Mouse DRG Cell Line with Properties of Nociceptors
Publication Date
June 08, 2015
Journal
PLOS ONE
Authors
Ciara Doran, Jonathan Chetrit, Matthew C. Holley, David Grundy, et al
Volume
10
Issue
6
Pages
e0128670
DOI
https://dx.plos.org/10.1371/journal.pone.0128670
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0128670
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/26053673
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460156
Europe PMC
http://europepmc.org/abstract/MED/26053673
Scopus
84936862435
Mendeley
http://www.mendeley.com/research/mouse-drg-cell-line-properties-nociceptors
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Mendeley | Further Information

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Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2100943"], "description"=>"<p>Immunolabelling of MED17.11 cells cultured in permissive conditions for large T antigen expression. GFP transgene expression in MED17.11. Scale bar is 100 μm.</p>", "links"=>[], "tags"=>["marker NaV 1.8", "voltage gated sodium channel agonist", "TRPV 1 agonist capsaicin", "cell line MED 17.11", "DRG neurons", "MED 17.11 cells", "DRG neurons aid drug discovery", "mouse DRG Cell Line", "Robust calcium transients", "purinergic receptor agonist ATP", "DRG cell line"], "article_id"=>1440914, "categories"=>["Biological Sciences", "Science Policy"], "users"=>["Ciara Doran", "Jonathan Chetrit", "Matthew C. Holley", "David Grundy", "Mohammed A. Nassar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128670.g001", "stats"=>{"downloads"=>3, "page_views"=>396, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MED17_11_express_the_early_neuronal_markers_FOX3_NeuN_Isl1_and_Tuj1_and_can_be_transfected_with_GFP_/1440914", "title"=>"MED17.11 express the early neuronal markers FOX3 (NeuN), Isl1 and Tuj1, and can be transfected with GFP.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 04:12:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100944"], "description"=>"<p>A, Protocol for differentiation of MED17.11 cells. Bright-field images (B and C) of MED17.11, 3 days after the addition of differentiation medium. B, Low magnification images illustrate the high efficiency of morphological differentiation using the protocol shown in A. The small black arrow points to the rarer large diameter neurons. C, Higher power images showing the typical morphology of MED17.11 cells. The large black arrow points to an example of process branching. D, Frequency distribution of cell soma diameters (left) demonstrates the diversity of cell sizes following differentiation. E, Histogram showing change in soma diameter post differentiation (right) * = p<0.05, ** = p<0.001 (mean + S.E.M; Student’s unpaired two tailed t test). F, Immunofluorescence images of MED17.11, 3 days after the addition of differentiation medium. Both soma and processes of MED17.11 cells label with the neuronal marker, Tuj1, and the sensory neuron marker, Advillin. All scale bars are 50 μm.</p>", "links"=>[], "tags"=>["marker NaV 1.8", "voltage gated sodium channel agonist", "TRPV 1 agonist capsaicin", "cell line MED 17.11", "DRG neurons", "MED 17.11 cells", "DRG neurons aid drug discovery", "mouse DRG Cell Line", "Robust calcium transients", "purinergic receptor agonist ATP", "DRG cell line"], "article_id"=>1440915, "categories"=>["Biological Sciences", "Science Policy"], "users"=>["Ciara Doran", "Jonathan Chetrit", "Matthew C. Holley", "David Grundy", "Mohammed A. Nassar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128670.g002", "stats"=>{"downloads"=>2, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Morphological_differentiation_of_MED17_11_/1440915", "title"=>"Morphological differentiation of MED17.11.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 04:12:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100946"], "description"=>"<p>Left, mRNA analysis of proliferating and differentiated MED17.11 cells: “Undiff” refers to cells grown in proliferating conditions (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128670#sec002\" target=\"_blank\">methods</a>). “Diff” refers to cells grown in differentiating conditions for 7 days (day 9 of differentiation protocol in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128670#pone.0128670.g002\" target=\"_blank\">Fig 2</a>, also see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128670#sec002\" target=\"_blank\">methods</a>). A “+” indicates that the cell line was positive and a “-” indicates that the cell was negative for the corresponding marker. A”?” indicates that the marker was not tested in the given condition A. The “*” beside Runx1 indicates that this nociceptor marker was also tested after one day in differentiation conditions and was positive. LTM = low threshold mechanoreceptor, VGSC = voltage gated sodium channel. Right, immunocytochemical analysis of MED17.11 cells differentiated for 7 days. The cells showed immunoreactivity for the modality specific markers TrkA and TrkC (top) and the embryonic VGSC, Nav1.3 (bottom left). Live MED17.11 cells also strongly labelled with IB4 (bottom right). Scale bar is 50 μm.</p>", "links"=>[], "tags"=>["marker NaV 1.8", "voltage gated sodium channel agonist", "TRPV 1 agonist capsaicin", "cell line MED 17.11", "DRG neurons", "MED 17.11 cells", "DRG neurons aid drug discovery", "mouse DRG Cell Line", "Robust calcium transients", "purinergic receptor agonist ATP", "DRG cell line"], "article_id"=>1440917, "categories"=>["Biological Sciences", "Science Policy"], "users"=>["Ciara Doran", "Jonathan Chetrit", "Matthew C. Holley", "David Grundy", "Mohammed A. Nassar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128670.g003", "stats"=>{"downloads"=>3, "page_views"=>55, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_mRNA_and_Immunocytochemical_profiles_of_MED17_11_/1440917", "title"=>"mRNA and Immunocytochemical profiles of MED17.11.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 04:12:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/2100947"], "description"=>"<p>A, representative traces from fura-2 AM calcium imaging showing responses to compounds that excite DRG neurons following 3–6 days of differentiation. Dotted Lines indicate the time when the stimulus was applied. B, left, percentage of proliferative and differentiated cells responding to each compound (Fisher’s Exact two tailed test), * = p<0.05, ** = p<0.001, ***p<0.0001. Right, response amplitudes to each compound (mean + S.E.M). C, Percentage of MED cells responding when grown in non-permissive conditions with growth factors alone (NGF 100 ng/ml and GDNF 20 ng/ml) for 3 days. D, representative traces from fura-2 AM calcium imaging showing responses to inflammatory mediators following 3–6 days of differentiation. Dotted Lines indicate the time when the stimulus was applied. E, left, percentage of cells responding to each compound; right, response amplitudes to each compound (mean + S.E.M).</p>", "links"=>[], "tags"=>["marker NaV 1.8", "voltage gated sodium channel agonist", "TRPV 1 agonist capsaicin", "cell line MED 17.11", "DRG neurons", "MED 17.11 cells", "DRG neurons aid drug discovery", "mouse DRG Cell Line", "Robust calcium transients", "purinergic receptor agonist ATP", "DRG cell line"], "article_id"=>1440918, "categories"=>["Biological Sciences", "Science Policy"], "users"=>["Ciara Doran", "Jonathan Chetrit", "Matthew C. Holley", "David Grundy", "Mohammed A. Nassar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0128670.g004", "stats"=>{"downloads"=>4, "page_views"=>41, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differentiated_MED17_11_responds_to_compounds_that_excite_sensory_neurons_and_inflammatory_mediators_/1440918", "title"=>"Differentiated MED17.11 responds to compounds that excite sensory neurons and inflammatory mediators.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-08 04:12:37"}

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{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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