Impaired Expression of Prostaglandin E2 (PGE2) Synthesis and Degradation Enzymes during Differentiation of Immortalized Urothelial Cells from Patients with Interstitial Cystitis/Painful Bladder Syndrome
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Impaired Expression of Prostaglandin E2 (PGE2) Synthesis and Degradation Enzymes during Differentiation of Immortalized Urothelial Cells from Patients with Interstitial Cystitis/Painful Bladder Syndrome", "type"=>"journal", "authors"=>[{"first_name"=>"John O.", "last_name"=>"Marentette"}, {"first_name"=>"Robert E.", "last_name"=>"Hurst"}, {"first_name"=>"Jane", "last_name"=>"McHowat"}], "year"=>2015, "source"=>"PLOS ONE", "identifiers"=>{"issn"=>"1932-6203", "pmid"=>"26057882", "doi"=>"10.1371/journal.pone.0129466"}, "id"=>"5f97abd5-1ecd-3c3f-8117-13fb72cf482a", "abstract"=>"PURPOSE: The differentiated superficial cells of the urothelium restrict urine flow into the bladder wall. We have demonstrated that urothelial cells isolated from bladders of patients with interstitial cystitis/painful bladder syndrome (IC/PBS) fail to release PGE2 in response to tryptase. This study examines the expression of PGE2 synthesis and degradation enzymes in urothelial cells during differentiation.\\n\\nMATERIALS AND METHODS: We measured immunoprotein expression of cyclooxygenase-2 (COX-2), prostaglandin E2 synthase (PGES) and 15-hydroxyprostaglandin dehydrogenase (PGDH) in human urothelial cells and in immortalized urothelial cells isolated from the bladders of IC/PBS patients or normal subjects during stratification and differentiation produced by increased calcium and fetal bovine serum (Ca/FBS) in the culture medium for 1, 3 and 7 days.\\n\\nRESULTS: PGES immunoprotein expression increased during differentiation in normal and IC/PBS urothelial cells. COX-2 expression also increased in cells from normal patients following differentiation. Remarkably, no COX-2 expression was detectable in urothelial cells isolated from 3 out of 4 IC/PBS patients. PGDH immunoprotein expression decreased in normal cells after 1 and 3 days of Ca/FBS addition, but returned to normal after 7 days. PGDH expression was unchanged during differentiation at 1 and 3 days, but was more than 2-fold higher at 7 days compared to day 0 in the IC/PBS cells. Urothelial cells isolated from IC/PBS patients demonstrated no PGE2 release in response to tryptase under any of the experimental conditions studied.\\n\\nCONCLUSIONS: Taken together, our results indicate that PGE2 release is compromised during stratification and differentiation in IC/PBS urothelium and may contribute to impaired barrier function.", "link"=>"http://www.mendeley.com/research/impaired-expression-prostaglandin-e2-pge2-synthesis-degradation-enzymes-during-differentiation-immor", "reader_count"=>6, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Doctoral Student"=>1, "Researcher"=>2, "Student > Master"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Doctoral Student"=>1, "Researcher"=>2, "Student > Master"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Agricultural and Biological Sciences"=>2, "Medicine and Dentistry"=>1, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84936872626"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84936872626?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84936872626&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84936872626&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84936872626", "dc:identifier"=>"SCOPUS_ID:84936872626", "eid"=>"2-s2.0-84936872626", "dc:title"=>"Impaired expression of prostaglandin E<inf>2</inf> (PGE<inf>2</inf>) synthesis and degradation enzymes during differentiation of immortalized urothelial cells from patients with interstitial cystitis/painful bladder syndrome", "dc:creator"=>"Marentette J.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"6", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-06-09", "prism:coverDisplayDate"=>"9 June 2015", "prism:doi"=>"10.1371/journal.pone.0129466", "citedby-count"=>"2", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"St. Louis University School of Medicine", "affiliation-city"=>"St Louis", "affiliation-country"=>"United States"}], "pubmed-id"=>"26057882", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0129466", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0129466", "share_count"=>1, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>1}

Counter

  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"48", "xml_views"=>"12", "html_views"=>"305"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"83"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"3", "html_views"=>"15"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"1"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2103729"], "description"=>"<p>Upper panel: Representative immunoblots of PGES and actin expression in human urothelial cells (HUC) and immortalized urothelial cells from normal and IC/PBS patients. Lower panel: PGES immunoprotein was compared to actin for each sample and normalized to 1 for samples at Day 0. PGES immunoprotein changes were analyzed in three separate cells cultures of human urothelial cells (HUC, n = 3) or immortalized urothelial cells from normal (n = 4) or IC/PBS patients (n = 4). Data shown are mean ± SEM. ** p<0.01 when compared to day 0.</p>", "links"=>[], "tags"=>["differentiation", "cox", "PGDH immunoprotein expression", "PGE 2 synthesis", "7 days.ResultsPGES immunoprotein expression", "prostaglandin E 2", "PGE 2 release", "Immortalized Urothelial Cells", "release PGE 2", "7 days", "ic", "3 days", "urothelial cells"], "article_id"=>1443648, "categories"=>["Biological Sciences"], "users"=>["John O. Marentette", "Robert E. Hurst", "Jane McHowat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0129466.g005", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_15_hydroxyprostaglandin_dehydrogenase_PGDH_in_human_urothelial_cells_HUC_and_immortalized_urothelial_cells_from_normal_and_IC_PBS_patients_after_Ca_FBS_addition_/1443648", "title"=>"Expression of 15-hydroxyprostaglandin dehydrogenase (PGDH) in human urothelial cells (HUC) and immortalized urothelial cells from normal and IC/PBS patients after Ca/FBS addition.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-09 02:44:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/2103726"], "description"=>"<p>PGE<sub>2</sub> release was measured in cells grown to confluence (Day 0, filled circles) or in cells stratified by the addition of FBS and calcium for 3 days (Day 3, open circles). Data shown are means ± SEM for triplicate experiments using cells isolated from 4 normal and 4 IC/PBS patients. **p<0.01,when compared to unstimulated controls.</p>", "links"=>[], "tags"=>["differentiation", "cox", "PGDH immunoprotein expression", "PGE 2 synthesis", "7 days.ResultsPGES immunoprotein expression", "prostaglandin E 2", "PGE 2 release", "Immortalized Urothelial Cells", "release PGE 2", "7 days", "ic", "3 days", "urothelial cells"], "article_id"=>1443645, "categories"=>["Biological Sciences"], "users"=>["John O. Marentette", "Robert E. Hurst", "Jane McHowat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0129466.g002", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PGE_2_release_in_immortalized_urothelial_cells_isolated_from_normal_subjects_upper_panel_primary_human_urothelial_cells_HUC_middle_panel_and_IC_PBS_patients_lower_panel_after_20_ng_ml_tryptase_stimulation_/1443645", "title"=>"PGE<sub>2</sub> release in immortalized urothelial cells isolated from normal subjects (upper panel), primary human urothelial cells (HUC, middle panel) and IC/PBS patients (lower panel) after 20 ng/ml tryptase stimulation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-09 02:44:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/2103727"], "description"=>"<p>Upper panel: Representative immunoblots of COX-2 and actin expression in human urothelial cells (HUC) and immortalized urothelial cells from normal and IC/PBS patients. Lower panel: COX-2 immunoprotein was compared to actin for each sample and normalized to 1 for samples at Day 0. COX-2 immunoprotein changes were analyzed in three separate cells cultures of human urothelial cells (HUC, n = 3) or immortalized urothelial cells from normal (n = 4) or IC/PBS patients (n = 4, denoted in figure as #1–4). Data shown are mean ± SEM. ** p<0.01 when compared to day 0.</p>", "links"=>[], "tags"=>["differentiation", "cox", "PGDH immunoprotein expression", "PGE 2 synthesis", "7 days.ResultsPGES immunoprotein expression", "prostaglandin E 2", "PGE 2 release", "Immortalized Urothelial Cells", "release PGE 2", "7 days", "ic", "3 days", "urothelial cells"], "article_id"=>1443646, "categories"=>["Biological Sciences"], "users"=>["John O. Marentette", "Robert E. Hurst", "Jane McHowat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0129466.g003", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_cyclooxygenase_2_COX_2_in_human_urothelial_cells_and_immortalized_urothelial_cells_from_normal_and_IC_PBS_patients_after_Ca_FBS_addition_/1443646", "title"=>"Expression of cyclooxygenase-2 (COX-2) in human urothelial cells, and immortalized urothelial cells from normal and IC/PBS patients after Ca/FBS addition.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-09 02:44:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/2103724"], "description"=>"<p>(A) Light micrographs were taken of cultured human urothelial cells (HUC), immortalized urothelial cells from a normal patient (Normal) or an IC/PBS patient (IC/BPS) that had been stained with toluidine blue. (B) Occludin expression in stratified urothelial cells after 3 days of Ca/FBS addition. (C) Development of trans epithelial electrical resistance (TEER) over 14 days.</p>", "links"=>[], "tags"=>["differentiation", "cox", "PGDH immunoprotein expression", "PGE 2 synthesis", "7 days.ResultsPGES immunoprotein expression", "prostaglandin E 2", "PGE 2 release", "Immortalized Urothelial Cells", "release PGE 2", "7 days", "ic", "3 days", "urothelial cells"], "article_id"=>1443643, "categories"=>["Biological Sciences"], "users"=>["John O. Marentette", "Robert E. Hurst", "Jane McHowat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0129466.g001", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Urothelial_cells_differentiate_into_a_stratified_epithelial_culture_consisting_of_thin_tightly_apposed_apical_superficial_cells_and_more_loosely_connected_underlying_cells_/1443643", "title"=>"Urothelial cells differentiate into a stratified epithelial culture consisting of thin, tightly apposed apical superficial cells and more loosely connected underlying cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-09 02:44:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/2103728"], "description"=>"<p>Upper panel: Representative immunoblots of PGES and actin expression in human urothelial cells (HUC) and immortalized urothelial cells from normal and IC/PBS patients. Lower panel: PGES immunoprotein was compared to actin for each sample and normalized to 1 for samples at Day 0. PGES immunoprotein changes were analyzed in three separate cells cultures of human urothelial cells (HUC, n = 3) or immortalized urothelial cells from normal (n = 4) or IC/PBS patients (n = 4). Data shown are mean ± SEM. ** p<0.01 when compared to day 0.</p>", "links"=>[], "tags"=>["differentiation", "cox", "PGDH immunoprotein expression", "PGE 2 synthesis", "7 days.ResultsPGES immunoprotein expression", "prostaglandin E 2", "PGE 2 release", "Immortalized Urothelial Cells", "release PGE 2", "7 days", "ic", "3 days", "urothelial cells"], "article_id"=>1443647, "categories"=>["Biological Sciences"], "users"=>["John O. Marentette", "Robert E. Hurst", "Jane McHowat"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0129466.g004", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_prostaglandin_E_synthase_PGES_in_human_urothelial_cells_and_immortalized_urothelial_cells_from_normal_and_IC_PBS_patients_after_Ca_FBS_addition_/1443647", "title"=>"Expression of prostaglandin E synthase (PGES) in human urothelial cells, and immortalized urothelial cells from normal and IC/PBS patients after Ca/FBS addition.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-09 02:44:59"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"24", "full-text"=>"21", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"19", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"23", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"25", "full-text"=>"48", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"26", "full-text"=>"31", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts