aggregation observed in static recellularization conditions. Blue arrows point to cytoplasmic vacuoles indicative of cell stress. Airways are labelled. For each condition, images are representative of the entire lung, with the exception of panels AA and AB, which reflect a region with high cell density whereas some regions were devoid of cells (not shown).</p>", "links"=>[], "tags"=>["decellularized lungs", "decellularized mouse lungs recellularized", "Decellularized Lung Scaffolds", "fluid shear suspension bioreactor", "rwv", "alveolar type II cells", "msc", "Dynamic Suspension Culture Strategies", "mouse cell types", "rna", "decellularized scaffold matrix"], "article_id"=>1410040, "categories"=>["Biological Sciences"], "users"=>["Aurélie Crabbé", "Yulong Liu", "Shameema F. Sarker", "Nicholas R. Bonenfant", "Jennifer Barrila", "Zachary D. Borg", "James J. Lee", "Daniel J. Weiss", "Cheryl A. Nickerson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0126846.g002", "stats"=>{"downloads"=>0, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Hematoxylin_eosin_staining_of_decellularized_lungs_recellularized_with_A_MSCs_in_static_panels_A_to_F_and_bioreactor_panels_a_to_f_conditions_for_7_14_and_28_days_and_B_with_C10_cells_in_static_panels_A_to_D_or_bioreactor_panels_a_to_d_conditions_for_11_/1410040", "title"=>"Hematoxylin-eosin staining of decellularized lungs recellularized with (A) MSCs in static (panels A to F) and bioreactor (panels a to f) conditions for 7, 14, and 28 days, and (B) with C10 cells in static (panels A to D) or bioreactor (panels a to d) conditions for 11 and 14 days.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-05-11 03:10:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2061617", "https://ndownloader.figshare.com/files/2061618"], "description"=>"<div><p>Strategies are needed to improve repopulation of decellularized lung scaffolds with stromal and functional epithelial cells. We demonstrate that decellularized mouse lungs recellularized in a dynamic low fluid shear suspension bioreactor, termed the rotating wall vessel (RWV), contained more cells with decreased apoptosis, increased proliferation and enhanced levels of total RNA compared to static recellularization conditions. These results were observed with two relevant mouse cell types: bone marrow-derived mesenchymal stromal (stem) cells (MSCs) and alveolar type II cells (C10). In addition, MSCs cultured in decellularized lungs under static but not bioreactor conditions formed multilayered aggregates. Gene expression and immunohistochemical analyses suggested differentiation of MSCs into collagen I-producing fibroblast-like cells in the bioreactor, indicating enhanced potential for remodeling of the decellularized scaffold matrix. In conclusion, dynamic suspension culture is promising for enhancing repopulation of decellularized lungs, and could contribute to remodeling the extracellular matrix of the scaffolds with subsequent effects on differentiation and functionality of inoculated cells.</p></div>", "links"=>[], "tags"=>["decellularized lungs", "decellularized mouse lungs recellularized", "Decellularized Lung Scaffolds", "fluid shear suspension bioreactor", "rwv", "alveolar type II cells", "msc", "Dynamic Suspension Culture Strategies", "mouse cell types", "rna", "decellularized scaffold matrix"], "article_id"=>1410049, "categories"=>["Biological Sciences"], "users"=>["Aurélie Crabbé", "Yulong Liu", "Shameema F. Sarker", "Nicholas R. Bonenfant", "Jennifer Barrila", "Zachary D. Borg", "James J. Lee", "Daniel J. Weiss", "Cheryl A. Nickerson"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0126846.s001", "https://dx.doi.org/10.1371/journal.pone.0126846.s002"], "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Recellularization_of_Decellularized_Lung_Scaffolds_Is_Enhanced_by_Dynamic_Suspension_Culture_/1410049", "title"=>"Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-05-11 03:10:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/2061615"], "description"=>"<p>Profiling of whole normal mouse lung tissue and MSC monolayers was performed as well. Cell nuclei are labeled in blue; markers of interest are labeled in green and are Fsp1 (panel A to E), collagen I (panel a to e), and osteopontin (panel aa to ee). White arrows point to multilayered cell aggregates, observed in static recellularization conditions and for this test condition profiling for both aggregates (A, a, aa) and single cells (B, b, bb) is presented. Since collagen I-positive cells showed higher signal intensity compared to that of the collagen I-positive scaffold, the background scaffold signal in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126846#pone.0126846.g005\" target=\"_blank\">Fig 5Ab and 5Ac</a> was removed for clarity. To demonstrate the collagen I-positive scaffolds, the background signal is only shown in Fig 5Aa. Magnifications are 400x or 630x. B: Alizarin red staining of decellularized lung scaffolds recellularized with MSCs in static (panels A, B) and bioreactor (panel C) conditions for 14 days. As a negative control, decellularized lungs that were not seeded with cells are presented (panel D). MSC monolayers differentiated along the osteoblastic lineage are included as positive control (panel E). Magnification is 200x or 630x. For each condition, images are representative of the entire lung.</p>", "links"=>[], "tags"=>["decellularized lungs", "decellularized mouse lungs recellularized", "Decellularized Lung Scaffolds", "fluid shear suspension bioreactor", "rwv", "alveolar type II cells", "msc", "Dynamic Suspension Culture Strategies", "mouse cell types", "rna", "decellularized scaffold matrix"], "article_id"=>1410047, "categories"=>["Biological Sciences"], "users"=>["Aurélie Crabbé", "Yulong Liu", "Shameema F. Sarker", "Nicholas R. Bonenfant", "Jennifer Barrila", "Zachary D. Borg", "James J. Lee", "Daniel J. Weiss", "Cheryl A. Nickerson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0126846.g005", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_Immunohistochemical_profiling_of_decellularized_lung_scaffolds_recellularized_with_MSCs_in_static_and_bioreactor_conditions_for_14_days_/1410047", "title"=>"A: Immunohistochemical profiling of decellularized lung scaffolds recellularized with MSCs in static and bioreactor conditions for 14 days.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-05-11 03:10:07"}

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{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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