Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes
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{"title"=>"Effect of storage temperature on structure and function of cultured human oral keratinocytes", "type"=>"journal", "authors"=>[{"first_name"=>"Rakibul", "last_name"=>"Islam", "scopus_author_id"=>"57197224515"}, {"first_name"=>"Catherine", "last_name"=>"Jackson", "scopus_author_id"=>"56147848400"}, {"first_name"=>"Jon R.", "last_name"=>"Eidet", "scopus_author_id"=>"54580903200"}, {"first_name"=>"Edward B.", "last_name"=>"Messelt", "scopus_author_id"=>"6603363792"}, {"first_name"=>"Rima Maria", "last_name"=>"Corraya", "scopus_author_id"=>"56719700300"}, {"first_name"=>"Torstein", "last_name"=>"Lyberg", "scopus_author_id"=>"7005002796"}, {"first_name"=>"May", "last_name"=>"Griffith", "scopus_author_id"=>"7102075338"}, {"first_name"=>"Darlene A.", "last_name"=>"Dartt", "scopus_author_id"=>"7006599175"}, {"first_name"=>"Tor P.", "last_name"=>"Utheim", "scopus_author_id"=>"16305461500"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84936976931", "doi"=>"10.1371/journal.pone.0128306", "pmid"=>"26052937", "issn"=>"19326203", "scopus"=>"2-s2.0-84936976931", "pui"=>"605098418"}, "id"=>"8de0b4bb-ee7b-3de3-8a27-3a2c5a0d2df7", "abstract"=>"PURPOSE/AIMS: To assess the effect of storage temperature on the viability, phenotype, metabolism, and morphology of cultured human oral keratinocytes (HOK).\\n\\nMATERIALS AND METHODS: Cultured HOK cells were stored in HEPES- and sodium bicarbonate-buffered Minimum Essential Medium (MEM) at nine temperatures in approximately 4°C increments from 4°C to 37°C for seven days. Cells were characterized for viability by calcein fluorescence, phenotype retention by immunocytochemistry, metabolic parameters (pH, glucose, lactate, and O2) within the storage medium by blood gas analysis, and morphology by scanning electron microscopy and light microscopy.\\n\\nRESULTS: Relative to the cultured, but non-stored control cells, a high percentage of viable cells were retained only in the 12°C and 16°C storage groups (85%±13% and 68%±10%, respectively). Expression of ABCG2, Bmi1, C/EBPδ, PCNA, cytokeratin 18, and caspase-3 were preserved after storage in the 5 groups between 4°C and 20°C, compared to the non-stored control. Glucose, pH and pO2 in the storage medium declined, whereas lactate increased with increasing storage temperature. Morphology was best preserved following storage of the three groups between 12°C, 16°C, and 20°C.\\n\\nCONCLUSION: We conclude that storage temperatures of 12°C and 16°C were optimal for maintenance of cell viability, phenotype, and morphology of cultured HOK. The storage method described in the present study may be applicable for other cell types and tissues; thus its significance may extend beyond HOK and the field of ophthalmology.", "link"=>"http://www.mendeley.com/research/effect-storage-temperature-structure-function-cultured-human-oral-keratinocytes-1", "reader_count"=>6, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Other"=>1, "Student > Master"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Other"=>1, "Student > Master"=>1}, "reader_count_by_subject_area"=>{"Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}}, "reader_count_by_country"=>{"South Korea"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2100108"], "description"=>"<p>Photomicrographs are representative of two independent samples. White cross (B) shows an intercellular space.</p>", "links"=>[], "tags"=>["storage medium", "storage temperature", "abcg", "hepes", "scanning electron microscopy", "mem", "blood gas analysis", "pcna", "MethodsCultured HOK cells"], "article_id"=>1440286, "categories"=>["Biological Sciences"], "users"=>["Rakibul Islam", "Catherine Jackson", "Jon R. Eidet", "Edward B. Messelt", "Rima Maria Corraya", "Torstein Lyberg", "May Griffith", "Darlene A. Dartt", "Tor P. Utheim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128306.g006", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Photomicrographs_of_cultured_HOK_stored_for_seven_days_at_nine_temperatures_were_captured_with_an_inverted_light_microscope_with_400x_magnification_A_J_/1440286", "title"=>"Photomicrographs of cultured HOK stored for seven days at nine temperatures were captured with an inverted light microscope with 400x magnification (A-J).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 03:16:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100119"], "description"=>"<p>List of the antibodies.</p>", "links"=>[], "tags"=>["storage medium", "storage temperature", "abcg", "hepes", "scanning electron microscopy", "mem", "blood gas analysis", "pcna", "MethodsCultured HOK cells"], "article_id"=>1440297, "categories"=>["Biological Sciences"], "users"=>["Rakibul Islam", "Catherine Jackson", "Jon R. Eidet", "Edward B. Messelt", "Rima Maria Corraya", "Torstein Lyberg", "May Griffith", "Darlene A. Dartt", "Tor P. Utheim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128306.t001", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_List_of_the_antibodies_/1440297", "title"=>"List of the antibodies.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-06-08 03:16:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100118"], "description"=>"<p>Cultured HOK cells not subjected to storage served as control. Photomicrographs are representative of two independent samples. The white arrow in (C) shows the apoptotic body. White arrow (I) shows artifacts due to sample preparation.</p>", "links"=>[], "tags"=>["storage medium", "storage temperature", "abcg", "hepes", "scanning electron microscopy", "mem", "blood gas analysis", "pcna", "MethodsCultured HOK cells"], "article_id"=>1440296, "categories"=>["Biological Sciences"], "users"=>["Rakibul Islam", "Catherine Jackson", "Jon R. Eidet", "Edward B. Messelt", "Rima Maria Corraya", "Torstein Lyberg", "May Griffith", "Darlene A. Dartt", "Tor P. Utheim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128306.g008", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Photomicrographs_of_cultured_HOK_stored_for_seven_days_at_nine_temperatures_were_captured_with_a_scanning_electron_microscope_with_6000x_magnification_A_J_/1440296", "title"=>"Photomicrographs of cultured HOK stored for seven days at nine temperatures were captured with a scanning electron microscope with 6000x magnification (A-J).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 03:16:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100095"], "description"=>"<p>(A) Control cells were CAM positive (green). Original magnification: 200x. (B) HOK were seeded in multidishes at different concentrations and incubated for two hours to ensure attachment. The CAM reagent was added to the cells for one hour, and CAM fluorescence was measured with a microplate fluorometer (excitation/emission filter pair 485/538nm).</p>", "links"=>[], "tags"=>["storage medium", "storage temperature", "abcg", "hepes", "scanning electron microscopy", "mem", "blood gas analysis", "pcna", "MethodsCultured HOK cells"], "article_id"=>1440273, "categories"=>["Biological Sciences"], "users"=>["Rakibul Islam", "Catherine Jackson", "Jon R. Eidet", "Edward B. Messelt", "Rima Maria Corraya", "Torstein Lyberg", "May Griffith", "Darlene A. Dartt", "Tor P. Utheim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128306.g001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Calcein_acetoxymethyl_ester_CAM_which_exclusively_stains_live_cells_was_used_to_analyze_cell_survival_/1440273", "title"=>"Calcein-acetoxymethyl ester (CAM), which exclusively stains live cells, was used to analyze cell survival.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 03:16:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100103"], "description"=>"<p>Freshly prepared medium served as control (N = 8). Error bars indicate standard error of the mean.</p>", "links"=>[], "tags"=>["storage medium", "storage temperature", "abcg", "hepes", "scanning electron microscopy", "mem", "blood gas analysis", "pcna", "MethodsCultured HOK cells"], "article_id"=>1440281, "categories"=>["Biological Sciences"], "users"=>["Rakibul Islam", "Catherine Jackson", "Jon R. Eidet", "Edward B. Messelt", "Rima Maria Corraya", "Torstein Lyberg", "May Griffith", "Darlene A. Dartt", "Tor P. Utheim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128306.g005", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_To_assess_the_effect_of_storage_temperature_on_HOK_metabolism_the_glucose_used_was_calculated_and_is_presented_in_relation_to_lactate_produced_/1440281", "title"=>"To assess the effect of storage temperature on HOK metabolism the glucose used was calculated and is presented in relation to lactate produced.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 03:16:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100120"], "description"=>"<p>The immunoreactivity was graded as 0 (undetectable), + (detectable in <1/4 of the cells), ++ (detectable in 1/4–1/2 of the cells), +++ (detectable in 1/2-3/4 of the cells) and ++++ (detectable in >3/4 of the cells). All scores were assigned at a magnification of 200x by experienced investigators.</p><p>List of the antibodies.</p>", "links"=>[], "tags"=>["storage medium", "storage temperature", "abcg", "hepes", "scanning electron microscopy", "mem", "blood gas analysis", "pcna", "MethodsCultured HOK cells"], "article_id"=>1440298, "categories"=>["Biological Sciences"], "users"=>["Rakibul Islam", "Catherine Jackson", "Jon R. Eidet", "Edward B. Messelt", "Rima Maria Corraya", "Torstein Lyberg", "May Griffith", "Darlene A. Dartt", "Tor P. Utheim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128306.t002", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_List_of_the_antibodies_/1440298", "title"=>"List of the antibodies.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-06-08 03:16:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100102"], "description"=>"<p>(N = 8 for stored samples and N = 5 for samples not subjected to storage (control)). * P < 0.05 compared to control. Error bars indicate standard error of the mean.</p>", "links"=>[], "tags"=>["storage medium", "storage temperature", "abcg", "hepes", "scanning electron microscopy", "mem", "blood gas analysis", "pcna", "MethodsCultured HOK cells"], "article_id"=>1440280, "categories"=>["Biological Sciences"], "users"=>["Rakibul Islam", "Catherine Jackson", "Jon R. Eidet", "Edward B. Messelt", "Rima Maria Corraya", "Torstein Lyberg", "May Griffith", "Darlene A. Dartt", "Tor P. Utheim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128306.g004", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_To_assess_the_effect_of_storage_temperature_on_HOK_metabolism_the_A_pH_in_the_storage_medium_and_B_partial_pressure_of_oxygen_pO_2_kPa_was_measured_by_a_blood_gas_analyzer_following_seven_days_of_storage_/1440280", "title"=>"To assess the effect of storage temperature on HOK metabolism the (A) pH in the storage medium and (B) partial pressure of oxygen (pO<sub>2</sub> kPa) was measured by a blood gas analyzer following seven days of storage.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 03:16:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100100"], "description"=>"<p>Photomicrographs are representative of four independent samples. Cell nuclei were counterstained with DAPI (blue). Original magnification: 200x.</p>", "links"=>[], "tags"=>["storage medium", "storage temperature", "abcg", "hepes", "scanning electron microscopy", "mem", "blood gas analysis", "pcna", "MethodsCultured HOK cells"], "article_id"=>1440278, "categories"=>["Biological Sciences"], "users"=>["Rakibul Islam", "Catherine Jackson", "Jon R. Eidet", "Edward B. Messelt", "Rima Maria Corraya", "Torstein Lyberg", "May Griffith", "Darlene A. Dartt", "Tor P. Utheim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128306.g003", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Photomicrographs_showing_immunostaining_of_A_ABCG2_red_B_Bmi_1_green_C_C_EBP_948_red_D_PCNA_red_E_CK18_green_and_F_cleaved_caspase_3_red_in_HOK_cultured_for_five_days_without_subsequent_storage_control_/1440278", "title"=>"Photomicrographs showing immunostaining of (A) ABCG2 (red), (B) Bmi-1 (green), (C) C/EBPδ (red), (D) PCNA (red), (E) CK18 (green), and (F) cleaved caspase-3 (red) in HOK cultured for five days without subsequent storage (control).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 03:16:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100114"], "description"=>"<p>Cultured HOK cells not subjected to storage served as control. Photomicrographs are representative of two independent samples. White arrow (B) shows cell shrinkage and the white arrow in (J) shows the detachment of the cells.</p>", "links"=>[], "tags"=>["storage medium", "storage temperature", "abcg", "hepes", "scanning electron microscopy", "mem", "blood gas analysis", "pcna", "MethodsCultured HOK cells"], "article_id"=>1440292, "categories"=>["Biological Sciences"], "users"=>["Rakibul Islam", "Catherine Jackson", "Jon R. Eidet", "Edward B. Messelt", "Rima Maria Corraya", "Torstein Lyberg", "May Griffith", "Darlene A. Dartt", "Tor P. Utheim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128306.g007", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Photomicrographs_of_cultured_HOK_stored_for_seven_days_at_nine_temperatures_were_captured_with_a_scanning_electron_microscope_with_550x_magnification_A_J_/1440292", "title"=>"Photomicrographs of cultured HOK stored for seven days at nine temperatures were captured with a scanning electron microscope with 550x magnification (A-J).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 03:16:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100096"], "description"=>"<p>The bar chart illustrates the percentage of viable cells remaining after storage compared to control cells. (N = 5) * <i>P</i> < 0.05 compared to the control. Error bars indicate standard error of the mean.</p>", "links"=>[], "tags"=>["storage medium", "storage temperature", "abcg", "hepes", "scanning electron microscopy", "mem", "blood gas analysis", "pcna", "MethodsCultured HOK cells"], "article_id"=>1440274, "categories"=>["Biological Sciences"], "users"=>["Rakibul Islam", "Catherine Jackson", "Jon R. Eidet", "Edward B. Messelt", "Rima Maria Corraya", "Torstein Lyberg", "May Griffith", "Darlene A. Dartt", "Tor P. Utheim"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128306.g002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cultured_HOK_were_stored_for_seven_days_at_nine_different_temperatures_and_viability_was_assessed_by_measuring_calcein_acetoxymethyl_ester_CAM_fluorescence_with_a_microplate_fluorometer_/1440274", "title"=>"Cultured HOK were stored for seven days at nine different temperatures and viability was assessed by measuring calcein-acetoxymethyl ester (CAM) fluorescence with a microplate fluorometer.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 03:16:49"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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